Article
Cell Biology
Khaled Zubeidat, Yasmin Jaber, Yasmin Saba, Or Barel, Reem Naamneh, Yasmin Netanely, Yael Horev, Luba Eli-berchoer, Amjad Shhadeh, Omri Yosef, Eliran Arbib, Gili Betser-Cohen, Chen Nadler, Hagit Shapiro, Eran Elinav, Doron J. Aframian, Asaf Wilensky, Avi-Hai Hovav
Summary: This study investigates the postnatal mechanisms in the salivary glands that establish immunity. High levels of neutrophils and transferred maternal IgG are present temporarily in neonatal murine salivary glands, independent of the microbiota. During weaning, neutrophils, FcRn, and IgG decrease while pIgR is upregulated in a GAS6-dependent manner, independent of the microbiota. Salivary IgA production begins following weaning and requires CD4-help, IL-17, and the microbiota. This study suggests that salivary glands play a role as an immune inductive site.
Article
Oncology
Koen A. Marijt, Lisa Griffioen, Laura Blijleven, Sjoerd. H. van der Burg, Thorbald van Hall
Summary: TEIPP is a novel category of cancer antigens that emerge on cancers with functional loss of peptide pump TAP, evoking CD8 T cell immune response. By studying LRPAP1(21-30)-specific CD8 T cells, it was found that replacing the serine anchor with valine in the signal sequence enhances HLA-A2 binding affinity and T cell stimulation.
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2022)
Article
Immunology
Gaetan Barbet, Priyanka Nair-Gupta, Michael Schotsaert, Stephen T. Yeung, Julien Moretti, Fabian Seyffer, Giorgi Metreveli, Thomas Gardner, Angela Choi, Domenico Tortorella, Robert Tampe, Kamal M. Khanna, Adolfo Garcia-Sastre, J. Magarian Blander
Summary: The study explains how viruses target host cell antigen-processing pathways, focusing on MHC-I molecules and the re-routing of ERGIC-resident MHC molecules to phagosomal vesicles for cross-presentation. It suggests that even without TAP, protective CD8(+) T cells can still be mobilized during viral infection, highlighting a noncanonical cross-presentation pathway.
Article
Immunology
Ella R. Shepard, Anja Wegner, Elaine V. Hill, Bronwen R. Burton, Sarah Aerts, Evelien Schurgers, Brecht Hoedemaekers, Sky T. H. Ng, Heather B. Streeter, Lotta Jansson, David C. Wraith
Summary: Immunotherapy with apitopes targeting autoreactive CD4(+) T cells has shown to modulate autoimmune diseases progression by inducing tolerance and regulating cellular activity in the immune system.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Courtney K. Anderson, Shanelle P. Reilly, Laurent Brossay
Summary: IL-12, IL-18, and TLRs are dispensable for TCR activation pathway in iNKT cells, but are essential during murine CMV infection. There is an intrinsic requirement for IL-18 signaling by splenic iNKT cells but not liver iNKT cells. The exact contributions of these components for TCR-dependent and -independent activation pathways in iNKT cells are still unclear.
JOURNAL OF IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Oanh Tran, Silke Kerruth, Catherine Coates, Hansween Kaur, Camillo Peracchia, Tom Carter, Katalin Torok
Summary: This study investigates the interaction of Ca2+/calmodulin (Ca2+/CaM) with connexins (Cx) and provides insights into the regulation of gap junction function. The CL2 peptides of different Cx isoforms were found to have high affinity for Ca2+/CaM and also bind to apo-CaM. Furthermore, conformational changes in the CL2 domain were observed, suggesting its involvement in the hexameric gap junction. Ca2+/CaM was shown to inhibit gap junction permeability in a dose-dependent manner.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Marwa Eletreby, Lena Thiessen, Adrian Prager, Ilija Brizic, Jelena Materljan, Lucie Kubic, Katharina Jaeger, Krizan Jurinovic, Josipa Jerak, Karsten Krey, Barbara Adler
Summary: Cytomegaloviruses encode immunomodulatory proteins including chemokines, which play a crucial role in viral cell tropism and immune responses. Understanding the functions of these proteins is important for developing intervention strategies during herpesvirus infections.
Article
Microbiology
Gregory R. Sowd, Jiong Shi, Ashley R. Fulmer, Christopher Aiken
Summary: This study identifies surfaces on the HIV-1 capsid protein (CA) that modulate capsid stability in target cells and proposes targeting these surfaces with inhibitors to enhance the efficacy of lenacapavir.
Article
Chemistry, Multidisciplinary
Bing Xiao, Hongxia Xu, Xiaodan Xu, Yixuan Pan, Xueying Shi, Pengcheng Yuan, Nigel K. H. Slater, Wenjing Sun, Jianbin Tang, Youqing Shen, Jianqing Gao
Summary: The research team developed a multifunctional nanoassembly called FMMC, which can improve the immune characteristics of tumor cells and the tumor microenvironment. FMMC can induce immunogenic cell death, activate signaling pathways, enhance sensitivity to PD-1 therapy, and monitor tumor growth using MRI.
Article
Biochemistry & Molecular Biology
Zhilin Hu, Xiao-Lu Teng, Tianyu Zhang, Xiaoyan Yu, Rui Ding, Jing Yi, Liufu Deng, Zhengting Wang, Qiang Zou
Summary: Oxidative stress plays a crucial role in regulating STING-dependent DC antitumor function, with SENP3 directing this process. Deletion of SENP3 in DCs drives tumor progression and weakens the signaling of STING, resulting in reduced antitumor immune responses.
Article
Immunology
Dominika Borowska, Samantha Sives, Lonneke Vervelde, Kate M. Sutton
Summary: This study analyzes chicken macrophages and dendritic cells, revealing differences in phenotype, function, and transcription. The BM cultures with CSF2 and IL-4 tend to represent cells of the macrophage lineage rather than conventional dendritic cells.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Astrid Sissel Jorgensen, Emma Probst Brandum, Jeppe Malthe Mikkelsen, Klaudia A. Orfin, Ditte Rahbaek Boilesen, Kristoffer Lihme Egerod, Natasha A. Moussouras, Frederik Vilhardt, Pawel Kalinski, Per Basse, Yen-Hsi Chen, Zhang Yang, Michael B. Dwinell, Brian F. Volkman, Christopher T. Veldkamp, Peter Johannes Holst, Katharina Lahl, Christoffer Knak Goth, Mette Marie Rosenkilde, Gertrud Malene Hjorto
Summary: C21TP released from CCL21 significantly enhances the activity of the chemokine, independent of GAG binding but dependent on O-glycosylations in the CCR7 N-terminus. This boost in activity suggests a potential mechanism for improved chemokine-receptor interaction.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Multidisciplinary Sciences
Christin Pohl, Gregory Effantin, Eaazhisai Kandiah, Sebastian Meier, Guanghong Zeng, Werner Streicher, Dorotea Raventos Segura, Per H. Mygind, Dorthe Sandvang, Line Anker Nielsen, Gunther H. J. Peters, Guy Schoehn, Christoph Mueller-Dieckmann, Allan Noergaard, Pernille Harris
Summary: This study reports the cryo-EM structure of a triple-mutant of the antimicrobial peptide plectasin, which assembles into helical non-amyloid fibrils. The fibril formation is reversible and pH- and concentration-dependent. This finding reveals that alpha/beta proteins can naturally assemble into fibrils.
NATURE COMMUNICATIONS
(2022)
Article
Biochemical Research Methods
Honglan Li, Seungjin Na, Kyu-Baek Hwang, Eunok Paek
Summary: TIDD successfully eliminates the need for optimal feature engineering for each database search tool, making it directly applicable to any database search results. It performs similarly or better than Percolator in terms of peptide identification and offers a user-friendly graphical interface for convenience.
BMC BIOINFORMATICS
(2022)
Article
Biochemistry & Molecular Biology
Fabian Schorn, J. Paul Werthenbach, Mattes Hoffmann, Mila Daoud, Johanna Stachelscheid, Lars M. Schiffmann, Ximena Hildebrandt, Su Ir Lyu, Nieves Peltzer, Alexander Quaas, Domagoj Vucic, John Silke, Manolis Pasparakis, Hamid Kashkar
Summary: This study established a mouse model with mutated cIAP1/2, revealing the regulation of TNF signaling by cIAPs and their independent functions from RIPK1 and RIPK3. The study provides important molecular insights into TNF signaling.
Article
Endocrinology & Metabolism
Claire-Marine Berat, Sebastian Montealegre, Arnaud Wiedemann, Malou Le Corronc Nuzum, Amelie Blondel, Hugo Debruge, Aline Cano, Brigitte Chabrol, Celia Hoebeke, Michel Polak, Athanasia Stoupa, Francois Feillet, Stephanie Torre, Nathalie Boddaert, Henri Bruel, Magalie Barth, Lena Damaj, Marie-Therese Abi-Warde, Alexandra Afenjar, Jean-Francois Benoist, Marine Madrange, Laure Caccavelli, Perrine Renard, Arnaud Hubas, Patrick Nusbaum, Clement Pontoizeau, Stephanie Gobin, Peter van Endert, Chris Ottolenghi, Alice Maltret, Pascale de Lonlay
Summary: TANGO2 disease is a severe inherited disorder characterized by neurodevelopmental delay, acute metabolic crises, and hypothyroidism, with significant intrafamilial clinical variability. Triggers of metabolic crises in TANGO2 patients were identified, while primary mitochondrial defects are unlikely to be the cause of the disease.
JOURNAL OF INHERITED METABOLIC DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Corinna A. Kulicke, Erica De Zan, Zeynep Hein, Claudia Gonzalez-Lopez, Swapnil Ghanwat, Natacha Veerapen, Gurdyal S. Besra, Paul Klenerman, John C. Christianson, Sebastian Springer, Sebastian M. Nijman, Vincenzo Cerundolo, Mariolina Salio
Summary: The MR1-MAIT cell axis is involved in various diseases, and this study discovered that ATP13A1 in the endoplasmic reticulum is a key factor in MR1 surface expression.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Letter
Biochemistry & Molecular Biology
Sebastian Springer
MOLECULAR IMMUNOLOGY
(2022)
Article
Genetics & Heredity
Sebastian Montealegre, Elise Lebigot, Hugo Debruge, Norma Romero, Benedicte Heron, Pauline Gaignard, Antoine Legendre, Apolline Imbard, Stephanie Gobin, Emmanuelle Lacene, Patrick Nusbaum, Arnaud Hubas, Isabelle Desguerre, Aude Servais, Pascal Laforet, Peter van Endert, Francois Jerome Authier, Cyril Gitiaux, Pascale de Lonlay
Summary: This study characterizes the clinical and biological traits of two individuals with variants in ISCU and FDX2, finding that the individual with FDX2 variants is more severely affected.
NEUROLOGY-GENETICS
(2022)
Article
Cell Biology
Cindy Dirscherl, Sara Loechte, Zeynep Hein, Janine-Denise Kopicki, Antonia Regina Harders, Noemi Linden, Andreas Karner, Johannes Preiner, Julian Weghuber, Maria Garcia-Alai, Charlotte Uetrecht, Martin Zacharias, Jacob Piehler, Peter Lanzerstorfer, Sebastian Springer
Summary: MHC-I FHC dimers are the prevalent species at the plasma membrane, with a tendency to cluster into higher order oligomers. This clustering leads to a moderate decrease in diffusion coefficient.
JOURNAL OF CELL SCIENCE
(2022)
Article
Cell Biology
Phillip Pymm, Stefan Tenzer, Edmund Wee, Mirjana Weimershaus, Anne Burgevin, Simon Kollnberger, Jan Gerstoft, Tracy M. Josephs, Kristin Ladell, James E. McLaren, Victor Appay, David A. Price, Lars Fugger, John I. Bell, Hansjoerg Schild, Peter van Endert, Maria Harkiolaki, Astrid K. N. Iversen
Summary: This study discovers that the cytotoxic T lymphocyte (CTL) and natural killer (NK) cell responses to a specific epitope in the human immunodeficiency virus are associated with enhanced immune control. The length and mutations of the epitope influence the CTL and NK cell responses, leading to viral escape.
Article
Oncology
Lucillia Bezu, Alejandra Wu Chuang, Allan Sauvat, Juliette Humeau, Wei Xie, Giulia Cerrato, Peng Liu, Liwei Zhao, Shuai Zhang, Julie Le Naour, Jonathan Pol, Peter van Endert, Oliver Kepp, Fabrice Barlesi, Guido Kroemer
Summary: This study investigates the anticancer effects of six local anesthetics used in clinical practice. The results suggest that local anesthetics induce immunogenic stress responses in cancer cells, leading to antitumor effects.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Article
Biology
Janine-Denise Kopicki, Ankur Saikia, Stephan Niebling, Christian Guenther, Raghavendra Anjanappa, Maria Garcia-Alai, Sebastian Springer, Charlotte Uetrecht
Summary: The authors present a sensitive and rapid method to determine the binding strength of MHC class 1 peptide complexes using native mass spectrometry.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biophysics
Ambroise Marcais, Nizar Mahlaoui, Benedicte Neven, Fanny Lanternier, Emilie Catherinot, Helene Salvator, Morgane Cheminant, Maxime Jeljeli, Vahid Asnafi, Peter van Endert, Louis-Jean Couderc, Olivier Lortholary, Capucine Picard, Despina Moshous, Olivier Hermine, Alain Fischer, Felipe Suarez
Summary: Allo-HSCT is an effective and safe treatment for young adults with primary immunodeficiency disease (PID), with acceptable toxicity and clinical improvement.
BONE MARROW TRANSPLANTATION
(2022)
Article
Chemistry, Medicinal
Dionisios Vourloumis, Ioannis Mavridis, Alexandros Athanasoulis, Ioannis Temponeras, Despoina Koumantou, Petros Giastas, Anastasia Mpakali, Victoria Magrioti, Jacqueline Leib, Peter van Endert, Efstratios Stratikos, Athanasios Papakyriakou
Summary: A highly selective synthetic method for functionalization of the alpha-hydroxy-beta-amino acid scaffold of an aminopeptidase inhibitor has been discovered. Structural studies revealed that interactions with the GAMEN loop play a key role in the potency and selectivity of the inhibitor. This study suggests that alpha-hydroxy-beta-amino acid derivatives could serve as useful drug leads for aminopeptidases.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Cell Biology
Lucy C. Walters, Daniel Rozbesky, Karl Harlos, Max Quastel, Hong Sun, Sebastian Springer, Robert P. Rambo, Fiyaz Mohammed, E. Yvonne Jones, Andrew J. McMichael, Geraldine M. Gillespie
Summary: MHC-E regulates NK cells by displaying VL9 signal peptides and interacts with CD8(+) T cells through presenting pathogen-derived peptides. Three unique amino acids in MHC-E allow the binding of both VL9 and non-VL9 peptides, leading to immune discrimination.
Article
Biochemistry & Molecular Biology
Ankur Saikia, Andries Hadeler, Pranathi Prasad, Martin Zacharias, Sebastian Springer
Summary: This study investigates the importance of peptide exchange in immunodominance and uncovers the mechanism of peptide exchange under low pH conditions. The results demonstrate that low pH triggers peptide exchange and provide insights into the individual steps of the exchange reaction.
Editorial Material
Immunology
Jonas Kuiper, Peter van Endert
Summary: The Black Death, caused by Yersinia pestis infection, was a devastating pandemic in the 14th century that posed a significant challenge to human immune defenses. A recent study suggests that a genetic variant in an antigen-processing gene may have provided survival advantage during the plague and underwent rapid genomic selection in Europeans.
TRENDS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ayse Yilmaz, Chiara Guerrera, Emmanuelle Waeckel-Enee, Joanna Lipecka, Barbara Bertocci, Peter van Endert
Summary: Insulin-degrading enzyme (IDE) is mainly localized in the cytosol and has additional functions beyond insulin degradation. It interacts with multiple mitochondrial ribosomal proteins and proteins involved in mitochondrial complex synthesis and assembly. The mitochondrial interactomes of wild type and mutant IDE are similar and do not reveal proteolytic IDE substrates. IDE may have non-proteolytic functions in mitochondria, acting as a chaperone and contributing to protein homeostasis and stress responses.
Article
Biology
Katharina Hartman, Guido Steiner, Michel Siegel, Cary M. Looney, Timothy P. Hickling, Katharine Bray-French, Sebastian Springer, Celine Marban-Doran, Axel Ducret
Summary: This study investigates the identification of T cell epitopes in immune responses. The use of multiple antibody clones in an immunoprecipitation strategy improves the detection of compound-specific peptides. Expanding the assay can enhance the predictability of additional potential T cell epitopes.