Article
Immunology
Xiaolong Wu, Amit Sharma, Johannes Oldenburg, Hans Weiher, Markus Essler, Dirk Skowasch, Ingo G. H. Schmidt-Wolf
Summary: CIK cells are a promising candidate for immunotherapy due to their convenient and relatively inexpensive expansion capability. NKG2D plays a crucial role in CIK cell-mediated antitumor activity, with NKG2D engagement alone being sufficient to activate CIK cells. The role of 2B4 in CIK cells is still unclear, but it provides limited synergy with NKG2D in certain aspects. Additional research is needed to optimize the functional potential of CIK cells for cancer therapy.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Medicine, Research & Experimental
Haote Han, Yanhui Yang, Zhipan Wu, Bing Liu, Lijun Dong, Hongwei Deng, Jingkui Tian, Hetian Lei
Summary: Capilliposide B (CPS-B), a novel oleanane triterpenoid saponin derived from Lysimachia capillipes Hemsl, was found to inhibit VEGF-induced angiogenesis in HRECs by specifically targeting VEGFR2 and downstream signaling enzymes. This compound effectively blocked VEGF-stimulated proliferation, migration, and tube formation of HRECs, suggesting its potential as a prophylactic for angiogenesis-associated diseases such as proliferative diabetic retinopathy.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Immunology
Helene Jamann, Qiao-Ling Cui, Haritha L. Desu, Florian Pernin, Olivier Tastet, Alexandre Halaweh, Negar Farzam-kia, Victoria Hannah Mamane, Oumarou Ouedraogo, Aurelie Cleret-Buhot, Audrey Daigneault, Renaud Balthazard, Wendy Klement, Florent Lemaitre, Nathalie Arbour, Jack Antel, Jo Anne Stratton, Catherine Larochelle
Summary: This study reveals that Th17-polarized cells form biologically significant contacts with OLs and exert direct toxicity by releasing granzyme B in multiple sclerosis.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Ayla A. Wahid, Rhys W. Dunphy, Alex Macpherson, Beth G. Gibson, Liudmila Kulik, Kevin Whale, Catherine Back, Thomas M. Hallam, Bayan Alkhawaja, Rebecca L. Martin, Ingrid Meschede, Maisem Laabei, Alastair D. G. Lawson, V. Michael Holers, Andrew G. Watts, Susan J. Crennell, Claire L. Harris, Kevin J. Marchbank, Jean M. H. van den Elsen
Summary: The cleavage of C3 to C3a and C3b leads to the formation of C3b dimers, which can modulate B cell activation and influence tolerogenic pathways. Understanding the functions of C3d(g) dimers could enhance our knowledge of complement in the fluid phase and potentially inform the development of novel therapies for immune system disorders in the future.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Lydia Ogrodzinski, Simon Platt, Joelle Goulding, Cameron Alexander, Tracy D. Farr, Jeanette Woolard, Stephen J. Hill, Laura E. Kilpatrick
Summary: E-selectin is a protein expressed on endothelial cells in response to inflammatory cytokines, and it plays a role in leukocyte rolling and extravasation. This study used CRISPR-Cas9 genome editing and NanoLuc Binary Technology (NanoBiT) to tag endogenous E-selectin in human umbilical vein endothelial cells (HUVECs) and monitor its expression in real time. The combination of NanoBiT and CRISPR-Cas9 gene editing provides a powerful tool for monitoring dynamic changes in E-selectin expression on cell surfaces, which can contribute to the discovery of drugs targeting this important inflammatory protein.
Article
Cell Biology
Mark F. Santos, Germana Rappa, Simona Fontana, Jana Karbanova, Feryal Aalam, Derek Tai, Zhiyin Li, Marzia Pucci, Riccardo Alessandro, Chikao Morimoto, Denis Corbeil, Aurelio Lorico
Summary: Intercellular communication between cancer cells and healthy cells in the tumor microenvironment plays a crucial role in cancer progression and metastasis. Extracellular vesicles (EVs) have emerged as novel mediators in this communication. In this study, the researchers developed a monovalent Fab fragment antibody targeting CD9 tetraspanin and demonstrated its effectiveness in blocking the internalization of melanoma cell-derived EVs and the transfer of their cargo proteins. The study also showed the potential of this anti-CD9 Fab antibody in inhibiting the phenotypic transformation and migration of colorectal cancer cells. Intercepting EV-mediated communication in the tumor niche with this antibody could lead to new anti-cancer therapeutic strategies.
Article
Multidisciplinary Sciences
Markus Lechner, Thomas Engleitner, Tea Babushku, Marc Schmidt-Supprian, Roland Rad, Lothar J. Strobl, Ursula Zimber-Strobl
Summary: FoB and MZB cells are functionally distinct mature B cell populations in the spleen, which can be re-programmed through Notch2 activation. The study demonstrates plasticity between these cell types and suggests a potential pathway for MZB cell development in vivo, driven by a singular signaling event.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Carsten Riether, Ramin Radpour, Nils M. Kallen, Damian T. Burgin, Chantal Bachmann, Christian M. Schurch, Ursina Luthi, Miroslav Arambasic, Sven Hoppe, Christoph E. Albers, Gabriela M. Baerlocher, Adrian F. Ochsenbein
Summary: CD93 signaling plays a critical role in regulating self-renewal and proliferation of stem cells, promoting gene transcription via SCY1-like pseudokinase 1 in murine and human LSCs. The anti-emetic agent metoclopramide efficiently blocks CD93 signaling, reducing LSCs numbers and prolonging survival in CML mice by downregulating stemness and proliferation pathways.
Article
Cell Biology
Shengliang Gu, Fahui Liu, Xueheng Xie, Meng Ding, Zhen Wang, Xiaoyan Xing, Tianbao Xiao, Xiaobo Sun
Summary: The study aimed to investigate the biological functions and prognostic value of the LEF-1-mediated Wnt/β-catenin pathway in colon cancer (CC). Beta-sitosterol was found to effectively suppress CC cell growth and migration by down-regulating LEF-1 and disrupting the Wnt/β-catenin pathway. These results suggest that beta-sitosterol can be used as a potential treatment for CC by targeting LEF-1.
CELLULAR SIGNALLING
(2023)
Article
Virology
Toshitada Takemori, Akiko Sugimoto-Ishige, Hironori Nishitsuji, Yushi Futamura, Michishige Harada, Tomomi Kimura-Someya, Takehisa Matsumoto, Teruki Honma, Miho Tanaka, Masami Yaguchi, Kyoichi Isono, Haruhiko Koseki, Hiroyuki Osada, Daiki Miki, Takashi Saito, Takashi Tanaka, Takehiro Fukami, Toshio Goto, Mikako Shirouzu, Kunitada Shimotohno, Kazuaki Chayama
Summary: Entry inhibitors are being developed to enhance the treatment of HBV patients with oral nucleoside/nucleotide analogues (NA). Therapeutic MAbs with neutralizing activity against HBs antigens have been developed to amplify the effectiveness of NA therapy. Targeting NTCP can provide an effective treatment option for patients with chronic hepatitis B, regardless of viral genotype, mutations, and the presence of subviral particles.
JOURNAL OF VIROLOGY
(2022)
Review
Immunology
Yoshiko Matsuda, Takeshi Watanabe, Xiao-Kang Li
Summary: Controlling antibody-mediated allograft rejection requires prediction of onset, therapeutic intervention, and detailed immune monitoring methods. Research focuses on understanding the role of immune cells in LLPC differentiation, exploring novel control methods to enhance clinical applications and improve patient prognosis.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biology
Attila Ozsvar, Gergely Komlosi, Gaspar Olah, Judith Baka, Gabor Molnar, Gabor Tamas
Summary: Ionotropic receptor-mediated responses are critical in neuronal computation, while the arithmetic of summation for metabotropic signals is unclear. Experiments on neocortical neurogliaform cells revealed sublinear summation of ionotropic GABA(A) responses and linear summation of metabotropic GABA(B) responses. Model predictions suggest that one or two NGFCs can provide input to a point in the neuropil in 83% of cases and metabotropic GABAergic responses remain linear even with simultaneous activation of superficial layer interneurons specialized to recruit GABA(B) receptors.
Article
Immunology
Michela Ciocca, Salvatore Zaffina, Ane Fernandez Salinas, Chiara Bocci, Patrizia Palomba, Maria Giulia Conti, Sara Terreri, Giuseppe Frisullo, Ezio Giorda, Marco Scarsella, Rita Brugaletta, Maria Rosaria Vinci, Nicola Magnavita, Rita Carsetti, Eva Piano Mortari
Summary: The decline of the immune system in aging leads to decreased susceptibility to infections and reduced response to vaccination, especially in reacting to new pathogens. Research shows that elderly individuals have a significant reduction in CD27(dull) memory B cells, while having a higher population of specialized antigen-selected CD27(bright) memory B cells. Additionally, B cells from older individuals produce significantly fewer IgM and IgA antibodies compared to younger individuals after in vitro stimulation.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Laia Sadeghi, Anthony P. H. Wright
Summary: Multiple signaling pathways regulate the migration and adhesion of malignant B-cells, including the dysregulated NF-?B pathways in mantle cell lymphoma (MCL). Using a co-culture model system, it was found that KDM6B histone demethylase is associated with MCL cell adhesion to stromal cells. Inhibition of KDM6B reduces MCL adhesion and impairs the NF-?B pathway, suggesting KDM6B as a potential therapeutic target for MCL.
Article
Hematology
Helal Mohammed Mohammed Ahmed, Subbaiah Chary Nimmagadda, Yahya S. Al-Matary, Maren Fiori, Tobias May, Daria Frank, Pradeep Kumar Patnana, Christian Recher, Christoph Schliemann, Jan-Henrik Mikesch, Thorsten Koenig, Frank Rosenbauer, Wolfgang Hartmann, Jan Tuckermann, Ulrich Duhrsen, Wei Lanying, Martin Dugas, Bertram Opalka, Georg Lenz, Cyrus Khandanpour
Summary: This study showed functional differences between AML MSCs and HD MSCs, with Notch signaling in AML MSCs enhancing AML cell proliferation, while dexamethasone or Notch inhibitors could disrupt the interaction between AML cells and AML MSCs, improving treatment outcomes in AML patients.
BRITISH JOURNAL OF HAEMATOLOGY
(2022)
Article
Microbiology
Prasad Dasari, Maria Nordengruen, Claudia Vilhena, Leif Steil, Goran Abdurrahman, Kristin Surmann, Vishnu Dhople, Julia Lahrberg, Claus Bachert, Christine Skerka, Uwe Voelker, Barbara M. Broeker, Peter F. Zipfel
Summary: SplB, a protease produced by Staphylococcus aureus, inhibits the host immune response by cleaving specific human complement proteins. It reduces opsonophagocytosis by neutrophils and prevents deposition of C5b-9 on the bacterial surface. SplB is considered a novel staphylococcal complement evasion protein.
JOURNAL OF BACTERIOLOGY
(2022)
Article
Medicine, General & Internal
Johannes Forster, Andrea Streng, Paul Rudolph, Viktoria Ruecker, Julia Wallstabe, Sandra Timme, Franziska Pietsch, Katrin Hartmann, Maike Krauthausen, Julia Schmidt, Timo Ludwig, David Gierszewski, Thomas Jans, Geraldine Engels, Benedikt Weissbrich, Marcel Romanos, Lars Doelken, Peter Heuschmann, Christoph Haertel, Ildiko Gagyor, Marc Thilo Figge, Oliver Kurzai, Johannes Liese
Summary: The study conducted surveillance for SARS- CoV-2 in 9 German day care centers was feasible and well accepted. Mathematical modeling estimated that testing can minimize the spread of SARS-CoV-2 in day care centers.
Article
Engineering, Biomedical
T. N. M. Hoang, Z. Cseresnyes, S. Hartung, M. Blickensdorf, C. Saffer, K. Rennert, A. S. Mosig, M. von Lilienfeld-Toal, M. T. Figge
Summary: Invasive pulmonary aspergillosis is a serious infection with a high mortality rate. The invasive aspergillosis-on-chip (IAC) model allows the study of fungal growth and invasion in vitro. The model uses an algorithm-based analysis pipeline to visualize and quantify the morphology of the fungus. Macrophages in the IAC model inhibit fungal growth partially and contribute to the release of proinflammatory cytokines and chemokines. The fungistatic drug caspofungin limits fungal growth and causes morphological changes in the hyphal tree.
Article
Biotechnology & Applied Microbiology
Katherine Gonzalez, Gauri Gangapurwala, Julien Alex, Antje Vollrath, Zoltan Cseresnyes, Christine Weber, Justyna A. Czaplewska, Stephanie Hoeppener, Carl-Magnus Svensson, Thomas Orasch, Thorsten Heinekamp, Carlos Guerrero-Sanchez, Marc Thilo Figge, Ulrich S. Schubert, Axel A. Brakhage
Summary: The study investigated whether polymeric particles (PPs) can reach Aspergillus fumigatus conidia-containing phagolysosomes in macrophages. The results showed that PPs were efficiently taken up by macrophages and co-localized with conidia in the same phagolysosomes. Fusion of phagolysosomes containing PPs with phagolysosomes containing conidia was observed. This suggests that PPs can be used as a carrier system to target intracellular pathogens.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Urology & Nephrology
Maria de las Mercedes Noriega, Faeq Husain-Syed, Sonia Wulf, Benjamin F. Csala, Christian J. Krebs, Wolfram F. Jabs, Peter Zipfel, Hermann-Josef Groene, Thorsten Wiech
Summary: A study found a temporal association between SARS-CoV-2 vaccination and kidney diseases, as well as between COVID-19 and kidney diseases. The study compared kidney biopsies before and after vaccination, and found that glomerulopathies were more common in vaccinated patients, while tubulointerstitial diseases were more common in COVID-19 patients. The frequency of certain kidney diseases did not increase between 2019 and 2021.
CLINICAL JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2023)
Letter
Biochemical Research Methods
Ruman Gerst, Zoltan Cseresnyes, Marc Thilo Figge
Article
Microbiology
Marie Goldmann, Franziska Schmidt, Zoltan Cseresnyes, Thomas Orasch, Susanne Jahreis, Susann Hartung, Marc Thilo Figge, Marie von Lilienfeld-Toal, Thorsten Heinekamp, Axel A. Brakhage
Summary: Alveolar macrophages are the first line of defense against the inhaled conidia of Aspergillus fumigatus. The conidia's pigment interferes with the formation of lipid rafts, preventing the formation of functional phagolysosomes. Stomatin, an integral membrane protein, plays a role in the recruitment of the beta-glucan receptor dectin-1 and in phagosomal maturation.
Article
Engineering, Biomedical
WanLing Foo, Zoltan Cseresnyes, Carsten Roessel, Yingfeng Teng, Anuradha Ramoji, Mingzhe Chi, Walter Hauswald, Sophie Huschke, Stephanie Hoeppener, Juergen Popp, Felix H. Schacher, Marek Sierka, Marc Thilo Figge, Adrian T. Press, Michael Bauer
Summary: Targeting delivery of oligonucleotides or small molecular drugs to hepatocytes is challenging due to the efficient mononuclear phagocyte system of the liver. In this study, polyplex micelles were used as nanocarriers for drug delivery. By altering the ratio of poly(ethylene glycol) corona to siRNA complexed poly(allyl glycidyl ether) core, the micelles were able to overcome the macrophage-rich reticuloendothelial barrier and specifically target hepatocytes.
Article
Multidisciplinary Sciences
Fengjiao Ma, Rainer Kaufmann, Jaroslaw Sedzicki, Zoltan Cseresnyes, Christoph Dehio, Stephanie Hoeppener, Marc Thilo Figge, Rainer Heintzmann
Summary: Correlative light and electron microscopy combines the benefits of both techniques to study cell structure. However, the current method of overlaying light microscopy onto electron microscopy is limited due to differences in structural detail. This paper introduces a new approach called EM-guided deconvolution to bridge the resolution and specificity gaps. The method was tested on simulations, multi-color beads, and biological samples.
Article
Immunology
Claudia Vilhena, Shanshan Du, Miriana Battista, Martin Westermann, Thomas Kohler, Sven Hammerschmidt, Peter F. Zipfel
Summary: This study focused on the impact of three choline-binding proteins (CBPs), PspC, PspA, and LytA, on the adhesion and pathogenicity of Streptococcus pneumoniae. By using electron microscopy, confocal laser scanning microscopy, and functional quantitative assays, we found that LytA plays a crucial role in biofilm synthesis, while PspA and PspC are important for the hemolytic effects of S. pneumoniae on human red blood cells. Furthermore, all knockout mutants caused less damage to endothelial cells, highlighting the significance of each CBP for the overall pathogenicity of S. pneumoniae.
INFECTION AND IMMUNITY
(2023)
Meeting Abstract
Dermatology
S. Grau, S. Hartung, Z. Cseresnyes, M. Hoang, K. Renner, A. S. Mosig, M. T. Figge, M. Von Lilienfeld-Toal, S. Jahreis
Meeting Abstract
Dermatology
T. Orasch, G. Gangapurwala, K. Gonzalez, A. De San Luis, J. Alex, A. Vollrath, Z. Cseresnyes, C. Weber, S. Hoeppener, M. T. Figge, U. S. Schubert, A. A. Brakhage
Meeting Abstract
Dermatology
M. Rafiq, L. J. Jia, Z. Cseresnyes, M. Thilo Figge, A. A. Brakhage
Article
Materials Science, Biomaterials
Li-Yu Chen, Uwe Schirmer, Miriam Widder, Yves Gruel, Jerome Rollin, Peter F. Zipfel, Thi-Huong Nguyen
Summary: Heparin-induced thrombocytopenia (HIT) is a disease caused by newly formed platelet-activating antibodies. This study developed a cell-based ELISA method to improve the detection of HIT antibodies.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
Claudia Vilhena, Shanshan Du, Zoltan Cseresnyes, Lioba Zimmermann, Miriana Battista, Aurelie Jost, Christian Eggeling, Thomas Kohler, Christine Skerka, Sven Hammerschmidt, Marc Thilo Figge, Peter Zipfel
MOLECULAR IMMUNOLOGY
(2022)