Article
Nanoscience & Nanotechnology
Songbai Zhang, Yunxia Luan, Mengyi Xiong, Jingjing Zhang, Ryan Lake, Yi Lu
Summary: In this study, an aptamer-based sensing platform incorporating magnetic beads and a DNAzyme for signal amplification was developed for the detection of small-molecular targets, showing high sensitivity and good selectivity. By conjugating biotin-labeled aptamer probe and substrate strand on magnetic beads, and hybridizing DNAzyme strand to block substrate cleavage, specific binding of small-molecular targets can be detected and quantified effectively through an iterative signal amplification reaction. This platform enables the detection of toxins such as ochratoxin with a wide linear detection range, low detection limit, and the potential for portable, at-home analysis of small molecules using a glucose meter.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Analytical
Xiujin Chen, Dong Gao, Jiaqi Chen, Xueqing Wang, Chifang Peng, Hongli Gao, Yao Wang, Zhaozhou Li, Huawei Niu
Summary: In this study, an electrochemical aptasensor using PAMAM dendrimers as a signal amplifier was developed for the sensitive detection of OTA. The aptasensor showed high sensitivity, specificity, and a broad linear detection range with a low detection limit. The proposed aptasensor was successfully applied for OTA analysis in red wine and demonstrated good recovery rates and storage stability.
Article
Food Science & Technology
Chengyuan Wu, Xinjie Wang, Ling Guo, Xingxu Huang, Lina Wu, He Huang
Summary: A neoteric electrochemical biosensor based on an exonuclease III (Exo III)-assisted recycling reaction triggering the trans-cleavage ability of CRISPR-Cas12a was developed for the selective and ultrasensitive detection of ochratoxin A (OTA). The method showed good selectivity and sensitivity for OTA determination with a detection limit of 0.74 fg/mL. Furthermore, the established method could sensitively identify OTA in spiked rice and peanut samples, demonstrating its application potential for practical detection.
Article
Food Science & Technology
Yujiao Hou, Nan Long, Boyu Jia, Xiaofang Liao, Minghan Yang, Lizhu Fu, Lidong Zhou, Ping Sheng, Weijun Kong
Summary: This study developed a conveniently fabricated label-free electrochemical aptasensor for ultrasensitive detection of ochratoxin A (OTA) in food. The aptasensor showed high sensitivity and specificity, with a linear relationship between resistance and OTA concentration. The sensor exhibited promising practical applications in food control for identifying hazardous contaminants.
Article
Biochemistry & Molecular Biology
Yujiao Hou, Qingbin Xu, Ying Li, Nan Long, Peng Li, Jiabo Wang, Lidong Zhou, Ping Sheng, Weijun Kong
Summary: An ultrasensitive electrochemical aptasensor was developed using Nafion-stabilized functionalized multi-walled carbon nanotubes (f-MWCNTs) as signal enhancers for the determination of ochratoxin A (OTA). The f-MWCNTs were prepared through functionalization with nitric acid, and the incorporation of Nafion improved their dispersion and prevented leaching. The aptasensor showed a robust stability, and the Nafion-f-MWCNTs composites significantly amplified the electroactive surface area and conductivity, enabling selective recognition of OTA. The aptasensor exhibited a good linear relationship between the current changes and the logarithm of OTA concentration, with a low limit of detection of 1 pg/mL for OTA.
BIOELECTROCHEMISTRY
(2023)
Review
Chemistry, Analytical
Xiaoqian Tang, Jiasi Zuo, Cheng Yang, Jun Jiang, Qi Zhang, Jianfeng Ping, Peiwu Li
Summary: Biotoxins, including mycotoxins, marine toxins, and bacterial food toxins, pose significant threats in various fields such as food, feed, seafood, and medicine due to their potential harmful effects on humans. The increased consumption of fast food and prepared food without considering associated toxins is a critical issue. Therefore, the development of effective methods for monitoring biotoxins is crucial. In recent years, biosensors have been widely used for biotoxin detection due to their simplicity, quick detection procedure, low cost, and other advantages. This study provides a comprehensive overview of the application of biosensors in biotoxin detection, focusing on optical, electrochemical, piezoelectric, and photothermal applications. Additionally, the integration of different detection platforms as multimodal combination biosensors is discussed to meet the demand for highly accurate technologies. Major challenges faced by biosensors are examined, and novel insights for improving food safety technology are provided.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Yang Hu, Hanyin Xie, Jiaying Hu, Danting Yang
Summary: This study developed a novel biosensor based on disposable screen-printed carbon electrodes (SPCEs) for the ultrasensitive detection of ochratoxin A (OTA). Graphene oxide-DNA (GO-DNA) complex as a signal amplifier was investigated for the first time. The proposed aptasensor showed a low limit of detection and good reproducibility, and the OTA detection result was comparable to that of the ELISA kit.
Article
Chemistry, Applied
Yujiao Hou, Nan Long, Qingbin Xu, Ying Li, Pengyue Song, Minghan Yang, Jiabo Wang, Lidong Zhou, Ping Sheng, Weijun Kong
Summary: In this study, a label-free electrochemical aptasensor based on multi-walled carbon nanotubes (MWCNTs) and gold nanoparticles (Au NPs) was developed for ultrasensitive detection of ochratoxin A (OTA) in foods. The aptasensor exhibited excellent sensitivity with a detection limit as low as 1 pg/mL and a wide linear range of 1 pg/mL-10 ng/mL for OTA. It was also successfully applied to detect OTA in spiked malt samples and actual malt samples collected from the local market.
Article
Food Science & Technology
Wei Guo, Haoyu Yang, Yunzhe Zhang, Hao Wu, Xin Lu, Jianxin Tan, Wei Zhang
Summary: An ultrasensitive and labeled-free fluorescent aptamer sensor combining real-time fluorescence with strand displacement amplification (SDA) was developed for the determination of ochratoxin A in food. The sensor showed good linearity and a low limit of detection, as well as excellent specificity, reproducibility, and analytical performance in labeled detection of wheat and oat samples.
Article
Biochemical Research Methods
Jingfang Guo, Guodong Wang, Jing Zou, Zhen Lei
Summary: The peroxidase-like activity of two-dimensional titanium carbon MXene nanosheets (Ti3C2 NSs) was investigated, and it was found that Ti3C2 NSs could catalyze the oxidation of o-phenylenediamine (OPD) into 2,3-diaminophenazine (DAP) in the presence of H2O2. The catalytic behavior followed Michaelis-Menten kinetics and was attributed to nanozyme-accelerated electron transfer and generation of active species. Based on the controllable peroxidase-mimicking activity of Ti3C2 NSs, a label-free colorimetric aptasensor was developed for the sensitive detection of microcystin-LR (MC-LR) with good performance in real water samples.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biophysics
Xiaobo Zhang, Hui Zhi, Mingzhen Zhu, Fengya Wang, Hu Meng, Liang Feng
Summary: This study developed a novel paper-based analytical device for detecting Ochratoxin A, achieving dual-mode detection using a synthesized functional material to greatly enhance sensitivity. The device integrates visual and electrochemical signal output, providing a cost-effective and practical solution for resource-limited regions, and can be effectively applied in the field of food safety.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Applied
Qiulan Li, Qinqin Han, Dezhi Yang, Kexiang Li, Yijie Wang, Dan Chen, Yaling Yang, Hong Li
Summary: In this study, gold nanoparticles with weak peroxidase-like (POD) activity were synthesized using sulfur doped carbon dots as reducing agent and stabilizer. It was found that methylmercury could selectively regulate the POD-like activity of the gold nanoparticles, and ochratoxin A could simultaneously turn off the UV-vis absorbance signals and the surface-enhanced Raman scattering (SERS) signal. Based on these findings, a selective colorimetric-SERS dual-mode OTA detection strategy was established with gold amalgamation as the probe, achieving low limit of detection and good recoveries.
Article
Chemistry, Analytical
Xiujuan Qiao, Xin Ma, Xiaoyu Ma, Tianli Yue, Qinglin Sheng
Summary: The research showed that an ultrasensitive label-free aptasensor for ochratoxin A (OTA) detection was developed based on 2D MOF nanosheets, showing excellent performance with low detection limit, wide linear range, and stability.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Lixia Su, Yonghuan Su, Bingqian Liu
Summary: A new strategy for immobilization-free detection of E. coli was developed using a composite as capture probes and signal amplification labels, achieving target detection through a specific sandwich format. This method showed linear detection of E. coli concentration with a low limit of detection.
ANALYTICAL METHODS
(2022)
Article
Biophysics
Yahui Wang, Wei Song, Haiyan Zhao, Xin Ma, Shuying Yang, Xiujuan Qiao, Qinglin Sheng, Tianli Yue
Summary: In this study, an aptasensor for detecting Ochratoxin A was successfully constructed using gold nanoparticles decorated molybdenum oxide nanocomposites, hybridization chain reaction, and a restriction endonuclease-aided walker DNA machine. The sensor demonstrated excellent analytical performance and high sensitivity, with a detection limit as low as 3.3 fg mL-1, showing great potential for practical applications in various sample types.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Food Science & Technology
Ewelina Bigoraj, Iwona Kozyra, Agnieszka Kaupke, Zbigniew Osinski, James Lowther, Artur Rzezutka
Summary: The aim of this study was to assess the prevalence of human enteric viruses in mussels sold on the Polish market and study the fecal contamination of shellfish. The study found that human enteric viruses were commonly present in shellfish, with norovirus being the most frequently detected. The study also observed a seasonal distribution pattern of viruses in shellfish samples.
Editorial Material
Food Science & Technology
Bojan Blagojevic, Lis Alban Denmark
Article
Food Science & Technology
Indy Magnus, Fatemeh Abbasi, Hugo Thienpont, Lien Smeesters
Summary: Food safety and quality are increasingly important in our society, driving the development of novel optical food sensing technologies. However, current optical sensing technologies are often limited in their sensitivity and product variability. In this study, we developed a novel pistachio nut screening methodology using fluorescence spectroscopy and advanced chemometrics to simultaneously detect shells, tree parts, and aflatoxins. By optimizing hardware and software parameters, we achieved a high classification accuracy for healthy pistachio kernels.
Article
Food Science & Technology
Xiaodong Wang, Jingqiang Yu, Haixia Wu, Leiying Wu, Wuyunga Borjihan, Cong Wang, Munkhjargal Burenjargal, Alideertu Dong
Summary: This study developed a novel photon-phonon coupling (PPC) cold sterilization technology and compared its effects with low-temperature pasteurization and microwave ultraviolet. The results showed that PPC effectively reduced bacterial count in raw milk with minimal impact on milk quality and nutritional composition.
Review
Food Science & Technology
Riyadh Abdulmalek Hassan, Sharina Abu Hanifah, Lee Yook Heng, Fawaz Al-badaii, Alizar Ulianas
Summary: This study used bibliometric visualisation map analysis to explore the publication outputs, trends, and hotspots for endocrine disruptors (EDMs) biosensor development in relation to mycotoxins. The analysis showed that the current literature primarily focuses on developing biosensors for detecting highly toxic and widely distributed mycotoxins in food, but there needs to be more focus on biosensors for detecting mycotoxins known as endocrine disruptors.
Article
Food Science & Technology
Yajie Zheng, Jianpeng Dou, Changpo Sun, Hujun Liu, Shipei Zhang, Wenfu Wu
Summary: This study investigated the efficiency of modified SA/ll-glucan/Mt microsphere adsorbents in removing DON and ZEN from CSL. The microspheres showed high adsorption capacity, and simple and rapid regeneration was achieved without affecting the removal efficiency of mycotoxins from CSL.
Article
Food Science & Technology
Carlos Adelantado, Jose Angel Salatti-Dorado, Carlos Herrera, Lourdes Arce, Maria Jose Cardador
Summary: High-performance-ion mobility spectrometry (HPIMS) was used for the first time in the analysis of olive oil samples, following a simple liquid-liquid extraction procedure. The developed methodology is user-friendly, fast, and inexpensive, complying with the principles of green chemistry. The proposed approach, combined with multivariate analysis, showed potential in classifying olive oil samples based on their organoleptic quality, with high accuracy rates.
Article
Food Science & Technology
Jia-Wen Wei, Jia-Rong He, Shi-Yi Chen, Yi-Han Guo, Xuan-Zhu Huo, Nuan Zheng, Shuo-Hui Cao, Yao-Qun Li
Summary: Polycyclic aromatic hydrocarbons (PAHs) are a major cause of human cancer. This study developed a quantitative analysis method for PAH4 using the back propagation neural network (BPNN) algorithm and constant wavelength synchronous fluorescence (CWSF) spectra as the data sets. The method can predict the concentrations of PAH4 in edible oil samples without preprocessing or pre-separation. It has been proven to be a powerful tool for the rapid detection of PAH4.
Article
Food Science & Technology
Liyun Chen, Xiumei Li, Jingyi Chen, Ranxun Lin, Yuhan Mai, Yuxin Lin, Guodong Wang, Zheng Chen, Wei Zhang, Jiang Wang, Cai Yuan, Longguang Jiang, Peng Xu, Mingdong Huang
Summary: Food spoilage is primarily caused by microbial contamination. Curcumin has shown moderate antimicrobial activity as a food preservative. This study formulates curcumin with zinc acetate to enhance its efficacy, resulting in Cur-Zn with increased antimicrobial activity against multiple food-contaminating bacteria and fungi. With light illumination, the antimicrobial activity of Cur-Zn is further enhanced due to the photodynamic inactivation (PDI) effect.
Article
Food Science & Technology
Marta Pilar Ortiz-Moriano, Gonzalo Machado-Schiaffino, Eva Garcia-Vazquez, Alba Ardura
Summary: Mislabelling in the global fisheries sector raises concerns about the identity, safety, and sustainability of seafood products. This study examined mislabelling in shrimps and prawns sold in the Spanish market and assessed potential risks of heavy metal ingestion.
Article
Food Science & Technology
Doyeon Kim, Jinwoo Kim, Minsik Kim
Summary: This study demonstrates the enhanced bactericidal activity of engineered lysins fused with amphipathic antimicrobial peptides against Gram-negative bacterium Cronobacter sakazakii. The fusion lysins are capable of disrupting bacterial cells and significantly reducing the risk of infection within a certain range of temperature and pH. Additionally, the study also shows the bactericidal effect of these fusion lysins against other Gram-negative pathogens.
Article
Food Science & Technology
Seul-Ki Park, Kyung-Jin Cho, Du-Min Jo, Young-Mog Kim
Summary: This study presents a new approach for detecting pathogenic Vibrio spp. in contaminated seafood, which combines PCR with a simplified DNA extraction process and pre-culturing step. The method has been validated and shown to be efficient, time-saving, and highly sensitive, offering a potential alternative for improving safety measures in the food industry.
Article
Food Science & Technology
Suwimol Surareungchai, Chaleeda Borompichaichartkul, Chitsiri Rachtanapun, Nutthachai Pongprasert, Pongphen Jitareerat, Varit Srilaong
Summary: This study builds upon previous research and focuses on risk assessment and analysis in the organic salad industry. The results show that previous risk matrix models can be adapted for various raw materials, and preventive control plans can be applied across different aspects of the production process.
Article
Food Science & Technology
Xuan Zhang, Changling Fang, Xiaoyi Lou, Feng Han, Siman Li, Yongfu Shi, Dongmei Huang
Summary: This study aimed to assess the levels of Biogenic amines (BAs) in four common food types in the Chinese market. It was found that cheese and seafood derived product showed higher levels of BAs, and the histamine level in mackerel and yellow rice wine exceeded the limits.
Article
Food Science & Technology
Martina Foschi, Alessandra Biancolillo, Federico Marini, Francesco Cosentino, Francesca Di Donato, Angelo Antonio D'Archivio
Summary: This study aimed to develop a potentially non-destructive and routine-compatible method for the geographical traceability of high-valued Italian chickpeas. By analyzing the spectral information of the outer and inner parts of the kernels, it was found that the spectral analysis of the outer part yielded accurate and reliable results, indicating the possibility of non-destructive authentication.