Article
Agriculture, Dairy & Animal Science
Umberto Molini, Giovanni Franzo, Tirumala B. K. Settypalli, Maria Y. Hemberger, Siegfried Khaiseb, Giovanni Cattoli, William G. Dundon, Charles E. Lamien
Summary: Important swine pathogens, African swine fever virus (ASFV) and porcine parvovirus 1 (PPV1), were identified in warthogs in Namibia and genetically characterized in this study. The findings will be of interest to those working in swine disease management and control. Understanding virus circulation in wild animals, especially those in contact with domestic animals, is crucial for disease management and control.
Article
Immunology
Wen Zhang, Zhendong Fu, Hongyan Yin, Qingbing Han, Wenhui Fan, Fangkun Wang, Yingli Shang
Summary: PCV2 infection skews macrophages towards M1 status by reprogramming the expression of M1 and M2 associated genes. This skewing is regulated by activation of NF-κB and JNK signaling pathways for M1-associated genes and suppression of M2-associated genes through inhibition of JMJD3 expression. This modulation by PCV2 contributes to bacterial coinfection and viral pathogenesis.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Microbiology
Fuyu Du, Zhi Cao, Zixuan Ye, Jun He, Weijie Zhang, Ke Zhang, Pengbo Ning
Summary: This study successfully constructed a recombinant viral plasmid vaccine for the prevention and control of Classical swine fever virus (CSFV) and porcine Circovirus type 2 (PCV2), and showed its immune efficacy.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Veterinary Sciences
Heinner F. Uribe-Garcia, Rafael A. Suarez-Mesa, Iang S. Rondon-Barragan
Summary: In this study, the prevalence of porcine circovirus 2 (PCV2) and porcine parvovirus (PVP) in the department of Tolima, Colombia, was investigated. The results showed that PCV2d was detected in 90% of samples, while PVP was found in 2.6% of samples. Furthermore, genetic variations in the PCV2 capsid gene were identified, which may be associated with virus pathogenicity.
VETERINARY MEDICINE AND SCIENCE
(2022)
Article
Immunology
Qi Gao, Yunlong Yang, Yizhuo Luo, Jiachen Zheng, Lang Gong, Heng Wang, Yongzhi Feng, Ting Gong, Dongdong Wu, Ruixia Wu, Xiaoyu Zheng, Zezhong Zheng, Guihong Zhang
Summary: African swine fever virus (ASFV) is a significant threat to the global swine industry, and its infection relies on the receptors CD163 and Siglec1 on host cells, while inhibiting the host immune response.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Dipak Deka, Nagendra Nath Barman, Nipu Deka, Balwinder Kaur Batth, Gagandeep Singh, Sarabjot Singh, Ravi Kant Agrawal, Chandra Sekhar Mukhopadhyay, Ramneek
Summary: Reproductive problems in swine caused by porcine viruses pose a serious threat to the pig industry in developing countries like India. A study conducted in different regions of India revealed high seroprevalence of classical swine fever virus, porcine circovirus-2, and porcine parvovirus. The research indicated that single infections were more prevalent than co-infections, providing important insights for the prevention and control of these viruses.
TROPICAL ANIMAL HEALTH AND PRODUCTION
(2021)
Article
Biochemistry & Molecular Biology
Xiangqi Hao, Yanchao Li, Hui Chen, Bo Chen, Ruohan Liu, Yidan Wu, Xiangyu Xiao, Pei Zhou, Shoujun Li
Summary: Canine circovirus (CanineCV) is an emerging virus in canines that is becoming a global epidemic. This study aimed to address the lack of knowledge about CanineCV by successfully isolating and characterizing the virus. The researchers were able to rescue a CanineCV strain in cell culture and found that the viral Rep protein is associated with cytopathic effects and immune suppression. These findings have important implications for understanding CanineCV infection and its interaction with other pathogens.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Infectious Diseases
Melina Fischer, Jutta Pikalo, Martin Beer, Sandra Blome
Summary: The study found that storing contaminated SDPP at room temperature for at least 2 weeks can significantly reduce the risk of ASFV transmission. In contrast, heavily contaminated SDPP stored at 4 degrees Celsius may remain infectious for at least 5 weeks.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Article
Virology
Aruna Ambagala, Kalhari Goonewardene, Lindsey Lamboo, Melissa Goolia, Cassidy Erdelyan, Mathew Fisher, Katherine Handel, Oliver Lung, Sandra Blome, Jacqueline King, Jan Hendrik Forth, Sten Calvelage, Edward Spinard, Douglas P. Gladue, Charles Masembe, Adeyinka J. Adedeji, Toyin Olubade, Nanven A. Maurice, Hussaini G. Ularamu, Pam D. Luka
Summary: African swine fever (ASF), a highly contagious hemorrhagic fever of pigs, poses a threat to global socio-economic issues, food security, and biodiversity. Nigeria experienced a major outbreak of ASF in 2020, resulting in the death of nearly half a million pigs. Genetic analysis identified the virus responsible for the outbreak as African swine fever virus (ASFV) p72 genotype II. Further characterization of ASFV RV502, one of the isolates from the outbreak, revealed a deletion and reverse complement duplication in its genome. Phylogenetic analysis suggests that the virus originated from Southeastern Africa.
Article
Agriculture, Multidisciplinary
Song Xiang-peng, Xia Ying-ju, Xu Lu, Zhao Jun-jie, Wang Zhen, Zhao Qi-zu, Liu Ye-bing, Zhang Qian-yi, Wang Qin
Summary: With the implementation of the C-strain vaccine, classical swine fever (CSF) has been controlled in China, while African swine fever (ASF) and atypical porcine pestivirus (APPV) still pose challenges to the pig industry. It is crucial to develop a reliable method for simultaneous detection and differential diagnosis of these diseases. In this study, a multiplex real-time PCR assay was established, which showed high sensitivity and specificity for the detection of CSFV, ASFV, and APPV.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2023)
Article
Biotechnology & Applied Microbiology
S. Rajkhowa, M. Choudhury, S. R. Pegu, D. K. Sarma, V. K. Gupta
Summary: A novel one-step triplex PCR assay was developed for simultaneous detection of three important viruses involved in reproductive problems in pigs. The assay showed high specificity and sensitivity, making it a useful tool for clinical diagnosis of mixed infections of DNA and RNA viruses in pigs.
LETTERS IN APPLIED MICROBIOLOGY
(2022)
Article
Infectious Diseases
Adeyinka J. Adedeji, Rebecca B. Atai, Helen E. Gyang, Panzam Gambo, Maimuna A. Habib, Rebecca Weka, Vincent B. Muwanika, Charles Masembe, Pam D. Luka
Summary: Livestock trading through live animal markets, such as the designated live pig markets in Nigeria, can introduce and spread economically important pathogens like the African swine fever virus (ASFV). This study analyzed data and pig samples from selected live pig markets in Nigeria and found that these markets were hotspots and channels for the transmission and spread of ASFV in the country. Disease surveillance and regulation at these markets are critical for controlling ASF in Nigeria.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Biotechnology & Applied Microbiology
Dongjie Chen, Di Wang, Caixia Wang, Fang Wei, Hongyuan Zhao, Xiangmei Lin, Shaoqiang Wu
Summary: The study developed a rapid and highly sensitive ASFV antigen detection method based on AlphaLISA, which showed high specificity and outperformed other detection methods in porcine serum samples. The method serves as a sensitive and efficient laboratory diagnostic tool for ASFV detection.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Kentaro Masujin, Tomoya Kitamura, Ken Ichiro Kameyama, Kota Okadera, Tatsuya Nishi, Takato Takenouchi, Hiroshi Kitani, Takehiro Kokuho
Summary: The study showed that a novel cell line of immortalized porcine kidney macrophages (IPKM) has high levels of viral replication capability for African swine fever virus (ASFV), making it a valuable tool for ASF research.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Joshua A. Jackman, Erik Arabyan, Hovakim Zakaryan, Charles C. Elrod
Summary: Research shows that glycerol monolaurate (GML) can effectively inhibit wild-type ASFV infection of porcine macrophages, and the onset of antiviral activity corresponds to the critical micelle concentration of GML.
Article
Infectious Diseases
Giovanni Franzo, Tirumala B. K. Settypalli, Ebere Roseann Agusi, Clement Meseko, Germaine Minoungou, Bruno Lalidia Ouoba, Zerbo Lamouni Habibata, Abel Wade, Jose Luis de Barros, Cure Georges Tshilenge, Esayas Gelaye, Martha Yami, Danie Gizaw, Tesfaye Rufae Chibssa, Iolanda Vieira Anahory, Lourenco P. Mapaco, Sara J. Acha, Jeremiah Ijomanta, Anvou Rachael Jambol, Adeyinka Jeremy Adedeji, Pam Dachung Luka, David Shamaki, Mariame Diop, Mame Thierno Bakhoum, Modou Moustapha Lo, Jelly S. Chang'a, Bishop Magidanga, Charles Mayenga, Maureen Wakwamba Ziba, George Dautu, Charles Masembe, Jenna Achenbach, Umberto Molini, Giovanni Cattoli, Charles E. Lamien, William G. Dundon
Summary: The study investigated the presence of PCV-2 in domestic pigs in multiple African countries, identifying four genotypes circulating in the continent. Phylogeographic analysis revealed African-specific clusters and estimated the introduction time of PCV-2s into Africa, highlighting the significant implications for pig production on the continent.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Infectious Diseases
Adeyinka J. Adedeji, Rebecca B. Atai, Helen E. Gyang, Panzam Gambo, Maimuna A. Habib, Rebecca Weka, Vincent B. Muwanika, Charles Masembe, Pam D. Luka
Summary: Livestock trading through live animal markets, such as the designated live pig markets in Nigeria, can introduce and spread economically important pathogens like the African swine fever virus (ASFV). This study analyzed data and pig samples from selected live pig markets in Nigeria and found that these markets were hotspots and channels for the transmission and spread of ASFV in the country. Disease surveillance and regulation at these markets are critical for controlling ASF in Nigeria.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Immunology
Mabusetsa R. J. Makalo, William G. Dundon, Tirumala B. K. Settypalli, Sneha Datta, Charles E. Lamien, Giovanni Cattoli, Moeketsi S. Phalatsi, Relebohile J. Lepheana, Mpaliseng Matlali, Relebohile G. Mahloane, Marosi Molomo, Palesa C. Mphaka
Summary: In May 2021, Lesotho reported its first outbreak of highly pathogenic avian influenza (HPAI) caused by H5N1 subtype. The virus showed high genetic similarity with A/H5N1 viruses identified in Nigeria and Senegal. This discovery has significant implications for disease management and food security in the region.
EMERGING MICROBES & INFECTIONS
(2022)
Article
Microbiology
Janika Wolff, Eeva Tuppurainen, Adeyinka Adedeji, Clement Meseko, Olayinka Asala, Jolly Adole, Rebecca Atai, Banenat Dogonyaro, Anja Globig, Donata Hoffmann, Martin Beer, Bernd Hoffmann
Summary: Lumpy skin disease virus, a serious poxvirus that affects agricultural livestock, has a significant impact on the economy and global trade restrictions. This study confirmed the high pathogenicity of a Nigerian strain of the virus in cattle through experimental infection.
Article
Microbiology
Chukwunonso Onyilagha, Kelvin Nguyen, Pam D. Luka, Ularamu Hussaini, Adeyinka Adedeji, Theophilus Odoom, Aruna Ambagala
Summary: The study investigated the ability of a commercially available LFA test to detect African swine fever virus (ASFV) in clinical samples. The results showed that the LFA test was specific and able to detect ASFV in the early stages of infection. Although less sensitive than laboratory-based PCR assays, the LFA test has the potential to be a useful tool for on-site diagnosis in field settings.
Article
Virology
Giovanni Franzo, Habibata Lamouni Zerbo, Bruno Lalidia Ouoba, Adama Drabo Dji-Tombo, Marietou Guitti Kindo, Rasablaga Sawadogo, Jelly Chang'a, Stella Bitanyi, Aloyce Kamigwe, Charles Mayenga, Modou Moustapha Lo, Mbengue Ndiaye, Aminata Ba, Gaye Laye Diop, Iolanda Vieira Anahory, Lourenco P. Mapaco, Sara J. Acha, Valere Kouame Kouakou, Emmanuel Couacy-Hymann, Stephen G. Gacheru, Jacqueline K. Lichoti, Justus K. Kasivalu, Obadiah N. Njagi, Tirumala B. K. Settypalli, Giovanni Cattoli, Charles E. Lamien, Umberto Molini, William G. Dundon
Summary: Porcine parvovirus 1 (PPV1) is a major cause of reproductive failure in pigs, and the emergence of virulent strains and low vaccine protection is of increasing concern. In this study, 71 sequences from eight African countries were analyzed, revealing a pattern similar to that observed in high-income regions. The African scenario has been shaped by international movement of PPV1 from Europe and Asia, emphasizing the need for careful evaluation of control strategies.
Article
Immunology
Umberto Molini, John Yabe, Irene K. Meki, Hatem Ouled Ahmed Ben Ali, Tirumala B. K. Settypalli, Sneha Datta, Lauren Michelle Coetzee, Ellini Hamunyela, Siegfried Khaiseb, Giovanni Cattoli, Charles E. Lamien, William G. Dundon
Summary: In January 2022, a significant number of Cape cormorants in Namibia's west coast were found dead, and subsequent testing confirmed the presence of H5N1 avian influenza. Genomic and phylogenetic analysis revealed that the viruses belonged to clade 2.3.4.4b and were closely related to viruses identified in Lesotho and Botswana in 2021. This study provides the first genomic characterization of H5N1 viruses in Namibia and highlights the significance for poultry disease management and wildlife conservation in the region.
EMERGING MICROBES & INFECTIONS
(2023)
Article
Virology
David Oludare Omoniwa, Irene Kasindi Meki, Caleb Ayuba Kudi, Anthony Kojo Sackey, Maryam Aminu, Adeyinka Jeremy Adedeji, Clement Adebajo Meseko, Pam Dachung Luka, Olayinka Oluwafemi Asala, Jolly Amoche Adole, Rebecca Bitiyong Atai, Yakubu Joel Atuman, Tirumala Bharani Kumar Settypalli, Giovanni Cattoli, Charles Euloge Lamien
Summary: This study investigated suspected LSD outbreaks in cattle herds in Nigeria and found co-infection of LSDV and PCPV, highlighting the need for accurate diagnosis and control measures.
Article
Virology
Aruna Ambagala, Kalhari Goonewardene, Lindsey Lamboo, Melissa Goolia, Cassidy Erdelyan, Mathew Fisher, Katherine Handel, Oliver Lung, Sandra Blome, Jacqueline King, Jan Hendrik Forth, Sten Calvelage, Edward Spinard, Douglas P. Gladue, Charles Masembe, Adeyinka J. Adedeji, Toyin Olubade, Nanven A. Maurice, Hussaini G. Ularamu, Pam D. Luka
Summary: African swine fever (ASF), a highly contagious hemorrhagic fever of pigs, poses a threat to global socio-economic issues, food security, and biodiversity. Nigeria experienced a major outbreak of ASF in 2020, resulting in the death of nearly half a million pigs. Genetic analysis identified the virus responsible for the outbreak as African swine fever virus (ASFV) p72 genotype II. Further characterization of ASFV RV502, one of the isolates from the outbreak, revealed a deletion and reverse complement duplication in its genome. Phylogenetic analysis suggests that the virus originated from Southeastern Africa.
Article
Microbiology
Ulaankhuu Ankhanbaatar, Agathe Auer, Gerelmaa Ulziibat, Tirumala B. K. Settypalli, Delgerzul Gombo-Ochir, Ganzorig Basan, Taichiro Takemura, Erdene-Ochir Tseren-Ochir, Hatem Ouled Ahmed, Irene Kasindi Meki, Sneha Datta, Baba Soumare, Artem Metlin, Giovanni Cattoli, Charles E. Lamien
Summary: This study reports the whole-genome sequence of African swine fever virus (ASFV) detected in a Mongolian wild boar and compares it with other samples from Asia and Europe. The study finds differences between the Asian and European genome sequences and high similarity between ASFV variants in wild boars and domestic pigs, suggesting potential transmission through outdoor animal breeding.
Article
Veterinary Sciences
Adeyinka Adedeji, Paul Abdu, Olatunde Akanbi, Pam Luka
Summary: This study investigated the outbreaks of Marek's disease in poultry farms in Nigeria using PCR and histopathology. Despite vaccination, the disease still occurs and causes severe lesions in affected chickens.
VETERINARIA ITALIANA
(2022)
Article
Veterinary Sciences
Nancy Milton Sati, Pam Dachung Luka, Frank Norbert Mwiine, Idowu Oluwabunmi Fagbamila, Rebecca Paul Weka, Maryam Muhammad, Joseph Erume
Summary: This study assessed the perceptions and practices of indigenous poultry farmers towards Salmonella infections in Nigeria. The results revealed a poor level of perception among farmers and highlighted various associated practices. It is necessary to raise awareness about these infections to improve indigenous poultry health and public health.
OPEN VETERINARY JOURNAL
(2022)
Article
Veterinary Sciences
Adeyinka Jeremy Adedeji, Jolly Amoche Adole, Olayinka Oluwafemi Asala, Ahmed Abdulkadir Gamawa, Nanven Abraham Maurice, Anvou Jambol, Mohammed Bashir Bolajoko, Nneka Chineze Chima, Victoria Isioma Ifende, Yiltawe Simwal Wungak, Timothy Yusufu Woma, Pam Dachung Luka
Summary: This study provides the first nationwide epidemiological data on contagious ecthyma (CE) in sheep and goats in Nigeria. It also reports the molecular characterization of circulating ORFV causing outbreaks in these small ruminants. The study reveals that CE is under-reported, widespread, and economically important to sheep and goat farmers in Nigeria.
OPEN VETERINARY JOURNAL
(2022)
