4.7 Article

Kinetics of PAH degradation by a new acid-metal-tolerant Trabulsiella isolated from the MGP site soil and identification of its potential to fix nitrogen and solubilize phosphorous

期刊

JOURNAL OF HAZARDOUS MATERIALS
卷 307, 期 -, 页码 99-107

出版社

ELSEVIER SCIENCE BV
DOI: 10.1016/j.jhazmat.2015.12.068

关键词

Mixed contamination; HMW PAHs degradation; Diazotrophic bacteria; Phosphate solubilizer; Heavy metal resistance

资金

  1. Australian Government
  2. University of South Australia
  3. Cooperative Research Centre for Contamination Assessment and Remediation of the Environment (CRC CARE)

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Development of an efficient bioinoculum is considered as an appropriate remedial approach to treat the PAHs-metal mixed contaminated sites. Therefore, we aimed to isolate a degrader able to exert an outstanding PAH catabolic potential with added traits of pH-metal-resistance, N-fix or P-solubilization from a manufactured gas plant site soil. The identified strain (MTS-6) was a first low and high molecular weight (LMW and HMW) PAHs degrading Trabulsiella sp. tolerant to pH 5. MTS6 completely degraded the model 3 [150mgL(-1) phenanthrene (Phe)], 4 [150mgL(-1) pyrene (Pyr)] and 5 [50mgL(-1) benzo[a]pyrene (BaP)] ring PAHs in 6, 25 and 90 days, respectively. Presence of co-substrate (100 mgL(-1) Phe) increased the biodegradation rate constant (k) and decreased the halflife time (t(1/2)) of HMW PAHs (100mgL(-1) Pyr or 50mgL(-1) BaP). The strain fixed 47 mu gmL(-1) N and solubilized 58 mu gmL(-1) P during PAH metabolism and exhibited an EC50 value of 3-4mgL(-1) for Cu, Cd, Pb and Zn. Over 6mgL(-1) metal levels was lethal for the microbe. The identified bacterium (MTS-6) with exceptional multi-functional traits opens the way for its exploitation in the bioremediation of manufactured gas plant sites in a sustainable way by employing bioaugmentation strategy. (C) 2016 Elsevier B.V. All rights reserved.

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