Article
Chemistry, Multidisciplinary
Duncan K. Brownsey, Christopher J. Gafuik, Dae-Sun Kim, Leonie OSullivan, Evgueni Gorobets, Samuel Krukowski, Madison Turk, Craig N. Jenne, Douglas J. Mahoney, Darren J. Derksen
Summary: This study demonstrated that the fluorescence of pomalidomide can be used in high-throughput screening assays to rapidly assess the cellular penetration of degrader candidates. Additionally, this technique can provide insight into the potential mechanisms of candidates entering a target cell by using endocytosis inhibitors.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Duncan K. Brownsey, Christopher J. Gafuik, Dae-Sun Kim, Leonie OSullivan, Evgueni Gorobets, Samuel Krukowski, Madison Turk, Craig N. Jenne, Douglas J. Mahoney, Darren J. Derksen
Summary: This study demonstrates that the fluorescence of pomalidomide can be used to rapidly assess cellular penetration of degrader candidates in high-throughput screening assays, and can be combined with endocytosis inhibitors to gain insight into potential mechanisms of candidate entry into target cells.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Analytical
Weifeng Zhang, Yun Li, Lin Yao, Fan Wu, Chen Chen, Liqun Zhou, Bo Zheng, Pu Wang, Weili Hong
Summary: A new method based on SRS microscopy was reported to rapidly determine the antimicrobial susceptibility of beta-lactam antibiotics by measuring both the metabolic activity and morphological deformation of bacteria. The evaluation method achieved a 93.2% categorical agreement and a 93.2% essential agreement with the standard reference method when performing AST of cefotaxime on 103 E. coli strains.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Multidisciplinary
Kai Cui, Ruike Li, Yongming Zhang, Yuanyuan Qiu, Na Zhao, Yanna Cui, Wenwei Wu, Tize Liu, Zeyu Xiao
Summary: This study presents a molecular planarization strategy for adjusting the signal shift of Raman probes. By modifying the backbone of P3HT polymer, we obtained a PCPDT probe with a blueshifted characteristic signal. The PCPDT probe successfully avoids interference with lipid signals in tissue and enables high-precision intraoperative imaging.
Article
Microbiology
Giuseppe Pezzotti, Miyuki Kobara, Tamaki Nakaya, Hayata Imamura, Tenma Asai, Nao Miyamoto, Tetsuya Adachi, Toshiro Yamamoto, Narisato Kanamura, Eriko Ohgitani, Elia Marin, Wenliang Zhu, Ichiro Nishimura, Osam Mazda, Tetsuo Nakata, Koichi Makimura
Summary: This study used Raman spectroscopy and machine learning algorithms to investigate the susceptibility and metabolic reactions of Candida auris to antifungal drugs. The study found that AmB treatment induced biofilm formation in both clades, but the structures of the formed biofilms were different. Treatment with 5-FC caused yeast-to-hyphal or pseudo-hyphal morphogenesis in both clades. It was also observed that the South African Clade III demonstrated superior capacity in reducing membrane permeability to the drug.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Cell Biology
Yajuan Li, Wenxu Zhang, Anthony A. Fung, Lingyan Shi
Summary: This study presents a new optical imaging platform to directly visualize and quantify lipid metabolism in Drosophila fat body regulated by different diets and insulin signaling pathway. The findings reveal the interconnections between lipid metabolism, diets, and aging, providing a better understanding of these processes.
Article
Engineering, Environmental
Shuangxi Li, Liandong Zhu
Summary: Microalgae-fungi co-cultivation regulated by Cu(II) was investigated for nutrient and sulfonamides degradation in simulated swine wastewater. Results showed that Cu(II) enhanced the removal of nitrogen, phosphorus, and COD, as well as the degradation of sulfonamides through biodegradation. Mass spectrometry analysis revealed the degradation pathways of sulfonamides. Cu(II) induction of dissolved organic matters was found to reduce its concentration and mitigate organismal damage.
Review
Oncology
Lucas Becker, Nicole Janssen, Shannon L. Layland, Thomas E. Muerdter, Anne T. Nies, Katja Schenke-Layland, Julia Marzi
Summary: The hurdles of effective tumor therapy lie in delayed detection and limited effectiveness of systemic drug therapies due to patient-specific multidrug resistance. Non-invasive bioimaging tools like fluorescence lifetime imaging microscopy (FLIM) and Raman-microspectroscopy have evolved in the past decade, showing promise in early disease detection, drug screening, and personalized medicine studies.
Article
Chemistry, Multidisciplinary
Kosuke Dodo, Ayato Sato, Yuki Tamura, Syusuke Egoshi, Koichi Fujiwara, Kana Oonuma, Shuhei Nakao, Naoki Terayama, Mikiko Sodeoka
Summary: The study found that GLA itself exhibits tumor-selective cytotoxicity, independent of its oxidized metabolites. Deuteration of GLA can reduce toxicity towards normal cells and increase selectivity for tumor cells. Raman imaging confirmed the accumulation of fully deuterated GLA in intracellular lipid droplets of tumor cells.
CHEMICAL COMMUNICATIONS
(2021)
Review
Chemistry, Multidisciplinary
Sandra Cortijo-Campos, Rafael Ramirez-Jimenez, Alicia de Andres
Summary: This article discusses the current research status of novel platforms and metamaterials for enhancing optical and Raman signals, with a focus on plasmonic materials and graphene-related materials. The importance of reproducibility and homogeneity in quantifying probe molecules is emphasized. Additionally, the review highlights the potential of graphene in designing and fabricating novel architectures for light enhancement mechanisms.
Article
Multidisciplinary Sciences
Gabriel Perez-Gonzalez, Geoffrey A. Tompsett, Kyle Mastalerz, Michael T. Timko, Barry Goodell
Summary: Beta-glucan of ECM from fungi forms a hydrogel with oxalate at room temperature. The rheometric properties and protein diffusion of the hydrogel are influenced by oxalate concentration. This finding has implications for understanding wood-decay fungi and other pathogenic/biomedical fungi.
Article
Chemistry, Analytical
Brian T. Scarpitti, Chureeporn Chitchumroonchokchai, Steven K. Clinton, Zachary D. Schultz
Summary: The ability to monitor the uptake and distribution of food nutrients in in vitro cell culture models is vital for understanding the effectiveness of nutraceuticals in treating and preventing diseases. In this study, live-cell Raman microscopy was used to visualize the delivery of lycopene to prostate cancer cells and assess its cellular uptake. The results showed that lycopene likely accumulates in lipid membranes of cytoplasmic organelles. This label-free technique provides a convenient and sensitive method for detecting and quantifying lycopene uptake in living cells.
ANALYTICAL CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Maria Mangini, Maria Antonietta Ferrara, Gianluigi Zito, Stefano Manago, Alberto Luini, Anna Chiara De Luca, Giuseppe Coppola
Summary: Circulating tumor cells (CTCs), which retain tumor properties and heterogeneity, have high potential clinical values. However, the detection and characterization of CTCs face challenges due to their rarity and difficulty in distinguishing them from white blood cells. Efficient and rapid methods are needed to capture the broad spectrum of tumor cells circulating in the blood.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Dermatology
Syed Mehmood Ali
Summary: Skin aging is a dynamic process affected by intrinsic and extrinsic factors, with in vivo confocal Raman spectroscopy being a useful tool to detect AGE markers in old adult and diabetic male participants, accelerating the aging process.
PHOTODERMATOLOGY PHOTOIMMUNOLOGY & PHOTOMEDICINE
(2021)
Review
Biotechnology & Applied Microbiology
Priyanka Bokade, Hemant J. Purohit, Abhay Bajaj
Summary: Synthetic chemicals, including organochlorine pesticides, pose hazards to the environment and health due to their persistent and bioaccumulative properties. Fungi play a crucial role in biotransformation and degradation of toxic pollutants, with potential use of fungal-derived biomaterials for pesticide and heavy metal removal. Utilizing fungi for initial biotransformation purposes before bacterial degradation shows promise in overcoming existing bioremediation limitations.
INDIAN JOURNAL OF MICROBIOLOGY
(2021)
Article
Engineering, Biomedical
Jian He, Qiaolin Wei, Shoujie Wang, Shiyuan Hua, Min Zhou
Summary: The novel hollow AgAu-DTTC-BSA nanoprobes exhibit excellent biocompatibility and photothermal effects, allowing on-demand activation at tumor sites without harming normal cells. These nanoprobes can be used for tumor diagnosis and effectively treat colorectal solid neoplasms.
Article
Biotechnology & Applied Microbiology
Qiaolin Wei, Jian He, Shuaifei Wang, Shiyuan Hua, Yuchen Qi, Fangyuan Li, Daishun Ling, Min Zhou
Summary: The study developed a Raman-tagged hollow gold nanoparticle with SERS property, which efficiently accumulated in the tumor site under low-dose X-ray pre-irradiation and showed highly effective antitumor effects in a breast cancer model. It integrated Raman/PA imaging and PTT functions into a single platform, with promising prospects for clinical applications in enhancing drug delivery and accumulation in tumor theranostics, especially in combination with low-dose X-ray irradiation as an adjuvant.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Xiuhui Lin, Jian He, Wanlin Li, Yuchen Qi, Huiqun Hu, Dongxiao Zhang, Feng Xu, Xiaoyuan Chen, Min Zhou
Summary: The encapsulated lysostaphin microspheres (LyIR@MS) effectively treat MRSA-induced pneumonia by improving biofilm sensitivity to host neutrophils, enhancing survival rate, and reducing inflammatory injury.
Article
Multidisciplinary Sciences
Dongxiao Zhang, Danni Zhong, Jiang Ouyang, Jian He, Yuchen Qi, Wei Chen, Xingcai Zhang, Wei Tao, Min Zhou
Summary: In this study, researchers have successfully developed an oral delivery system using Spirulina platensis as a microcarrier to protect the whole small intestine from radiation-induced injury during radiotherapy. The system accumulates the drug effectively and provides superior radioprotection compared to free drug and enteric capsules, preventing radiation-induced intestinal injury without affecting tumor regression. It also has benefits on gut microbiota homeostasis and long-term safety.
NATURE COMMUNICATIONS
(2022)
Article
Nanoscience & Nanotechnology
Xiaopeng Cai, Shiyuan Hua, Jingwen Deng, Zhen Du, Dongxiao Zhang, Zhenfeng Liu, Nazif Ullah Khan, Min Zhou, Zhi Chen
Summary: Astaxanthin (ASX) can prevent APAP-induced liver injury (AILI) by activating the Nrf2/HO-1 pathway, and the ASX-loaded hollow mesoporous silica nanoparticles (HMSN@ASX) can improve the solubility of ASX and target delivery to the liver, significantly improving the therapeutic effects.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Nanoscience & Nanotechnology
Yue Qiao, Yangyang Li, Yang Ye, Yinhui Yu, Wei Wang, Ke Yao, Min Zhou
Summary: A multifunctional non antibiotic nanoplatform, Ga-mSiO2-BFN, has been reported for simultaneous eradication of multidrug-resistant Pseudomonas aeruginosa (MDR-PA) infection and alleviation of postcataract endophthalmitis (PCE) inflammation. The nanoplatform exhibits good cellular biocompatibility and demonstrates effective antibacterial mechanisms in in vivo and in vitro models.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Multidisciplinary
Dongxiao Zhang, Jian He, Jiarong Cui, Ruoxi Wang, Zhe Tang, Hongyu Yu, Min Zhou
Summary: This study constructs an orally used microalgae-nano integrated system (SP@ASXnano) by combining natural microalgae Spirulina platensis (SP) with astaxanthin nanoparticles (ASXnano), which effectively prevents radiation-induced intestinal and whole-body injury. The system shows enhanced drug delivery, anti-inflammatory effects, microbiota protection, and up-regulation of fecal short-chain fatty acids. It also ensures long-term biosafety.
Article
Nanoscience & Nanotechnology
Yuchen Qi, Yangyang Li, Kun Li, Tingting Xie, Shiyuan Hua, Qunfeng Guo, Yichun Zheng, Min Zhou
Summary: Researchers have developed gallium-based nanodots (GaNDs) that effectively treat multidrug-resistant Gram-negative bacterial infections by disrupting bacterial iron ability. These GaNDs significantly suppress the proliferation of two typical MDR bacteria strains and can also disrupt their biofilms. In animal models, GaNDs inhibit bacterial growth, reduce inflammation, and improve survival, demonstrating excellent biocompatibility and biosafety characteristics.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Chaojie Ren, Danni Zhong, Yuchen Qi, Chaoyi Liu, Xingyu Liu, Shihong Chen, Sheng Yan, Min Zhou
Summary: A microalgae-based oral insulin delivery strategy (CV@INS@ALG) was developed using Chlorella vulgaris (CV)-based insulin delivery system cross-linking with sodium alginate (ALG). This strategy could overcome the gastrointestinal barrier, protect insulin from harsh gastric conditions, and achieve a pH-responsive drug release in the intestine.
Article
Chemistry, Multidisciplinary
Chaoyi Liu, Qizhen Ye, Shiyuan Hua, Hui Huang, Danni Zhong, Feng Liang, Min Zhou
Summary: The research team has developed a multifunctional oral hydrogel system that can adsorb and remove lead from the body, alleviating lead poisoning-related diseases. This system can also repair the intestinal barrier, maintain intestinal homeostasis, and has advantages such as biodegradability, low cost, and high biosafety.
Article
Chemistry, Multidisciplinary
Jian He, Yang Ye, Dongxiao Zhang, Xiajing Tang, Chenqi Luo, Xiangjun Chen, Ke Yao, Min Zhou
Summary: This study demonstrates the successful recognition and eradication of MRSA using 4-mercaptophenylboronic acid-modified silver nanoparticles. The nanostructures effectively bind with MRSA cells and utilize released silver ions, while minimizing toxicity to mammalian cells. The antibacterial mechanism involves photocatalytic behavior and interference with bacterial genes. The nanostructures also allow for real-time monitoring of residual bacteria. This work holds significant potential for targeted MRSA treatment with clinical applications.
Review
Nanoscience & Nanotechnology
Danni Zhong, Zhen Du, Min Zhou
Summary: Microalgae, as a natural resource, have great potential in biomedical applications. Their unique morphological characteristics and easily functionalized surfaces make them ideal candidates for constructing novel biochemical probes, drug carriers, or biomedical scaffolds. Algae, with rich autofluorescent pigments, can be used for in situ oxygen production and biomedical imaging, offering promising applications in the diagnosis and therapies of hypoxia-associated diseases.
Article
Nanoscience & Nanotechnology
Wanlin Li, Yanling Wang, Yuchen Qi, Danni Zhong, Tingting Xie, Ke Yao, Shikuan Yang, Min Zhou
Summary: The study demonstrated a facile electrodeposition method to prepare silver peroxysulfite (AOHS) superpyramids with extraordinary antibacterial performance, showing highly efficient MDR bacteria-killing effect and long-term antibacterial activity. Moreover, evaporating a layer of copper onto the AOHS pyraminds can promote wound tissue angiogenesis, inhibit bacterial infection, and accelerate the healing process in MDR bacteria-infected wound.
ACS APPLIED BIO MATERIALS
(2021)
Article
Pharmacology & Pharmacy
Wanlin Li, Shoujie Wang, Danni Zhong, Zhen Du, Min Zhou
Summary: A bioactive living hydrogel based on photosynthetic microorganisms is designed for enhanced wound healing by production and local delivery of oxygen to alleviate acute and chronic tissue hypoxia. It is cost-effective, easy to obtain, and convenient for usage, showing promising potential for clinical application.
ADVANCED THERAPEUTICS
(2021)
