期刊
JOURNAL OF EXPERIMENTAL BOTANY
卷 67, 期 14, 页码 4105-4115出版社
OXFORD UNIV PRESS
DOI: 10.1093/jxb/erw189
关键词
CitAP2; ERF; Citrus sinensis; citrus volatile; ethylene; sweet orange; terpene synthase; TPS; transcriptional regulation; valencene
资金
- Program of International Science and Technology Cooperation [2011DFB31580]
- National Basic Research Program of China [2011CB100602]
- National Natural Science Foundation of China [31372010]
CitAP2.10 is a novel AP2/ERF transcription factor associated with (+)-valencene content in sweet orange fruit and operates by modulating CsTPS1 transcript abundance.Aroma is a vital characteristic that determines the quality and commercial value of citrus fruits, and characteristic volatiles have been analyzed in different citrus species. In sweet orange, Citrus sinensis, the sesquiterpene (+)-valencene is a key volatile compound in the fruit peel. Valencene synthesis is catalyzed by the terpene synthase CsTPS1, but the transcriptional mechanisms controlling its gene expression are unknown. Here, the AP2/ERF (APETALA2/ethylene response factor) transcription factor, CitAP2.10, is characterized as a regulator of (+)-valencene synthesis. The expression pattern of CitAP2.10 was positively correlated with (+)-valencene content and CsTPS1 expression. Dual-luciferase assays indicated that CitAP2.10 could trans-activate the CsTPS1 promoter. Ethylene enhanced expression of CitAP2.10 and this effect was abolished by the ethylene antagonist 1-methylcyclopropene. The role and function of CitAP2.10 in (+)-valencene biosynthesis were confirmed using the Arabidopsis homolog (AtWRI1), which also transiently activated the CsTPS1 promoter. Furthermore, transient over-expression of CitAP2.10 triggered (+)-valencene biosynthesis in sweet orange fruit. These results indicate that CitAP2.10 regulates (+)-valencene synthesis via induction of CsTPS1 mRNA accumulation.
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