Article
Engineering, Manufacturing
Mahdiyar Shahbazi, Henry Jaeger, Rammile Ettelaie
Summary: This study investigates the effects of printer temperature on the molecular behavior of starch during 3D printing. The extrusion-based printer degrades the starch chains, while the laser powder bed fusion printer promotes crosslinking/branching. The former reduces the structural strength of starch networks but increases digestibility rate, while the latter increases molecular size and decreases digestibility rate.
ADDITIVE MANUFACTURING
(2022)
Article
Construction & Building Technology
Manal Bouasria, Yassine El Mendili, Mohammed-Hichem Benzaama, Valerie Pralong, Jean-Francois Bardeau, Franck Hennequart
Summary: The incorporation of seaweed in building materials improves heat storage capacity and thermal insulation without compromising compressive strength. The most effective seaweed content for enhancing thermal insulation is 20%, which can significantly reduce thermal conductivity.
CONSTRUCTION AND BUILDING MATERIALS
(2021)
Article
Polymer Science
Pieter-Jan Voorter, Manfred Wagner, Christine Rosenauer, Jinhuo Dai, Priya Subramanian, Alasdair Mckay, Neil R. Cameron, Jasper J. Michels, Tanja Junkers
Summary: Diffusion-ordered NMR spectroscopy (DOSY) is a solvent-independent method for molecular weight calibration with low lab variability. It has been used for data calibration in different solvents and confirmed the inter-laboratory and inter-polymer consistency. It can also determine the molecular weight of polymers with added complexity.
Article
Chemistry, Analytical
Markus Matz, Carlo Botha, Timo Beskers, Manfred Wilhelm
Summary: Methods to increase sensitivity are important in research. In this study, a continuous injection method with a sinusoidal analyte concentration profile was developed to enhance sensitivity in HPLC. The proposed method was established and evaluated for SEC, and the phase angle shift was utilized for molecular weight determination.
Article
Food Science & Technology
Diane Purcell, Michael A. Packer, Maria Hayes
Summary: Seaweeds have been traditionally used as food and medicine in Asian cultures. This study focuses on the potential of extracting bioactive peptides from brown seaweed Laminaria digitata to inhibit the ACE-1 enzyme, which causes hypertension. Proteins were extracted from L. digitata using enzymatic hydrolysis, resulting in hydrolysates and permeates that showed significant ACE-1 inhibitory effects.
Article
Agricultural Engineering
Frederik J. Wolfaardt, Thierry Ghislain, Maxime Lessard, Johann F. Gorgens, Jean -Michel Lavoie
Summary: The two-step concentrated acid hydrolysis process is highly efficient in producing fermentable sugars from lignocellulosic biomass. Acid recovery is crucial for the economic viability of the process. This study used size-exclusion chromatography to analyze the molecular size distribution of water-soluble sugar molecules generated during the acid swelling of lignocellulosic biomass. Changes in the distribution indicators could be linked to operating conditions and downstream hydrolysis performance, providing valuable information for optimizing the hydrolysis process and acid recovery.
BIOMASS & BIOENERGY
(2023)
Article
Chemistry, Analytical
Naga Arjun Sakthivel, Vijay Reddy Jupally, Senthil Kumar Eswaramoorthy, Kalpani Hirunika Wijesinghe, Praneeth Reddy Nimmala, Chanaka Kumara, Milan Rambukwella, Tanya Jones, Amala Dass
Summary: The study demonstrated the versatility and effectiveness of using size exclusion chromatography (SEC) for separating gold nanomolecules, especially in cases where sizes are similar. This method offers easier separation, wider size range separation, and can be improved by increasing column length.
ANALYTICAL CHEMISTRY
(2021)
Review
Polymer Science
Kai Philipps, Tanja Junkers, Jasper J. Michels
Summary: Size exclusion chromatography (SEC) based on direct homopolymer calibration is commonly used for molecular weight determination in macromolecular synthesis, but can lead to misinterpretation in block copolymer (BCP) characterization. A critical evaluation of SEC is important to avoid false conclusions in experiments, and researchers may benefit from using alternative detection and characterization methods for accurate BCP characterization.
Article
Chemistry, Analytical
Iro Konstantina Ventouri, Wayne Chang, Florian Meier, Roland Drexel, Govert W. Somsen, Peter J. Schoenmakers, Bart de Spiegeleer, Rob Haselberg, Alina Astefanei
Summary: We developed an online analytical platform combining asymmetrical flow field-flow fractionation (AF4) with native mass spectrometry (nMS) for studying labile higher-order structures (HOS) of protein biotherapeutics. The platform also utilizes UV absorbance, multi-angle light scattering (MALS), and differential refractive-index (UV-MALS-dRI) detectors. We studied the stability and dissociation pathways of the tetrameric biotherapeutic enzyme L-asparaginase (ASNase) and found the presence of intact octamers and degradation products using AF4-MALS/nMS. Exposure of ASNase to high pH disrupted the equilibrium between non-covalent species and led to HOS dissociation. The developed platform provides valuable insights for aggregation and stability studies of protein biopharmaceuticals.
ANALYTICAL CHEMISTRY
(2023)
Article
Microbiology
Stephen A. Jackson, Maohang Duan, Pengyan Zhang, Maureen W. Ihua, Dagmar B. Stengel, Delin Duan, Alan D. W. Dobson
Summary: Epiphytic bacteria on macroalgae play important symbiotic roles in host morphogenesis, nutrient exchange, and protection. They possess valuable carbohydrates and enzymes that can be used in industrial bioprocessing.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemical Research Methods
Wouter C. Knol, Till Gruendling, Peter J. Schoenmakers, Bob W. J. Pirok, Ron A. H. Peters
Summary: The chain sequence of copolymers plays a crucial role in determining their physical properties. However, there are limited analytical methods available for monitoring the sequence of copolymers. In this study, the authors investigated the use of pyrolysis - gas chromatography (Py-GC) as an alternative method and successfully combined it with nuclear-magnetic-resonance spectroscopy (NMR) to obtain quantitative information on the chain sequence and composition of random and block copolymers.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Review
Virology
Hussein Kaddour, Malik Tranquille, Chioma M. Okeoma
Summary: Extracellular vesicles (EVs) are cell-derived membranous particles that carry and transport bioactive cargoes to recipient cells, playing important roles in physiological and pathophysiological processes. Efficient protocols for EV separation are currently lacking, but SEC-based methods show promise and future development of PPLC system for improved separation is suggested.
Article
Multidisciplinary Sciences
Monica Costa, Luis Pio, Pedro Bule, Vania Cardoso, Cristina M. Alfaia, Diogo Coelho, Joana Bras, Carlos M. G. A. Fontes, Jose A. M. Prates
Summary: This study demonstrated the effectiveness of an alginate lyase from the PL7 family in partially degrading the L. digitata cell wall, releasing beneficial nutritional compounds and improving bioavailability for monogastric animal feed.
SCIENTIFIC REPORTS
(2021)
Article
Polymer Science
Hamza Mahmoud Aboelanin, Jan-Hendrik Arndt, Tibor Macko, Stepan Podzimek, Robert Bruell
Summary: Characterization of the molar mass distribution of poly(ether ether ketone) (PEEK) is difficult due to its insolubility in common solvents. Sulfonated PEEK (SPEEK) was prepared by sulfonation of PEEK with concentrated H2SO4 and its presence of sulfonic acid group in PEEK matrix was confirmed. The molar mass and distribution of SPEEK were determined in different solvents using calibration with PMMA standards.
JOURNAL OF APPLIED POLYMER SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Claire Gazaille, Marion Sicot, Marthe Akiki, Nolwenn Lautram, Aurelien Dupont, Patrick Saulnier, Joel Eyer, Guillaume Bastiat
Summary: Characterizing the complex nanoassemblies is crucial for the translational approaches of nanomedicines, as demonstrated in a case study of NFL peptide adsorption. Using a chromatographic apparatus for characterization improved accuracy and reproducibility, showing potential for interactions between nanosystems and biological agents.
PHARMACEUTICAL RESEARCH
(2021)