Leptin receptor signaling sustains metabolic fitness of alveolar macrophages to attenuate pulmonary inflammation

Alveolar macrophages (AMs) are critical mediators of pulmonary inflammation. Given the unique lung tissue environment, whether there exist AM-specific mechanisms that control inflammation is not known. Here, we found that among various tissue-resident macrophage populations, AMs specifically expressed Lepr, encoding receptor for a key metabolic hormone leptin. AM-intrinsic Lepr signaling attenuated pulmonary inflammation in vivo, manifested as subdued acute lung injury yet compromised host defense against Streptococcus pneumoniae infection. Lepr signaling protected AMs from necroptosis and thus constrained neutrophil recruitment and tissue damage secondary to release of proinflammatory cytokine interleukin-1 alpha. Mechanistically, Lepr signaling sustained activation of adenosine monophosphate-activated protein kinase in a Ca2+ influx-dependent manner and rewired cellular metabolism, thus preventing excessive lipid droplet formation and overloaded metabolic stress in a lipid-rich alveolar microenvironment. In conclusion, our results defined AM-expressed Lepr as a metabolic checkpoint of pulmonary inflammation and exemplified a macrophage tissue adaptation strategy for maintenance of immune homeostasis.

Automated summary

Alveolar macrophages (AMs) play a crucial role in controlling pulmonary inflammation. They express Lepr, a receptor for the metabolic hormone leptin, which helps attenuate inflammation and protect against lung injury. However, this signaling also compromises the host defense against Streptococcus pneumoniae infection. Lepr signaling in AMs protects against necroptosis and limits excessive inflammation by regulating the release of proinflammatory cytokines.