期刊
APPLIED SCIENCES-BASEL
卷 12, 期 14, 页码 -出版社
MDPI
DOI: 10.3390/app12146889
关键词
cowpea; fusarium wilt; GWAS; candidate genes
类别
资金
- Key R&D Program of Guangdong Province [2020B020220002]
- National Key R&D Program of China [2017YFE0114500]
- Major Science and Technology Project of Plant Breeding in Zhejiang Province [2021C02065-6-3]
- Lingyan Project of Science and Technology Department of Zhejiang Province [2022C02051]
- Opening Project Fund of Key Laboratory of Biology and Genetic Resources of Rubber Tree
- Ministry of Agriculture and Rural Affairs
- P. R. China/State Key Laboratory Breeding Base of Cultivation and Physiology for Tropical Crops/Danzhou Investigation and Experiment Station of Tropical Crops, Ministry of Agriculture and Rural Affairs, P. R. China [RRI-KLOF202102]
- Natural Science Foundation of Zhejiang province [LY21C150002, LQY19C150001]
- National Natural Science Foundation of China [31872105, 31972221, 32002048, 31801862, 32172595]
- National Key Research and Development Program of China [2018YFD1000800, 2019YFD1000300]
A study on genetic diversity of 99 cowpea accessions infected by Fusarium wilt revealed 7 resistant phenotypes and identified 3 SNPs associated with leaf damage and 7 SNPs associated with vascular discoloration. The results provide insights for molecular breeding of Fusarium wilt resistant cowpea cultivars.
Fusarium wilt (FW), caused by the soil-borne fungal pathogen Fusarium oxysporum f. sp. Tracheiphilum, is a serious threat to cowpea production worldwide. Understanding the genetic architecture of FW resistance is a prerequisite to combatting this disease and developing FW resistance varieties. In the current study, a genetic diversity panel of 99 cowpea accessions was collected, and they were infected by a single strain, FW-HZ. The disease index (DI) based on the two indicators of leaf damage (LFD) and vascular discoloration (VD) varied highly across the population: most accessions were susceptible, and only seven accessions showed resistant phenotypes by both indicators. Through a genome-wide association study (GWAS), 3 and 7 single nucleotide polymorphisms (SNPs) significantly associated with LFD and VD were detected, respectively, which were distributed on chromosomes 3, 4, 5, 6 and 9, accounting for 0.68-13.92% of phenotypic variation. Based on the cowpea reference genome, 30 putative genes were identified and proposed as the likely candidates, including leucine-rich repeat protein kinase family protein, protein kinase superfamily protein and zinc finger family protein. These results provide novel insights into the genetic architecture of FW resistance and a basis for molecular breeding of FW resistant cultivars in cowpea.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据