期刊
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
卷 781, 期 -, 页码 351-355出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jelechem.2016.10.031
关键词
Electrochemiluminescent biosensor; Hydrazide modified graphene quantum dots; Hemin/G-quadruplex DNAzyme; p53 gene
资金
- National Natural Science Foundation of China [21475062, 21505077]
Graphene quantum dots (GQDs), an alternative of conventional luminescent reagents, has been widely used in electrochemiluminescent (ECL) analysis because of its low cost, non-toxicity and ease of preparation. To further improve the ECL signal of GQDs, we herein succeeded in synthesizing hydrazide modified graphene quantum dots (HM-SGQDs). In comparison with GQDs, HM-SGQDs possessed abundant luminol like units, thus greatly enhancing the ECL intensity. On the other hand, luminol involved ECL system typically needed the participation of H2O2 as a coreactant, while hemin/G-quadruplex DNAzyme (hGQ) DNAzyme could efficiently catalyze the decomposition of H2O2. Accordingly, taking p53 gene as a model, a novel ECL DNA biosensor was developed based on HM-SGQDs and hGQ DNAzyme. The experimental results indicated target DNA can be quantified in a linear range from 100 fM to 100 nM with a detection limit of 66 fM (S/N = 3). Meanwhile, the discrimination of single-base mismatch was also achieved with the proposed analytical approach, suggesting broad potential applications of nucleic acid-related clinical diagnosis. (C) 2016 Elsevier B.V. All rights reserved.
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