Multiple Substrate Binding Mode-Guided Engineering of a Thermophilic PET Hydrolase

Substrate-Binding Mode of a Thermophilic PET Hydrolase and Engineering the Enzyme to Enhance the Hydrolytic Efficacy

(2022) Wei Zeng et al.   ACS Catalysis

Machine learning-aided engineering of hydrolases for PET depolymerization

(2022) Hongyuan Lu et al.   NATURE

Mechanism-Based Design of Efficient PET Hydrolases

(2022) Ren Wei et al.   ACS Catalysis

Influence of substrate crystallinity and glass transition temperature on enzymatic degradation of polyethylene terephthalate (PET)

(2022) Thore Bach Thomsen et al.   New Biotechnology

Biocatalysis in the recycling landscape for synthetic polymers and plastics towards circular textiles

(2021) Christina Jönsson et al.   ChemSusChem

Computational Redesign of a PETase for Plastic Biodegradation under Ambient Condition by the GRAPE Strategy

(2021) Yinglu Cui et al.   ACS Catalysis

An absorbance method for analysis of enzymatic degradation kinetics of poly(ethylene terephthalate) films

(2021) En Ze Linda Zhong-Johnson et al.   Scientific Reports

The Current State of Research on PET Hydrolyzing Enzymes Available for Biorecycling

(2021) Fusako Kawai   Catalysts

Low Carbon Footprint Recycling of Post‐Consumer PET Plastic with a Metagenomic Polyester Hydrolase

(2021) Christian Sonnendecker et al.   ChemSusChem

QM/MM Study of the Enzymatic Biodegradation Mechanism of Polyethylene Terephthalate

(2021) Sergio Boneta et al.   Journal of Chemical Information and Modeling

Enzymatic degradation of polyethylene terephthalate nanoplastics analyzed in real time by isothermal titration calorimetry

(2021) Kristina Vogel et al.   SCIENCE OF THE TOTAL ENVIRONMENT

Reaction Mechanism of the PET Degrading Enzyme PETase Studied with DFT/MM Molecular Dynamics Simulations

(2021) Carola Jerves et al.   ACS Catalysis

Positive Charge Introduction on the Surface of Thermostabilized PET Hydrolase Facilitates PET Binding and Degradation

(2021) Akihiko Nakamura et al.   ACS Catalysis

IsPETase- and IsMHETase-Catalyzed Cascade Degradation Mechanism toward Polyethylene Terephthalate

(2021) Shanshan Feng et al.   ACS Sustainable Chemistry & Engineering

Techno-economic, life-cycle, and socioeconomic impact analysis of enzymatic recycling of poly(ethylene terephthalate)

(2021) Avantika Singh et al.   Joule

Comparative Performance of PETase as a Function of Reaction Conditions, Substrate Properties, and Product Accumulation

(2021) Erika Erickson et al.   ChemSusChem

Engineering and evaluation of thermostable Is PETase variants for PET degradation

(2021) Stefan Brott et al.   ENGINEERING IN LIFE SCIENCES

Computational biotransformation of polyethylene terephthalate by depolymerase: A QM/MM approach

(2021) Mingna Zheng et al.   JOURNAL OF HAZARDOUS MATERIALS

Kinetic Modeling of the Post-consumer Poly(Ethylene Terephthalate) Hydrolysis Catalyzed by Cutinase from Humicola insolens

(2021) Erika de Queiros Eugenio et al.   JOURNAL OF POLYMERS AND THE ENVIRONMENT

The metabolic potential of plastics as biotechnological carbon sources – Review and targets for the future

(2021) Till Tiso et al.   METABOLIC ENGINEERING

An engineered PET depolymerase to break down and recycle plastic bottles

(2020) V. Tournier et al.   NATURE

UV Pretreatment Impairs the Enzymatic Degradation of Polyethylene Terephthalate

(2020) Patricia Falkenstein et al.   Frontiers in Microbiology

Possibilities and limitations of biotechnological plastic degradation and recycling

(2020) Ren Wei et al.   Nature Catalysis

Structural basis of mutants of PET ‐degrading enzyme from Saccharomonospora viridis AHK190 with high activity and thermal stability

(2020) Miho Emori et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Biocatalytic Degradation Efficiency of Postconsumer Polyethylene Terephthalate Packaging Determined by Their Polymer Microstructures

(2019) Ren Wei et al.   Advanced Science

Efficient Degradation of Poly(ethylene terephthalate) with Thermobifida fusca Cutinase Exhibiting Improved Catalytic Activity Generated using Mutagenesis and Additive-based Approaches

(2019) Makoto Furukawa et al.   Scientific Reports

Conformational fitting of a flexible oligomeric substrate does not explain the enzymatic PET degradation

(2019) Ren Wei et al.   Nature Communications

Characterization and engineering of a plastic-degrading aromatic polyesterase

(2018) Harry P. Austin et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Enzymatic hydrolysis of PET: functional roles of three Ca2+ ions bound to a cutinase-like enzyme, Cut190*, and its engineering for improved activity

(2018) Masayuki Oda et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Structural Dynamics of the PET-Degrading Cutinase-like Enzyme from Saccharomonospora viridis AHK190 in Substrate-Bound States Elucidates the Ca2+-Driven Catalytic Cycle

(2018) Nobutaka Numoto et al.   BIOCHEMISTRY

Structural studies reveal the molecular mechanism of PETase

(2018) Chun-Chi Chen et al.   FEBS Journal

Enzymatic recovery of polyester building blocks from polymer blends

(2017) Caroline Gamerith et al.   PROCESS BIOCHEMISTRY

Structural insight into catalytic mechanism of PET hydrolase

(2017) Xu Han et al.   Nature Communications

Engineered bacterial polyester hydrolases efficiently degrade polyethylene terephthalate due to relieved product inhibition

(2016) Ren Wei et al.   BIOTECHNOLOGY AND BIOENGINEERING

A dual enzyme system composed of a polyester hydrolase and a carboxylesterase enhances the biocatalytic degradation of polyethylene terephthalate films

(2016) Markus Barth et al.   Biotechnology Journal

A bacterium that degrades and assimilates poly(ethylene terephthalate)

(2016) Shosuke Yoshida et al.   SCIENCE

A dual enzyme system composed of a polyester hydrolase and a carboxylesterase enhances the biocatalytic degradation of polyethylene terephthalate films

(2016) Markus Barth et al.   Biotechnology Journal

Effect of Tris, MOPS, and phosphate buffers on the hydrolysis of polyethylene terephthalate films by polyester hydrolases

(2016) Juliane Schmidt et al.   FEBS Open Bio

A disulfide bridge in the calcium binding site of a polyester hydrolase increases its thermal stability and activity against polyethylene terephthalate

(2016) Johannes Then et al.   FEBS Open Bio

Ca2+and Mg2+binding site engineering increases the degradation of polyethylene terephthalate films by polyester hydrolases fromThermobifida fusca

(2015) Johannes Then et al.   Biotechnology Journal

Ca2+and Mg2+binding site engineering increases the degradation of polyethylene terephthalate films by polyester hydrolases fromThermobifida fusca

(2015) Johannes Then et al.   Biotechnology Journal

Structural basis for the Ca2+-enhanced thermostability and activity of PET-degrading cutinase-like enzyme from Saccharomonospora viridis AHK190

(2014) Takuya Miyakawa et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Crystal Structure and Thermodynamic and Kinetic Stability of Metagenome-Derived LC-Cutinase

(2014) Sintawee Sulaiman et al.   BIOCHEMISTRY

Functional characterization and structural modeling of synthetic polyester-degrading hydrolases from Thermomonospora curvata

(2014) Ren Wei et al.   AMB Express

Turbidimetric analysis of the enzymatic hydrolysis of polyethylene terephthalate nanoparticles

(2013) Ren Wei et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Isolation of a Novel Cutinase Homolog with Polyethylene Terephthalate-Degrading Activity from Leaf-Branch Compost by Using a Metagenomic Approach

(2011) Sintawee Sulaiman et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Identification and comparison of cutinases for synthetic polyester degradation

(2011) Peter James Baker et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Engineered Thermobifida fusca cutinase with increased activity on polyester substrates

(2011) Carla Silva et al.   Biotechnology Journal

Cutinase-Catalyzed Hydrolysis of Poly(ethylene terephthalate)

(2009) Åsa M. Ronkvist et al.   MACROMOLECULES