Article
Multidisciplinary Sciences
Hanh M. Truong, Kevin O. Cruz-Colon, Jorge Y. Martinez-Marquez, Jason R. Willer, Amanda M. Travis, Sondip K. Biswas, Woo-Kuen Lo, Hanno J. Bolz, Jillian N. Pearring
Summary: The tectonic complex acts as a barrier in the photoreceptor cilium to prevent unsolicited protein entry, ensuring proper functioning of the photoreceptor.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Kwangjin Park, Chunmei Li, Sofia Tsiropoulou, Joao Goncalves, Christine Kondratev, Laurence Pelletier, Oliver E. Blacque, Michel R. Leroux
Summary: Cilia play essential roles in cell motility, fluid flow, and signaling, and are divided into subcompartments with variable structures and functions. CDKL-1, a kinase related to the human CDKL family, controls the length of a specific ciliary subdomain and influences cilium-dependent processes. Overall, cilium length is regulated by various pathways and CDKL-1 is crucial for the construction of a specific ciliary compartment and contributes to development and sensory physiology.
Article
Medicine, Research & Experimental
Chao Xie, Julien C. Habif, Kirill Ukhanov, Cedric R. Uytingco, Lian Zhang, Robert J. Campbell, Jeffrey R. Martens
Summary: Ciliopathies are a class of genetic diseases that cause dysfunction in cilia in multiple organ systems, including the olfactory system. This study investigates the mechanism behind the reduction of olfactory cilia in ciliopathy Bardet-Biedl syndrome (BBS) and finds that phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) and F-actin play important roles in cilia shortening. The researchers successfully restored cilia length and rescued odor detection and perception in BBS using a gene therapeutic approach. These findings provide valuable insights for the treatment of ciliopathies.
Article
Cell Biology
Andrew Ruba, Mark Tingey, Wangxi Luo, Jingjie Yu, Athanasios Evangelou, Rachel Higgins, Saovleak Khim, Weidong Yang
Summary: Transport of membrane and cytosolic proteins into the primary cilium is essential for its role in cellular signaling. Using virtual three-dimensional superresolution light microscopy, researchers have discovered new pathways, passive diffusion and vesicle-assisted transport, for the transportation of proteins from the cytoplasm to the primary cilium.
MOLECULAR BIOLOGY OF THE CELL
(2023)
Review
Urology & Nephrology
Ailis L. Moran, Laura Louzao-Martinez, Dominic P. Norris, Dorien J. M. Peters, Oliver E. Blacque
Summary: Primary cilia serve as cell surface antennae, coordinating cellular responses to sensory inputs and signaling molecules that regulate development and homeostasis. The compartmentalized structure of cilia, along with active transport pathways and gating mechanisms, plays a crucial role in maintaining its unique molecular composition and signaling environment. Understanding the major compartmentalizing pathways at the cilium has provided insights into ciliary disease and associated renal disorders.
NATURE REVIEWS NEPHROLOGY
(2023)
Article
Multidisciplinary Sciences
Ran He, Guanhao Chen, Zhiwei Li, Jianchao Li
Summary: In this study, it was found that the N-terminal coil structure of Rpgrip1l is made up of two parallel dimers. Overexpression of these dimers significantly shortened the length of primary cilia, and this effect was dependent on the formation of dimers. It was also observed that the N-terminal structure of Rpgrip1 in mouse and human differed significantly from Rpgrip1l. Furthermore, disease-related mutations were found to alter the dimeric states of Rpgrip1l or Rpgrip1, possibly explaining the mechanisms behind the associated pathologies.
Article
Biochemistry & Molecular Biology
Shichao Duan, Hao Li, Yirong Zhang, Suming Yang, Yawen Chen, Benhua Qiu, Cheng Huang, Juan Wang, Jinsong Li, Xueliang Zhu, Xiumin Yan
Summary: This study reveals the molecular switch function of small GTPase Rabl2 in the passage through the ciliary barrier transition zone, where GTP hydrolysis regulates the outward passage of BBSome and its cargos with retrograde IFT machinery crucial for proper ciliary signaling.
Article
Cell Biology
Leah Schembs, Ariane Willems, Kerstin Hasenpusch-Theil, James D. Cooper, Katie Whiting, Karen Burr, Sunniva M. K. Bostrand, Bhuvaneish T. Selvaraj, Siddharthan Chandran, Thomas Theil
Summary: This study reveals the importance of primary cilia in dorsal and ventral patterning in human corticogenesis, identifies the tissue-specific role of the INPP5E gene as a negative regulator of SHH signaling, and suggests implications for the pathogenesis of neurodevelopmental disorders.
Article
Genetics & Heredity
Swadhin Chandra Jana, Priya Dutta, Akanksha Jain, Anjusha Singh, Lavanya Adusumilli, Mukul Girotra, Diksha Kumari, Seema Shirolikar, Krishanu Ray
Summary: Cilia, antenna-like organelles of eukaryotic cells, play important roles in cellular signaling. Research shows that the entry of Orco into the cilia on olfactory sensory neurons in fruit flies is restricted to a narrow developmental window. Heterotrimeric kinesin-2 is critical for functionalizing olfactory cilia and interacts with Orco to transport it into the cilium.
Review
Cell Biology
K. Bouhouche, M. S. Valentine, P. Le Borgne, M. Lemullois, J. Yano, S. Lodh, A. Nabi, A. M. Tassin, J. L. Van Houten
Summary: Cilia are ubiquitous and highly conserved extensions that endow the cell with motility and sensory functions. Paramecium, a ciliated organism, is a suitable model for studying cilia assembly and function, and can be used to validate candidate genes for ciliopathies.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Kwangjin Park, Michel R. Leroux
Summary: The transition zone (TZ) of the cilium acts as a diffusion barrier, ensuring ciliary autonomy and homeostasis by its unique complement and spatial organization of proteins, a protein picket fence, a specialized lipid microdomain, differential membrane curvature and thickness, and a size-selective molecular sieve. Understanding the TZ is important not only for illuminating essential aspects of human cell signaling, physiology, and development, but also for unraveling how TZ dysfunction contributes to ciliopathies affecting multiple organ systems.
Article
Cell Biology
Maria Chatzifrangkeskou, Panayiotis Kouis, Paris A. Skourides
Summary: Chatzifrangkeskou et al. discovered that c-Jun N-terminal kinase (JNK) plays an important role in ciliogenesis and ciliary function by regulating the actin networks in multiciliated cells. JNK signaling is critical for maintaining the actin networks and ciliary function in mature multiciliated cells. Additionally, JNK is implicated in the development of diseases such as diabetes, neurodegeneration, and liver disease, which are associated with ciliary dysfunction.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Cell Biology
Yingyi Zhang, Jielu Hao, Mariana G. Tarrago, Gina M. Warner, Nino Giorgadze, Qing Wei, Yan Huang, Kai He, Chuan Chen, Thais R. Peclat, Thomas A. White, Kun Ling, Tamar Tchkonia, James L. Kirkland, Eduardo N. Chini, Jinghua Hu
Summary: Primary cilia play a central role in the determination of preadipocyte fate and the generation of metabolically healthy fat tissue by controlling transition fiber processes and signaling pathways, impacting adipocyte differentiation, obesity, and metabolic health.
Article
Ophthalmology
Mariia Dvoriashyna, Alexander J. E. Foss, Eamonn A. Gaffney, Rodolfo Repetto
Summary: This article presents a mathematical model for predicting the production rate and composition of aqueous humor (AH) in the eye. The model provides insights into the controlling parameters and potential manipulation strategies for regulating aqueous production. Key factors impacting aqueous production rate are identified, including cell membrane permeability, ion transporters, and carbonic anhydrase.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Review
Food Science & Technology
Bhanu Devnani, Galo Chuchuca Moran, Lutz Grossmann
Summary: In recent years, brewer's spent grain (BSG) has gained attention as a large quantity plant-based protein source from beer brewing by-products. The proteins in BSG, extraction techniques, and conditions have been studied to understand their composition and properties. BSG contains mainly water-insoluble hordeins and glutelins, with the protein isolates showing a broad molecular weight distribution. Chemical extraction methods offer potential for gelled food products, while physical and enzymatic modifications can produce soluble and functional protein isolates suitable for various applications, highlighting the value of BSG as a protein source.
Article
Biotechnology & Applied Microbiology
Weijie Liu, Chenchu Zhao, Jihong Jiang, Qian Lu, Yan Hao, Liang Wang, Cong Liu
BIOTECHNOLOGY FOR BIOFUELS
(2015)
Article
Biology
Muqing Cao, Jue Ning, Carmen I. Hernandez-Lara, Olivier Belzile, Qian Wang, Susan K. Dutcher, Yanjie Liu, William J. Snell
Article
Biochemical Research Methods
Liang Wang, Fengyuan Yang, Huiyi Chen, Zhiyue Fan, Yongkang Zhou, Jun Lu, Yuanlin Zheng
Article
Multidisciplinary Sciences
Lili Li, Guangmei Tian, Hai Peng, Dan Meng, Liang Wang, Xiao Hu, Cheng Tian, Miao He, Junfei Zhou, Lihong Chen, Cheng Fu, Weixiong Zhang, Zhangfeng Hu
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Biochemistry & Molecular Biology
Liang Wang, Lijing Yang, Xin Wen, Zhuoya Chen, Qiaoying Liang, Jialing Li, Wen Wang
BIOSCIENCE REPORTS
(2019)
Article
Genetics & Heredity
Minna Luo, Li Cao, Zongfu Cao, Siyu Ma, Yue Shen, Di Yang, Chao Lu, Zaisheng Lin, Zhimin Liu, Yufei Yu, Ruikun Cai, Cuixia Chen, Huafeng Gao, Xueyan Wang, Muqing Cao, Xu Ma
MOLECULAR GENETICS & GENOMIC MEDICINE
(2019)
Article
Genetics & Heredity
Minna Luo, Ruida He, Zaisheng Lin, Yue Shen, Guangyu Zhang, Zongfu Cao, Chao Lu, Dan Meng, Jing Zhang, Xu Ma, Muqing Cao
FRONTIERS IN GENETICS
(2020)
Article
Genetics & Heredity
Minna Luo, Zaisheng Lin, Tian Zhu, Minjun Jin, Dan Meng, Ruida He, Zongfu Cao, Yue Shen, Chao Lu, Ruikun Cai, Yong Zhao, Xueyan Wang, Hui Li, Shijing Wu, Xuan Zou, Guanjun Luo, Li Cao, Min Huang, Huike Jiao, Huafang Gao, Ruifang Sui, Chengtian Zhao, Xu Ma, Muqing Cao
Summary: The study identified IFT74 as a gene associated with JBTS, and revealed pathological effects including attenuated ciliogenesis, altered distribution of IFT proteins and ciliary membrane proteins, and disrupted hedgehog signaling in patients with specific variants.
GENETICS IN MEDICINE
(2021)
Article
Cell Biology
Min Huang, Xinlong Kong, Zaiming Tang, Zaisheng Lin, Ruida He, Muqing Cao, Xiujuan Zhang
Summary: The protein TCHP plays a crucial role in cell cycle progression by disassembling cilia, and its depletion leads to cell cycle arrest in G(0) phase, which cannot be reversed by blocking cilia assembly. Additionally, TCHP-induced cell cycle arrest is not mediated by centrosome surveillance mechanism, but requires inhibition of Rb or both Rb and p53 signaling pathways to reverse the phenotype.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Cell Biology
Yi-Ling Qiu, Li Wang, Min Huang, Min Lian, Fengbin Wang, Ying Gong, Xiong Ma, Chen-Zhi Hao, Jing Zhang, Zhong-Die Li, Qing-He Xing, Muqing Cao, Jian-She Wang
Summary: In this study, we identified TMEM67 gene variants associated with a mild phenotype of cholestasis in patients without typical symptoms of TMEM67 defects.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Panpan Zhu, Pengfei Zheng, Xinlong Kong, Shuo Wang, Muqing Cao, Chengtian Zhao
Summary: This study reveals the important role of Rassf7a in spinal cord regeneration and neural progenitor cell proliferation after spinal cord injury. Rassf7a modulates spindle orientation and cell cycle progression to affect neurogenesis.
Article
Biochemistry & Molecular Biology
Haibo Xie, Yunsi Kang, Junjun Liu, Min Huang, Zhicheng Dai, Jiale Shi, Shuo Wang, Lanqin Li, Yuan Li, Pengfei Zheng, Yi Sun, Qize C. Han, Jingjing Zhang, Zezhang Zhu, Leilei Xu, Pamela Yelick, Muqing Cao, Chengtian Zhao
Summary: Idiopathic scoliosis is a common spinal deformity in childhood, but its underlying pathogenesis is largely unknown. This study used zebrafish mutants to show that defects in ependymal cell polarity are one of the earliest signs of scoliosis, and further revealed the role of Urotensin signaling in scoliosis progression. These findings have implications for understanding the development of scoliosis in humans.
Article
Biochemistry & Molecular Biology
Daisuke Takao, Liang Wang, Allison Boss, Kristen J. Verhey