Article
Biochemistry & Molecular Biology
Eun Suh Kim, Youjin Baek, Hyun-Jae Yoo, Ji-Soo Lee, Hyeon Gyu Lee
Summary: This study demonstrated the enhanced stability, antioxidant activity, and bioavailability of astaxanthin through encapsulation within chitosan-tripolyphosphate nanoparticles. The nanoparticles prolonged the release of astaxanthin and improved its antioxidant properties. The results suggest the potential of chitosan-tripolyphosphate nanoparticles as a delivery system for astaxanthin.
Article
Pharmacology & Pharmacy
Li Shangyong, Zhang Hui, Chen Kaiwei, Jin Mengfei, Vu Son Hai, Samil Jung, He Ningning, Zheng Zhou, Myeong-Sok Lee
Summary: Oral drug delivery systems have advantages of simplicity and minimal side effects, making them suitable for colon-targeted therapy. Chitosan/alginate nanoparticles (CANPs) show potential in the treatment of intestinal diseases due to their strong electrostatic interaction and intestinal mucosal adhesion.
Article
Engineering, Biomedical
Weizhi Wang, Chenggong Yu, Fangfang Zhang, Yuxuan Li, Bo Zhang, Jie Huang, Zhijun Zhang, Liang Jin
Summary: A drug delivery system, GC-CA@PLGA NPs, has been developed to improve the oral administration of insulin with excellent stability, good biocompatibility, and significantly increased bioavailability for potential application in the treatment of diabetes.
BIOMEDICAL MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Jonathan Wang, Deborah Chin, Christopher Poon, Valeria Mancino, Jessica Pham, Hui Li, Pei-Yin Ho, Kenneth R. Hallows, Eun Ji Chung
Summary: Nanoparticles synthesized from chitosan showed promising mucoadhesive and permeation capabilities in vitro, with improved controlled release and serum delivery over 24 hours. Loading metformin into chitosan nanoparticles resulted in lower cyst burden in a PKD murine model, demonstrating good tolerance and kidney health. This study presents a novel, oral nanoformulation for chronic diseases like PKD, building upon previous chitosan-based drug delivery approaches.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Biochemistry & Molecular Biology
Mingze Xu, Jinpeng Huang, Siyuan Jiang, Jiecheng He, Zhen Wang, Han Qin, Yan-Qing Guan
Summary: Compared with injection, oral drug delivery is a preferred mode of administration due to its security, low pain, and simplicity. In this study, a targeted oral delivery system was developed using ConA-INS-KGM nanoparticles loaded with insulin, which demonstrated reversible glucose-responsive insulin release and high biocompatibility. These nanoparticles show significant potential for diabetes treatment and have various applications in biomedical fields.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Pharmacology & Pharmacy
Mona M. Elkhatib, Amir Ali, Ali S. Al-Badrawy
Summary: An advanced gut iontophoresis technology has been developed for delivering insulin nanoparticles across the gut wall, showing promising results in enhancing insulin transport and inducing intense hypoglycemic effects. Cell viability assays confirmed the non-toxic effects of nanoparticles on intestinal cells, suggesting that nanoparticle gut iontophoresis could be a potential non-invasive technique for oral insulin delivery.
BIOLOGICAL & PHARMACEUTICAL BULLETIN
(2021)
Article
Agriculture, Multidisciplinary
Niladri Sekhar Chatterjee, Pavan Kumar Dara, Sreerekha Perumcherry Raman, Divya K. Vijayan, Jayashree Sadasivam, Suseela Mathew, Chandragiri Nagarajarao Ravishankar, Rangasamy Anandan
Summary: This study developed and characterized black carrot anthocyanin-loaded chitosan nanoparticles (ACNPs) using the ionic gelation technique. The ACNPs showed improved in vivo antioxidant activity after nanoencapsulation, despite a slight reduction in in vitro antioxidant potential. The results suggest that ACNPs have potential as a nutraceutical supplement.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2021)
Article
Chemistry, Multidisciplinary
Melissa Kanzelberger Coolich, Olivia L. Lanier, Ethan Cisneros, Nicholas A. Peppas
Summary: This work demonstrates the great potential of H-bonded, pH-sensitive P(MAA-g-EG) gels as protein carriers. The use of poly(ethylene glycol) (PEG) chains promotes mucoadhesion in the small intestine, increasing the likelihood of drug release within the absorptive intestinal wall. PEGylation of insulin protects the drug from degradation and improves its stability. Cellular transport and changes in transepithelial resistance caused by PEGylated insulin (PI) were analyzed using a co-culture of Caco-2 and HT-29-MTX cells. The in vivo absorption of insulin in rats confirmed the biological activity of PEGylated insulin conjugates.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Nanoscience & Nanotechnology
Akbar Ali, Saroj Saroj, Sunita Saha, Sanjay Kumar Gupta, Tatini Rakshit, Suchetan Pal
Summary: In this study, chitosan nanoparticle/poly(vinyl alcohol) hybrid hydrogels (CPHGs) were developed for controlled insulin delivery for diabetes management. CPHGs exhibited reduced solid-like properties under low pH and high glucose environments. They also showed good self-healing and noncytotoxic properties.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Acoustics
Touseef Ahmed Wani, F. A. Masoodi, Rehana Akhter, Towseef Akram, Adil Gani, Nadeem Shabir
Summary: This research investigated the production of chitosan nanocapsules for encapsulating hydroxytyrosol. The resulting nanocapsules exhibited favorable packaging characteristics and encapsulation efficiency, and showed superior antioxidant and antiproliferative properties compared to free hydroxytyrosol.
ULTRASONICS SONOCHEMISTRY
(2022)
Article
Pharmacology & Pharmacy
Joanne Heade, Fiona McCartney, Miguel Chenlo, Olga Moreno Marro, Maja Severic, Robert Kent, Sinead B. Bleiel, Clara Alvarez, Brendan T. Griffin, David J. Brayden
Summary: The study aimed to develop a convenient oral delivery system for insulin and peptides, investigating the encapsulation and release properties of insulin entrapped in dWPI-based beads. Results showed promising potential for the dWPI-based beads as an oral peptide delivery system with high encapsulation efficiency and controlled release profile.
Article
Chemistry, Applied
Atefe Sadeghi Lari, Payam Zahedi, Hedayatollah Ghourchian, Alireza Khatibi
Summary: This study successfully synthesized novel crosslinked carboxymethyl chitosan nanoparticles using microfluidics and demonstrated their potential application in diabetes therapy. In the rat experiments, it was found that metformin hydrochloride loaded in these nanoparticles significantly increased body weight and reduced blood glucose levels, with effects on pancreatic islets and beta cells similar to healthy conditions.
CARBOHYDRATE POLYMERS
(2021)
Article
Biochemistry & Molecular Biology
Huda S. AlSalem, Nisreen M. Abdulsalam, Najla A. Khateeb, Mona S. Binkadem, Nahlah A. Alhadhrami, Abdalla M. Khedr, Rehab Abdelmonem, Kamel R. Shoueir, Eman Hassan Nadwa
Summary: Chitosan was modified and functionalized to improve oral insulin delivery. The Cs-DNS NPs had a particle size of 33.29 ± 5.08 nm and positive zeta potential. In vitro experiments showed high insulin entrapment efficiency and gradual release at different pH levels. In vivo experiments demonstrated that insulin-incorporated Cs-DNS NPs effectively lowered serum glucose levels, with a bioavailability of 17.5 ± 0.31% and pharmacological availability of 8.6 ± 0.8%.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Pharmacology & Pharmacy
Ayca Gungor Ak, Inci Turan, Hale Sayan Ozacmak, Aysegul Karatas
Summary: This study aimed to enhance the oral bioavailability and gastrointestinal stability of berberine chloride (BER) by preparing chitosan nanoparticles using an ionotropic gelation method. The BER-loaded chitosan nanoparticles showed an encapsulation efficiency of 31.5%, a zeta potential of 41.6 mV, a particle size of 406 nm, and a polydispersity index of 0.462. Stability studies demonstrated that the nanoparticles improved the stability of BER in simulated gastrointestinal fluids.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Article
Nanoscience & Nanotechnology
Xia Huang, Shangcong Han, Zuxian Chen, Lei Zhao, Changduo Wang, Qingyang Guo, Yanfeng Li, Yong Sun
Summary: LDH nanoparticles modified by DCA and HA improved the absorption efficiency of insulin by opening the TJs of cells and interacting with the cholic acid transporter receptor protein.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Article
Pharmacology & Pharmacy
Ezgi Oner, Mustafa Kotmakci, Ayse Gulten Kantarci
PHARMACEUTICAL DEVELOPMENT AND TECHNOLOGY
(2020)
Article
Chemistry, Medicinal
Huseyin Istanbullu, Gulsah Bayraktar, Hasan Akbaba, Ibrahim Cavus, Gunes Coban, Bilge Debelec Butuner, Ali Ahmet Kilimcioglu, Ahmet Ozbilgin, Vildan Alptuzun, Ercin Erciyas
ARCHIV DER PHARMAZIE
(2020)
Article
Chemistry, Multidisciplinary
Hasan Akbaba, Gulsah Erel-Akbaba, Mustafa Kotmakci, Yucel Baspinar
PHARMACEUTICAL RESEARCH
(2020)
Article
Cell Biology
Shannon K. Bromley, Hasan Akbaba, Vinidhra Mani, Rut Mora-Buch, Alexandra Y. Chasse, Andrea Sama, Andrew D. Luster
Article
Biotechnology & Applied Microbiology
Ezgi Oner, Mustafa Kotmakci, Anne-Marie Baird, Steven G. Gray, Bilge Debelec Butuner, Emir Bozkurt, Ayse Gulten Kantarci, Stephen P. Finn
Summary: Researchers successfully developed novel cationic solid lipid nanoparticles carrying siRNA targeting EphA2 receptor, which showed enhanced gene silencing when co-administered with a histone lysine demethylase inhibitor, JIB-04. The delivery system demonstrated high transfection efficiency and holds promise for targeting other genes and cancer types in further in vitro and in vivo studies.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Hasan Akbaba, Gulsah Erel-Akbaba, Serif Senturk
Summary: The study demonstrates the potential of solid lipid nanoparticles as a delivery system for the CRISPR/Cas9 gene-editing tool in anticancer therapies. Results show that these nanoparticles are nontoxic, stable, and have good transfection efficiency, making them a promising candidate for targeted anticancer properties.
Article
Pharmacology & Pharmacy
Gulsah Erel-Akbaba, Hasan Akbaba
Summary: The study successfully developed a lipoplex system for delivering FGF-2 encoding plasmid DNA, which demonstrated significant transfection and FGF-2 expression ability in vitro, promoting cell proliferation and migration.
DARU-JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Gulsah Erel-Akbaba, Selen Isar, Hasan Akbaba
Summary: Solid Witepsol nanoparticles prepared through microemulsion dilution technique are promising non-viral delivery systems for gene therapy, as they exhibit small particle sizes, high Zeta potential, and effective transfection ability. Successful delivery relies on careful selection of components and their amounts.
TURKISH JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Bilge Debelec-Butuner, Ezgi Oner, Mustafa Kotmakci, Ayse Gulten Kantarci
Summary: Inhibition of SIRT1 can enhance the activity of doxorubicin on prostate cancer cell lines by improving DNA damage recognition and promoting cancer cell death.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2021)
Article
Pharmacology & Pharmacy
Selen Isar, Hasan Akbaba, Yigit Sahin, Meric A. Altinoz, Ayse Nalbantsoy, Gulsah Erel-Akbaba, Yucel Baspinar
Summary: This study focused on the preparation and characterization of cationic nanoemulsions containing erucic acid (EA) and phytosphingosine (PS) for plasmid DNA (pDNA) delivery. It was found that NE 2 containing PS and EA is effective for delivering pDNA and has a synergistic effect on breast cancer cells.
PHARMACEUTICAL DEVELOPMENT AND TECHNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Gulsah Erel-Akbaba, Hasan Akbaba, Erdi Keselik, Selen Akyol Bahceci, Zeynep Senyigit, Tijen Kaya Temiz
Summary: An innovative protein delivery method was developed in this study, where basic fibroblast growth factor was nanoencapsulated into a gel system and combined with cell penetrating peptide octaarginine, effectively accelerating the healing of diabetic wounds.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
G. Kaftan, S. Sozer Karadagli, O. Sogut, G. Armagan
Article
Pharmacology & Pharmacy
Gulsah Erel-Akbaba, Hasan Akbaba
Summary: This study developed an optimal cationic liposome system with suitable physiochemical characteristics for gene delivery, showing effective DNA binding and protection against nucleases with low cytotoxicity. Furthermore, increasing the EQ mole ratio improved transfection ability while maintaining stability at low temperature, highlighting its potential as a nucleic acid delivery vehicle for gene therapy.
JOURNAL OF RESEARCH IN PHARMACY
(2021)
Article
Pharmacology & Pharmacy
Selen Isar, Hasan Akbaba, Gulsah Erel-Akbaba, Yucel Baspinar
JOURNAL OF RESEARCH IN PHARMACY
(2020)
Article
Pharmacology & Pharmacy
Xueliang Xiu, Shichao Ma, Zhiyong Li, Zhipeng Li, Xuemei Li, Yangtao Ruan, Dezhao Li, Jianwei Wang, Fengsen Ma
Summary: Liposomes are promising delivery vehicles for drugs and vaccines. The use of microfluidic chips for liposome preparation is an effective method. However, traditional liposome preparation technologies hinder their industrial production and clinical application. This study presents a novel three-dimensional integrated microfluidic device for continuous liposome preparation and purification, which shows potential for industrial production of nano-pharmaceutical applications.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2024)