期刊
TALANTA
卷 246, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.talanta.2022.123493
关键词
pH; Sensor; Alkaliphiles; Thermally activated delayed fluorescence; Time-resolved fluorescence imaging
资金
- National Natural Science Foundation of China (NSFC) [22122705, 22077139]
- Fundamental Research Funds
In this study, a thermally activated delayed fluorescent (TADF) pH probe, NI-D-OH, was synthesized for the first time. The probe demonstrates a good linear relationship between fluorescence intensity and pH in the neutral to alkaline range (pH 7.0-8.6), as well as long-lived TADF emission. It was further shown that NI-D-OH can be used to monitor intracellular pH in living organisms and evaluate the effect of Na+ on pH homeostasis.
pH homeostasis is essential for alkaliphiles, given their widespread use in biotechnological applications. However, quantitative monitoring of alkaline pH in alkaliphiles remains challenging. Here, we synthesized for the first time, a thermally activated delayed fluorescent (TADF) pH probe: NI-D-OH. Our probe exhibits a good linear relationship between fluorescence intensity and pH in the neutral to alkaline range (pH 7.0-8.6), as well as long-lived TADF emission. We further show that NI-D-OH can be used to monitor intracellular pH in living organisms, and evaluate the effect of Na+ on the pH homeostasis, demonstrating the potential for alkaline pH monitoring and time-resolved fluorescence imaging.
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