Article
Immunology
Julieta Alcain, Alejandra del Pilar Infante Cruz, Gabriela Barrientos, Silvia Vanzulli, Gabriela Salamone, Monica Vermeulen
Summary: The study demonstrates that histamine can interact with H3R and H4R to promote allergic symptoms and inhibit inflammatory response, leading to an increased proportion of regulatory T lymphocytes and myeloid suppressor cells, which play important roles in the development of allergic contact dermatitis.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Medicine, General & Internal
Keita Yamashita, Shiori Takebayashi, Wataru Murata, Nao Hirai, Yui Ito, Mayuka Mitsui, Mina Saito, Kei Sato, Miyuki Terada, Noriyasu Niizeki, Akira Suzuki, Kenya Ogitani, Toshihiko Fujikawa, Marie Komori, Nozomi Inoue, Norimitsu Arai, Masato Maekawa
Summary: The analytical performance of a novel latex turbidimetric immunoassay method, Nanopia TARC, was evaluated on residual serum samples from patients with skin or allergic diseases.
Article
Immunology
Chunying Xiao, Zhenlai Zhu, Chen Zhang, Jixin Gao, Yixin Luo, Hui Fang, Hongjiang Qiao, Wei Li, Gang Wang, Meng Fu
Summary: This study revealed that newly infiltrated inflammatory dermal Langerin(+) DCs play an essential role in the development of atopic dermatitis (AD) and the local production of TSLP, while TSLP further promotes the production of BM-derived i-Langerin(+) dDCs, which may contribute to the maintenance of AD inflammation.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Nhung Quynh Do, Shengdao Zheng, Sarang Oh, Quynh T. N. Nguyen, Minzhe Fang, Minseon Kim, Junhui Choi, Myeong-Ju Kim, Jeehaeng Jeong, Tae-Hoo Yi
Summary: The study found that camu-camu fruit extract has anti-allergic effects by regulating multiple signaling pathways and transcription factors. This provides new insights into the development of natural products for allergic disorder treatment.
Article
Cardiac & Cardiovascular Systems
Guoshuai Feng, Geetika Bajpai, Pan Ma, Andrew Koenig, Andrea Bredemeyer, Inessa Lokshina, Lulu Lai, Irmgard Foerster, Florian Leuschner, Daniel Kreisel, Kory J. Lavine
Summary: This study identified CCL17 as a proinflammatory mediator in CCR2(+) macrophages and dendritic cells, and suggested that inhibiting CCL17 could promote Treg recruitment and suppress myocardial inflammation.
Article
Chemistry, Multidisciplinary
Zheng Liu, Zhan Fan, Jinxin Liu, Jialu Wang, Mengli Xu, Xinlin Li, Yilun Xu, Yafang Lu, Chenlu Han, Zhihong Zhang
Summary: An immunomodulatory approach based on lipid nanoparticles carrying alpha-helical configurational melittin is developed to treat allergic contact dermatitis and atopic dermatitis by suppressing T cell-mediated inflammatory reactions.
Article
Oncology
Yongjian Dong, Shuhui Gao, Xuefang Zhang, Jing Kou, Jing Liu, Ting Ye, Han Shen
Summary: Recent studies have shown that dendritic cell-activated CIK (DC-CIK) cells exhibit enhanced antitumor activity compared to unstimulated CIK cells. It has been found that dendritic cells can secrete CCL17 and CCL22 to increase the expression of CCR4 on CIK cells, thereby enhancing their migratory capacity and tumor-killing efficacy.
Article
Allergy
ZhengWang Sun, Ji Hye Kim, Seo Hyeong Kim, Hye Ran Kim, KeLun Zhang, Youdong Pan, Min Kyung Ko, Bo Mi Kim, Howard Chu, Hee Ra Lee, Hye Li Kim, Ji Hyung Kim, Xiujun Fu, Young-Min Hyun, Ki Na Yun, Jin Young Kim, Dong Won Lee, Seung Yong Song, Charles P. Lin, Rachael A. Clark, Kwang Hoon Lee, Thomas S. Kupper, Chang Ook Park
Summary: The study found that CXCR4 and its ligand CXCL12 were significantly upregulated in the skin of humans with AD through global transcriptomic and proteomic analyses. Skin-resident CXCR4(+) NKT cells were enriched and consistently elevated in models of mice with AD, suggesting their potential role in contributing to AD pathogenesis.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2021)
Article
Medicine, General & Internal
Chul Hwan Bang, Ji Young Song, Yu Mee Song, Ji Hyun Lee, Young Min Park, Jun Young Lee
Summary: The study revealed that CD45RO(+)CLA(+)H4R(+) T cells are an important source of IL-31 in AD patients. Stimulation with 4-methylhistamine increased IL-31 production in these cells. This suggests that these cells could be a potential target for treating IL-31-induced pruritus.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Immunology
Anna B. Erazo, Nancy Wang, Lena Standke, Adrian D. Semeniuk, Lorenz Fuelle, Sevgi C. Cengiz, Manja Thiem, Heike Weighardt, Richard A. Strugnell, Irmgard Foerster
Summary: This study found that CCL17-expressing DCs were more easily infected by Salmonella in the intestine compared to other DCs. However, the production of CCL17 was not essential for the early dissemination of Salmonella from the gut to systemic organs.
IMMUNITY INFLAMMATION AND DISEASE
(2021)
Article
Nutrition & Dietetics
Boguen Kwon, Soo Yeon Hong, Eun-Young Kim, Jae-Hyun Kim, Minsun Kim, Jae Ho Park, Youngjoo Sohn, Hyuk-Sang Jung
Summary: Cone of Pinus densiflora (CP) has various effects such as anti-oxidation, anti-fungal, anti-bacterial, and anti-melanogenic. Studies have shown that CP has anti-inflammatory effects on allergic contact dermatitis both in vitro and in vivo, suggesting its potential as an alternative medicine for chronic skin inflammation.
Article
Biochemistry & Molecular Biology
Sohshi Morimura, Makoto Sugaya, Tomonori Oka, Hiraku Suga, Tomomitsu Miyagaki, Yuichiro Tsunemi, Yoshihide Asano, Shinichi Sato
Summary: Research using CCL17 TG mice showed that CCL17 can weaken tumor immunity by increasing Tregs and Th2 cells, while decreasing MDSCs, thus reducing the risk of malignant tumors in a Th2-dominant environment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Melisa B. Nicoud, Monica A. Taquez Delgado, Maria de la Paz Sarasola, Agustina Vidal, Daniela Speisky, Graciela A. Cremaschi, Helena A. Sterle, Vanina A. Medina
Summary: Expression of the histamine H4 receptor in immune cells highlights its potential as a therapeutic target for inflammatory and autoimmune diseases. In a breast cancer model, H4R deficiency led to changes in T cell distribution and tumor growth, suggesting the potential for H4R as an immunotherapeutic target.
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2021)
Article
Biochemistry & Molecular Biology
Kuan-Ming Chiu, Yen-Ling Hung, Su-Jane Wang, Yi-Ju Tsai, Nan-Lin Wu, Cher-Wei Liang, Der-Chen Chang, Chi-Feng Hung
Summary: Neferine has anti-allergic and anti-inflammatory effects on mast cells by inhibiting degranulation and cytokine expression, decreasing intracellular calcium levels, and reducing the phosphorylation of the MAPK/NF-kappa B pathway. In vivo studies demonstrate that neferine improves dermatitis appearance, mast cell infiltration, and restores barrier protein expression. Neferine also reduces scratching behavior induced by compound 48/80, suggesting its potential as a natural product for skin allergy and inflammation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Latiffa Amniai, Coline Ple, Mathieu Barrier, Patricia de Nadai, Philippe Marquillies, Han Vorng, Cecile Chenivesse, Anne Tsicopoulos, Catherine Duez
Summary: The study found that CCL18 can induce migration and cytotoxicity of NK cells from nonallergic donors, but had no effect on NK cells from allergic patients. The defect in CCL18 response by NK cells from allergic patients was not due to a binding issue between CCL18 and NK cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Amina Moutayakine, Carolina Marques, Oscar Lopez, Donatella Bagetta, Luisa Leitzbach, Stefanie Hagenow, Elisabete P. Carreiro, Holger Stark, Stefano Alcaro, Jose G. Fernandez-Bolanos, Anthony J. Burke
Summary: A new gem-dimethylchroman-4-ol family of compounds was developed in this study, showing good inhibition of equine serum butyrylcholinesterase (eqBuChE), making them suitable as inhibitors for BuChE.
BIOORGANIC & MEDICINAL CHEMISTRY
(2022)
Article
Chemistry, Medicinal
Nermin Eissa, Karthikkumar Venkatachalam, Petrilla Jayaprakash, Priya Yuvaraju, Markus Falkenstein, Holger Stark, Bassem Sadek
Summary: The study demonstrates the significant mitigating effects of a multiple-active H3R/D2R/D3R antagonist on social deficits related to ASD.
Article
Biochemistry & Molecular Biology
Leonie Beyer, Aylin Sara Kabatas, Susanne Mommert, Holger Stark, Thomas Werfel, Ralf Gutzmer, Katrin Schaper-Gerhardt
Summary: Histamine plays a crucial role in upregulating the IL-18/IL-18R axis and activating human eosinophils from atopic dermatitis patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Neurosciences
Olga A. Sergeeva, Karolina Mazur, David Reiner-Link, Kiril Lutsenko, Helmut L. Haas, Mercedes Alfonso-Prieto, Holger Stark
Summary: Researchers have found that Nα-oleoylhistamine (OLHA) can modulate the activity of histaminergic (HA) neurons in mice. OLHA has different effects on HA neuron firing depending on its concentration. The excitatory and rise in intracellular calcium caused by OLHA are influenced by specific receptors and inhibitors, possibly related to fatty acid metabolism and the PKA pathway. PPAR-alpha and H3 receptors play important roles in this process.
Article
Biochemistry & Molecular Biology
Cecilia M. S. Q. Aranha, David Reiner-Link, Luisa R. Leitzbach, Flavia B. Lopes, Holger Stark, Joao Paulo S. Fernandes
Summary: A series of 21 substituted aryl-alkyl-piperazines were designed, prepared, and tested for their binding affinities at histamine H3 and dopamine D3 receptors, as well as acetyl- and butyrylcholinesterases, in order to generate a potential treatment profile for cognitive impairment.
BIOORGANIC & MEDICINAL CHEMISTRY
(2023)
Article
Pharmacology & Pharmacy
Ozge Uluckan, Sandro Bruno, Yichen Wang, Nathalie Wack, Jenny Wilzopolski, Jean-Francois Goetschy, Corinne Delucis-Bronn, Beatrice Urban, Dominique Fehlmann, Holger Stark, Alice Hauchard, Elsa Roussel, Dominique Kempf, Klemens Kaupmann, Friedrich Raulf, Wolfgang Baeumer, Till A. Rohn, Hans Gunter Zerwes
Summary: In this study, we investigated the pharmacology of adriforant in primary murine cells and in vivo models. Our results showed that adriforant functions as a competitive antagonist of murine histamine receptor 4, reducing itch and inflammation in mice. However, the clinical trial with adriforant did not demonstrate efficacy, suggesting that targeting histamine receptor 4 alone may not be sufficient to treat AD patients.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Burkhard Kleuser, Wolfgang Baeumer
Summary: Sphingolipids are crucial molecules in the mammalian epidermis, playing important roles in the structure of the epidermal layer and regulation of immune cells. The sphingolipid sphingosine 1-phosphate (S1P) is involved in skin cell proliferation and differentiation, and dysregulation of its signaling pathways is associated with inflammatory skin diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Nermin Eissa, Mohamed Al Awad, Shilu Deepa Thomas, Karthikkumar Venkatachalam, Petrilla Jayaprakash, Sicheng Zhong, Holger Stark, Bassem Sadek
Summary: Dysregulation in neurotransmitters and the MAPK pathway is associated with neuropsychiatric disorders like autism spectrum disorder (ASD). Chronic administration of the antagonist ST-713 at the H3R and D2/D3R reduced repetitive self-grooming and aggression in BTBR mice. These effects were reversed by co-administration of H3R agonist or anticholinergic drug. ST-713 also suppressed MAPK pathway proteins and proinflammatory markers, while increasing histamine and dopamine levels in hippocampal tissue. These findings suggest the potential of multiple-targeting compounds like ST-713 in neurodevelopmental disorders.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Justyna Godyn, Paula Zareba, Dorota Stary, Maria Kaleta, Kamil J. Kuder, Gniewomir Latacz, Szczepan Mogilski, David Reiner-Link, Annika Frank, Agata Doroz-Plonka, Agnieszka Olejarz-Maciej, Sylwia Sudol-Talaj, Tobias Nolte, Jadwiga Handzlik, Holger Stark, Anna Wieckowska, Barbara Malawska, Katarzyna Kiec-Kononowicz, Dorota Lazewska, Marek Bajda
Summary: The study designed and synthesized a novel series of benzophenone derivatives. Among them, compound 6 showed high affinity for H3R and significant inhibitory activity towards BuChE. In vitro studies demonstrated that compound 6 had moderate metabolic stability and good permeability. In vivo activity tests revealed that compound 6 exhibited analgesic properties in acute and inflammatory pain.
Article
Cell Biology
Kerstin Griess, Michael Rieck, Nadine Muller, Gergely Karsai, Sonja Hartwig, Angela Pelligra, Robert Hardt, Caroline Schlegel, Jennifer Kuboth, Celina Uhlemeyer, Sandra Trenkamp, Kay Jeruschke, Jurgen Weiss, Leon Peifer-Weiss, Weiwei Xu, Sandra Cames, Xiaoyan Yi, Miriam Cnop, Mathias Beller, Holger Stark, Arun Kumar Kondadi, Andreas S. Reichert, Daniel Markgraf, Marianne Wammers, Dieter Haeussinger, Oliver Kuss, Stefan Lehr, Decio Eizirik, Heiko Lickert, Eckhard Lammert, Michael Roden, Dominic Winter, Hadi Al-Hasani, Doris Hoeglinger, Thorsten Hornemann, Jens C. Bruning, Bengt-Frederik Belgardt
Summary: Impaired proinsulin-to-insulin processing in pancreatic beta-cells is a key defective step in both type 1 diabetes and type 2 diabetes. This study reveals the roles of specific sphingolipid species and sphingolipid-binding proteins in beta-cell function and T2D-associated beta-cell failure.
NATURE CELL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Donia E. Hafez, Mariam Dubiel, Gabriella La Spada, Marco Catto, David Reiner-Link, Yu-Ting Syu, Mohammad Abdel-Halim, Tsong-Long Hwang, Holger Stark, Ashraf H. Abadi
Summary: Introduced a multi-targeted ligand for neurodegenerative diseases, such as Alzheimer's disease, which offers an improved therapeutic alternative compared to the traditional one-target, one-molecule approach. Certain compounds showed potential for multi-targeting by evaluating their interactions with AChE, BuChE, and MAO-B, suggesting their potential as lead structures for developing new multi-targeting anti-AD agents.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Dorota Lazewska, Maria Kaleta, Paula Zareba, Justyna Godyn, Mariam Dubiel, Ewelina Honkisz-Orzechowska, Agata Doroz-Plonka, Anna Wieckowska, Holger Stark, Katarzyna Kiec-Kononowicz
Summary: The aim of the study was to find new multitargeting ligands. Acetyl- and propionyl-phenoxy-pentyl(-hexyl) derivatives were designed and tested for their affinity to human H(3) receptors, inhibition of cholinesterases and monoamine oxidase B, as well as cell toxicity. Compounds 16 and 17 showed promising results with high affinity for H(3) receptors, good inhibition of cholinesterases, and no cell toxicity up to 50 μM.
Article
Multidisciplinary Sciences
Nora Langreder, Dorina Schaeckermann, Doris Meier, Marlies Becker, Maren Schubert, Stefan Duebel, Thomas Reinard, Stefanie Figge-Wegener, Kristine Rossbach, Wolfgang Baeumer, Simone Ladel, Michael Hust
Summary: Insect bite hypersensitivity (IBH), the most common allergic skin disease in horses, is caused by bites from Culicoides spp. insects, resulting in a type I/IVb allergy involving eosinophil cells. A potential treatment option is the use of a therapeutic antibody targeting equine interleukin 5, the main activator and regulator of eosinophils. Through phage display and in vitro affinity maturation, an antibody named NOL226-2-D10 was developed, showing strong inhibition of interleukin 5 binding and satisfactory producibility. This antibody is a promising candidate for in vivo studies to treat equine IBH.
SCIENTIFIC REPORTS
(2023)
Article
Infectious Diseases
Marianne Moerer, Antina Luebke-Becker, Astrid Bethe, Roswitha Merle, Wolfgang Baeumer
Summary: The aim of this study was to investigate the impact of the second amendment to the Regulation of Veterinary Pharmacies (TaHAV) in 2018 on the occurrence of antimicrobial resistance in selected bacterial pathogens isolated from dogs and cats in Germany. The study found that the amendment led to an increase in sample submissions in Germany. The highest resistance rates were found for Staphylococcus pseudintermedius, Staphylococcus aureus, and Escherichia coli, while resistance rates were low for Pasteurella multocida and β-hemolytic streptococci. Significant decreases in resistance rates were observed for S. pseudintermedius against penicillin G and ampicillin, S. felis against amoxicillin-clavulanic acid and cefovecin, and S. aureus against enrofloxacin.