期刊
MOLECULES
卷 27, 期 15, 页码 -出版社
MDPI
DOI: 10.3390/molecules27154693
关键词
chelerythrine; ethoxychelerythrine; fluorescence; absorbance; structural transformation
资金
- National Natural Science Foundation of China [81173496]
- Natural Science Foundation of Hebei Province, China [17273003]
- Science and Technology Research Project of Hebei Province [ZD2019033, Z2020138]
- Doctoral fund of Hebei College of Industry and Technology [BZ201701, BZ2020004]
- Hebei Province 333 Talents Project Funded project [A202101097]
This study compares the fluorescence and absorption spectra of chelerythrine and ethoxychelerythrine in different solvents, and examines their structural changes. The results show that they can substitute each other in the same solvent and exhibit different fluorescence emission wavelengths under different solution conditions.
Chelerythrine (CH) and ethoxychelerythrine (ECH) are chemical reference substances for quality control of Chinese herbal medicines, and ECH is the dihydrogen derivative of CH. In this study, their fluorescence and absorption spectra, as well as their structural changes in different protic solvents were compared. It was observed that their emission fluorescence spectra in methanol were almost the same (both emitted at 400 nm), which may be attributed to the nucleophilic and exchange reactions of CH and ECH with methanol molecules with the common product of 6-methoxy-5,6-dihydrochelerythrine (MCH). When diluted with water, MCH was converted into CH, which mainly existed in the form of positively charged CH+ under acidic and near-neutral conditions with the fluorescence emission at 550 nm. With the increase of pH value of the aqueous solution, CH+ converted to 6-hydroxy-5,6-dihydrochelerythrine (CHOH) with the fluorescence emission at 410 nm. The fluorescence quantum yields of MCH and CHOH were 0.13 and 0.15, respectively, and both the fluorescence intensities were much stronger than that of CH+. It is concluded that CH and ECH can substitute each other in the same protic solvent, which was further verified by high-performance liquid chromatography. This study will help in the investigation of structural changes of benzophenanthridine alkaloids and will provide the possibility for the mutual substitution of standard substances in relevant drug testing.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据