Broadening prime editing toolkits using RNA-Pol-II-driven engineered pegRNA

PrimeDesign software for rapid and simplified design of prime editing guide RNAs

(2021) Jonathan Y. Hsu et al.   Nature Communications

Genome-wide specificity of prime editors in plants

(2021) Shuai Jin et al.   NATURE BIOTECHNOLOGY

Improved prime editors enable pathogenic allele correction and cancer modelling in adult mice

(2021) Pengpeng Liu et al.   Nature Communications

Enhancing prime editing by Csy4-mediated processing of pegRNA

(2021) Yao Liu et al.   CELL RESEARCH

Dual-AAV delivering split prime editor system for in vivo genome editing

(2021) Shengyao Zhi et al.   MOLECULAR THERAPY

Evaluating CRISPR-based prime editing for cancer modeling and CFTR repair in organoids

(2021) Maarten H Geurts et al.   Life Science Alliance

Enhanced prime editing systems by manipulating cellular determinants of editing outcomes

(2021) Peter J. Chen et al.   CELL

Engineered pegRNAs improve prime editing efficiency

(2021) James W. Nelson et al.   NATURE BIOTECHNOLOGY

Comprehensive analysis of prime editing outcomes in human embryonic stem cells

(2021) Omer Habib et al.   NUCLEIC ACIDS RESEARCH

A systematic genome-wide mapping of oncogenic mutation selection during CRISPR-Cas9 genome editing

(2021) Sanju Sinha et al.   Nature Communications

Blackjack mutations improve the on-target activities of increased fidelity variants of SpCas9 with 5′G-extended sgRNAs

(2020) Péter István Kulcsár et al.   Nature Communications

Prime genome editing in rice and wheat

(2020) Qiupeng Lin et al.   NATURE BIOTECHNOLOGY

Cas9 activates the p53 pathway and selects for p53-inactivating mutations

(2020) Oana M. Enache et al.   NATURE GENETICS

Efficient generation of mouse models with the prime editing system

(2020) Yao Liu et al.   Cell Discovery

CRISPR/Cas9 treatment causes extended TP53-dependent cell cycle arrest in human cells

(2020) Jonathan M Geisinger et al.   NUCLEIC ACIDS RESEARCH

Unbiased investigation of specificities of prime editing systems in human cells

(2020) Do Yon Kim et al.   NUCLEIC ACIDS RESEARCH

Prime editing for functional repair in patient-derived disease models

(2020) Imre F. Schene et al.   Nature Communications

Decoupling tRNA promoter and processing activities enables specific Pol-II Cas9 guide RNA expression

(2019) David J. H. F. Knapp et al.   Nature Communications

Oligogenic inheritance of a human heart disease involving a genetic modifier

(2019) Casey A. Gifford et al.   SCIENCE

Targeted deletion of floral development genes in Arabidopsis with CRISPR/Cas9 using the RNA endoribonuclease Csy4 processing system

(2019) Yingzhu Liu et al.   Horticulture Research

Search-and-replace genome editing without double-strand breaks or donor DNA

(2019) Andrew V. Anzalone et al.   NATURE

p53 inhibits CRISPR–Cas9 engineering in human pluripotent stem cells

(2018) Robert J. Ihry et al.   NATURE MEDICINE

CRISPR–Cas9 genome editing induces a p53-mediated DNA damage response

(2018) Emma Haapaniemi et al.   NATURE MEDICINE

Augmenting CRISPR applications in Drosophila with tRNA-flanked sgRNAs

(2016) Fillip Port et al.   NATURE METHODS

Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases

(2014) S. Bae et al.   BIOINFORMATICS

Multiplexed and Programmable Regulation of Gene Networks with an Integrated RNA and CRISPR/Cas Toolkit in Human Cells

(2014) Lior Nissim et al.   MOLECULAR CELL

Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing

(2014) Shengdar Q Tsai et al.   NATURE BIOTECHNOLOGY

Self-processing of ribozyme-flanked RNAs into guide RNAsin vitroandin vivofor CRISPR-mediated genome editing

(2013) Yangbin Gao et al.   Journal of Integrative Plant Biology

Large T Antigens of Polyomaviruses: Amazing Molecular Machines

(2012) Ping An et al.   Annual Review of Microbiology

A more efficient method to generate integration-free human iPS cells

(2011) Keisuke Okita et al.   NATURE METHODS