Article
Radiology, Nuclear Medicine & Medical Imaging
Kyung Min Nam, Ayhan Gursan, Alex A. Bhogal, Jannie P. Wijnen, Dennis W. J. Klomp, Jeanine J. Prompers, Arjan D. Hendriks
Summary: This study demonstrates the feasibility of accelerating 3D deuterium metabolic imaging in the human liver using deuterium echo-planar spectroscopic imaging (EPSI) at 7 T. The results show that deuterium EPSI has slightly lower signal-to-noise ratio (SNR) compared to conventional MRSI, but it significantly reduces the acquisition time. By using deuterium EPSI, it is possible to monitor hepatic glucose dynamics with full liver coverage, high spatial and temporal resolution, and the acquisition time can be further shortened.
MAGNETIC RESONANCE IN MEDICINE
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Henk M. De Feyter, Monique A. Thomas, Kevin L. Behar, Robin A. de Graaf
Summary: The study found that H-2-labeled glycogen was barely detectable in solution using H-2 NMR due to its very short T-2 (<2 ms), resulting in a spectral line width that is more than five times as broad as that of C-13-labeled glycogen. This indicates that H-2-labeled glycogen is not detectable with 2H MRS under in vivo conditions, making C-13 MRS the preferred technique for in vivo detection of glycogen.
MAGNETIC RESONANCE IN MEDICINE
(2021)
Article
Chemistry, Analytical
Ke Chen, Yuhui Xiang, Xiaoyu Yan, Zhenghui Li, Rui Qin, Jie Sun
Summary: Stable isotope-assisted metabolomics (SIAM) allows global tracking of isotopic labels in living organisms, but its application in tracking transformation products (TPs) of environmental contaminants is challenging. In this study, a H-2-SIAM pipeline coupled with a flexible algorithm was developed to track TPs of contaminants in the environmental matrix.
ANALYTICAL CHEMISTRY
(2022)
Article
Biophysics
Friederike Hesse, Alan Wright, Flaviu Bulat, Felix Kreis, Kevin M. Brindle
Summary: The sensitivity of a metabolism imaging technique for detecting tumor cell death was assessed by lowering the concentration of injected [2,3-H-2(2)]fumarate and varying the extent of cell death through changes in drug concentration. The results showed that the production of [2,3-H-2(2)]malate plateaued at a tumor fumarate concentration of 2 mM, and the tumor malate concentration and malate/fumarate ratio increased linearly with the extent of cell death determined histologically.
NMR IN BIOMEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Francesca Ferrara, Mascia Benedusi, Franco Cervellati, Maddalena Sguizzato, Leda Montesi, Agnese Bondi, Markus Drechsler, Walter Pula, Giuseppe Valacchi, Elisabetta Esposito
Summary: In this study, transethosomes were explored as potential delivery systems for dimethyl fumarate. Formulative studies were conducted to investigate the effects of various compositions on the morphology and drug entrapment capacity of vesicles. Results showed that certain formulations were able to effectively improve wound healing and control drug release, indicating their potential application in wound treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Clinical Neurology
Thomas W. Lategan, Laurene Wang, Tiffany N. Sprague, Franck S. Rousseau
Summary: The study confirmed the bioequivalence between two Bafiertam(TM) capsules and one Tecfidera(R) capsule, both of which were found to be safe and generally well tolerated.
Article
Chemistry, Multidisciplinary
Annalisa Rosso, Valentina Andretto, Yves Chevalier, David Kryza, Jacqueline Sidi-Boumedine, Ana Grenha, Filipa Guerreiro, Adem Gharsallaoui, Veronica La Padula, Alexandra Montembault, Laurent David, Stephanie Briancon, Giovanna Lollo
Summary: This work proposes a novel strategy to increase drug residence time in the intestine by developing nanocomposites combining mucopenetrating and mucoadhesive properties. The nanocomposites were successfully prepared and their mucopenetration ability and stability were characterized. In vitro cell culture experiments demonstrated the cytocompatibility of the nanocomposite system, while in vivo biodistribution studies in mice showed enhanced intestinal retention of the nanocomposite compared to the nanosystem suspension.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Clinical Neurology
Franck S. Rousseau, Laurene Wang, Tiffany N. Sprague, Thomas W. Lategan, Regina R. Berkovich
Summary: This study evaluated the pharmacokinetics and relative bioavailability of two FDA-approved drugs, Bafiertam and Vumerity, for the treatment of multiple sclerosis. The results showed that there were slightly higher mean maximum observed concentration and area under the curve for Bafiertam compared to Vumerity, but the difference was not significant. These findings provide some guidance for the selection of appropriate fumarate products.
MULTIPLE SCLEROSIS AND RELATED DISORDERS
(2023)
Article
Chemistry, Multidisciplinary
Farah Benyettou, Nawel Kaddour, Thirumurugan Prakasam, Gobinda Das, Sudhir Kumar Sharma, Sneha Ann Thomas, Fadia Bekhti-Sari, Jamie Whelan, Mohammed A. Alkhalifah, Mostafa Khair, Hassan Traboulsi, Renu Pasricha, Ramesh Jagannathan, Nassima Mokhtari-Soulimane, Felipe Gandara, Ali Trabolsi
Summary: This study introduces a novel approach using nCOF nanoparticles for oral insulin delivery, which demonstrated protection of insulin and glucose-responsive release in vitro, as well as hyperglycemia-induced release in vivo without toxic effects in diabetic rats. These findings suggest that nCOF-based oral insulin delivery systems could potentially replace traditional subcutaneous injections for insulin therapy.
Article
Multidisciplinary Sciences
Abhinav Kaushik, Diane Dunham, Xiaorui Han, Evan Do, Sandra Andorf, Sheena Gupta, Andrea Fernandes, Laurie Elizabeth Kost, Sayantani B. Sindher, Wong Yu, Mindy Tsai, Robert Tibshirani, Scott D. Boyd, Manisha Desai, Holden T. Maecker, Stephen J. Galli, R. Sharon Chinthrajah, Rosemarie H. DeKruyff, Monali Manohar, Kari C. Nadeau
Summary: This study analyzed immune cells from participants in a peanut oral immunotherapy (OIT) trial and found that the differentiation status of CD8(+) T cells at baseline may predict the likelihood of achieving sustained unresponsiveness (SU). The study also found that lower frequencies of naïve CD8(+) T cells and terminally differentiated CD57(+)CD8(+) T cell subsets at baseline were associated with SU, while higher frequencies of IL-4(+) and IFN gamma(+) CD4(+) T cells post-OIT were correlated with a lower likelihood of SU.
NATURE COMMUNICATIONS
(2022)
Article
Biochemical Research Methods
Vladimir Kanygin, Alexander Zaboronok, Iuliia Taskaeva, Evgenii Zavjalov, Rinat Mukhamadiyarov, Aleksandr Kichigin, Anna Kasatova, Ivan Razumov, Roman Sibirtsev, Bryan J. Mathis
Summary: This study investigated the accumulation and biodistribution of pegylated liposomes with encapsulated borocaptate and a fluorescent label in different types of cancer cells, showing higher accumulation in tumors compared to normal cells with characteristic concentration peaks in certain cell lines, and providing in vivo tumor selectivity with several-fold higher tumor tissue fluorescence at the 6-hour time point.
JOURNAL OF FLUORESCENCE
(2021)
Article
Oncology
Flaviu Bulat, Friederike Hesse, Bala Attili, Chandra Solanki, Iosif A. Mendichovszky, Franklin Aigbirhio, Finian J. Leeper, Kevin M. Brindle, Andre A. Neves
Summary: There is a clinical demand for imaging agents that can detect tumor cell death after treatment. We present a gallium-68-labeled derivative of Synaptotagmin-I for imaging tumor cell death using PET. Ga-68-C2Am could be a valuable tool for rapidly assessing tumor responses to treatment.
Article
Cell Biology
Sean Mccracken, Michael J. Fitzpatrick, Allison L. Hall, Zelun Wang, Daniel Kerschensteiner, Josh L. Morgan, Philip R. Williams
Summary: Retinal ganglion cell (RGC) degeneration, caused by axon degeneration, is the main reason for vision loss in blinding conditions. Axonal Ca2+ elevations from optic nerve injury do not reach RGC somas, and baseline Ca2+ levels of RGCs predict their survival after axon injury.
Article
Pharmacology & Pharmacy
Richard Wibel, Arne Matteo Joergensen, Flavia Laffleur, Helen Spleis, Victor Claus, Andreas Bernkop-Schnuerch
Summary: Hydrophobic ion pairing and incorporation into SEDDS is a promising strategy for oral delivery of hydrophilic macromolecular drugs. In this study, hydrophobic ion pairs (HIP) between sCT and highly lipophilic sulfosuccinate counterions were formed. The use of bis(isotridecyl) sulfosuccinate resulted in the highest lipophilicity and improved solubility in lipophilic co-solvents. The HIP-loaded SEDDS showed excellent pharmacological activity in rats, with a relative hypocalcaemic effect of 13.8 +/- 5.6%.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Medicine, Research & Experimental
Yuan Cheng, Dai Shi, Zhan Xu, Zhihui Gao, Zhan Si, Yanzhao Zhao, Renjie Ye, Zhequan Fu, Wenhui Fu, Tingting Yang, Yan Xiu, Qingyu Lin, Dengfeng Cheng
Summary: Lung cancer, divided into small and nonsmall cell lung cancer, is a highly heterogeneous cancer. In this study, the F(ab ')(2) fragments of Durva were used to evaluate PD-L1 expression, and I-124-Durva-F(ab ')(2) was identified as a promising PD-L1 immunoPET tracer.
MOLECULAR PHARMACEUTICS
(2022)
Article
Radiology, Nuclear Medicine & Medical Imaging
Vencel Somai, Felix Kreis, Adam Gaunt, Anastasia Tsyben, Ming Li Chia, Friederike Hesse, Alan J. Wright, Kevin M. Brindle
Summary: The method described in the study utilizes a stochastic numerical solver for optimizing pulse sequences, providing a simple framework for incorporating constraints and complex cost functions. Experimental results show improved signal-to-noise ratio with optimized polarization transfer pulses and achieve J-coupling modulation-free excitation.
MAGNETIC RESONANCE IN MEDICINE
(2022)
Article
Chemistry, Medicinal
Cristina Barca, Christoph M. Griessinger, Andreas Faust, Dominic Depke, Markus Essler, Albert D. Windhorst, Nick Devoogdt, Kevin M. Brindle, Michael Schaefers, Bastian Zinnhardt, Andreas H. Jacobs
Summary: Radioligand theranostics in oncology utilize cancer-type specific biomarkers and molecular imaging for patient diagnosis, therapy, and personalized management. The concept has evolved with the development of theranostic pairs and the combination of nuclear medicine with different types of cancer therapies. The clinical applications of different theranostic radiopharmaceuticals in managing different tumor types support the combination of innovative oncological therapies such as gene and cell-based therapies with RT.
Review
Multidisciplinary Sciences
David Crosby, Sangeeta Bhatia, Kevin M. Brindle, Lisa M. Coussens, Caroline Dive, Mark Emberton, Sadik Esener, Rebecca C. Fitzgerald, Sanjiv S. Gambhir, Peter Kuhn, Timothy R. Rebbeck, Shankar Balasubramanian
Summary: Early detection of cancer is crucial for improving survival rates, but unfortunately, a significant number of cases are diagnosed at an advanced stage. Overcoming various challenges is essential to achieve early detection for all cancers, including understanding high-risk individuals, elucidating the biology and trajectory of precancer and early cancer, and developing sensitive and specific detection technologies.
Article
Oncology
Friederike Hesse, Alan J. Wright, Vencel Somai, Flaviu Bulat, Felix Kreis, Kevin M. Brindle
Summary: The study demonstrates that detecting fumarate metabolism using H-2 MRI can early identify tumor cell death in glioblastoma following chemoradiation, potentially improving the clinical evaluation of treatment response.
Article
Biophysics
Friederike Hesse, Alan Wright, Flaviu Bulat, Felix Kreis, Kevin M. Brindle
Summary: The sensitivity of a metabolism imaging technique for detecting tumor cell death was assessed by lowering the concentration of injected [2,3-H-2(2)]fumarate and varying the extent of cell death through changes in drug concentration. The results showed that the production of [2,3-H-2(2)]malate plateaued at a tumor fumarate concentration of 2 mM, and the tumor malate concentration and malate/fumarate ratio increased linearly with the extent of cell death determined histologically.
NMR IN BIOMEDICINE
(2023)
Review
Chemistry, Physical
Jacob Chen Ming Low, Alan J. Wright, Friederike Hesse, Jianbo Cao, Kevin M. Brindle
Summary: Deuterium metabolic imaging (DMI) is a clinically-applicable technique for non-invasive investigation of tissue metabolism. It shows potential for imaging tissue metabolism and cell death in vivo. In this study, DMI is compared with established metabolic imaging techniques including PET and 13C MR imaging.
PROGRESS IN NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY
(2023)
Article
Oncology
Flaviu Bulat, Friederike Hesse, Bala Attili, Chandra Solanki, Iosif A. Mendichovszky, Franklin Aigbirhio, Finian J. Leeper, Kevin M. Brindle, Andre A. Neves
Summary: There is a clinical demand for imaging agents that can detect tumor cell death after treatment. We present a gallium-68-labeled derivative of Synaptotagmin-I for imaging tumor cell death using PET. Ga-68-C2Am could be a valuable tool for rapidly assessing tumor responses to treatment.
Article
Oncology
Maria Fala, Susana Ros, Ashley Sawle, Jyotsna U. Rao, Anastasia Tsyben, Laura Tronci, Christian Frezza, Richard Mair, Kevin M. Brindle
Summary: BCAT1 expression in glioblastoma varies widely, and its role in cell proliferation and invasion depends on the tumor subtype.
NEURO-ONCOLOGY ADVANCES
(2023)