Article
Chemistry, Analytical
Xu Chen, Wei Li, Yue Ma
Summary: This study developed a method combining CAMP and PMA for real-time and visual detection of viable Salmonella in milk. The method showed high sensitivity and recovery rate, and can be performed under isothermal conditions.
ANALYTICAL METHODS
(2022)
Article
Chemistry, Analytical
Zhen Yang, Miaoning Ren, Ya Li, Mingxing Zhou, Jingyi Peng, Si Lin, Kang Du, Xian Huang
Summary: Methods to manipulate magnetic beads play a crucial role in determining the efficiency and size of in vitro diagnostic systems. This study proposes a technique to construct a fully integrated microfluidic device that enables automatic magnetic bead manipulation, rapid chemical reactions, and cleaning in a compact dimension similar to a USB disk. The device combines the precision control of multiple electromagnetic coils and the compactness of microfluidic channels, making it one of the smallest automatic magnetic bead manipulation systems.
ANALYTICAL CHEMISTRY
(2023)
Article
Medicine, Research & Experimental
Dafne Berenice Hormazabal, Angeles Beatriz Reyes, Francisco Castro, Alan R. Cabrera, Paulina Dreyse, Felipe Melo-Gonzalez, Susan M. Bueno, Ivan A. Gonzalez, Christian Erick Palavecino
Summary: In this study, the efficacy of photodynamic inactivation (PDI) using Rubased photosensitizers (PSRu-L2 and PSRu-L3) on ESBL-producing Klebsiella pneumoniae was tested. The mode of action was characterized, and the synergistic effect of PSRu-L2 and PSRu-L3 with cefotaxime was evaluated. The results showed that the PSs compounds were bactericidal, inhibited bacterial growth, and had low cytotoxicity in mammalian cells.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Infectious Diseases
Mei-Chen Ou-Yang, Jen-Fu Hsu, Shih-Ming Chu, Ching-Min Chang, Chih-Chen Chen, Hsuan-Rong Huang, Peng-Hong Yang, Ren-Huei Fu, Ming-Horng Tsai
Summary: The use of initial broad-spectrum antibiotics in critically ill neonates requiring mechanical intubation for respiratory failure has not been well studied. This study compared the outcomes of neonates receiving ampicillin plus cefotaxime with those receiving ampicillin plus gentamicin. The use of ampicillin plus cefotaxime was associated with worse outcomes, including a higher risk of infections caused by multidrug-resistant pathogens and increased mortality.
Article
Chemistry, Analytical
Yuting Shang, Qinghua Ye, Qingping Wu, Xinran Xiang, Fei Zha, Mingzhu Du, Jumei Zhang
Summary: This study established a multiplex PCR technique for rapid identification of Salmonella serogroups and serovars. Specific target genes were identified through pan-genome analysis and PCR verification, and novel mPCR assays were developed based on these targets. These mPCR assays showed high specificity and accuracy, and were capable of detecting target Salmonella in food samples.
ANALYTICAL METHODS
(2022)
Article
Microbiology
Haoran Zhang, Ying Xiang, Yong Huang, Beibei Liang, Xuebin Xu, Jing Xie, Xinying Du, Chaojie Yang, Hongbo Liu, Hui Wang, Mingjuan Yang, Ligui Wang, Xiaofeng Hu, Lianqun Jin, Jinsong Li, Yongqiang Jiang, Shaofu Qiu, Hongbin Song
Summary: This study reports on 11 multidrug-resistant Salmonella enterica serovar Typhimurium strains harboring the colistin resistance gene mcr-1 in China. The findings indicate that the mcr-1 gene can effectively spread among intestinal bacteria by horizontal transfer and highlight the importance of constant surveillance of mcr-1 harboring Salmonella strains to prevent the spread of colistin resistance.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Alemayehu Gebeyehu, Mestawet Taye, Rahmeto Abebe
Summary: This study investigated the distribution and antibiotic susceptibility of Salmonella in raw cows' milk from dairy farms and households in Hawassa, Arsi Negele, and Dale districts of Ethiopia. The results showed a detection rate of 10.42% for Salmonella in the collected samples. There was no significant variation in the prevalence of Salmonella between different districts, households, and seasons, but there was a significant difference in the isolation rate between different scales of dairy farms. All isolates exhibited multidrug resistance, with 100% resistance to ampicillin and 100% sensitivity to ciprofloxacin.
Article
Immunology
Yosef Daniel Huberman, Melanie Caballero-Garcia, Rober Rojas, Silvia Ascanio, Leandro Hipolito Olmos, Rosana Malena, Jorgelina Lomonaco, Paula Nievas, Paula Chero, Julio Levano-Gracia, Alfredo Mendoza-Espinoza
Summary: Poultry infections by Salmonella cause significant economic losses worldwide. Poultry serves as a common reservoir of Salmonella, posing a risk to humans through the food chain. Vaccination of layers plays an important role in preventing Salmonella infections.
Article
Environmental Sciences
Thamizhselvan Surya, Geevaretnam Jeyasekaran, Robinson Jeya Shakila, Balasubramanian Sivaraman, Rajendran Shalini, Shanmugam Sundhar, Ulaganathan Arisekar
Summary: The study investigated the prevalence of biofilm-forming Salmonella on different seafood contact surfaces, finding that these Salmonella were more common on boat decks and seafood processing plants. Two out of four isolates showed strong biofilm-forming ability, while the other two showed moderate ability.
MARINE POLLUTION BULLETIN
(2022)
Article
Biochemistry & Molecular Biology
Mariana de Fatima Albuquerque Pereira, Larissa Gabriela Morais de Avila, Bruna Cristina dos Santos Cruz, Bruno Campos Silva, Leandro Licursi de Oliveira, Reggiani Vilela Goncalves, Andrea de Oliveira Barros Ribon, Tiago Antonio de Oliveira Mendes, Maria do Carmo Gouveia Peluzio
Summary: This study evaluated the role of milk kefir on inflammation and gut microbiome in mice infected with Salmonella. The results showed that kefir could prevent systemic infections and regulate cytokines in IL-10(-/-) mice, but not in wild type mice. These findings demonstrate the importance of IL-10 in the prognosis of salmonellosis and the potential use of milk kefir in acute infections.
Article
Microbiology
Alessandra Alessiani, Elisa Goffredo, Maria Mancini, Gilda Occhiochiuso, Simona Faleo, Antonella Didonna, Rita Fischetto, Federica Suglia, Danila De Vito, Antonella Stallone, Luigi D'Attoli, Adelia Donatiello
Summary: Salmonella enterica is a common cause of foodborne infection worldwide, especially in Italy. A study on the antimicrobial resistance of Salmonella in Apulia and Basilicata provides a focused perspective on the region, revealing high resistance to several antibiotics and the need for further research and action in antibiotic management.
Article
Immunology
Liwei Zhao, Jianchang Wang, Xiao Xia Sun, Jinfeng Wang, Zhimin Chen, Xiangdong Xu, Mengyuan Dong, Ya-nan Guo, Yuanyuan Wang, Pingping Chen, Weijuan Gao, Yunyun Geng
Summary: The study utilized a simple, rapid, sensitive, and reliable real-time recombinase polymerase amplification technology to successfully detect Salmonella spp., with analysis time reduced to 5-12 minutes and equally reliable results compared to traditional PCR assays.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Oncology
Tingting Geng, Miao Li, Rong Chen, Shuangyu Yang, Guoquan Jin, Tinabo Jin, Fulin Chen
Summary: This study assessed the correlation between single nucleotide polymorphisms (SNPs) in DNA repair genes and the risk of lung cancer. The results showed that a SNP in the RAD54L2 gene increased the risk of lung cancer, and different SNPs showed associations with lung cancer risk in different age, smoking, and drinking groups. These findings may contribute to a better understanding of the impact of genetic polymorphisms on lung cancer occurrence.
Article
Microbiology
Carlos Ratia, Virginio Cepas, Raquel Soengas, Yolanda Navarro, Maria Velasco-de Andres, Maria Jose Iglesias, Francisco Lozano, Fernando Lopez-Ortiz, Sara M. Soto
Summary: The emergence and spread of infections caused by multidrug-resistant bacteria pose a threat to the effectiveness of current antibiotics. Gold-based drugs, specifically gold(III) complexes, have shown potential as antibacterial agents. In this study, the antibacterial activity of the gold(III) complex 2 was demonstrated, and a synergistic effect was observed when combined with colistin.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
A. Kreitlow, A. Becker, U. Schotte, B. Malorny, M. Ploetz, A. Abdulmawjood
Summary: The LAMP technique was utilized to identify salmonella using six specific sequences as targets, with ttrRSBCA primers showing the best overall performance. Other primer sets had deficiencies, with some failing to detect certain Salmonella serotypes.
LETTERS IN APPLIED MICROBIOLOGY
(2021)
Article
Food Science & Technology
Aya Elsayed Maghrabia, Hend Ali Elshebrawy, Mahmoud Ahmed Mahros, Mohammed Mohammed Elgazzar, Khalid Ibrahim Sallam
Summary: Helicobacter pylori is an important medical pathogen associated with gastric adenocarcinomas and gastric mucosa-associated lymphoid tissue lymphomas. However, its prevalence in food, especially meat and meat products, is not fully understood. This study investigated the prevalence, molecular characterization, and antimicrobial resistance profiles of clarithromycin- and metronidazole-resistant H. pylori isolated from raw and ready-to-eat meat samples in Egypt. The results showed a high prevalence of H. pylori in both raw and ready-to-eat meat products, and a high level of multidrug resistance among the isolates. The findings highlight the potential public health hazard associated with the widespread contamination of meat products with multidrug-resistant H. pylori.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Food Science & Technology
Elham Elsayed Abo-Almagd, Rana Fahmi Sabala, Samir Mohammed Abd-Elghany, Charlene R. Jackson, Hazem Ramadan, Kalman Imre, Adriana Morar, Viorel Herman, Khalid Ibrahim Sallam
Summary: This study investigated the prevalence, antibiotic resistance profiles, phylogroups, and beta-lactamase genes of Escherichia coli isolates from chicken carcasses in Mansoura, Egypt. The results showed a high contamination rate of E. coli in the examined chicken carcasses, with 98% of them testing positive for E. coli. It was found that a majority of the E. coli strains exhibited multidrug resistance and carried beta-lactamase genes.
Article
Food Science & Technology
Khalid Ibrahim Sallam, Yasmine Abd-Elrazik, Mona Talaat Raslan, Kalman Imre, Adriana Morar, Viorel Herman, Hanan Ahmed Zaher
Summary: The study examined the occurrence and antimicrobial resistance profiles of E. coli O157:H7 and O55:H7 in camel meat in Egypt. Out of 110 samples, 9.1% were positive for E. coli O157:H7 and 29.1% were positive for E. coli O55:H7. The isolates showed resistance to multiple antibiotics, highlighting the potential threat to public health. Further studies are recommended to understand the antimicrobial resistance phenomenon in camel-meat-originating E. coli strains.
Article
Food Science & Technology
Reda Abdallah, Nader Y. Y. Mostafa, Ghada A. K. Kirrella, Ibrahim Gaballah, Kalman Imre, Adriana Morar, Viorel Herman, Khalid Ibrahim Sallam, Hend Ali Elshebrawy
Summary: Consumers are increasingly aware of the importance of safe and natural additives in meat products. This study explored the antimicrobial effect of Moringa oleifera leaves extract on ground beef, demonstrating its potential as a healthy and safe preservative that can improve the shelf stability of meat products without compromising sensory attributes. Using natural food additives instead of chemical preservatives could revolutionize the food industry by ensuring safety and quality for consumers.
Article
Biology
Nadir Boudjlal Dergal, Mohamed Ghermi, Kalman Imre, Adriana Morar, Ulas Acaroz, Damla Arslan-Acaroz, Viorel Herman, Abdelhanine Ayad
Summary: This 10-year retrospective survey found that the prevalence of tuberculosis in slaughtered animals in Constantine Province, Algeria was 0.83%. The study suggests that tuberculosis remains a serious public and animal health issue in the screened region, although the incidence is not highly alarming.
Article
Veterinary Sciences
Diana Maria Degi, Kalman Imre, Viorel Herman, Janos Degi, Romeo Teodor Cristina, Adela Marcu, Florica Morariu, Florin Muselin
Summary: The study aimed to evaluate the phytotherapeutic potential of Sempervivum tectorum L. by quantitatively analyzing its biologically active substances. The polyphenol and proanthocyanidin contents of Sempervivum tectorum L. were investigated using modern methods of phytoanalytics, and its antimicrobial activity was tested against pathogenic bacteria. The results showed that the plant extracts exhibited strong antibacterial activity and contained high levels of total phenolics and proanthocyanidin, indicating their contribution to the antimicrobial activity.
VETERINARY SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Sebastian Ciui, Adriana Morar, Emil Tirziu, Viorel Herman, Alexandra Ban-Cucerzan, Sebastian Alexandru Popa, Doru Morar, Mirela Imre, Adrian Olariu-Jurca, Kalman Imre
Summary: This study investigated the causes of carcass and organ condemnations and estimated the financial losses suffered by a cattle slaughterhouse. The results showed that all types of edible parts had abnormalities, with different degrees of severity. The total direct financial losses resulting from edible part condemnation over the two years was estimated at EUR 4,021,717.3, which represents 1.17% of the total achievable net revenue.
Article
Biochemistry & Molecular Biology
Diana Obistioiu, Anca Hulea, Ileana Cocan, Ersilia Alexa, Monica Negrea, Iuliana Popescu, Viorel Herman, Ilinca Merima Imbrea, Gabriel Heghedus-Mindru, Mukhtar Adeiza Suleiman, Isidora Radulov, Florin Imbrea
Summary: The research aims to evaluate the chemical composition, antioxidant and anti-inflammatory activity, as well as antimicrobial activity of a commercial Boswellia essential oil. The findings demonstrate the oil's high antioxidant and antimicrobial potential against food-related pathogens, making it a good candidate for use in food, feed, or food-safety-related products.
Article
Food Science & Technology
Rehab Mohammed El-Mougy, Samir Mohammed Abd-Elghany, Kalman Imre, Adriana Morar, Viorel Herman, Khalid Ibrahim Sallam
Summary: This study developed a HACCP strategy for Egyptian dry-cured pastrami production, identifying all potential hazards and critical control points in each production step. Based on two previous publications, this scientific paper completes the existing knowledge. The HACCP system is specifically applied to the stages from receiving raw materials to packaging and storage, with four critical control points identified and suitable corrective actions arranged. Regular review and accurate record-keeping are necessary for validation, and maintaining a certified supply chain is crucial for food safety.
Article
Infectious Diseases
Alexandra Ban-Cucerzan, Adriana Morar, Emil Tirziu, Kalman Imre
Summary: The aim of this study was to evaluate the hygiene of pork, beef, and poultry carcasses and the antibiotic susceptibility of bacteria in the biofilm on the carcasses. The findings reveal significant sanitary deficiencies in meat processing units, leading to the presence of multidrug-resistant bacteria in meat products, posing a serious public health risk.
Article
Veterinary Sciences
Hanane Derbak, Kalman Imre, Amira Chahrazad Benabdelhak, Mohamed Moussaoui, Amina Kribeche, Rosa Kebbi, Abdelhanine Ayad
Summary: The study aims to evaluate the effects of the total alkaloid extract of Algerian Peganum harmala seeds on sexual behavior and male reproductive function. The results show that the extract acts in a dose-dependent manner and has varying effects on the reproductive parameters of male mice. While some parameters were affected, there was no significant impact on sperm quantity, motility, membrane integrity, and total antioxidant capacity.
VETERINARY SCIENCES
(2023)
Article
Veterinary Sciences
A. Stancu, Adela Marcu, Iasmina Luca, I. Hutu, G. Orghici, O. S. Voia, V. Herman
Summary: A high prevalence of pulmonary lesions and various types of gastroenteritis were found in sheep carcasses examined in three counties in Romania.
REVISTA ROMANA DE MEDICINA VETERINARA
(2023)
