期刊
JOURNAL OF VASCULAR RESEARCH
卷 59, 期 5, 页码 288-302出版社
KARGER
DOI: 10.1159/000526027
关键词
Adipose tissue; Nitric oxide synthase; Obesity; Resistance arteries; Vascular tone
资金
- British Heart Foundation [PG/16/52/32229]
- British Heart Foundation [PG/16/52/32229] Funding Source: researchfish
This study compared the anticontractile function of subcutaneous and visceral perivascular adipose tissue (PVAT) depots and found that their mechanisms are similar. Both endothelial and neuronal NOS isoforms play a role in the anticontractile effect of PVAT. Obesity leads to impaired PVAT anticontractile function, but it is independent of impaired vasoreactivity and can be restored by activating NOS in the visceral PVAT.
Background: The mechanism of the perivascular adipose tissue (PVAT) anticontractile effect is well characterized in rodent visceral vascular beds; however, little is known about the mechanism of PVAT anticontractile function in subcutaneous vessels. In addition, we have previously shown that PVAT anticontractile function is nitric oxide synthase (NOS) dependent but have not investigated the roles of NOS isoforms. Objective: Here, we examined PVAT anticontractile function in the mouse gracilis artery, a subcutaneous fat depot, in lean control and obese mice and investigated the mechanism in comparison to a visceral depot. Method: Using the wire myograph, we generated responses to noradrenaline and electrical field stimulation in the presence of pharmacological tools targeting components of the known PVAT anticontractile mechanism. In addition, we performed ex vivo fat transplants in the organ bath. Results: The mechanism of PVAT anticontractile function is similar between subcutaneous and visceral PVAT depots. Both endothelial and neuronal NOS isoforms mediated the PVAT anticontractile effect. Loss of PVAT anticontractile function in obesity is independent of impaired vasoreactivity, and function can be restored in visceral PVAT by NOS activation. Conclusions: Targeting NOS isoforms may be useful in restoring PVAT anticontractile function in obesity, ameliorating increased vascular tone, and disease. (c) 2022 The Author(s). Published by S. Karger AG, Basel
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