Green synthesis and Characterization of Copper oxide nanoparticles using Calotropis procera leaf extract and their different biological potentials

Plant mediated nano-fabrication is an emerging field of nanotechnology which is preferred over conventional techniques because of its safety, cost effectiveness, ecofriendliness and biocompatibility properties. The current study includes Calotropis procera mediated synthesis of copper oxide nanoparticles (CuONPs) using aqueous plant extract and their anti-pathogenic activities against phytopathogens. Different microscopic and spectroscopic characterization techniques such as X-ray powder diffraction (XRD), Fourier transformed infrared spectroscopy (FTIR), UV-visible spectrophotometer (UV), Scanning electron microscope (SEM) and Transmission electron microscope (TEM) were performed to confirm the biofabrication of Calotropis procera mediated CuONPs. XRD results showed high crystalline nature of the particles ranging from 40 to 100 nm. SEM and TEM images revealed spherical shape and size ranging from 20 to 80 nm. FTIR and UV-visible spectrophotometer confirmed the presence of different functional groups involved in capping, reducing and stabilization of the CuONPs. Moreover, Anti-fungal and anti-bacterial activities were performed which showed maximum inhibition at 100 mu g/mL concentration in both in vitro and in detached leaf assay of Muskmelon. In conclusion, our results of greenly synthesized CuONPs showed significant biological potentials and potent antioxidant activity as a result it can be used in phytopathology against plant pathogens. In future, we suggest different other in vitro and in vivo bioactivities using different animal models .(c) 2022 Elsevier B.V. All rights reserved.

Automated summary

Plant mediated nano-fabrication using Calotropis procera as a mediator was successfully used to synthesize copper oxide nanoparticles (CuONPs). The CuONPs exhibited anti-pathogenic activities against phytopathogens. Various characterization techniques confirmed the biofabrication of CuONPs and their potential applications in phytopathology.