Endophyte colonization enhanced cadmium phytoremediation by improving endosphere and rhizosphere microecology characteristics

This study investigated the phytoremediation efficiency of Cd-contaminated soils by hyperaccumulator P. acinosa and its endophyte B. cereus, and evaluated the variation of rhizosphere/endosphere microecology characteristics. The result showed that endophyte PE31, which could successfully colonize on P. acinosa root, increased plant Cd uptake by 42.90% and 28.85% in low and high Cd contaminated soils by promotion of plant biomass and Cd concentration in plant tissues. The improved phytoremediation may attribute to the endophyte inoculation, which significantly improved the bioavailable heavy metal (HM) percentage, nutrient cycling related enzyme activities and nutrient contents including available potassium, phosphorus and organic matter. Additionally, the relative abundance beneficial bacteria Bacillus (significantly increased by 81.23% and 34.03% in the endosphere, and by 4.86% and 8.54% in rhizosphere in low and high Cd contaminated soils) and Lysobacter, showed positive and close correlation with plant growth and HM accumulation. These results indicated that endophyte inoculation could reshape rhizosphere and endosphere micmecology characteristics, which enhanced the potential for phytoremediation of Cd contaminated soils.

Automated summary

This study found that inoculation of the endophyte B. cereus on hyperaccumulator P. acinosa can enhance the uptake of Cd by plants and improve their biomass, thus enhancing the phytoremediation potential of Cd-contaminated soils. The inoculation also led to changes in the rhizosphere and endosphere microecology characteristics.