期刊
JOURNAL OF CONTROLLED RELEASE
卷 225, 期 -, 页码 230-239出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jconrel.2016.01.050
关键词
Vaccine; Adjuvant; Immune response; Cationic-lipid PLGA hybrid nanoparticles; Encapsulation; Adsorption
资金
- Natural Science Foundation of China [50903093, 31200674, 51373199]
- Tianjin Natural Science Foundation [15JCYBJC18400, 15JCYBJC47600]
- PUMC Youth Fund
- Fundamental Research Funds for the Central Universities [33320140188]
In this study, we used cationic lipid-poly(lactide-co-glycolide) acid (PLGA) hybrid nanoparticles as antigen delivery carriers to investigate how antigen-loading methods affect antigen exposure to the immune system and evaluated the resulting antigen-specific immune responses. We formulated three classes of antigen adsorbed and/or encapsulated cationic lipid-PLGA hybrid nanoparticles; we designated antigen-adsorbed (out), antigenen-capsulated (in), and antigen-adsorbed/encapsulated (both) nanoparticles. Our results demonstrate significantly more efficient lysosomal escape and cross-presentation of antigen from dendritic cells (DCs) that were exposed to both and in nanoparticles. In vivo experiments further revealed that both nanoparticles significantly more effectively provided not only adequate initial antigen exposure but also long-term antigen persistence at the injection site. Data from flow cytometry and ELISA analyses demonstrated elevated in vivo immune responses from mice that were immunized with nanoparticles-delivered OVA when compared with free OVA. In addition, in and both nanoparticles elicited significantly higher antigen-specific immune response than out nanoparticles and free OVA. These results suggest that the location of antigen entrapment is an important factor in modulating the immune responses of antigens delivered by nanoparticles. Overall, we propose here a promising approach for the future design of vaccines using cationic lipid-PLGA nanoparticles. (C) 2016 Elsevier B.V. All rights reserved.
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