期刊
FOOD CHEMISTRY
卷 383, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2022.132293
关键词
Rapid detection; Xylazine; Monoclonal antibody; Immunoassay strip; Milk
资金
- National Key Research and Development Project [2018YFC1602903]
- National Science & Technology Pillar Program of 12th Five-Year Plan [2014BAD13B05]
A novel hapten was designed and synthesized for the artificial immunogen against xylazine, leading to the production of monoclonal antibody. This antibody was successfully applied in a lateral flow immunoassay strip for the rapid detection of xylazine in milk. The LFI strip showed a reliable and sensitive performance, providing a portable testing tool for on-site screening of xylazine.
In this study, a novel hapten was designed and successfully synthesized to prepared the artificial immunogen against xylazine (XYL), then the monoclonal antibody 1H2-2C6 was obtained and applied to a lateral flow immunoassay (LFI) strip for rapid detection of XYL in milk. The visual detection limit (cutoff value) of the LFI strip was 5 ng/mL, the linear regression formula was Y = -0.521lg(X) + 0.382 (R-2 = 0.995, n = 6), the linear detection range was 0.16-2.23 ng/mL and the detection limit (LOD) was 0.1 ng/mL. The recovery rates were in the range from 88.95% to 91.45%, the highest coefficient of variation was 8.89% (n = 3). The LFI strip was also validated by LC-MS/MS, as revealed, there were no significance differences between the two methods (the confidence interval was 95%). In brief, the LFI strip provides a reliable, sensitive and portable testing tool for screening of XYL on-site.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据