Article
Fisheries
Ian Bricknell, Michael Pietrak
Summary: This study investigates the environment of the digestive tract in fed and starved sea lice, with a focus on digestion and osmoregulation. The findings show that starved sea lice have a digestive tract similar in osmolarity to seawater. Even in lice with blood in their digestive system, the haemoglobin level is surprisingly low. Lice that have been feeding on salmon have a higher osmolarity in their digestive tract compared to starved animals, indicating a higher food content. The study confirms that sea lice are osmoconformers, with the haemolymph closely following the osmolarity of the environment.
Article
Multidisciplinary Sciences
Akiko Maruko, Koichi M. Iijima, Kanae Ando
Summary: This study investigates the molecular pathways involved in the generation of daily feeding patterns in Drosophila. The results show that quasimodo (qsm) and CLK play important roles in regulating feeding rhythms, and the generation of feeding/fasting episodes is independent of the molecular clock machinery.
Article
Fisheries
Diana Perez de Jesus, Martha Patricia Hernandez-Vergara, Carlos Ivan Perez-Rostro, Carlos Alfonso Frias-Quintana
Summary: The fasting/cyclic refeeding effect on freshwater prawn post larvae was evaluated over a period of 75 days in both biofloc and clear water cultures. The results showed that the prawns in biofloc culture had higher growth and enzymatic activity compared to those in clear water culture. The study confirmed the beneficial effects of biofloc technology on growth performance, digestive enzyme activity, and prawn health during short fasting periods.
AQUACULTURE INTERNATIONAL
(2023)
Article
Multidisciplinary Sciences
Alessandra Stangherlin, Joseph L. Watson, David C. S. Wong, Silvia Barbiero, Aiwei Zeng, Estere Seinkmane, Sew Peak Chew, Andrew D. Beale, Edward A. Hayter, Alina Guna, Alison J. Inglis, Marrit Putker, Eline Bartolami, Stefan Matile, Nicolas Lequeux, Thomas Pons, Jason Day, Gerben van Ooijen, Rebecca M. Voorhees, David A. Bechtold, Emmanuel Derivery, Rachel S. Edgar, Peter Newham, John S. O'Neill
Summary: This study reveals that circadian control of the proteome impacts ion homeostasis in cells, especially cardiomyocytes, through mechanisms involving osmotic compensation via electroneutral ion transport. Changes in ion content due to perturbation of soluble protein abundance drive daily rhythms in cardiomyocyte electrical activity, with substantial consequences for cell physiology.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Onelia Gagliano, Camilla Luni, Yan Li, Silvia Angiolillo, Wei Qin, Francesco Panariello, Davide Cacchiarelli, Joseph S. Takahashi, Nicola Elvassore
Summary: This study investigates the effect of oscillatory metabolic signals on the cell-autonomous clock in peripheral tissues, and highlights the role of synchronicity between life-style associated metabolic signals and peripheral clocks on circadian entrainment. The authors controlled cyclic metabolic signals in a microfluidic device to study the impact of glucose and insulin timing, period, and frequency on the transcriptome of cultured fibroblasts.
NATURE COMMUNICATIONS
(2021)
Article
Environmental Sciences
Jian Liang, Yuhang Liu, Feixia Zhu, Yongren Li, Shuang Liang, Yongjun Guo
Summary: This study examines the response of the digestive system of razor clams to ocean acidification. The results show that razor clams exhibit resistance to acidification in terms of phenotypic traits and physiological functions, but their digestive gland structure is directly affected.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Fisheries
Diana Barajas-Sandoval, Cristina Escobedo-Fregoso, Alberto Pena-Rodriguez
Summary: During the cultivation of Pacific white shrimp, environmental modifications and stress can affect the expected growth of organisms. Temporary stress can lead to a compensatory growth response, but extreme stress can cause a point of no return. This study aimed to evaluate the effect of temperature changes and temporary fasting on the transcriptomic compensatory growth response of the shrimp.
Article
Agriculture, Dairy & Animal Science
S. M. Keady, M. G. Keane, S. M. Waters, A. R. Wylie, E. G. O'Riordan, K. Keogh, D. A. Kenny
Summary: This study evaluated compensatory growth response in two contrasting breed types, Aberdeen Angus and Belgian Blue. The findings showed that both breeds were capable of compensatory growth after restricted feeding, but there were differences in the response between the breeds.
Article
Multidisciplinary Sciences
Jorge Giovanni Quintino-Rivera, Regina Elizondo-Gonzalez, Julian Gamboa-Delgado, Laura Teresa Guzman-Villanueva, Alberto Pena-Rodriguez
Summary: The present study aimed to evaluate the effects of compensatory growth on metabolic turnover rate, digestive enzyme activity, and bacterial biota in Pacific white shrimp. The results showed that there was a full compensatory growth in the shrimp subjected to feed restriction. The shrimp under feed restriction had lower metabolic turnover rates and longer nitrogen residence times in muscle tissue compared to the control group.
Article
Fisheries
Jeong-Hyeon Cho, Jin Woo Park, Bong-Joo Lee, Kang-Woong Kim, Sang-Woo Hur
Summary: This study investigates the effects of extrusion technology on the growth performance and digestive physiology of rockfish. The results show that small particle size and low extrusion pressure can enhance fish weight gain and plasma growth-related hormone activity. Furthermore, small particle size and low extrusion pressure also increase intestinal digestive activity and positively affect growth. It suggests that adjusting the physical pellet qualities through extrusion pressure and raw material particle size can improve the growth performance and digestive physiology of rockfish, which is important for feed manufacturing.
Article
Biochemistry & Molecular Biology
Yumeng Zhang, Hang Zhou, Yu Liu, Lulu Zhu, Jiongting Fan, Huajing Huang, Wen Jiang, Junming Deng, Beiping Tan
Summary: An 8-week feeding experiment was conducted to investigate the effect of dietary histamine on the growth performance, digestive physiology function and muscle quality of hybrid grouper. The results showed that high levels of histamine significantly decreased the activities of digestive enzymes, damaged the gastric and intestinal mucosa, disrupted the intestinal tight junction structure, and affected the muscle quality of the grouper.
Article
Endocrinology & Metabolism
Shiau Chin Chong, Norlela Sukor, Sarah Anne Robert, Kim Fong Ng, Nor Azmi Kamaruddin
Summary: This study aimed to resolve conflicting findings about GLP-1 levels in different glucose tolerance states among Malay, Chinese, and Indian ethnicities. The results showed that GLP-1 levels were higher in T2DM and pre-DM compared to NGT, suggesting a possible adaptive compensatory response. Among the three ethnic groups, Indians had the highest levels of GLP-1 and insulin resistance, supporting the notion of an adaptive compensatory secretion of GLP-1.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Fisheries
Malthe Hvas, Jonatan Nilsson, Tone Vagseth, Velimir Nola, Per Gunnar Fjelldal, Tom Johnny Hansen, Frode Oppedal, Lars Helge Stien, Ole Folkedal
Summary: The study shows that Atlantic salmon exhibit compensatory growth after fasting, eventually reaching similar weight and length as fed controls. Males were larger than females, and immature fish were larger than maturing fish. No aggressions were observed during the fasting period, and welfare status was not impacted in the short or long term.
Article
Multidisciplinary Sciences
Karolina Kwasek, Christian Gonzalez, Macdonald Wick, Giovanni S. Molinari, Michal Wojno
Summary: The study successfully developed an in vitro methodology for carp muscle hydrolysis using LMB endogenous digestive enzymes and found that the inclusion of carp muscle hydrolysate in the diet improved the growth performance of larval LMB, while also reducing skeletal deformities. The positive growth performance might be attributed to meeting the specific requirements of the larvae in terms of their digestive organ development, levels of digestive enzymes, and nutritional needs.
Review
Fisheries
Karin Hussy, Karin E. Limburg, Helene de Pontual, Oliver R. B. Thomas, Philip K. Cook, Yvette Heimbrand, Martina Blass, Anna M. Sturrock
Summary: Otolith chemistry is used to analyze various aspects of fish biology, with growing attention on physiological processes as a controlling factor. Biomineralization plays a critical role in governing otolith chemical patterns. Environmental tracers are dominated by elements that substitute for Ca or are randomly trapped within the crystal lattice.
REVIEWS IN FISHERIES SCIENCE & AQUACULTURE
(2021)
Article
Neurosciences
Francesca Vacca, Ana S. Gomes, Koji Murashita, Raffella Cinquetti, Cristina Roseti, Amilcare Barca, Ivar Ronnestad, Tiziano Verri, Elena Bossi
Summary: Two slc15a2-type genes were identified in the Atlantic salmon genome, encoding PepT2-type peptide transporters. While slc15a2a transcripts are widely distributed in fish tissues, abundant in the brain and gills, slc15a2b transcripts are mainly expressed in the kidney and distal gastrointestinal tract. Both PepT2a and PepT2b function as high-affinity transporters.
JOURNAL OF PHYSIOLOGY-LONDON
(2022)
Article
Biochemistry & Molecular Biology
Mariacristina Filice, Rosa Mazza, Sandra Imbrogno, Olga Mileti, Noemi Baldino, Amilcare Barca, Gianmarco Del Vecchio, Tiziano Verri, Alfonsina Gattuso, Maria Carmela Cerra
Summary: This study demonstrates for the first time the presence of an ACE2/Alamandine axis in goldfish and shows that it plays a role in modulating myocardial contractility through the NOS/NO system.
Article
Cell Biology
Adelfia Tala, Flora Guerra, Silvia Caterina Resta, Matteo Calcagnile, Amilcare Barca, Salvatore Maurizio Tredici, Maria Dolores De Donno, Mirco Vacca, Marina Liso, Marcello Chieppa, Maria De Angelis, Tiziano Verri, Maria Giuseppina Bozzetti, Cecilia Bucci, Pietro Alifano
Summary: This study demonstrates that the intestinal environment of mice with a missense mutation in the MUC2 mucin gene selects a specific intestinal microbial community characterized by pro-inflammatory, genotoxic, and metabolic features, suggesting its direct involvement in the pathogenesis of chronic intestinal inflammation.
Article
Biochemistry & Molecular Biology
Aurora Mazzei, Grazia Serino, Alessandro Romano, Emanuele Piccinno, Viviana Scalavino, Anna Maria Valentini, Raffaele Armentano, Roberta Schiavone, Gianluigi Giannelli, Tiziano Verri, Amilcare Barca
Summary: SLC15A4/PHT1 has been identified as a key path marker of immune/autoimmune/inflammatory pathways in immune cells, and this study found its overexpression in the colonic epithelium of ulcerative colitis (UC) patients. In vitro experiments successfully mirrored the inflammatory onset in spontaneously differentiated enterocyte-like monolayers of Caco-2 cells specifically exposed to DSS.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Chemistry, Multidisciplinary
Gianmarco Del Vecchio, Aurora Mazzei, Roberta Schiavone, Ana S. Gomes, Giovanni Frangelli, Tommaso Sala, Stefania Fantino, Marco G. A. Brocca, Amilcare Barca, Ivar Ronnestad, Tiziano Verri
Summary: Zebrafish is a widely used animal model in various research areas, including nutrigenomics and nutritional physiology. However, the standardization of its rearing conditions and feeding practices has not been achieved, hindering the generation of reliable and comparable results. This review discusses the limitations of current practices and introduces recent technological solutions, with a focus on automated feeding distribution systems. An overview of common schemes of zebrafish husbandry is also provided.
APPLIED SCIENCES-BASEL
(2022)
Article
Biology
Aurora Mazzei, Patrizia Pagliara, Gianmarco Del Vecchio, Lucia Giampetruzzi, Francesca Croce, Roberta Schiavone, Tiziano Verri, Amilcare Barca
Summary: The functionality of the enterocyte monolayer is directly impaired by inflammatory insults targeting the major cellular processes, including the cytoskeletal dynamics involving actin elements. Understanding the behavior of the actin cytoskeleton in enterocytes forming the epithelial monolayer is crucial for clarifying fundamental aspects of inflammatory mechanisms in the gastrointestinal tract.
Article
Chemistry, Analytical
Tiziano Di Giulio, Amilcare Barca, Tiziano Verri, Marco De Gennaro, Gabriele Giancane, Elisabetta Mazzotta, Cosimino Malitesta
Summary: We report the electrosynthesis of a metallo-porphyrin-based molecularly imprinted polymer (MIP) as a new receptor for carnosine recognition. The MIP was designed to utilize the metal-ion mediated recognition through the chelation of carnosine with Zn(II) ions. The MIP showed high response specificity, stability, and selectivity in the detection of carnosine and other histidine-bearing molecules.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Elisa Sciurti, Laura Blasi, Carmela Tania Prontera, Amilcare Barca, Lucia Giampetruzzi, Tiziano Verri, Pietro Aleardo Siciliano, Luca Francioso
Summary: Real-time monitoring of ions in cell culture systems and organ-on-a-chip platforms is a valuable investigation tool to understand ion regulation and distribution, as well as their involvement in biological mechanisms and specific pathologies. In this study, flexible sensors with ion selective membranes were developed and integrated into Transwell cell culture systems to investigate the transport of zinc and copper ions across intestinal cell monolayers. These sensors showed good sensitivity and low detection limits, making them suitable for pathophysiological studies. Additionally, electrochemical impedance spectroscopy and equivalent circuit fitting were used to monitor cellular barrier integrity. The proposed devices hold great potential for studying gut metabolism and permeability.
Article
Chemistry, Multidisciplinary
Vincenzo Nassisi, Aurora Mazzei, Gianmarco Del Vecchio, Antonio Calisi, Luciano Velardi, Pietro Alifano, Tiziano Verri
Summary: In this experiment, groups of zebrafish embryos were exposed to radiations of different frequencies and intensities using new prototype devices. The radiations included static, extremely low-frequency, low-frequency, very-high-frequency, and ultra-high-frequency fields. The exposure led to alterations in body pigmentation of the zebrafish embryos and larvae. These findings contribute to understanding the effects of magnetic radiation on living organisms.
APPLIED SCIENCES-BASEL
(2023)
Article
Pharmacology & Pharmacy
Nunzia Gallo, Stefano Quarta, Marika Massaro, Maria Annunziata Carluccio, Amilcare Barca, Donato Cannoletta, Luisa Siculella, Luca Salvatore, Alessandro Sannino
Summary: Vascularization is a complex and precise process, but it can be slow and inadequate for tissue repair. Biomaterial-based strategies have been developed to enhance vasculogenesis. In this study, PLGA microparticles loaded with L-lysine were developed for controlled release. The optimized formulation showed biocompatibility and supported cell proliferation and angiogenesis. L-lysine encapsulated in PLGA microparticles has potential as a cost-effective system for angiogenesis enhancement and rapid healing.
Article
Fisheries
Amilcare Barca, Francesca Abramo, Sareh Nazerian, Francesca Coppola, Chiara Sangiacomo, Carlo Bibbiani, Rosario Licitra, Francesca Susini, Tiziano Verri, Baldassare Fronte
Summary: Insect meal is considered a promising alternative to fishmeal for improving aquafeed sustainability. This study investigated the effects of increasing the inclusion of Hermetia illucens meal on fish mortality, growth performance, intestine morphology, and gene expression of intestinal carriers. The results showed no adverse effects on fish mortality and body weight, with a positive effect on feed conversion ratio. The use of Hermetia illucens larvae meal as a replacement for fishmeal in aquafeeds can enhance sustainability and promote favorable fish growth performance and gut health.
Review
Environmental Sciences
Maria Giulia Lionetto, Vincenzo Zonno, Roberta Schiavone, Maria Elena Giordano, Amilcare Barca, Genuario Belmonte, Tiziano Verri
Summary: Transitional waters are precious habitats for rare and endangered species, but they are vulnerable to human-induced threats. This review examines the suitability of Aphanius fasciatus as a sentinel species for assessing the quality of transitional water environments. The study suggests that the molecular, cellular, and physiological responses of A. fasciatus can serve as diagnostic tools for detecting the subtle effects of environmental pollution. Omics technologies show great potential in understanding organisms' responses to environmental changes, highlighting the need to improve omics tools for A. fasciatus in the future.