Article
Engineering, Biomedical
Xing Liu, Jianxin Sui, Cui Li, Qian Wang, Xudong Peng, Fanyue Meng, Qiang Xu, Nan Jiang, Guiqiu Zhao, Jing Lin
Summary: In this study, a beta-glucan-specific nanobody was developed and loaded with natamycin to effectively treat fungal keratitis. The nanobody exhibited antifungal effects through inhibiting fungal growth, disrupting cell wall and biofilm formation, and reducing adhesion ability to corneal epithelial cells. The conjugation with the nanobody significantly improved the antifungal capacity of natamycin and reduced its toxicity. The nanobody also attenuated fungal virulence and relieved inflammatory responses in fungal keratitis.
ACTA BIOMATERIALIA
(2023)
Article
Pharmacology & Pharmacy
Thirumurthy Velpandian, Jayabalan Nirmal, Hanuman P. Sharma, Sanjay Sharma, Namrata Sharma, Nabanita Halder
Summary: This study aimed to develop a novel water soluble formulation of natamycin for the treatment of fungal keratitis, which showed promising efficacy in various tests and was well tolerated in animal studies. The new formulation has the potential to improve patient compliance and provide an effective alternative to the existing topical suspension.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Article
Mycology
Daisuke Todokoro, Tomoko Miyakubo, Koichi Makimura, Takashi Tamura, Aya Komori, Hideo Akiyama
Summary: Fungal keratitis caused by Talaromyces species, which is a rare fungus, has been reported for the first time in this case. The patient's condition gradually improved after treatment.
Article
Mycology
Sho Arimoto, Katsuhiro Inagaki, Daisuke Todokoro, Takashi Suzuki, Koichi Makimura, Tomoko Ishino
Summary: This study evaluated the therapeutic effects of luliconazole, an imidazole antifungal drug, in a rabbit model of fungal keratitis caused by filamentous fungi. The results showed that luliconazole demonstrated therapeutic efficacy comparable to natamycin, suggesting that it could be a novel therapeutic agent for human fungal keratitis.
Article
Pharmacology & Pharmacy
Victoria Diaz-Tome, Carlos Bendicho-Lavilla, Xurxo Garcia-Otero, Ruben Varela-Fernandez, Manuel Martin-Pastor, Jose Llovo-Taboada, Pilar Alonso-Alonso, Pablo Aguiar, Miguel Gonzalez-Barcia, Anxo Fernandez-Ferreiro, Francisco J. Otero-Espinar
Summary: The study developed natamycin/voriconazole formulations to improve the treatment of fungal keratitis by enhancing the solubility, permanence, and safety of natamycin. These formulations demonstrated improved corneal permeability and antifungal activity, suggesting their potential significance in treating fungal keratitis.
Article
Ophthalmology
Songying Luan, Xudong Peng, Jing Lin, Yingxue Zhang, Lu Zhan, Jiao Yin, Junjie Luan, Xiaoyue Ji, Guiqiu Zhao
Summary: Gallic acid alleviates Aspergillus fumigatus keratitis severity by inhibiting fungal load, reducing neutrophil infiltration, downregulating pro-inflammatory cytokines expression, and enhancing the Nrf2/HO-1 pathway.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Article
Pharmacology & Pharmacy
Khaled M. Hosny, Waleed Y. Rizg, Hala M. Alkhalidi, Walaa A. Abualsunun, Rana B. Bakhaidar, Alshaimaa M. Almehmady, Adel F. Alghaith, Sultan Alshehri, Amani M. El Sisi
Summary: The study successfully optimized an NT-loaded cubosome formulation and converted it into an in situ gel system, which effectively enhanced the ocular permeation and antifungal activity of natamycin, reduced ocular irritation, and prolonged retention time within the eye.
Article
Food Science & Technology
Pingping Tang, Tingting Zheng, Ruimin Ran, Yongming Xiong, Guoying Li
Summary: Collagen films were functionalized with gallic acid (GA) at different concentrations using laccase. FTIR analysis showed the formation of crosslinking bonds between collagen and GA. Increasing GA concentration resulted in stronger crosslinking and improved stability of GA in the film. At a GA concentration of 8 mmol, the collagen film had increased tensile strength and denaturation temperature. Films with GA concentration ≥ 4 mmol exhibited scavenging activity against DPPH radicals and antibacterial activity against S. aureus and E. coli. The film with 8 mmol GA showed potential for food packaging by reducing spoilage during beef storage.
FOOD PACKAGING AND SHELF LIFE
(2023)
Article
Chemistry, Applied
Ying Li, Lezhen Dong, Yahui Liu, Qin Chen, Zufang Wu, Lingyi Liu, Mohamed A. Farag, Lianliang Liu
Summary: The effects of covalent binding of protocatechuic acid (PA) and gallic acid (GA) to lactoferrin (LF) on the structure, functional, and antioxidant properties of the protein conjugate were investigated. The LF-PA/GA conjugates showed altered hydrodynamic diameter and zeta-potential, but their antioxidant activities remained unchanged.
Article
Medicine, General & Internal
Jingjing Cai, Chengwen Yang, Qiuhong Wei, Huifang Lian, Lin An, Rong Zhang
Summary: The study aims to observe the therapeutic effect of natamycin combined with voriconazole in the treatment of fungal keratitis. The results showed that the total efficacy of the treatment group was significantly higher than that of the control group, with shorter disappearance time and lower keratitis severity score, indicating good efficacy and safety.
PAKISTAN JOURNAL OF MEDICAL SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Harsha Rohira, Sujithra Shankar, Shikha Yadav, Sushmita G. Shah, Archana Chugh
Summary: Tat(2)natamycin, a conjugate with cell penetrating peptides (CPPs), demonstrates enhanced ocular penetration and antifungal efficacy compared to natamycin alone in treating fungal keratitis.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Chemistry, Applied
Hui Wang, Shengping You, Wenhang Wang, Yan Zeng, Rongxin Su, Wei Qi, Kang Wang, Zhimin He
Summary: The study found that laccase can promote the cross-linking of gallic acid and soy protein isolate under neutral pH conditions. Gallic acid significantly enhanced the antioxidant activity of soy protein isolate, increasing DPPH free radical scavenging rate, ABTS free radical scavenging ability, and reducing power.
Article
Ophthalmology
Gunjan Saluja, Namrata Sharma, Rinky Agarwal, H. P. Sharma, Praful Maharana, Gita Satpathy, Jeewan Singh Titiyal, T. Velpandian
Summary: This study evaluated the outcomes of water-soluble intrastromal natamycin as an adjunct therapy for recalcitrant fungal keratitis. With IS-NTM injection, no serious adverse reactions were observed, and the overall cure rate was 95%, with an average resolution time of 6 weeks.
INDIAN JOURNAL OF OPHTHALMOLOGY
(2021)
Article
Ophthalmology
Jeremy J. Hoffman, Reena Yadav, Sandip D. Sanyam, Pankaj Chaudhary, Abhishek Roshan, Sanjay K. Singh, Sailesh K. Mishra, Simon Arunga, Victor H. Hu, David Macleod, Astrid Leck, Matthew J. Burton
Summary: This study aimed to investigate whether the use of low-cost 0.2% chlorhexidine gel is noninferior to 5% topical natamycin for the treatment of filamentous fungal keratitis. The results showed that natamycin treatment is associated with significantly better visual acuity and fewer adverse events compared to chlorhexidine treatment.
Review
Medicine, General & Internal
Marek Szalinski, Aleksandra Zgryzniak, Izabela Rubisz, Malgorzata Gajdzis, Radoslaw Kaczmarek, Joanna Przezdziecka-Dolyk
Summary: The management of Fusarium keratitis is challenging due to delays in the onset of symptoms from an injury and difficulties in differentiating fungal from bacterial infections. Therapeutic options are limited, including antifungals, antiseptics, and surgical interventions.
JOURNAL OF CLINICAL MEDICINE
(2021)