Isolation of Some Phenolic Compounds from Plantago subulata L. and Determination of Their Antidiabetic, Anticholinesterase, Antiepileptic and Antioxidant Activity

In the current study, some phenolic compounds, including acteoside, isoacteoside, echinacoside, and arenarioside purified and characterized from Plantago subulata. These compounds were tested for its antioxidant potential, including Fe3+ and Cu2+ reductive ability and Fe2+ chelating effects. The inhibitory effects of isolated phenolic compounds were tested towards human carbonic anhydrase I and II isoenzymes (hCA I and hCA II), butyrylcholinesterase (BChE) acetylcholinesterase (AChE), aldose reductase (AR) and alpha-glycosidase (alpha-gly). K-i values were found these compounds in range of 0.24 +/- 0.05-1.38 +/- 0.34 mu M against hCA I, 0.194 +/- 0.018-1.03 +/- 0.06 mu M against hCA II, 0.043 +/- 0.01-0.154 +/- 0.02 mu M against AChE, 3.92 +/- 1.08-11.93 +/- 4.45 mu M against BChE, 0.082 +/- 0.0008-1.68 +/- 0.42 mu M against AR, and 6.93 +/- 2.74-17.17 +/- 6.70 mu M against alpha-glycosidase. As a result, isolated compounds displayed inhibition effects against studied all metabolic enzymes. They are promising candidates for treating disorders like Alzheimer's disease, diabetes mellitus, glaucoma, leukemia, and epilepsy.

Automated summary

In this study, phenolic compounds purified from Plantago subulata were tested for their antioxidant potential and inhibitory effects on metabolic enzymes. The compounds showed inhibition effects against all tested enzymes and could be potential candidates for treating various disorders.