期刊
CARBOHYDRATE POLYMERS
卷 290, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2022.119482
关键词
Carboxymethyl chitosan; Carboxymethyl cellulose; Quartz crystal microbalance; Piezoelectric biosensor; Immunoassay
资金
- Natural Science Foundation for Distinguished Young Scholars of Hubei Province of China [2020CFA105]
- Basic Research Project of Science and Technology Plan of Shenzhen [JCYJ20180305164647940]
This study demonstrates a piezoelectric biosensor with CMCS as the immobilization matrix for rapid, label-free, and cost-effective quantification of IgY.
Immunoglobulin Y (IgY) proves advantageous to IgG in prophylaxis and diagnosis. Quantification of IgY is therefore becoming a topic of interest. Here, we demonstrate a piezoelectric biosensor with carboxymethyl chitosan (CMCS) as the immobilization matrix. Gelation and hydrophilic nature of CMCS are favored to form a crosslinked matrix for antibody immobilization, and a comparison was made between carboxymethyl cellulose (CMC) and CMCS to investigate the benefits of such substitution. Calibration from 500 ng/mL to 200 mu g/mL was established in buffer with the detection limit (LOD) down to 270 ng/mL, confirming its feasibility. As-prepared biosensor effectively prevents non-specific binding of bovine serum albumin (BSA) and lysozyme. Each real-time assay took 15 min including sensor regeneration, which can be further reduced to 4 min for signal readout only, ready for both repeated measurements after regeneration and disposable devices. Thus, as-prepared biosensor offers a rapid, label-free and cost-effective approach for IgY quantification.
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