Article
Chemistry, Analytical
Ciro Rodriguez Diaz, Nuria Lafuente-Gomez, Catarina Coutinho, Demian Pardo, Hernan Alarcon-Iniesta, Maria Lopez-Valls, Rocio Coloma, Paula Milan-Rois, Mirian Domenech, Melanie Abreu, Rafael Canton, Juan Carlos Galan, Rebeca Bocanegra, Luis A. Campos, Rodolfo Miranda, Milagros Castellanos, Alvaro Somoza
Summary: This article presents a colorimetric sensor based on gold nanoparticles for the detection of SARS-CoV-2 sequences. The sensor is fast, reliable, and easily scalable, with the ability to detect target sequences within 15 minutes. The efficiency and selectivity of detection are evaluated using synthetic samples, infected cells, and patient samples. The system's sensitivity is enhanced through an affordable amplification approach, enabling the detection of high and medium viral loads. The proposed approach is valuable for facilities with limited resources due to its ease of use, stability, and minimal equipment requirements.
Article
Chemistry, Analytical
Wei S. Zhang, Jianbin Pan, Feng Li, Min Zhu, Mengting Xu, Hongyan Zhu, Yanyan Yu, Gaoxing Su
Summary: The proposed method combines RT-RPA with CRISPR-Cas12a colorimetric assay for SARS-CoV-2 detection, utilizing DNA-modified gold nanoparticles to achieve high sensitivity and specificity. This novel approach can effectively avoid false positive results from other beta coronavirus members, improving the practical testing availability of SARS-CoV-2.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Xiaojing Pei, Hu Hong, Sitong Liu, Na Li
Summary: This study developed a simple and sensitive method for Mycobacterium tuberculosis-specific DNA detection using dark-field microscopic imaging and rolling-circle amplification. The proposed method showed high sensitivity and feasibility for application in biological samples, providing a new tool for pathogen detection.
Article
Food Science & Technology
Marzieh Sepahvand, Forough Ghasemi, Hossein Mir Seyed Hosseini
Summary: This paper discusses the serious threat of irregular and unknowing use of chemical compounds to the environment, human health, and living organisms, emphasizing the importance of monitoring nitrite and nitrate. It comprehensively discusses the application and advantages of nanoplasmonic colorimetric sensors in the measurement of nitrite and nitrate.
FOOD AND CHEMICAL TOXICOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Kai-Jen Chuang, Meng-Ru Dong, Purnima Laishram, Gui-Bing Hong
Summary: A colorimetric method based on sodium nitrite-functionalized gold nanoparticles was developed for the detection of acenaphthene and naphthalene. The method showed a linear relationship between the analyte concentration range of 0.1-10 ppm and had a limit of detection of 0.046 ppm for acenaphthene and 0.0015 ppm for naphthalene. The recovery rate for actual samples was found to be within the range of 98.4-103.0%. In selective and anti-interference tests, the presence of cations and anions had minimal impact on the detection of the analyte. This study provides an effective colorimetric method for detecting the presence of acenaphthene and naphthalene in real water samples with a high recovery rate.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Spectroscopy
Chih-Ming Ma, Li-Chieh Lin, Kai-Jen Chuang, Gui-Bing Hong
Summary: A novel colorimetric detection method based on gold nanoparticles was developed for the determination of phenanthrene and pyrene. The method exhibited good detection ability and selectivity after parameter optimization.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Food Science & Technology
Shengnan Wei, Zhenyue Su, Xiangong Bu, Xuening Shi, Bo Pang, Liang Zhang, Juan Li, Chao Zhao
Summary: A colorimetric assay aptasensor for the detection of Salmonella typhimurium was developed using gold nanoparticles embedded spherical covalent organic framework with peroxidase-like activity and the affinity and specificity of S. typhimurium-aptamer. The aptasensor showed a low detection limit and good recovery rates, and can be combined with a smartphone-based image analysis system for point-of-care testing.
NPJ SCIENCE OF FOOD
(2022)
Article
Chemistry, Analytical
Hongfei He, Yan Zhou, Bin Chen, Yi Zhang, Xiaowu Zhong, Lei Xu, Bin Guo, Chong Yin, Xi Zhou, Qingrong Li, Zhen Huang, Guangcheng Luo, Xiaolan Guo
Summary: A colorimetric isothermal sensing platform (SFMC) with specific signal filtration and magnification has been developed for ultrasensitive detection of DNA and RNA. This platform can detect target DNA as low as 5 copies per reaction and target RNA as low as 10 copies per reaction by naked eyes. It has also been successfully used for the detection of African swine fever virus (ASFV) and SARS-CoV-2.
Article
Chemistry, Multidisciplinary
Miseon Jeong, Dahyun Bae, Jin-sil Choi
Summary: Due to the neural toxicity of mercury, a new colorimetric mercury detection probe, Fe3O4@SiO2@Au (magnetic-Au; Mag-Au) hybrid nanoparticles, has been developed for on-site detection of Hg2+. The presence of Hg2+ forms an AuHg alloy (amalgam) on the surface of Mag-Au, with excellent peroxidase-like activity. The oxidation of a substrate leads to a color change, allowing the detection of Hg2+ at nanomolar concentrations. Magnetic separation can be used to purify and concentrate the Mag-Au@Hg from samples, avoiding interference from unwanted residues or colored samples. The feasibility of Mag-Au for Hg2+ detection has been validated with artificial urine solution and can be applied to various real samples.
NANOSCALE ADVANCES
(2023)
Article
Nanoscience & Nanotechnology
Yuxiang Xue, Xinyao Ma, Xue Feng, Sam Roberts, Guangyu Zhu, Yi Huang, Xianfeng Fan, Jun Fan, Xianfeng Chen
Summary: Gold nanostructures, especially gold nanobipyramids, have gained significant attention due to their unique physical and optical properties. However, synthesizing and purifying homogeneous gold nanobipyramids is a challenging task.
ACS APPLIED NANO MATERIALS
(2023)
Article
Multidisciplinary Sciences
George S. Luka, Ephraim Nowak, Quin Robert Toyata, Nishat Tasnim, Homayoun Najjaran, Mina Hoorfar
Summary: The study developed a portable, colorimetric biosensor for sensitive, selective, and label-free detection of Cryptosporidium RNA. This sensor eliminates the need for expensive laboratory equipment, instead performing colorimetric assays on a micro-fabricated chip in a 3D-printed holder assembly, with quantitative analysis done using a smartphone camera.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Applied
Madhusudhan Alle, Soo Chan Park, Rajkumar Bandi, Seung-Hwan Lee, Jin- Chul Kim
Summary: A novel microwave-assisted green in-situ synthesis of positively charged gold nanoparticles supported by cationic cellulose nanofibrils within 30 s is reported. The peroxidase activity of these positive AuNPs was found to be superior over its negative charged counterpart. The film made from AuNPs@C.CNF can be reused for the detection of glucose up to five cycles without a decrease in activity, with comparable results to the standard GOD-POD method for quantifying glucose in human serum samples.
CARBOHYDRATE POLYMERS
(2021)
Article
Engineering, Biomedical
Xiaoyi Ma, Wuping Zhou, Haiwen Li, Bo Zhang, Peng Miao
Summary: In this study, the protection of hetero-nanostructure of MnO2 and AuNPs from salt-induced aggregation was systematically investigated using DNA as a medium. By analyzing the optical responses of these nanostructures, highly sensitive analysis of miRNA can be achieved. The excellent analytical performances are attributed to the unique features of MnO2@Au nanostructures and signal amplification designs, making them promising for the construction of novel biosensors in clinical applications.
MATERIALS TODAY BIO
(2023)
Article
Chemistry, Multidisciplinary
Noemi Bellassai, Roberta D'Agata, Almudena Marti, Andrea Rozzi, Stefano Volpi, Matteo Allegretti, Roberto Corradini, Patrizio Giacomini, Jurriaan Huskens, Giuseppe Spoto
Summary: A new nanoparticle-enhanced assay simplifies the detection of tumor DNA in patient plasma, while a dual-functional low-fouling PLL-based surface layer allows for the detection of mutated DNA at attomolar levels without PCR amplification.
Article
Biotechnology & Applied Microbiology
Fernanda Raquel Wust Schmitz, Karina Cesca, Alexsandra Valerio, Debora de Oliveira, Dachamir Hotza
Summary: Novel rapid methodologies involving colorimetric biosensors and gold nanoparticles have been developed for the detection of bacteria, specifically Pseudomonas aeruginosa. The biosensor relies on the color change of gold nanoparticles induced by NaCl after incubation with bacteria and aptamer binding. The optimized conditions for detection were found to be 5 μM aptamer concentration and 200 mM of NaCl. The detection limit for P. aeruginosa was 10(5) CFU mL(-1) by the naked eye and 10(4) CFU mL(-1) by UV-Vis spectrometry.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Fengyun Li, Danya Lyu, Shuo Liu, Weiwei Guo
ADVANCED MATERIALS
(2020)
Article
Chemistry, Multidisciplinary
Chi Yao, Han Tang, Weijian Wu, Jianpu Tang, Weiwei Guo, Dan Luo, Dayong Yang
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2020)
Article
Chemistry, Multidisciplinary
Shuo Liu, Mingjie Yang, Weiwei Guo
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
(2020)
Article
Chemistry, Multidisciplinary
Xiaoxue Du, Yanhui Bi, Pingping He, Chunyan Wang, Weiwei Guo
ADVANCED FUNCTIONAL MATERIALS
(2020)
Article
Chemistry, Multidisciplinary
Yanhui Bi, Xiaoxue Du, Pingping He, Chunyan Wang, Chang Liu, Weiwei Guo
Article
Chemistry, Multidisciplinary
Cuiyan Han, Weiwei Guo
Article
Nanoscience & Nanotechnology
Jing Yang, Tingting Wang, Ran Guo, Decui Yao, Weiwei Guo, Shuo Liu, Zhiqiang Li, Yige Wang, Huanrong Li
ACS APPLIED MATERIALS & INTERFACES
(2020)
Article
Chemistry, Analytical
Chunyan Wang, Cuiyan Han, Xiaoxue Du, Weiwei Guo
Summary: A versatile CRISPR-Cas12a-based biosensing platform was developed in this study by introducing an entropy-driven dynamic DNA network, allowing detection of various types of targets with low detection limits for nucleic acid and protein analytes. The platform also demonstrated good applicability in human serum samples.
ANALYTICAL CHEMISTRY
(2021)
Article
Green & Sustainable Science & Technology
Xinran Xu, Ping-Ping He, Xiaoxue Du, Weiwei Guo
Summary: This study presents a method to regulate the catalytic activity of DNAzymes through temperature and near-infrared light irradiation. DNAzymes are immobilized into polymeric matrix pNIPAM microgels prepared with a microfluidic device, allowing for reversible regulation of the DNAzyme activity. The catalytic activity of DNAzyme can also be remotely controlled using photothermal MXene nanosheets.
ADVANCED SUSTAINABLE SYSTEMS
(2022)
Article
Materials Science, Multidisciplinary
Shanjin Hu, Xiaoxue Du, Yanhui Bi, Ping-Ping He, Yali Mu, Chang Liu, Qi Gao, Mengyuan Yin, Weiwei Guo
Summary: This study reports on the construction of responsive DNA hydrogel based on the self-assembly of one short single-stranded DNA, which can be used to prepare conductive polymers with excellent catalytic activity. By programming the DNA chain sequence, responsive DNA hydrogels can also be constructed, holding potential in soft electronic and biosensing applications.
ACS APPLIED POLYMER MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Xiaoxue Du, Ping-Ping He, Chunyan Wang, Xiaowen Wang, Yali Mu, Weiwei Guo
Summary: Inspired by the respiration systems of organisms, researchers have developed an active strategy for highly efficient biomolecular substance transport through the thermo-stimulated inhalation-exhalation cycles of hydrogel matrices. This strategy improves the functional properties of hydrogel systems and accelerates the intake of external substances and expelling of endogenous substances.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Ping-Ping He, Xiaoxue Du, Yuan Cheng, Qi Gao, Chang Liu, Xiaowen Wang, Yonghua Wei, Qilin Yu, Weiwei Guo
Summary: This study establishes a promising platform for highly efficient photothermal-chemo synergistic cancer therapy by integrating DNA hydrogels with MXene-based photothermal agent and doxorubicin as a loaded chemotherapeutic agent. The temperature rise caused by photothermal MXene nanosheets triggers the reversible gel-to-solution transition, releasing therapeutic agents for efficient localized cancer therapy.
Article
Biophysics
Chunyan Wang, Yaxing Zhang, Chang Liu, Siyu Gou, Shanjin Hu, Weiwei Guo
Summary: To overcome the limitations of conventional miRNA biosensing devices, researchers have developed a portable and smart colorimetric analysis platform using DNA-gold nanoparticles and an enzyme-free dynamic DNA network. By programming the DNA sequences, the dynamic DNA network can respond to specific miRNA and trigger the release of gold nanoparticles from the hydrogel film as a colorimetric signal output. Digital analysis based on primary three-color channel was introduced to achieve a detection limit at pM level. Furthermore, by introducing a H2O2-mediated gold nanoparticles enlargement procedure, the detection limit for miRNA target could be further enhanced to fM level. The portable and storable platform exhibits good storage stability for at least 45 days, showing great potential in practical diagnosis applications.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Multidisciplinary
Yaxing Zhang, Chunyan Wang, Mengyuan Yin, Hanxue Liang, Qi Gao, Shanjin Hu, Weiwei Guo
Summary: Smart DNA microgels have excellent biocompatibility and sequence-directed functions, making them attractive for research. By combining DNA microgels with gallium-based liquid metals (LMs), their applications in biosensing and biomedicine can be further expanded. The combination of LM particles and polymeric matrix allows the construction of soft-soft materials, and the localized polymerization reaction enables the precise control of microgel compositions and the construction of microgels with tunable catalytic activities. Additionally, the photothermal effect of LM NPs allows for the remote control of microgel activity through NIR light irradiation.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)
