Article
Microbiology
Elena B. Popowitch, Sam Kaplan, Zenglin Wu, Yi-Wei Tang, Melissa B. Miller
Summary: This study compares the performance of three widely used panels for respiratory syndromic testing. The results show that BioFire and Luminex assays have similar overall accuracy, while significant differences are found between BioFire and GenMark, as well as between GenMark and Luminex.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
Kirstine K. Sogaard, Vladimira Hinic, Daniel Goldenberger, Alexander Gensch, Michael Schweitzer, Veronika Battig, Martin Siegemund, Stefano Bassetti, Roland Bingisser, Michael Tamm, Manuel Battegay, Maja Weisser, Daiana Stolz, Nina Khanna, Adrian Egli
Summary: This study evaluated the diagnostic performance of the Biofire(©) FilmArray pneumonia (PN)-panel against standard culture in broncho-alveolar lavage (BAL) samples. The results showed that the PN-panel had high sensitivity and specificity, but low positive predictive value. The detection rate of bacterial pathogens was higher in the PN-panel compared to culture. Further evaluation is needed to assess the impact of the PN-panel on clinical management.
Article
Infectious Diseases
Yu Kyung Kim, Jong Ho Lee, Sae Yoon Kim, Ji Young Ahn, Kwang Hae Choi, Young Hwan Lee, Kyung Mi Jang, Yong Sauk Hau, Jae Min Lee
Summary: Multiplex polymerase chain reaction (mPCR) is increasingly used for diagnosing respiratory pathogens in pediatric inpatient facilities, with the adapted FilmArray Respiratory Panel (FA-RP) offering faster and more accurate detection of multiple viruses, leading to reduced antibiotic use and hospital stays in pediatric patients.
Article
Pediatrics
Niko Paalanne, Minna Honkila, Suvi Mattila, Tytti Pokka, Marjo Renko, Terhi Tapiainen
Summary: This study investigated the duration of clinical symptoms associated with different respiratory viruses and the co-detection of respiratory viral and bacterial pathogens. The results showed that persistent symptoms were common, especially in children, and viral and bacterial co-detection may prolong the illness.
Article
Microbiology
Linqing Zhao, Xiaoyan Dong, Ling Cao, Yongmei Jiang, Hong Zhang, Ran Mo, Hanmin Liu
Summary: This study evaluated the performance of Acaruiter Respiratory Panel (fluorescent PCR) in detecting respiratory tract infection pathogens in children. The results showed that it has good consistency, high accuracy, and high positive and negative agreement percentages.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Gregory J. Berry, Wei Zhen, Elizabeth Smith, Ryhana Manji, Suzane Silbert, Amorce Lima, Amanda Harington, Kathleen McKinley, Bart Kensinger, Crissy Neff, Daisy Lu
Summary: As the incidence of SARS-CoV-2 overlaps with the traditional respiratory season, simultaneous testing for SARS-CoV-2 and other respiratory infections is crucial. The BioFire RP2.1 assay showed excellent performance in detecting SARS-CoV-2, with highly concordant results compared to the composite result.
JOURNAL OF CLINICAL MICROBIOLOGY
(2022)
Article
Infectious Diseases
Fen Pan, Bingjie Wang, Hong Zhang, Yingying Shi, Qi Xu
Summary: This study enrolled 90 children, among whom 58 patients (64.4%) tested positive for 13 pathogens using FARP, with 18 patients showing multiple-virus infections. Human rhinovirus/enterovirus were most common in infants and toddlers, while adenovirus were common in the 3-5-year-old group. Only three pathogens were found in the 6-14-year-old group.
BMC INFECTIOUS DISEASES
(2021)
Article
Medicine, General & Internal
Li-Min Xie, Xin Yin, Tian-Ao Xie, Jian-Wen Su, Qin Huang, Jing-Hao Zhang, Yin-Fei Huang, Xu-Guang Guo
Summary: This study evaluated the diagnostic efficacy of the Luminex xTAG Respiratory Virus Panel (RVP) FAST v2 test on respiratory viral infections. Results showed that the test had high specificity and sensitivity, making it a reliable and rapid diagnostic method for multiple respiratory viral infections.
YONSEI MEDICAL JOURNAL
(2022)
Article
Medicine, General & Internal
Harshad Lade, Jung-Min Kim, Yousun Chung, Minje Han, Eun-Kyung Mo, Jae-Seok Kim
Summary: This study evaluated the performance of two multiplex nucleic acid amplification assays for simultaneous detection of multiple respiratory pathogens. The results showed consistent performance between the Allplex Respiratory Panel and the BioFire FilmArray, with some variations in detection rates for specific pathogens.
Article
Medicine, General & Internal
Jinbao Huang, Heng Weng, Ling Ye, Meiqin Jiang, Yangyu Li, Hongyan Li
Summary: This study evaluated the clinical value of metagenomic next-generation sequencing (mNGS) for the screening of lower respiratory tract infections (LRTIs) and tumors. The results showed that mNGS had higher sensitivity and accuracy in pathogen diagnosis compared to traditional culture and comprehensive microbial tests (CMT). In addition, CNV detection was found to be an important auxiliary diagnostic tool for cancer.
INTERNATIONAL JOURNAL OF GENERAL MEDICINE
(2023)
Article
Microbiology
Xin Zhang, Colleen Knoth, Anh Pham, Amorce Lima, Rosario Dominguez, Irvin Ibarra-Flores, Juan C. Lopez, Dominic Uy, Suzane Silbert, Anami Patel, Michael Aye, Yi-Wei Tang, Jennifer Dien Bard
Summary: Early detection of respiratory tract pathogens is crucial for clinical management. The BioCode Respiratory Pathogen Panel utilizes RT-PCR and barcoded magnetic beads to detect and identify 17 pathogens. A multicenter clinical trial showed comparable performance between BioCode RPP and FilmArray Respiratory Panel. This high-throughput test offers automated testing capability and a sample-to-result time under 5 hours.
MICROBIOLOGY SPECTRUM
(2023)
Article
Immunology
Eric J. Chow, Amanda M. Casto, Pavitra Roychoudhury, Peter D. Han, Hong Xie, Brian Pfau, Tien Nguyen, Jaydee Sereewit, Julia H. Rogers, Sarah N. Cox, Caitlin R. Wolf, Melissa A. Rolfes, Emily Mosites, Timothy M. Uyeki, Alexander L. Greninger, James P. Hughes, M. Mia Shim, Nancy Sugg, Jeffrey S. Duchin, Lea M. Starita, Janet A. Englund, Helen Y. Chu
Summary: Rhinovirus (RV) infections were found to persist in homeless shelters, with the highest prevalence observed in shelters housing families. The study also revealed a diverse range of circulating RV serotypes, each detected over short periods of time. Community-based surveillance in congregate settings is crucial for understanding respiratory viral infections during and after the COVID-19 pandemic.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Medicine, General & Internal
David Carmon, Hanan Rohana, Maya Azrad, Avi Peretz
Summary: This study utilized the FilmArray((R)) Gastrointestinal Panel to investigate gastrointestinal infection and pathogen distribution in stool samples of hospitalized patients in Israel. The most common pathogen detected was Campylobacter. Positive samples were associated with younger age, increased use of antibiotics, and shorter hospital stay and time to discharge compared to negative samples.
Article
Biochemistry & Molecular Biology
Ying-Ju Chen, Tze-Kiong Er
Summary: This study examined the distribution of respiratory viral pathogens during the COVID-19 pandemic in an emergency department. The most common pathogen detected was rhinovirus/enterovirus, followed by adenovirus and SARS-CoV-2. The results showed perfect agreement between the cobas Liat system and the FilmArray TM RP for SARS-CoV-2 detection. The FilmArray TM RP assay is a reliable method for detecting SARS-CoV-2 and other respiratory infections.
Article
Infectious Diseases
Nabil Gastli, Julien Loubinoux, Matthieu Daragon, Jean-Philippe Lavigne, Pierre Saint-Sardos, Helene Pailhories, Carole Lemarie, Hanaa Benmansour, Camille D'Humieres, Lauranne Broutin, Olivier Dauwalder, Michael Levy, Gabriel Auger, Solen Kerneis, Vincent Cattoir
Summary: The study evaluated the performance of FA-PP in detecting bacterial pathogens and antibiotic resistance genes in respiratory specimens, showing that it performed well in detecting pathogens in most specimens. The positive and negative percentage agreement values of FA-PP compared to culture were high, but there were some false-negative results. In addition, there was some discordance between the semi-quantification results of FA-PP and culture results.
CLINICAL MICROBIOLOGY AND INFECTION
(2021)
Article
Dentistry, Oral Surgery & Medicine
Xinling Wang, Yuejun Tang, Rui Xiao
Summary: This study found that levels of chemerin and inflammatory cytokines were higher in the gingival crevicular fluid and serum of chronic periodontitis (CP) patients, and were positively correlated with each other. Periodontal ligament stem cells (PDLSCs) isolated from CP patients had higher expressions of chemerin and these cytokines compared to healthy individuals. Furthermore, chemerin dose-dependently increased inflammatory responses and inhibited osteogenic differentiation of PDLSCs.
Article
Energy & Fuels
Li-Hui Hao, Chang-Qiao Chi, Na Luo, Yong Nie, Yue-Qin Tang, Xiao-Lei Wu
Summary: The study demonstrates the potential of utilizing indigenous oil-phase microbes for microbial enhanced oil recovery (MEOR). Experimental and field trials showed that the synthetic microbial consortium K can significantly improve oil recovery and be effective in extremely viscous oil reservoirs with high water-cut.
PETROLEUM SCIENCE AND TECHNOLOGY
(2023)
Article
Chemistry, Applied
Jie Ding, Liu Dong, Pengfei Jiang, Yue Tang, Songyi Lin
Summary: Enzymatic hydrolysis is an effective method to reduce the allergenicity of pea protein, and this study explored the relationship between reduced allergenicity and structural changes.
Article
Agricultural Engineering
Shi-Peng Wang, Xiao-Xing Li, Zhao -Yong Sun, Wen-Liang Shuai, Zi-Yuan Xia, Cai-Yun Xie, Min Gou, Yue-Qin Tang
Summary: This study evaluated the compostability of rice straw with different nitrogen-rich wastes and compared their effects on organic matter degradation, maturity, fertility, and bacterial community structure. The addition of food waste was found to be the most effective in accelerating rice straw degradation and improving end-product fertility. The succession of the bacterial community was also enhanced with food waste supplementation. This study provides a promising alternative method for the disposal of a larger amount of rice straw in a shorter time.
BIORESOURCE TECHNOLOGY
(2023)
Article
Microbiology
Liyun An, YingChun Yan, Yong Nie, Min Gou, YueQin Tang, Xiao-Lei Wu
Summary: Microbes can use both organic and inorganic compounds as electron donors to produce energy for growth. Previous studies have mainly focused on the effects of different electron donors on microbial community under a set cathode potential, while their response to different redox potentials in different environments remains under-researched. In this study, lake sediment was incubated in a reactor equipped with three working electrodes at different potentials. The results showed that the structure of bacterial communities was similar for closed-circuit electrodes, but significantly different for open-circuit electrodes. Specific bacteria were also preferentially enriched by different electrode potentials.
CURRENT MICROBIOLOGY
(2023)
Article
Environmental Sciences
Zong-Lin Wu, Quan Zhang, Zi-Yuan Xia, Min Gou, Zhao-Yong Sun, Yue-Qin Tang
Summary: This study investigated the effects of fluctuation disturbance of organic loading rate (OLR) on anaerobic digestion (AD) of municipal sludge. The performance of the AD reactors remained similar before and after disturbance, indicating their buffer capacity to OLR periodic fluctuation. Different microbial community at RNA level was observed in the two reactors, with greater changes in the thermophilic AD. The results showed that the thermophilic microbial community was more vulnerable, but the reactor performance could be maintained using the functional redundancy strategy under OLR fluctuation disturbance.
ENVIRONMENTAL RESEARCH
(2023)
Article
Engineering, Environmental
Yue Yi, Jan Dolfing, Ge Jin, XiaoYu Fang, WenHao Han, LaiYan Liu, YueQin Tang, Lei Cheng
Summary: In this study, the influence of ammonia on different methanogenic pathways was evaluated by culturing Methanosarcina barkeri with acetate, H2/CO2, and methanol. Aceticlastic methanogenesis was more sensitive to increased ammonia concentrations than hydrogenotrophic and methylotrophic methanogenesis. The results reveal the varying ammonia tolerances of M. barkeri under different methanogenic pathways, providing insights into the role of the thermodynamic potential of methanogenesis in ammonia stress tolerance and shedding light on the mechanism of ammonia inhibition on anaerobic digestion.
Article
Cell Biology
Yan Tang, Li-Jie Yang, Hao Liu, Yan-Jue Song, Qi-Qi Yang, Yang Liu, Shu-Wen Qian, Qi-Qun Tang
Summary: Obesity, especially increased visceral fat, is associated with metabolic challenges such as atherosclerosis, but the exact mechanism is not fully understood. This study aims to investigate the role of visceral-fat-derived exosomes in endothelial cells and atherosclerosis. The research shows that obesity alters the miRNA profile of adipose exosomes, and exosomal miR-27b-3p can enter endothelial cells and activate the NF-kB pathway by suppressing PPARa. In ApoE-deficient mice, administration of miR-27b-3p mimic increases inflammation and atherogenesis, while overexpression of PPARa protects against atherosclerosis. Therefore, obesity-induced exosomal miR-27b-3p promotes endothelial inflammation and facilitates atherogenesis through PPARa suppression.
Article
Microbiology
Gao Feng, Yan Zeng, Hui-Zhong Wang, Ya-Ting Chen, Yue-Qin Tang
Summary: Microorganisms in anaerobic digestion are affected by ammonia, especially methanogens. This study investigated the effect of ammonia on microbial community and metabolism in a mesophilic chemostat. Results showed that even at high ammonia concentrations, methanogenesis and acetate degradation remained normal. The dominant methanogen changed with increased ammonia, and Proteiniphilum showed a positive correlation with ammonia concentration. Proteiniphilum and M. harundinacea had strong ammonia tolerance and played critical roles in acetate degradation. These findings contribute to the regulation and management of anaerobic digestion processes.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Agricultural Engineering
Xiang-Yu Peng, Shi-Peng Wang, Xiu-Lin Chu, Zhao-Yong Sun, Zi-Yuan Xia, Cai-Yun Xie, Min Gou, Yue-Qin Tang
Summary: This study investigated the performance and bacterial community succession of a thermophilic semi-continuous composting (TSC) followed by static stacking (SS) for decentralized kitchen waste (KW) treatment. The TSC using an integrated automatic reactor achieved a stable performance with an organic matter degradation efficiency of 80.88% and a seed germination index (GI) of 78.51% by a daily feeding ratio of 10%. The subsequent SS for seven days further improved the compost quality with a GI of 91.58% by promoting the rapid proliferation of Proteobacteria and Bacteroidetes. The results offer new perspectives for practical applications of decentralized KW treatment using TSC.
BIORESOURCE TECHNOLOGY
(2023)
Article
Physics, Multidisciplinary
Haipeng Zhang, Jiajie Long, Li Ren, Ying Xu, Xianhao Li, Yuejin Tang
Summary: Developing a transient magneto-mechanical numerical model is crucial to accurately simulate the influence of deep-sea pressure on the magnetic field around underwater detectors. This study improved the Jiles-Atherton theory for complex stress and verified the hysteresis effect between magnetization and stress by calculating the variation in magnetic field with pressure. The effect of stress on magnetic field was determined by calculating the magnetic field distribution under different pressures.
Article
Urology & Nephrology
Licong Su, Yanqin Li, Ruixuan Chen, Xiaodong Zhang, Yue Cao, Fan Luo, Mingjing Pi, Ruqi Xu, Qi Gao, Shiyu Zhou, Ying Hu, Hua Li, Qiongqiong Yang, Qijun Wan, Bicheng Liu, Hong Xu, Guisen Li, Jianping Weng, Gang Xu, Chunbo Chen, Huafeng Liu, Yongjun Shi, Yan Zha, Yaozhong Kong, Guobin Su, Ying Tang, Yilun Zhou, Mengchun Gong, Xin Xu, Sheng Nie
Summary: Acute kidney injury (AKI) is associated with increased risks of new-onset and worsening proteinuria. A large multicenter cohort study was conducted to evaluate the incidence, risk factors and clinical correlations of post-AKI proteinuria among hospitalized patients. It was found that post-AKI proteinuria is common and usually transient among hospitalized patients. The risk profiles for new-onset and worsening proteinuria differed markedly. Worsening proteinuria after AKI was associated with adverse kidney outcomes, highlighting the need for close monitoring.
CLINICAL KIDNEY JOURNAL
(2023)
Article
Immunology
Sha Lu, Zi Li, Jinquan Wu, Ying Tang, Qianling Zhang, Liyan Xi
Summary: This study investigates the role of the NLRP3 inflammasome in Talaromyces marneffei infection. The results show that this pathway is activated in infected mice and macrophages, leading to the release of IL-1β and contributing to early-stage mortality in pulmonary infection.
MICROBIAL PATHOGENESIS
(2023)
Article
Microbiology
Yuesong Weng, Tao Wang, Bin Huang, Hua Yu, Wei Jia, Bin Shan, Fen Qu, Yiwei Tang, Liang Chen, Hong Du
Summary: This study investigated the extent of colistin heteroresistance among CRKP strains in China, revealing a prevalence of 6.2%. It was found that the ST11 clone accounted for 60.7% of the colistin-heteroresistant isolates. The study also showed that heteroresistance could be suppressed by an efflux pump inhibitor and identified the significant role of the PhoPQ pathway in the mechanisms of heteroresistance.
MICROBIOLOGY SPECTRUM
(2023)
Article
Integrative & Complementary Medicine
Xuan Long, Kai Sun, Kai Wang, Lishuai Shi, Limin Cao, Tingting Wen, Zhonghua Zhou, Zhihua Guo, Yue Shi, Hong Yu, Zhen Yu, Xiaolian Song, Lijin Feng, Aihong Mei, Ning Xu
Summary: This study aimed to explore the differences between nonmedical and medical college students in their cognitive interest, preventive behaviors, psychological effects, and job-search intentions during the COVID-19 epidemic. The findings showed that medical students had higher interest in COVID-19, while nonmedical students exhibited stronger protective behaviors. In addition, COVID-19 had a significant impact on medical students' lives, studies, and moods, as well as their job-search intentions.
ALTERNATIVE THERAPIES IN HEALTH AND MEDICINE
(2023)
