期刊
JOURNAL OF CLINICAL INVESTIGATION
卷 126, 期 9, 页码 3403-3416出版社
AMER SOC CLINICAL INVESTIGATION INC
DOI: 10.1172/JCI85624
关键词
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资金
- US Public Health Service Grants [HL67724, HL91469, HL102738, HL112330, AG23039]
- Leducq Foundation Transatlantic Network of Excellence
- American Heart Association
- Banyu Foundation Research Grant
- Takeda Science Foundation
- Sakakibara Memorial Research Grant
- Grants-in-Aid for Scientific Research [15K19358, 15H04815] Funding Source: KAKEN
NADPH oxidases (Noxes) produce ROS that regulate cell growth and death. NOX4 expression in cardiomyocytes (CMs) plays an important role in cardiac remodeling and injury, but the posttranslational mechanisms that modulate this enzyme are poorly understood. Here, we determined that FYN, a Src family tyrosine kinase, interacts with the C-terminal domain of NOX4. FYN and NOX4 colocalized in perinuclear mitochondria, ER, and nuclear fractions in CMs, and FYN expression negatively regulated NOX4-induced O-2(-) production and apoptosis in CMs. Mechanistically, we found that direct phosphorylation of tyrosine 566 on NOX4 was critical for this FYN-mediated negative regulation. Transverse aortic constriction activated FYN in the left ventricle (LV), and FYN-deficient mice displayed exacerbated cardiac hypertrophy and dysfunction and increased ROS production and apoptosis. Deletion of Nox4 rescued the exaggerated LV remodeling in FYN-deficient mice. Furthermore, FYN expression was markedly decreased in failing human hearts, corroborating its role as a regulator of cardiac cell death and ROS production. In conclusion, FYN is activated by oxidative stress and serves as a negative feedback regulator of NOX4 in CMs during cardiac remodeling.
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