期刊
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
卷 1033, 期 -, 页码 40-48出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2016.08.005
关键词
Deep eutectic solvent; Negative pressure cavitation-assisted extraction; Dalbergia odorifera T. Chen; Isoflavonoids; Macroporous resin column chromatography
资金
- National Key Research Development Program of China [2016YFD0600805]
- Application Technology Research and Development Program of Harbin [2013AA3BS014]
- Fundamental Research Funds for the Central Universities [2572015EA04]
- Special Fund of National Natural Science Foundation of China [31270618]
In this study, green and efficient deep eutectic solvent-based negative pressure cavitation-assisted extraction (DES-NPCE) followed by macroporous resin column chromatography was developed to extract and separate four main isoflavonoids, i.e. prunetin, tectorigenin, genistein and biochanin A from Dalbergia odorifera T. Chen leaves. The extraction procedure was optimized systematically by single-factor experiments and a Box-Behnken experimental design combined with response surface methodology. The maximum extraction yields of prunetin, tectorigenin, genistein and biochanin A reached 1.204, 1.057, 0.911 and 2.448 mg/g dry weight, respectively. Moreover, the direct enrichment and separation of four isoflavonoids in DES extraction solution was successfully achieved by macroporous resin AB-8 with recovery yields of more than 80%. The present study provides a convenient and efficient method for the green extraction and preparative separation of active compounds from plants. (C) 2016 Elsevier B.V. All rights reserved.
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