Article
Biochemical Research Methods
Michaela Rektorisova, Vojtech Hrbek, Monika Tomaniova, Petr Cuhra, Jana Hajslova
Summary: The use of ultra-high performance supercritical fluid chromatography coupled to quadrupole-time of flight mass spectrometry (UHPSFC-QTOF-MS) as an alternative method for assessing chocolate quality and authenticity was investigated. The results showed that this method is fast, cost-effective, and efficient, providing a comprehensive analysis of sample composition.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Martin Meyer, Lidia Montero, Sven W. Meckelmann, Oliver J. Schmitz
Summary: This study compared the analysis of carbohydrates using four common chromatographic methods coupled to mass spectrometry. It was found that gas chromatography and reversed-phase liquid chromatography, after derivatisation, showed superior separation performance compared to the other methods. Targeted analysis using different working modes of the mass spectrometer improved sensitivity and repeatability, with RP-LC-MS in MRM mode after derivatisation being the most suitable method for monosaccharide analysis.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Michael Zwerger, Markus Ganzera
Summary: Boswellic acids, bioactive constituents in Indian frankincense, were successfully separated and quantified in B. serrata extracts using Supercritical Fluid Chromatography for the first time. The method showed excellent performance in terms of accuracy, precision, and sensitivity, with potential for application in analyzing commercial products with variable concentrations of boswellic acids.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Environmental Sciences
Chao Guo, Shanshan Di, Xiaolong Chen, Yanhua Wang, Peipei Qi, Zhiwei Wang, Huiyu Zhao, Yuanlin Gu, Hao Xu, Yuele Lu, Xinquan Wang
Summary: This study evaluated the enantioselectivity, degradation, and residue of chiral triticonazole in three kinds of fruits. The results showed that the degradation and residue concentrations varied among different fruits. Additionally, the dietary intake risks of triticonazole in fruits were found to be low in different age and gender groups.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Chemistry, Applied
Ziyang Diao, Shanshan Di, Peipei Qi, Zhenzhen Liu, Zhiwei Wang, Huiyu Zhao, Meng Wang, Chenghui Zhang, Xinquan Wang
Summary: This study summarized the stereoselective behaviors of Metconazole in four fruits and developed a new chiral separation method. The results of this study are significant for the comprehensive understanding of chiral studies and risk assessments of Metconazole.
Article
Chemistry, Analytical
Toshinobu Hondo, Chihiro Ota, Yumi Miyake, Hiroshi Furutani, Michisato Toyoda
Summary: PTR-MS is a real-time method used to detect trace VOCs in gaseous samples and is popular in various research fields. Supercritical fluid chromatography offers high separation efficiency and easy optimization. Combining PTR with SFC-MS interface allows for detection of nonvolatile organic compounds.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Physical
Rafael Hernandez-Tenorio, Aracely Hernandez-Ramirez, Monika Moeder, Jorge Luis Guzman-Mar
Summary: This study investigated the removal of oxcarbazepine from water through photolysis and heterogeneous photocatalysis processes, and monitored the formation of transformation products. The results showed that oxcarbazepine was completely degraded within 30 minutes using both methods.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
(2022)
Article
Chemistry, Analytical
Hidetoh Toki, Jun-ichi Yamaguchi, Akiko Mizuno-Yasuhira, Hiromi Endo
Summary: A convenient LC-MS/MS assay method was developed to simultaneously determine the enantiomers of (R,S)-ketamine, (R,S)-norketamine, and (2R,6R;2S,6S)-hydroxynorketamine in mice plasma and brain. This method allows for chiral separations of these enantiomers in a short analysis time using a column-switching system. The accuracy and linearity of the calibration curves showed promising results, and this method was successfully applied to determine the pharmacokinetic profiles of these enantiomers in plasma and brain samples collected from mice. Understanding the relationship between the antidepressant actions and pharmacokinetics of these enantiomers is essential.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Pharmacology & Pharmacy
Sanghee Choi, Wang-Seob Shim, Jiyoung Yoon, Doowon Choi, Jinseong Lee, Soo-Heui Paik, Eun-Kyoung Chung, Kyung-Tae Lee
Summary: The purpose of this study was to develop a method for simultaneously separating ibuprofen enantiomers using electrospray ionization liquid chromatography with tandem mass spectrometry. The method involved negative ionization and multiple reaction monitoring modes, with specific transitions monitored for each component. The developed method was fully validated and complied with regulatory guidelines. The validated assay was then applied to pharmacokinetic studies in beagle dogs.
Article
Chemistry, Inorganic & Nuclear
Tomoaki Tanase, Kanako Nakamae, Suzui Hayashi, Ayumi Okue, Risa Otaki, Tomoko Nishida, Yasuyuki Ura, Yasutaka Kitagawa, Takayuki Nakajima
Summary: Binuclear Pd(II) and Pt(II) complexes supported by rac-dpmppm were synthesized with isocyanide ligands, leading to the formation of tetranuclear complexes. Optical resolution of rac-dpmppm was achieved, and chiral octapalladium chains were synthesized and characterized, representing the longest enantiopure chiral single-metal-atom chains structurally characterized to date.
INORGANIC CHEMISTRY
(2021)
Article
Chemistry, Analytical
Sandra Salido-Fortuna, Maria Luisa Marina, Maria Castro-Puyana
Summary: This study developed a chiral selective method using Electrokinetic Chromatography for the simultaneous separation of licarbazepine acetate and licarbazepine. The method effectively separated the four enantiomers in a short time and showed suitability for various applications.
Article
Biochemical Research Methods
Randi R. Carter, Alexander L. Kovach, Brian F. Thomas
Summary: This study utilized the UPC2-MS2 method to detect and quantify nicotine and minor alkaloids in NRC tobacco products. Compared to other methods, UPC2-MS2 demonstrated higher sensitivity by accurately detecting every analyte in every NRC formulation.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Ana M. Ares, Jose Bernal, Andrea Janvier, Laura Toribio
Summary: This study investigated the separation of ten flavanones using supercritical fluid chromatography, focusing on achiral and chiral approaches. The best results for achiral separation were achieved using the Lichrospher 100 Diol column, while the chiral column Chiralpak AD was utilized for separating enantiomers. The proposed chiral method was successfully applied to analyze bee pollen samples.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biochemical Research Methods
Ernst Freund, Daniel Meyer, Nadine Schneider, Marie-Anne Lozac'h, Harald Schroder, Cagatay Cinar, Gotz Schlotterbeck, Trixie Wagner
Summary: An automated screening process for chiral supercritical fluid chromatography methods was established, resulting in increased efficiency and reliability of lab processes. Statistical analysis showed that up to 92% of tested racemic mixtures could be successfully separated, highlighting the usefulness of consistent chromatographic data sets for identifying chiral separation methods.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Analytical
Xiaolan Xu, Yiqin Lv, Kuanjin Tang, Bin Song, Qikun Jiang, Lingling Sun, Zhonggui He, Tianhong Zhang
Summary: A valine carbamate prodrug of naringenin was successfully synthesized in this study, and a sensitive SFC-MS/MS method was developed for evaluating their pharmacokinetic behavior. The results showed higher bioavailability of naringenin in the prodrug group compared to the naringenin suspension group.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Medicine, Research & Experimental
Yudi Wang, Jiawen Chen, Xiao Liang, Haobo Han, Hao Wang, Yan Yang, Quanshun Li
MOLECULAR PHARMACEUTICS
(2017)
Article
Biochemistry & Molecular Biology
Zhongyu Zhang, Yi Zheng, Hao Wang, Yifa Zhou, Guihua Tai
Article
Biochemistry & Molecular Biology
Jiyong Su, Linlin Cui, Yunlong Si, Chenyang Song, Yuying Li, Tong Yang, Hao Wang, Kevin H. Mayo, Guihua Tai, Yifa Zhou
BIOSCIENCE REPORTS
(2018)
Article
Plant Sciences
Lei Han, Chaoxia Xiao, Binbin Xiao, Meng Wang, Jingtong Liu, Nadeem Bhanbhro, Adnan Khan, Hao Wang, Huan Wang, Chunwu Yang
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2019)
Article
Chemistry, Analytical
Tingting Wang, Lei Yin, Hao Wang, John Paul Fawcett, Jingkai Gu
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2019)
Article
Biochemistry & Molecular Biology
Yunlong Si, Xing Wang, Guosong Yang, Tong Yang, Yuying Li, Gabriela Jaramillo Ayala, Xumin Li, Hao Wang, Jiyong Su
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Chemistry, Analytical
Hao Wang, Mi Zheng, Jingyi Gao, Jing Wang, Qi Zhang, J. Paul Fawcett, Yang He, Gu Jingkai
Article
Microbiology
Yuying Li, Yuan Yao, Guosong Yang, Jun Tang, Gabriela Jaramillo Ayala, Xumin Li, Wenlu Zhang, Qiuyu Han, Tong Yang, Hao Wang, Kevin H. Mayo, Jiyong Su
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Materials Science, Biomaterials
Wei Guo, Yongjie Wang, Pengqi Wan, Hao Wang, Li Chen, Shaokun Zhang, Chunsheng Xiao, Xuesi Chen
Summary: Bacterial infections and antibiotic resistance have become a global healthcare crisis. Cationic amphiphilic dendrons designed and synthesized showed excellent antimicrobial activities, depending highly on the length of the hydrophobic alkyl chain. The identified prime candidate exhibited rapid and broad-spectrum bactericidal activity, disrupting the integrity of a pathogen's membrane and promoting wound healing.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
Article
Materials Science, Biomaterials
Wei Guo, Wenchang Liu, Pengqi Wan, Hao Wang, Chunsheng Xiao, Li Chen, Xuesi Chen
Summary: This study reports on the potential of cationic amphiphilic dendrons with different hydrophobic alkyl chains and generations for anticancer applications. A specific dendron, C12-G1, showed broad-spectrum antitumor activity with negligible hemolysis. Mechanistic studies revealed that C12-G1 induced cancer cell necrosis through membrane disruption and inhibited tumor growth in a melanoma model.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2022)
Article
Plant Sciences
Hao Wang, Yantong Sun, Wei Guo, Jing Wang, Jingyi Gao, Wenwen Peng, Jingkai Gu
Summary: This study investigated the metabolism and pharmacokinetics of baicalein in humans, finding that baicalein is extensively metabolized in the body and identifying seven metabolites. The study established a high-throughput method for quantifying baicalein and its metabolites and provided a foundation for the potential application of baicalein in medicine.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Chemistry, Applied
Liming Guo, Hao Wang, Mengjuan Li, Xiaoxuan Xiang, Chensheng Xiao, Xi Lin, Xinhua Guo
Summary: Accurate and rapid quantification of GSH is crucial in wine quality control. In this study, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was demonstrated to be a high-throughput method for quantification of GSH and GSSG in wines, showing its feasibility and potential for wider applications.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Article
Engineering, Biomedical
Weiquan Gong, Tianhui Zhang, Mingxue Che, Yongjie Wang, Chuanyu He, Lidi Liu, Zhenshan Lv, Chunsheng Xiao, Hao Wang, Shaokun Zhang
Summary: Spinal cord injuries (SCIs) are devastating, leading to permanent loss of nerve function and paralysis. Current clinical treatments have limitations, and nanomaterials offer new possibilities for SCI treatment. Nanomaterials can improve the microenvironment of traumatic injury, promote neuron regeneration, optimize drug effects, and enhance gene delivery efficiency. This review provides new perspectives for future clinical therapies.
MATERIALS TODAY BIO
(2023)
Article
Materials Science, Multidisciplinary
Yixuan Liu, Feng Lin, Tianhui Zhang, Cheng Wu, Wanguo Liu, Hao Wang, Chunsheng Xiao, Xuesi Chen
Summary: Neuroprotective therapy for traumatic spinal cord injury (SCI) still faces challenges, and the toxic reactive aldehydes produced immediately after SCI promote oxidative stress and inflammation, worsening the secondary injury. To address this, a drug-free polypeptide (PPAH) was designed and synthesized to scavenge toxic aldehydes and mitigate secondary SCI. In vitro and in vivo experiments demonstrated PPAH's ability to scavenge reactive aldehydes, protect against damage in neurocytes, and improve functional recovery in a rat SCI model. PPAH shows promise as an effective therapy for SCI.
SCIENCE CHINA-MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Hao Wang, Feng Lin, Yi Wu, Wei Guo, Xuesi Chen, Chunsheng Xiao, Meiwan Chen
Summary: Spinal cord injury (SCI) is a severe condition that hinders neurological functional recovery and leads to permanent disabilities. Methylprednisolone (MP) is commonly used for SCI treatment, but it has potential side effects. In this study, we developed carrier-free nanoparticles (MP2-TK@RU NPs) that combine MP dimers and rutin to improve SCI treatment. The MP2-TK@RU NPs demonstrated anti-inflammatory and antioxidative properties, promoting locomotor function recovery and neuroprotection in rats with SCI. This carrier-free nanodrug shows promise as a therapeutic strategy for SCI treatment.