Article
Biochemical Research Methods
P. Jake Violi, P. David Bishop, P. Matthew Padula, T. Mika Westerhausen, J. Kenneth Rodgers
Summary: Developing LC-MS/MS methods for detecting native amino acids can be challenging, leading many scientists to derivatise amino acids to improve sensitivity. Derivatisation offers benefits such as reduced signal-to-noise ratios and more efficient ionisation, but it can also be costly and time-consuming. This study aimed to enhance the detection sensitivity of 20 protein amino acids using HILIC-MS/MS.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Review
Plant Sciences
Xiuli Han, Yongqing Yang
Summary: High salinity threatens crop production by harming plants and interfering with their development. Phospholipids regulate salt stress response by participating in salt stress signal transduction. Different phospholipid compounds play important roles in plant cell response to salt stress.
Article
Biochemical Research Methods
Giorgis Isaac, Ian D. Wilson, Robert S. Plumb
Summary: This study investigated the application of hybrid surface technology (HST) on the metal surfaces of an ACQUITYTM UPLCTM system and column to mitigate the issues of chelation, poor peak shape, and analyte loss caused by acidic phospholipids. The results showed significant improvements in sensitivity, recovery, and peak shape when using HST for the analysis of various phospholipids. The HST system and column particularly improved chromatographic performance at low analyte concentrations. The application of HST also led to reduced peak tailing, narrower peak widths, and increased signal intensities. Similar improvements in data quality were observed when analyzing chicken egg phosphatidic acid (PA) and brain porcine phosphatidylserine (PS) extracts.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Organic
Athanasios Papangelis, Trond Ulven
Summary: The study presents a highly selective method for synthesizing LPC and mixed PC without the need for chromatography. By synthesizing an intermediate and using TFA in aqueous solution to remove the protecting group, the synthesis of pure LPC was achieved.
JOURNAL OF ORGANIC CHEMISTRY
(2022)
Article
Chemistry, Applied
Yan Tang, Xuechun Sun, Haiyue Hou, Augustine Atta Debrah, Mengyu Wang, Fengxia Zhang, Zhenxia Du
Summary: A new method was developed in this study to investigate the impact of different processing techniques on the composition of phospholipids. The composition of phospholipids underwent minor changes after pasteurization, while the spray-drying process significantly affected the concentration of phospholipids, especially certain types.
Article
Biochemical Research Methods
Pauline Bosman, Valerie Pichon, Ana Carolina Acevedo, Helene Chardin, Audrey Combes
Summary: Advancements in metabolomics have allowed for the identification and characterization of saliva metabolites as biomarkers, but discrepancies in biomarker content for a given disease have been observed. Differences in saliva collection, storage, and treatment methods could contribute to these discrepancies, as there is currently no standardized method. This study investigated the effects of different collection methods on saliva metabolite analysis, and found that saliva obtained through spitting and aspiration yielded comparable results, while the Salivette (R) method did not appear suitable for untargeted analysis of the salivary metabolome.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Mayssa Hachem, Houda Nacir
Summary: This review focuses on the targeted delivery of DHA to the brain in different lipid forms and suggests future research directions. DHA is essential for neural development, learning, and vision, but deficiencies in cerebral DHA levels have been associated with neurodegenerative diseases. Understanding the role of DHA in brain transport and its beneficial effects in healthy conditions and brain disorders is crucial for the prevention and treatment of neurodegenerative diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Johanna Hiefner, Johann Rische, Madeleine J. Bunders, Anna Worthmann
Summary: This study presents a method based on HPLC-MS/MS for the quantification of adenine nucleotides, which helps us understand the impact of disturbances in AN balance on cellular function.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Chemistry, Analytical
Lanyu Liu, Qian Liu, Yan Wang, Yu Yuan, Feifan Xie
Summary: This study aimed to evaluate different combinations of polyanion-metal ion based solution system for phospholipids removal and elimination of matrix effects in human plasma. The optimized ZrOCl2-Ludox/DSS systems provided efficient phospholipids removal and adequate analyte recovery, and they also correctly eliminated ion suppression or enhancement of the non-polar and polar drugs.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biochemistry & Molecular Biology
John Quinones, Rommy Diaz, Jorge F. Beltran, Lidiana Velazquez, David Cancino, Erwin Munoz, Patricio Dantagnan, Adrian Hernandez, Nestor Sepulveda, Jorge G. Farias
Summary: This study analyzed the effects of different feeds on the lipid composition of juvenile Oncorhynchus mykiss muscle and found that the inclusion of Brassica napus oil significantly increased certain lipid levels, while Durvillaea antarctica meal had a less significant impact on lipid variation.
Article
Chemistry, Analytical
Xiaona Li, Zhini Liu, Zhuo Li, Xin Xiong, Xianhua Zhang, Changqing Yang, Libo Zhao, Rongsheng Zhao
Summary: A novel LC-MS method was developed for simultaneous quantification of 16 purine metabolites in human plasma and urine. The method showed good linearity, low quantitation limits, and stable recovery and matrix effects. The method was successfully applied to compare the plasma and urine samples of patients without and with acute kidney injury (AKI), revealing potential upregulation of certain purine intermediates in AKI patients, indicating an association with inflammatory responses.
Article
Chemistry, Analytical
Jose Fernando Huertas-Perez, Pascal Mottier, Quentin Basle, Shi Ying Tan, Monika Kopec-Durska, Patrycja Zawada, Antoinette Burton, Ashley Griffin, Maria Guadalupe Sanchez-Calderon
Summary: This study evaluated an analytical procedure for routine monitoring of chlorate and perchlorate in a broad range of food matrices, using liquid chromatography tandem mass spectrometry. The method demonstrated high accuracy and is suitable for monitoring in various food matrices.
MICROCHEMICAL JOURNAL
(2022)
Review
Biochemistry & Molecular Biology
Beatriz A. Rodas-Junco, Graciela E. Racagni-Di-Palma, Michel Canul-Chan, Javier Usorach, S. M. Teresa Hernandez-Sotomayor
Summary: Plants have developed mechanisms to process extracellular signals through transient molecules known as second messengers, which can modulate physiological responses and gene expression under stress conditions. Recent research has focused on the molecular mechanisms of lipid second messengers in response to osmotic stress, highlighting their ability to recruit target proteins to the membrane and affect protein function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Applied
Zhengcao Xiao, Xiaohui Hou, Ting Zhang, Yahong Yuan, Jianbo Xiao, Wei Song, Tianli Yue
Summary: An accurate HILIC-MS method was used to characterize starch digestion and measure the inhibition properties of flavonoids against alpha-amylase. Glucose oligosaccharide products with different degrees of polymerization were identified, with products of DPs 2, 3, 6, 7, and 9 showing higher yields, and DP 9 having the highest yields. Pelargonidin exhibited the best inhibition activity on all enzyme products.
Article
Biochemistry & Molecular Biology
Dmitri Pismennoi, Vassili Kiritsenko, Jaroslav Marhivka, Mary-Liis Kutt, Raivo Vilu
Summary: A method for quantifying five saccharides in fermentation samples produced by Streptococcus thermophilus was developed, allowing for accurate and high-throughput analysis.
Letter
Hematology
Meletios A. Dimopoulos, Philippe Moreau, Bradley Augustson, Nelson Castro, Tomas Pika, Sosana Delimpasi, Javier De la Rubia, Angelo Maiolino, Tony Reiman, Joaquin Martinez-Lopez, Thomas Martin, Joseph Mikhael, Kwee Yong, Marie-Laure Risse, Gaelle Asset, Sylvia Marion, Roman Hajek
AMERICAN JOURNAL OF HEMATOLOGY
(2023)
Article
Oncology
Vladimir Maisnar, Ludek Pour, Ivan Spicka, Tomas Jelinek, Jiri Minarik, Alexandra Jungova, Martin Stork, Jan Straub, Jakub Radocha, Tomas Pika, Lenka Pospisilova, Sandhya Nair, Peter Kunovszki, Roman Hajek
Summary: A study analyzed data from the Czech Registry of Monoclonal Gammopathies and found that among 83 triple-class exposed relapsed/refractory multiple myeloma patients, the median overall survival was 14.2 months, progression-free survival was 6.2 months, and time to next treatment was 7.2 months. These results indicate a high disease burden in this patient population, consistent with previous studies.
CLINICAL LYMPHOMA MYELOMA & LEUKEMIA
(2023)
Article
Oncology
Michael A. Thompson, Mario Boccadoro, Xavier Leleu, Jorge Vela-Ojeda, Frits van Rhee, Katja C. Weisel, Robert M. Rifkin, Saad Z. Usmani, Roman Hajek, Gordon Cook, Rafat Abonour, Mira Armour, Kathryn E. Morgan, Su-Peng Yeh, Caitlin L. Costello, Jesus G. Berdeja, Faith E. Davies, Jeffrey A. Zonder, Hans C. Lee, Jim Omel, Andrew Spencer, Evangelos Terpos, Vania T. M. Hungria, Noemi Puig, Chengcheng Fu, Renda H. Ferrari, Kaili Ren, Dawn Marie Stull, Ajai Chari
Summary: We analyzed the vaccination rates of patients with multiple myeloma in the INSIGHT MM study and found that influenza and pneumococcal vaccination had an impact on overall survival. Additionally, vaccinated patients had fewer deaths due to infections compared to non-vaccinated patients. Recording vaccination status is important in prospective clinical trials as it may affect survival.
CLINICAL LYMPHOMA MYELOMA & LEUKEMIA
(2023)
Article
Oncology
Giovanni Palladini, Stefan Schonland, Giampaolo Merlini, Paolo Milani, Arnaud Jaccard, Frank Bridoux, Meletios A. Dimopoulos, Sriram Ravichandran, Ute Hegenbart, Wilfried Roeloffzen, M. Teresa Cibeira, Hermine Agis, Monique C. Minnema, Rui Bergantim, Roman Hajek, Cristina Joao, Alexandros Leonidakis, Giorgos Cheliotis, Pieter Sonneveld, Efstathios Kastritis, Ashutosh Wechalekar
Summary: This retrospective observational study in Europe examined the demographics, clinical characteristics, treatment patterns, and outcomes of 4480 systemic light chain (AL) amyloidosis patients who initiated first-line treatment from 2004-2018. The study found that bortezomib-based regimens were the most commonly used first-line therapy, and only a minority of patients received autologous stem cell transplant. The study highlighted the high early mortality rate and the lack of improvement in survival outcomes for the frail stage IIIb population despite the introduction of new therapies.
BLOOD CANCER JOURNAL
(2023)
Article
Oncology
Daisuke Ogiya, Zuzana Chyra, Sigitas J. Verselis, Morgan O'Keefe, Jacquelyn Cobb, Ivane Abiatari, Srikanth Talluri, Anjana Anilkumar Sithara, Teru Hideshima, Michael P. P. Chu, Roman Hajek, David M. Dorfman, Linda M. Pilarski, Kenneth C. Anderson, Sophia Adamia
Summary: This study found that alterations in pre-mRNA splicing (AS) are frequent in multiple myeloma (MM) patients, suggesting the need for innovative treatment strategies. The study also explores how to identify disease-specific alterations in MM and proposes RNA-based therapeutic strategies to eliminate these changes, demonstrating how aberrant HMMR splicing can be inhibited through RNA intervention.
BLOOD CANCER JOURNAL
(2023)
Article
Cell Biology
Sandra Charvatova, Benjamin Motais, Justyna Czapla, Tomasz Cichon, Ryszard Smolarczyk, Zuzana Walek, Sebastian Giebel, Roman Hajek, Juli R. Bago
Summary: We propose a novel approach for the local treatment of multiple myeloma bone disease (MBD) using a combination of natural killer cells (NKs) and mesenchymal stem cells (MSCs) within a fibrin scaffold, known as FINM. In vitro studies demonstrated that NKs within FINM exerted a robust anti-tumor activity against MM cells and suppressed osteoclast activity in a 3D model of MBD. The combination of FINM with other therapeutic agents showed a clear therapeutic synergistic effect.
Article
Chemistry, Analytical
Oleksandr Kozlov, Eliska Horakova, Sara Rademacherova, David Malinak, Rudolf Andrys, Eliska Prchalova, Miroslav Lisa
Summary: A fast and selective method using chiral supercritical fluid chromatography-mass spectrometry (SFC-MS) was developed to study lipase selectivity in the hydrolysis of triacylglycerols (TGs). The method involved the synthesis of enantiomerically pure monoacylglycerol (MG) and diacylglycerol (DG) isomers and their separation using a chiral column and neat methanol as a mobile phase modifier. The results revealed different stereoselectivities for substrates in the lipase-catalyzed hydrolysis.
ANALYTICAL CHEMISTRY
(2023)
Article
Oncology
Thomas Martin, Meletios-Athanasios Dimopoulos, Joseph Mikhael, Kwee Yong, Marcelo Capra, Thierry Facon, Roman Hajek, Ivan Spicka, Ross Baker, Kihyun Kim, Gracia Martinez, Chang-Ki Min, Ludek Pour, Xavier Leleu, Albert Oriol, Youngil Koh, Kenshi Suzuki, France Casca, Sandrine Mace, Marie-Laure Risse, Philippe Moreau
Summary: Longer-term outcomes of the combination treatment of isatuximab and carfilzomib-dexamethasone (Isa-Kd) were evaluated in a Phase 3 trial. The addition of Isa to Kd significantly prolonged progression-free survival and improved complete response rates and minimal residual disease negativity. The safety profile of Isa-Kd was similar to previous analyses. These findings support Isa-Kd as a standard-of-care treatment for relapsed multiple myeloma patients.
BLOOD CANCER JOURNAL
(2023)
Correction
Oncology
Thomas Martin, Meletios-Athanasios Dimopoulos, Joseph Mikhael, Kwee Yong, Marcelo Capra, Thierry Facon, Roman Hajek, Ivan Spicka, Ross Baker, Kihyun Kim, Gracia Martinez, Chang-Ki Min, Ludek Pour, Xavier Leleu, Albert Oriol, Youngil Koh, Kenshi Suzuki, France Casca, Sandrine Mace, Marie-Laure Risse, Philippe Moreau
BLOOD CANCER JOURNAL
(2023)
Meeting Abstract
Oncology
Maria-Victoria Mateos, Monika Szarejko, Jelena Bila, Fredrik Schjesvold, Ivan Spicka, Vladimir Maisnar, Artur Jurczyszyn, Zhanet Grudeva-Popova, Roman Hajek, Hanna Usenko, Marcus Thuresson, Stefan Norin, Sara Jarefors, Paul Richardson, Ludek Pour
CLINICAL LYMPHOMA MYELOMA & LEUKEMIA
(2023)
Meeting Abstract
Oncology
Enrique Ocio, Yvonne Efebera, Roman Hajek, Jan Straub, Vladimir Maisnar, Jean-Richard Eveillard, Lionel Karlin, Maria-Victoria Mateos, Albert Oriol, Vincent Ribrag, Paul Richardson, Stefan Norin, Jakob Obermuller, Nicolaas Bakker, Ludek Pour
CLINICAL LYMPHOMA MYELOMA & LEUKEMIA
(2023)
Meeting Abstract
Oncology
Jakub Radocha, Alexandra Jungova, Ludek Pour, Ivan Spicka, Jiri Minarik, Tomas Jelinek, Pavel Jindra, Martin Stork, Tomas Pika, Frantisek Sedlak, Tereza Popkova, Denisa Novakova, Michal Sykora, Petr Pavlicek, Adriana Heindorfer, Marek Wrobel, Evzen Gregora, Peter Mikula, Jarmila Obernauerova, Jana Ullrychova, Lukas Stejskal, Petr Kessler, Vladimir Maisnar, Roman Hajek
CLINICAL LYMPHOMA MYELOMA & LEUKEMIA
(2023)
Meeting Abstract
Oncology
Jakub Radocha, Ludek Pour, Tomas Jelinek, Jiri Minarik, Alexandra Jungova, Denisa Novakova, Martin Stork, Ludmila Muronova, Tomas Pika, Roman Hajek
CLINICAL LYMPHOMA MYELOMA & LEUKEMIA
(2023)
Meeting Abstract
Oncology
Kwee Yong, Thomas Martin, Meletios Dimopoulos, Joseph Mikhael, Marcelo Capra, Thierry Facon, Roman Hajek, Ivan Spicka, Ross Baker, Kihyun Kim, Gracia Martinez, Chang-Ki Min, Ludek Pour, Xavier Leleu, Albert Oriol, Youngil Koh, Kenshi Suzuki, France Casca, Sandrine Mace, Marie-Laure Risse, Philippe Moreau
CLINICAL LYMPHOMA MYELOMA & LEUKEMIA
(2023)
Meeting Abstract
Oncology
Tomas Jelinek, David Zihala, Tereza Sevcikova, Anjana Anilkumar Sithara, Veronika Kapustova, Hana Sahinbegovic, Ondrej Venglar, Ludmila Muronova, Lucie Broskevicova, Daniel Bilek, Tereza Popkova, Hana Plonkova, Jan Vrana, Vladimir Zidlik, Pavel Hurnik, Martin Havel, Matous Hrdinka, Zuzana Chyra, Michal Simicek, Roman Hajek
CLINICAL LYMPHOMA MYELOMA & LEUKEMIA
(2023)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
