Article
Chemistry, Applied
Christina Kosma, Ourania L. Koloka, Triantafyllos A. Albanis, Ioannis K. Konstantinou
Summary: A sensitive and rapid method for the determination of multiclass pesticides in white and red wines was developed using QuEChERS extraction procedure and UHPLC-Orbitrap-MS. The method was optimized to improve recoveries and reduce co-extractives, and successfully applied to real samples, revealing relatively low levels of two selected fungicides.
Article
Food Science & Technology
Shizuka Saito-Shida, Satoru Nemoto, Hiroshi Akiyama
Summary: The study demonstrated the applicability of LC-QTOF-MS method for quantitative and confirmatory analysis of pesticide residues in cereal grains and legumes, with high signal intensities and accuracy, suitable for regulatory routine analysis.
Article
Chemistry, Analytical
Ying Zhang, Bei Gao, Luis Valdiviez, Chao Zhu, Tara Gallagher, Katrine Whiteson, Oliver Fiehn
Summary: Stable isotope tracers are commonly used with gas chromatography coupled to mass spectrometry for enzyme activity and metabolic pathway studies. In this research, three commercially available instruments were used to analyze samples from a stable isotope labeling study on Rothia mucilaginosa metabolism. The study found that all three GC-MS instruments can be used for stable isotope tracing studies, with high-resolution GC-QTOF MS offering additional capabilities in identifying the chemical structures of unknown compounds.
ANALYTICAL CHEMISTRY
(2021)
Article
Food Science & Technology
Xuan Liu, Yanhong Fan, Chi-Peng Chang, Chih-Kang Lo, Xu Wang
Summary: A multi-component analytical method for pesticide residue detection in paprika using UHPLC-Q-TOF/MS was developed and validated. The method was found to be suitable for screening and quantitative analysis of pesticide residues in paprika, with one sample exceeding the maximum residue levels.
FOOD ANALYTICAL METHODS
(2021)
Article
Chemistry, Analytical
Ke-Xin Yue, Han-Lin Li, Zi-Feng Pi, Feng-Rui Song, Ying-Ping Wang, Zhong-Ying Liu
Summary: In this study, an efficient screening method was established for the determination of 90 pesticides residues in Panax Ginseng. The accuracy of the method was verified and a total of 73 pesticides were found suitable for rapid analysis of pesticide residues in Ginseng. The study also identified several pesticides with relatively severe residues in Ginseng samples available on the market.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Biochemical Research Methods
Yunjia Yang, Yi Yang, Jing Zhang, Kai Yao, Jinyuan Liu, Bing Shao
Summary: In this study, a method for simultaneous quantification of eight allergens in red wine by UPLC-MS/MS was developed. The detection limits for milk allergens were shown to be 0.003-0.015μg/mL, and 0.1μg/mL for egg allergens. This economical and reliable method is suitable for routine analysis and allergen label management.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Chemistry, Analytical
Li Cheng, Fang Wang, Yuanxin Cao, Guihan Cai, Qisheng Wei, Shuyun Shi, Ying Guo
Summary: This study developed a bioreaction-HPLC-quadrupole-time-of-flight-MS/MS method to screen and identify bioactive polyphenols with alpha-glucosidase inhibitory and antioxidant activities from Prunella vulgaris L. A total of 20 bioactive polyphenols were identified, and molecular docking analysis showed their binding with alpha-glucosidase through hydrogen bonding and pi-pi stacking. Density functional theory calculations demonstrated their electron transport ability and chemical reactivity. This study provides a valuable strategy for the rapid discovery of bioactive compounds and offers scientific evidence for the further development and application of P. vulgaris L.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Chemistry, Analytical
Florent Penen, Rene Raave, Annemarie Kip, Sandra Heskamp, Per Malmberg
Summary: ToF-SIMS imaging is a quick and sensitive technique that can visualize HER2 specific radiotracers in ovarian and breast cancer xenograft tumor sections to reflect the heterogeneous distribution of trastuzumab.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Giovanni Caria, Baghdad Ouddane, Sopheak Net, Nicolas Proix, Claire Froger
Summary: Lack of control over the use of pesticides in agricultural soils in France. Developed a targeted method for analysis of pesticides in soils. Detected multiple pesticides in 40 soil samples, with DCPMU and epoxiconazole being detected in all soils.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY
(2023)
Review
Spectroscopy
Christopher J. Brais, Jaime Orejas Ibanez, Andrew J. Schwartz, Steven J. Ray
Summary: Time-of-flight mass spectrometry (TOFMS) is a powerful and simple approach in mass spectrometry, requiring continuous innovation and development. New instrumental technologies enable the application of TOFMS in chemical measurement, such as ion acceleration, multiplexed detection, and extension of ion flight time.
MASS SPECTROMETRY REVIEWS
(2021)
Article
Chemistry, Analytical
Jingyun Huang, Jinian Shu, Bo Yang, Yedong Guo, Zuojian Zhang, Kui Jiang, Zhen Li
Summary: A thermal desorption associative ionization time-of-flight mass spectrometer was developed for ultrasensitive detection of semi-volatile chemical warfare agents. The excited-state CH2Cl2-induced associative ionization method showed excellent sensitivity towards CWAs, improving LODs by 2-76 times compared to other methods. The experiment demonstrated potential for significantly enhancing CWA detection in the future.
Article
Agricultural Engineering
Zainab Syed, Monika Sogani, Anu Kumar, Jayana Rajvanshi, Gopesh Sharma, Kumar Sonu
Summary: This research focused on the biodegradation of synthetic estrogenic compound 17 alpha-ethinylestradiol (EE2) and compared its degradation efficiency and power output in suspension flasks and a Bioelectrochemical System (BES). The results showed that the BES had significantly higher EE2 removal rate and power density. The study also identified a potential pathway for EE2 degradation using the newly isolated strain Rhodopseudomonas palustris MDOC01, and detected important metabolites during the bioelectrochemical treatment process of EE2.
BIORESOURCE TECHNOLOGY
(2022)
Article
Chemistry, Applied
L. Perez-Mayan, M. Ramil, R. Cela, I. Rodriguez
Summary: The study utilized LC-MS/MS combined with online solid-phase extraction (SPE) strategy to detect potential pesticide residues in wine. The method showed high accuracy and provided good recovery rates for 90% of compounds under various parameter conditions.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2021)
Article
Biochemical Research Methods
B. Oro-Nolla, M. Dulsat-Masvidal, A. Bertolero, A. Lopez-Antia, S. Lacorte
Summary: This study develops an extraction and analytical methodology for PFAS in bird eggs and blood, and detects several PFAS compounds, including PFOS and PFOA. The developed method increases the scope of PFAS analysis and promotes the use of bird species as bioindicators of chemical pollution.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Environmental Sciences
Abimbola E. Oluwalana, Tendai Musvuugwa, Stephen T. Sikwila, Jeremia S. Sefadi, Albert Whata, Mathew M. Nindi, Nhamo Chaukura
Summary: This study investigated the occurrence of emerging contaminants in wastewater in the Northern Cape province of South Africa. The research found a wide variety of emerging contaminants in wastewater treatment plants, with most of them being recalcitrant to treatment processes. The presence of natural organic matter (NOM) was also observed in the wastewater samples. The study highlights the need for monitoring and quantifying emerging contaminants in wastewater treatment plants to mitigate their ecotoxicological risks.
ENVIRONMENTAL POLLUTION
(2022)
Article
Biochemical Research Methods
S. Trinanes, P. Rodriguez-Mier, C. Cobas, E. Sanchez, R. Phan-Tan-Luu, R. Cela
JOURNAL OF CHROMATOGRAPHY A
(2020)
Article
Biochemical Research Methods
L. Perez-Mayan, M. Cobo-Golpe, M. Ramil, R. Cela, I Rodriguez
JOURNAL OF CHROMATOGRAPHY A
(2020)
Article
Environmental Sciences
M. Cobo-Golpe, M. Ramil, R. Cela, I Rodriguez
Article
Biochemical Research Methods
G. Castro, L. Perez-Mayan, I Carpinteiro, M. Ramil, R. Cela, I Rodriguez
JOURNAL OF CHROMATOGRAPHY A
(2020)
Article
Environmental Sciences
G. Castro, M. Ramil, R. Cela, I Rodriguez
Summary: The combination of UV/free chlorine was found to significantly enhance the degradability of VALA compared to other treatments, and was more effective than UV/H2O2 at neutral pH. Three potential transformation products of VALA were identified by LC-QTOF-MS, and the formation of volatile DBPs was observed through gas chromatography.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Environmental Sciences
L. Perez-Mayan, M. Ramil, R. Cela, I Rodriguez
Article
Biochemical Research Methods
L. Perez-Mayan, M. Ramil, R. Cela, I. Rodriguez
Summary: Supercritical fluid chromatography (SFC) combined with mass spectrometry (MS) was used to determine five chiral fungicides in wine and vineyard soils. Different SFC parameters were thoroughly described for effective chiral separations and ionization efficiency. The study showed non-enantioselective processes for azolic fungicides in soil dissipation and transfer from vines to wines, while for acylalanine compounds, the application of metalaxyl to vines as racemate and the R-enantiomer were confirmed, with varying enantiomeric fractions in vineyard soils.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Biochemical Research Methods
M. Cobo-Golpe, J. Garcia-Martin, M. Ramil, R. Cela, I. Rodriguez
Summary: The study examined the quantitative determination of triclosan (TCS) using direct analysis in real time coupled to accurate mass spectrometry (DART-MS) in samples of increasing complexity. The results showed that under final selected conditions, TCS could be determined with an instrumental limit of quantification of 5 ng mL(-1) and a linear response range up to 1000 ng mL(-1).
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
R. Cela, S. Trinanes, C. Cobas
Summary: A new strategy has been developed for computer-assisted methods in reversed-phase liquid chromatographic separations, using latent spectral information in chromatogram raw data files. By utilizing multivariate curve resolution-alternating least squares (MCR-ALS), this technique allows estimation of true spectra for individual components, providing a general approach for chromatography laboratory tools. This strategy is applicable to both known and unknown sample compositions, with the main difference being the need for an initial spectrum discovery process for unknown mixtures.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Environmental Sciences
G. Castro, M. Ramil, R. Cela, I Rodriguez
Summary: This study investigated the presence of emerging pollutants in sewage sludge using mass spectrometry detection and UPLC technology, identifying 68 micropollutants and reporting semi-quantitative concentration data for 37 pollutants, with 10 pharmaceuticals highlighted as pollutants to be monitored in sludge.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Chemistry, Applied
L. Perez-Mayan, M. Ramil, R. Cela, I. Rodriguez
Summary: The study utilized LC-MS/MS combined with online solid-phase extraction (SPE) strategy to detect potential pesticide residues in wine. The method showed high accuracy and provided good recovery rates for 90% of compounds under various parameter conditions.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2021)
Article
Food Science & Technology
Tamara Rodriguez-Cabo, Angeles Morono, Fabiola Arevalo, Jorge Correa, Juan Pablo Lamas, Araceli E. Rossignoli, Juan Blanco
Summary: Samples from Cantabria and the Basque Country in late autumn of 2018 and 2019 were found to be positive for paralytic shellfish poisoning (PSP) toxins using official monitoring systems. Analysis using different methods showed a good correlation and confirmed the presence of various PSP toxins, suggesting a likely causative agent from the genus Alexandrium.
Article
Biochemical Research Methods
M. Cobo-Golpe, M. Ramil, R. Cela, I. Rodriguez
Summary: This article presents a fast and sensitive chiral analysis method for determining eight pharmaceuticals in wastewater and sludge samples. The compounds are separated using supercritical fluid chromatography combined with time-of-flight mass spectrometry detection. Methanol and ammonia are used as modifiers in the mobile phase. The method allows for the analysis of eight pairs of enantiomers in 15.5 minutes and has been successfully applied to the analysis of wastewater and sludge samples.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biochemical Research Methods
M. Cobo Golpe, M. Ramil, I. Rodriguez
Summary: The consumption of electronic cigarettes is increasing, especially among youths. An analysis of e-liquids from different suppliers with different flavors and additives was conducted to understand its potential impact on health. Over 250 chemicals were identified, including respiratory pro-inflammatory compounds, acetals of propylene glycol and glycerin with aldehydes, nicotine-related and non-related alkaloids, and psychoactive cannabinoids. The concentration ratios of propylene glycol acetals to parent aldehydes ranged from 2% to over 80%, and the ratios of delta-9-tetrahydrocannabinol to cannabidiol in e-liquids ranged from 0.02% to 0.3%.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)