Article
Multidisciplinary Sciences
Lyndsay L. Leach, Nicholas J. Hanovice, Stephanie M. George, Ana E. Gabriel, Jeffrey M. Gross
Summary: Research has revealed that zebrafish RPE has strong intrinsic regenerative potential, with elements of immune response identified as critical mediators in the regeneration process. Macrophage/microglia cells are responsive to RPE damage and essential for the timely progression of regenerative response.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemical Research Methods
Deng Pan, Junichi Sayanagi, Jesus A. Acevedo-Cintron, Lauren Schellhardt, Alison K. Snyder-Warwick, Susan E. Mackinnon, Matthew D. Wood
Summary: This study introduced a new method of embedding liposomes within fibrin gels to target macrophages at specific spatial locations in nerve regeneration. The clodronate liposomes showed specific toxicity to macrophages in vitro and effectively depleted macrophages in vivo without affecting other cell types. The delivery system allowed for sustained release of liposomes and demonstrated promising results for nerve regeneration in mice.
JOURNAL OF NEUROSCIENCE METHODS
(2021)
Review
Immunology
Philippe Saas, Mathieu Vetter, Melissa Maraux, Francis Bonnefoy, Sylvain Perruche
Summary: Resolution therapy is a new therapeutic approach for chronic inflammatory diseases that aims to accelerate the resolution phase of inflammation. Macrophages play a critical role in this process by undergoing anti-inflammatory reprogramming and releasing pro-resolving factors. The secretome of efferocytic macrophages, known as SuperMApo, is a drug candidate in resolution therapy.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Endocrinology & Metabolism
Hoyee Wan, Shakira Brathwaite, Jinglu Ai, Kullervo Hynynen, R. Loch Macdonald
Summary: After SAH, erythrocytes are distributed throughout the subarachnoid space and extend into the perivascular spaces of parenchymal arterioles. Meningeal and perivascular macrophages are involved in erythrocyte uptake and play an important role in the outcome after SAH.
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
(2021)
Article
Medicine, Research & Experimental
Candice Bohaud, Jholy De La Cruz, Claudia Terraza, Audrey Barthelaix, Beryl Laplace-Builhe, Christian Jorgensen, Yoan Arribat, Farida Djouad
Summary: This study using zebrafish model and caudal fin resection investigated the crucial role of lactate in macrophage response and fin regeneration. The findings suggest that lactate plays a key role in activating and modulating macrophage phenotype, promoting inflammatory response and tissue regeneration.
Article
Developmental Biology
Aryan Kaveh, Finnius A. Bruton, Magdalena E. M. Oremek, Carl S. Tucker, Jonathan M. Taylor, John J. Mullins, Adriano G. Rossi, Martin A. Denvir
Summary: Sustained neutrophilic inflammation is harmful for cardiac repair after myocardial infarction. CDK9 inhibitor compounds can resolve neutrophil infiltration and promote cardiomyocyte regeneration and cardiac repair.
Article
Food Science & Technology
Shisan Xu, Huan Zhang, Cun-Zhao Li, Ping-Shan Lai, Guijiang Wang, Yu Suen Chan, Shuk Han Cheng, Xueping Chen
Summary: CBD extract accelerates fin regeneration and reduces apoptosis after amputation by modulating the inflammatory response and the IL-1 beta/Caspase 3/PARP signal pathway.
JOURNAL OF FUNCTIONAL FOODS
(2021)
Review
Cell Biology
Maria Iribarne
Summary: The regenerative ability varies greatly among different species, with lower vertebrates like zebrafish possessing outstanding regenerative capacity while mammals can only efficiently restore a few tissues. Evidence suggests that inflammation plays a crucial role in regulating stem cell activity during the process of regeneration.
NEURAL REGENERATION RESEARCH
(2021)
Article
Cell Biology
Mengmeng Jiang, Yanyu Xiao, E. Weigao, Lifeng Ma, Jingjing Wang, Haide Chen, Ce Gao, Yuan Liao, Qile Guo, Jinrong Peng, Xiaoping Han, Guoji Guo
Summary: Utilizing Microwell-seq technology, researchers analyzed over 250,000 single cells of zebrafish, revealing the single-cell compositions of 18 tissue types, including both embryo and adult stages. Through studying caudal fin regeneration, unique characteristics of blastema population and key genetic regulations in zebrafish tissue repair were identified. This single-cell dataset demonstrates the utility of zebrafish cell landscape resources in various fields of biological research.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemical Research Methods
Weijian Gao, Yiqing Zhang, Yuanhan Zhang, Zishan Yuan, Keer Chen, Weilin Xie, Dan Li, Jian Zhang, Lan Zhang
Summary: The study focuses on monitoring the assessment process of a zebrafish inflammation-type skull defects model using spectral-domain optical coherence tomography (SD-OCT), and evaluating the efficacy of etidronate disodium in bone regeneration. The results show that etidronate disodium can reduce inflammatory bone resorption and promote skull defect repair.
JOURNAL OF BIOPHOTONICS
(2023)
Article
Biochemistry & Molecular Biology
Silvia Kuntschar, Giulia Cardamone, Kevin Klann, Rebekka Bauer, Sofie Patrizia Meyer, Rebecca Raue, Peter Rappl, Christian Munch, Bernhard Brune, Tobias Schmid
Summary: This study investigates the translational changes in inflammatory macrophages during the resolution phase, particularly focusing on the role of matrix metallopeptidase 12 (MMP12). The findings suggest that translational control of MMP12 plays an important role in altering the migratory properties of macrophages during the inflammatory response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Yannan Cheng, Hui Chen, Ping Duan, Hao Zhang, Yongle Yu, Jiadong Yu, Zirui Yu, Lin Zheng, Xin Ye, Zhenyu Pan
Summary: M1 macrophages play a crucial role in the pathogenesis of GA-ONFH by promoting inflammation and cellular apoptosis, exacerbating disease progression. Clearance of M1 macrophages can attenuate the development of GA-ONFH, presenting a promising therapeutic strategy.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Iris Ribitsch, Andrea Bileck, Monika Egerbacher, Simone Gabner, Rupert L. Mayer, Lukas Janker, Christopher Gerner, Florien Jenner
Summary: Comparing the response of fetal and adult ovine articular cartilage to injury showed that adult joints exhibited significant cellular changes, while fetal cartilage displayed regeneration capability. Proteomic analysis revealed differential regulation of inflammatory mediators and growth factors between adult and fetal joints, with adult cartilage showing upregulation of neutrophil-related proteins and acute phase proteins following injury, while fetal cartilage expressed higher levels of immunomodulating proteins and growth factors. The comparison of secretome from fetal ovine MSCs with injured fetal cartilage identified potential therapeutic factors for regenerative medicine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Jishou Zhang, Yao Xu, Cheng Wei, Zheng Yin, Wei Pan, Mengmeng Zhao, Wen Ding, Shuwan Xu, Jianfang Liu, Junping Yu, Jing Ye, Di Ye, Juan-Juan Qin, Jun Wan, Menglong Wang
Summary: This study found that Neo1 deficiency aggravates inflammation and left ventricular remodeling after myocardial infarction by modulating macrophage phenotypes and functions via the JAK1-STAT1 signaling pathway. These findings offer new perspectives for therapeutic targets in myocardial infarction treatment.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Review
Immunology
Candice Bohaud, Matt D. D. Johansen, Christian Jorgensen, Natacha Ipseiz, Laurent Kremer, Farida Djouad
Summary: The future of regenerative medicine depends on understanding the mechanistic processes behind tissue regeneration, which has led to the use of zebrafish as a model organism due to its high regenerative capabilities. Recent studies have highlighted the important role of distinct macrophage subpopulations in tissue regeneration, contributing to early inflammation and promoting tissue repair and regeneration in later stages.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Zoology
Nicolas Cumplido, Miguel L. Allende, Gloria Arratia
FRONTIERS IN ZOOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Javiera F. de la Paz, Consuelo Anguita-Salinas, Cesar Diaz-Celis, Francisco P. Chavez, Miguel L. Allende
Biographical-Item
Developmental Biology
Fernando Casares, Miguel Allende, Jose Felix de Celis, Acaimo Gonzalez-Reyes, Juan Ramon Martinez-Morales
Review
Biochemistry & Molecular Biology
David Gonzalez, Miguel L. Allende
Summary: Wallerian degeneration is a genetically conserved mechanism where peripheral nervous system axons have a higher capacity to regenerate. The use of zebrafish larvae and time-lapse microscopy allows for monitoring of the regenerative process of peripheral nerves.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Neurosciences
Susana Paredes-Zuniga, Fernando Ormeno, Miguel L. Allende
Summary: Triadimefon (TDF) is a fungicide used in agricultural crops, which affects the levels of dopamine and serotonin in the brain of vertebrates. This study shows that TDF induces both preference and aversion in zebrafish, depending on the dosage used. The lower dose leads to aversion while the higher dose leads to preference, indicating a complex relationship between TDF and behavioral responses in zebrafish.
NEUROTOXICOLOGY AND TERATOLOGY
(2021)
Article
Cell Biology
Oscar A. Pena, Alexandra Lubin, Jasmine Rowell, Yvette Hoade, Noreen Khokhar, Hanna Lemmik, Christopher Mahony, Phoebe Dace, Chianna Umamahesan, Elspeth M. Payne
Summary: Germline loss or mutation of one copy of the transcription factor GATA2 in humans results in clinical phenotypes affecting hematopoietic, lymphatic, and vascular systems. In this research, zebrafish with two copies of the Gata2 gene were used to investigate the effects of these genes on hematopoiesis during development, revealing unique roles at different stages and a potential redundancy between the two genes. The study also showed defects in the myeloid compartment in adult zebrafish with combined heterozygosity loss, similar to GATA2 loss in humans, adding to our understanding of GATA2 deficiency and its developmental effects.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Hematology
Oscar A. Pena, Alexandra Lubin, Catherine Hockings, Jasmine Rowell, Youngrock Jung, Yvette Hoade, Phoebe Dace, Leonardo E. Valdivia, Karin Tuschl, Charlotta Boiers, Maria C. Virgilio, Simon Richardson, Elspeth M. Payne
Summary: The researchers developed a zebrafish model of MDS and identified imiquimod as a compound that can alleviate anemia by promoting hematopoietic stem and progenitor cell expansion. They also found that TLR7 ligation might play a key role in anemia treatment.
Article
Cell Biology
Oscar E. Diaz, Chiara Sorini, Rodrigo A. Morales, Xinxin Luo, Annika Frede, Annette M. Krais, Myra N. Chavez, Emma Wincent, Srustidhar Das, Eduardo J. Villablanca
Summary: The study revealed that PFOS exacerbates inflammation-induced intestinal damage, disrupts T-cell homeostasis, and provides a novel in vivo toolbox for screening pollutants affecting intestinal homeostasis.
DISEASE MODELS & MECHANISMS
(2021)
Article
Multidisciplinary Sciences
Sara M. Parigi, Ludvig Larsson, Srustidhar Das, Ricardo O. Ramirez Flores, Annika Frede, Kumar P. Tripathi, Oscar E. Diaz, Katja Selin, Rodrigo A. Morales, Xinxin Luo, Gustavo Monasterio, Camilla Engblom, Nicola Gagliani, Julio Saez-Rodriguez, Joakim Lundeberg, Eduardo J. Villablanca
Summary: In this study, the authors used spatial transcriptomics to investigate the transcriptomic regionalization of the colon during steady state and mucosal healing. They found that the colon exhibits molecular regionalization in steady state conditions, which undergoes significant changes during mucosal healing. They identified spatially-organized transcriptional programs associated with mucosal healing and regions with dominant wired pathways. They also discovered a correlation between decreased p53 activation and increased presence of proliferating epithelial stem cells. Additionally, they mapped transcriptomics modules associated with human diseases, indicating the translational potential of their dataset.
NATURE COMMUNICATIONS
(2022)
Article
Immunology
Hassan Melhem, Berna Kaya, Tanay Kaymak, Philipp Wuggenig, Emilio Flint, Julien Roux, Koen C. Oost, Claudia Cavelti-Weder, Maria L. Balmer, Jean-Claude Walser, Rodrigo A. Morales, Christian U. Riedel, Prisca Liberali, Eduardo J. Villablanca, Jan Hendrik Niess
Summary: This study reveals the critical role of Gpr35 in goblet cell function and its regulation of goblet cell numbers and microbiota balance through maintaining normal pyroptosis levels. It is essential for maintaining intestinal health and the symbiotic relationship between host and microbiota.
MUCOSAL IMMUNOLOGY
(2022)
Article
Immunology
Rodrigo A. Morales, Soraya Rabahi, Oscar E. Diaz, Yazan Salloum, Bianca C. Kern, Mikaela Westling, Xinxin Luo, Sara M. Parigi, Gustavo Monasterio, Srustidhar Das, Pedro P. Hernandez, Eduardo J. Villablanca
Summary: This study reveals the previously underappreciated IL-10-Notch axis that regulates goblet cell homeostasis in the developing zebrafish intestine. It also suggests conservation across species and may help explain the disease severity of IL-10 deficiency in mammalian intestines.
MUCOSAL IMMUNOLOGY
(2022)
Article
Immunology
Annika Frede, Paulo Czarnewski, Gustavo Monasterio, Kumar P. Tripathi, David A. Bejarano, Ricardo O. Ramirez Flores, Chiara Sorini, Ludvig Larsson, Xinxin Luo, Laura Geerlings, Claudio Novella-Rausell, Chiara Zagami, Raoul Kuiper, Rodrigo A. Morales, Francisca Castillo, Matthew Hunt, Livia Lacerda Mariano, Yue O. O. Hu, Camilla Engblom, Ana-Maria Lennon-Dumenil, Romy Mittenzwei, Astrid M. Westendorf, Nadine Hoevelmeyer, Joakim Lundeberg, Julio Saez-Rodriguez, Andreas Schlitzer, Srustidhar Das, Eduardo J. Villablanca
Summary: In the process of intestinal damage and regeneration, B cells play a dominant role and show expansion of an IFN-induced B cell subset during mucosal healing. Depletion of B cells accelerates recovery, reduces epithelial ulceration, and enhances gene expression programs associated with tissue remodeling. The expansion of B cells impairs interactions between epithelial and stromal cells, hindering mucosal healing.
Article
Medicine, General & Internal
Sravani Devarakonda, Annika Thorsell, Per Hedenstrom, Azar Rezapour, Lisen Heden, Sanghita Banerjee, Malin E. V. Johansson, George Birchenough, Amelie Toft Moren, Karin Gustavsson, Viktor Skokic, Victor L. Pettersson, Fei Sjoberg, Marie Kalm, Mohammad Al Masri, Michaela Ekh, Henrik Fagman, Mats Wolving, Rosie Perkins, Rodrigo A. Morales, Francisca Castillo, EduardoJ. Villablanca, Ulf Yrlid, Karin Bergmark, Gunnar Steineck, Cecilia Bull
Summary: Radiotherapy is effective in cancer treatment but can also harm non-cancerous tissue. This study examined the presence of chronic inflammation in mucosal biopsies from pelvic cancer survivors who received radiotherapy. The findings suggest the presence of a chronic low-grade inflammation with neutrophil activity, supporting the exploration of new strategies to alleviate chronic bowel symptoms in these survivors.
Article
Biochemistry & Molecular Biology
Giuseppina Covello, Fernando J. Rossello, Michele Filosi, Felipe Gajardo, Anne-Laure Duchemin, Beatrice F. Tremonti, Michael Eichenlaub, Jose M. Polo, David Powell, John Ngai, Miguel L. Allende, Enrico Domenici, Mirana Ramialison, Lucia Poggi