Rapid hemostasis and excellent antibacterial cerium-containing mesoporous bioactive glass/chitosan composite sponge for hemostatic material

Rapid and effective hemostasis is key to controlling bleeding and reducing mortality. Here, a composite hemostatic sponge (Ce-MBG/CHT) of cerium-containing mesoporous bioactive glass (Ce-MBG) and chitosan (CHT) was prepared by a freeze-drying technique and compared with the commercially available gelatin sponge (GS), to further evaluate the hemostatic performance of composite sponge materials. The results indicate that the pore structure, porosity, and water absorption of the sponge improved following the addition of Ce-MBG. Whole blood coagulation studies suggested that Ce-MBG/CHT has superior hemostatic properties to GS and validated in vitro thrombosis, platelet adhesion and blood compatibility. In vitro coagulation studies showed that factor XII was activated by the addition of Ce-MBG, inducing the intrinsic coagulation pathway. Furthermore, we evaluated the cytocompatibility of samples after contact with L929 cells for 24, 48, and 72 h via the cytotoxicity test. Compared with GS, 4Ce-MBG/CHT was more efficient against E. coli and S. aureus. All these results indicate that Ce-MBG/CHT sponges are likely useful for rapid hemostasis. (c) 2021 Elsevier Ltd. All rights reserved.

Automated summary

In this study, a composite hemostatic sponge composed of cerium-containing mesoporous bioactive glass and chitosan was prepared and compared with a commercially available gelatin sponge. The results showed that the composite sponge had superior hemostatic performance and validated its thrombosis, platelet adhesion, and blood compatibility. Additionally, the composite sponge exhibited strong antibacterial effects against common bacteria.