Article
Chemistry, Multidisciplinary
Crystal S. Shin, Fernando J. Cabrera, Richard Lee, John Kim, Remya Ammassam Veettil, Mahira Zaheer, Aparna Adumbumkulath, Kirti Mhatre, Pulickel M. Ajayan, Steven A. Curley, Bradford G. Scott, Ghanashyam Acharya
Summary: The development of an inflammation modulating biomaterial scaffold for soft tissue repair has shown promising results in inhibiting visceral adhesions formation and minimizing postsurgical complications. In vivo evaluation in a rat ventral hernia model confirmed that the bioscaffold acts as an inflammation trap, capturing proinflammatory cytokines and modulating local inflammation effectively without the need for exogenous anti-inflammatory agents.
ADVANCED MATERIALS
(2021)
Article
Chemistry, Applied
Isabella Nacu, Maria Bercea, Loredana Elena Nita, Catalina Anisoara Peptu, Maria Butnaru, Liliana Verestiuc
Summary: The application of 3D bioprinting in the medical field requires optimized bioinks/biomaterials and scaffolds with morphology and structure similar to human tissues. Polymeric hydrogels have been used in the elaboration of bioinks to support cellular biology. This study presents the preparation of versatile bioadhesive hydrogels based on methacrylated gelatin, crosslinked with poly(ethylene glycol) diacrylate, and 3D bioprinted as bioadhesive scaffolds for skin tissue engineering. The scaffolds show good cytocompatibility and increased bioadhesion characteristics when poly(ethylene glycol) diacrylate is added as a crosslinker.
REACTIVE & FUNCTIONAL POLYMERS
(2023)
Article
Pharmacology & Pharmacy
Pablo Edmundo Antezana, Sofia Municoy, Maria Ines Alvarez-Echazu, Pablo Luis Santo-Orihuela, Paolo Nicolas Catalano, Taleb H. Al-Tel, Firoz Babu Kadumudi, Alireza Dolatshahi-Pirouz, Gorka Orive, Martin Federico Desimone
Summary: This review discusses the latest developments in skin tissue engineering using 3D bioprinting as a new tool. It provides an overview of current bioprinting methods and summarizes bioink formulations, parameters, and properties. Representative examples and advances in the field, as well as limitations and future needs, are also discussed.
Article
Engineering, Biomedical
Anne Behre, Joshua W. Tashman, Caner Dikyol, Daniel J. Shiwarski, Raphael J. Crum, Scott A. Johnson, Remya Kommeri, George S. Hussey, Stephen F. Badylak, Adam W. Feinberg
Summary: This study develops a process using 3D bioprinting and computed tomography imaging to fabricate large volume, patient-specific dECM patches for soft tissue wounds. The analysis shows that these patches are dimensionally accurate and adapt well to complex wound surfaces. Additionally, the study demonstrates the ability to create clinically relevant dECM scaffolds with precise control over fiber alignment and micro-architecture.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
R. Olmos-Juste, G. Larranaga-Jaurrieta, I. Larraza, S. Ramos-Diez, S. Camarero-Espinosa, N. Gabilondo, A. Eceiza
Summary: In this study, functional alginate and waterborne polyurethane scaffolds were developed for articular cartilage regeneration using 3D bioprinting technology. It was found that scaffolds with a higher alginate content showed better 3D printing performance, structural integrity, and cell viability. After 28 days of experiments, these scaffolds were capable of maintaining cell numbers and synthesizing glycosaminoglycans, indicating their potential application for in vitro regeneration of articular cartilage tissue.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Engineering, Biomedical
Chengxiong Lin, Yaocheng Wang, Zhengyu Huang, Tingting Wu, Weikang Xu, Wenming Wu, Zhibiao Xu
Summary: This paper investigates the filament structure of 3D bioprinted biodegradable bone repair scaffolds and discusses the characteristics and performance of different structures. The novel scaffolds demonstrate excellent performance in terms of material composition, ink configuration, printing parameters, and additional functions such as chondrogenesis, angiogenesis, anti-bacteria, and anti-tumor effects.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2021)
Review
Pharmacology & Pharmacy
Maria Lazaridou, Dimitrios N. Bikiaris, Dimitrios A. Lamprou
Summary: Bioprinting is a promising technology for developing functional tissue constructs and controlled drug delivery systems. Chitosan, a natural polysaccharide, is an attractive bioink for tissue regeneration and restoration.
Review
Materials Science, Multidisciplinary
Na Liu, Xiaopei Zhang, Qingxia Guo, Tong Wu, Yuanfei Wang
Summary: Three-dimensional (3D) printing technology has revolutionized the manufacturing process and has great potential for tissue repair and regeneration. 3D bioprinting can create complex and biomimetic 3D structures for tissue engineering, and it has significant effects on nerve, skin, blood vessels, bones, and cartilage injury and regeneration. However, there are still clinical challenges and future opportunities that need to be addressed for the further development of 3D bioprinting.
FRONTIERS IN MATERIALS
(2022)
Review
Biotechnology & Applied Microbiology
Zahra Yazdanpanah, James D. Johnston, David M. L. Cooper, Xiongbiao Chen
Summary: Treating large bone defects is challenging and bone tissue engineering using 3D bioprinted scaffolds has emerged as a promising approach. This review highlights the latest developments in the use of bioprinted scaffolds for critical-sized defect treatment, including the biomaterials, cells, and growth factors used in bioink formulation and bioprinting techniques. The review also discusses current limitations and emerging approaches in this field.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Markel Lafuente-Merchan, Sandra Ruiz-Alonso, Fatima Garcia-Villen, Alaitz Zabala, Ana M. Ochoa de Retana, Idoia Gallego, Laura Saenz-Del-Burgo, Jose Luis Pedraz
Summary: Bone tissue damage is a common problem associated with various diseases and trauma. The current treatments have limitations, making tissue engineering through 3D bioprinting a promising option for bone regenerative medicine. In this study, the addition of hydroxyapatite (HAP) and graphene oxide (GO) to nanocellulose-alginate (NC-Alg) bioink improves its viscosity and enhances scaffold mechanics, cell viability, and functionality. However, GO is incompatible with the ink after autoclave sterilization. Overall, the results highlight the importance of using inorganic components in bioink for bone tissue engineering.
MACROMOLECULAR BIOSCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Nurulhuda Mohd, Masfueh Razali, Mh Busra Fauzi, Noor Hayaty Abu Kasim
Summary: Three-dimensional (3D) bioprinting combines 3D printing and tissue engineering, offering potential for repairing and replacing damaged tissues in dental treatments. Recent research has focused on different techniques and strategies for 3D bioprinting, particularly in using cell-laden bioinks, to overcome challenges in constructing biomimetic scaffolds. This review examines the in vitro applications of 3D-bioprinted scaffolds on cell viability, functionality, differentiation, and marker expression, as well as the in vivo evaluations on animal models for various dental tissue regenerations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Instruments & Instrumentation
Katie Glover, Essyrose Mathew, Giulia Pitzanti, Erin Magee, Dimitrios A. Lamprou
Summary: The treatment strategy for diabetic foot ulcer (DFU) is complex, requires multiple therapeutic approaches, and often results in amputation. Bioprinting technologies show innovative potential in fabricating antibiotic scaffolds for DFU treatment.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2023)
Article
Polymer Science
Linnea Warburton, Boris Rubinsky
Summary: Temperature-Controlled-Cryoprinting (TCC) is a new 3D bioprinting technology that allows for the fabrication and cryopreservation of complex and large cell-laden scaffolds. It demonstrated high cell viability and no size limitations in fabricating and cryopreserving 3D alginate-based scaffolds using TCC. The findings suggest that TCC has significant potential for advancing 3D cell culture and tissue engineering.
Article
Engineering, Biomedical
Karli A. Gold, Biswajit Saha, Navaneeth Krishna Rajeeva Pandian, Brandon K. Walther, Jorge A. Palma, Javier Jo, John P. Cooke, Abhishek Jain, Akhilesh K. Gaharwar
Summary: The introduction of a new class of nanoengineered hydrogel-based cell-laden bioinks in 3D bioprinting shows promising potential for replicating the microenvironment of human vasculature, with high printability and cell protection. The ability to print anatomically accurate, multicellular blood vessels provides a valuable tool for modeling vascular function and pathophysiology, demonstrating thromboinflammatory responses similar to in vitro and in vivo models.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Nanoscience & Nanotechnology
Thiago Domingues Stocco, Mayara Cristina Moreira Silva, Marcus Alexandre Finzi Corat, Gabriely Goncalves Lima, Anderson Oliveira Lobo
Summary: This study presents a new biofabrication strategy for developing biomimetic meniscus scaffolds. The use of bioprinted cell-laden hydrogel reinforced by aligned nanofiber sheets improved the structural integrity, shape fidelity, and mechanical properties of the scaffolds. Importantly, the introduction of nanofiber mats did not negatively affect cell viability. This bioengineering approach offers a promising strategy for tissue engineering of the knee meniscus.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2022)
Article
Materials Science, Multidisciplinary
Seyed Shahaboddin Ayati, Mehdi Karevan, Evan Stefanek, Mohammed Bhia, Mohsen Akbari
Summary: This study presents a new method for fabricating nanofibers using centrifugal spinning and solution blowing. The process involves a rotating reservoir and high velocity expanding gas to extrude fibers. The morphology of the fibers depends on various factors.
MACROMOLECULAR MATERIALS AND ENGINEERING
(2022)
Review
Chemistry, Analytical
Mostafa Azimzadeh, Patricia Khashayar, Meitham Amereh, Nishat Tasnim, Mina Hoorfar, Mohsen Akbari
Summary: Quantification of oxygen (O-2) is crucial for assessing cell metabolism and viability. Recent advances in microfluidics have made O-2 sensing a crucial feature in organ-on-chip devices. This review provides an overview of on-chip O-2 sensors integrated with organ-on-chip devices and evaluates their advantages and disadvantages.
Article
Chemistry, Multidisciplinary
Amir Seyfoori, Seyyed Ali Seyyed Ebrahimi, Mohamadmahdi Samandari, Ehsan Samiei, Evan Stefanek, Cathie Garnis, Mohsen Akbari
Summary: Capturing rare disease-associated biomarkers from body fluids can provide early-stage diagnosis of different cancers. This paper introduces a novel method for making soft micromagnet patterns with optimized geometry and magnetic material, integrated into a bilayer microfluidic chip to enhance the capture efficiency of cancer cells. The proposed design enables high capture efficiency and purity of target cells, as well as real-time monitoring of their behavior. This strategy offers a simple, low-cost, and robust opportunity for early-stage diagnosis and monitoring of cancer-associated biomarkers.
Review
Engineering, Biomedical
Mahsa Janmohammadi, Zahra Nazemi, Amin Orash Mahmoud Salehi, Amir Seyfoori, Johnson John, Mohammad Sadegh Nourbakhsh, Mohsen Akbari
Summary: Cellulose-based scaffolds have great potential in bone tissue engineering due to their non-toxicity, biocompatibility, renewable nature, and low cost. They can deliver growth factors and antibiotics directly to the site of impaired bone tissue to promote tissue repair. This review focuses on the various types of cellulose-based composite scaffolds used for bone drug delivery systems and bone regeneration, highlighting their physicochemical, mechanical, and biological properties.
BIOACTIVE MATERIALS
(2023)
Article
Engineering, Biomedical
Dana Hellmold, Pietro Arnaldi, Michael Synowitz, Janka Held-Feindt, Mohsen Akbari
Summary: Current treatment strategies for GBM have limited effectiveness, so there is a need for more effective treatments. A localized drug delivery system called AT101-GlioMesh, which releases the drug AT101 at the tumor site, has shown promising results in inhibiting tumor recurrence and inducing cytotoxic effects on GBM cell lines.
BIOMEDICAL MATERIALS
(2023)
Article
Engineering, Biomedical
Bahram Mirani, Zhina Hadisi, Erik Pagan, Seyed Mohammad Hossein Dabiri, Antonia van Rijt, Lama Almutairi, Iman Noshadi, David G. Armstrong, Mohsen Akbari
Summary: Managing slow-healing wounds and associated complications is challenging. Systematic collection, analysis, and dissemination of correct wound status data are critical for enhancing healing outcomes and reducing complications.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Polymer Science
Mahmood Razzaghi, Amir Seyfoori, Erik Pagan, Esfandyar Askari, Alireza Hassani Najafabadi, Mohsen Akbari
Summary: To address the need for continuous monitoring and treatment adjustments in chronic diseases, a microneedle array technique is proposed to extract interstitial skin fluid (ISF) painlessly and bloodlessly. The molecular composition of ISF resembles blood plasma, making it a good choice for biomarker identification. The integration of a multiplexed sensor in the microneedle array enables rapid biomarker detection, allowing for practical and self-administrable monitoring and management of metabolic diseases.
Article
Chemistry, Analytical
Mahmood Razzaghi, Mohsen Akbari
Summary: Microneedle arrays (MNAs) are emerging devices used for drug delivery and diagnostics through the skin. 3D printing-based fabrication methods offer advantages such as faster and more precise fabrication, but the penetration capability into the skin needs improvement.
Review
Engineering, Biomedical
Esfandyar Askari, Mahdieh Shokrollahi Barough, Mehdi Rahmanian, Nazanin Mojtabavi, Ramin Sarrami Forooshani, Amir Seyfoori, Mohsen Akbari
Summary: This article summarizes the applications of micro/nanostructured hydrogels in cancer immunotherapy, focusing on their local and systemic effects and potential clinical translation. Hydrogels as carriers can stimulate immune cells by releasing tumor lysate, drugs, or nanovaccines, activating systemic antitumor immune responses and inhibiting tumor metastasis and recurrence.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Pharmacology & Pharmacy
Brent Godau, Sadaf Samimi, Amir Seyfoori, Ehsan Samiei, Tahereh Khani, Parvaneh Naserzadeh, Alireza Hassani Najafabadi, Emal Lesha, Keivan Majidzadeh-A, Behnaz Ashtari, Gabriel Charest, Christophe Morin, David Fortin, Mohsen Akbari
Summary: Injectable shear-thinning hydrogel (STH) acts as a non-invasive local delivery vehicle for slow-releasing anticancer drugs, reducing systemic toxicity and improving treatment effectiveness. Experimental results show that STH injected into tumor tissue significantly increases overall survival in animal models of breast cancer and glioblastoma, demonstrating potential for localized treatment.
Review
Polymer Science
Amirhossein Sadeghi, Shadi Poureskandar, Esfandyar Askari, Mohsen Akbari
Summary: Polymeric nanomaterials, nanogels, and solid nanoparticles can be made using single or double emulsion methods. These materials show promise for biomedical applications due to their biocompatibility, biodegradability, and ability to interact with body fluids and cells. However, the understanding of the biological behavior of polymeric nanoparticles is incomplete.
Article
Engineering, Multidisciplinary
Meitham Amereh, Amir Seyfoori, Briana Dallinger, Mostafa Azimzadeh, Evan Stefanek, Mohsen Akbari
Summary: This study investigated the effect of matrix stiffness on the invasion pattern of human glioblastoma tumoroids. A tumor-on-a-chip platform was used to culture the tumoroids and evaluate the effect of stiffness on tumor progression. Results showed that tumoroids exhibited two distinct invasion patterns, ring-type and finger-type, in response to collagenase concentration. Higher concentrations of collagenase resulted in greater invasion lengths, highlighting the dependence of tumor behavior on the stiffness of the surrounding matrix. The use of a mathematical model provided insights into the mechanisms involved in GBM invasion in an inhomogeneous environment.
Review
Materials Science, Biomaterials
Ali Mousavi, Maedeh Rahimnejad, Mostafa Azimzadeh, Mohsen Akbari, Houman Savoji
Summary: Electronic skins have revolutionized the lives of patients with skin and muscle dysfunction. This review highlights the recent advancements in sensory function of electronic skins and categorizes them based on tactile sensors, temperature sensors, and multi-sensors. The role of material selection and structure in sensory function is also discussed.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
