标题
Increasing the Targeting Scope of CRISPR Base Editing System Beyond NGG
作者
关键词
-
出版物
CRISPR Journal
Volume 5, Issue 2, Pages 187-202
出版商
Mary Ann Liebert Inc
发表日期
2022-03-03
DOI
10.1089/crispr.2021.0109
参考文献
相关参考文献
注意:仅列出部分参考文献,下载原文获取全部文献信息。- Efficient and high-fidelity base editor with expanded PAM compatibility for cytidine dinucleotide
- (2021) Zhiquan Liu et al. Science China-Life Sciences
- In vivo cytidine base editing of hepatocytes without detectable off-target mutations in RNA and DNA
- (2021) Lukas Villiger et al. Nature Biomedical Engineering
- Programmable C:G to G:C genome editing with CRISPR-Cas9-directed base excision repair proteins
- (2021) Liwei Chen et al. Nature Communications
- CRISPR-Cas9 cytidine and adenosine base editing of splice-sites mediates highly-efficient disruption of proteins in primary and immortalized cells
- (2021) Mitchell G. Kluesner et al. Nature Communications
- Rationally Designed Base Editors for Precise Editing of the Sickle Cell Disease Mutation
- (2021) S. Haihua Chu et al. CRISPR Journal
- Efficient C•G-to-G•C base editors developed using CRISPRi screens, target-library analysis, and machine learning
- (2021) Luke W. Koblan et al. NATURE BIOTECHNOLOGY
- Enhanced prime editing systems by manipulating cellular determinants of editing outcomes
- (2021) Peter J. Chen et al. CELL
- Targeted gene disruption by CRISPR/xCas9 system in Drosophila melanogaster
- (2020) Xu‐yang Ni et al. ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
- CRISPR-Based Adenine Editors Correct Nonsense Mutations in a Cystic Fibrosis Organoid Biobank
- (2020) Maarten H. Geurts et al. Cell Stem Cell
- Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9
- (2020) Daniel Agudelo et al. GENOME RESEARCH
- Continuous evolution of SpCas9 variants compatible with non-G PAMs
- (2020) Shannon M. Miller et al. NATURE BIOTECHNOLOGY
- Programmable base editing of mutated TERT promoter inhibits brain tumour growth
- (2020) Xinjian Li et al. NATURE CELL BIOLOGY
- Expanding the genome-targeting scope and the site selectivity of high-precision base editors
- (2020) Junjie Tan et al. Nature Communications
- High-Throughput Screens of PAM-Flexible Cas9 Variants for Gene Knockout and Transcriptional Modulation
- (2020) Mateusz Legut et al. Cell Reports
- High-throughput analysis of the activities of xCas9, SpCas9-NG and SpCas9 at matched and mismatched target sequences in human cells
- (2020) Hui Kwon Kim et al. Nature Biomedical Engineering
- Base editing-mediated splicing correction therapy for spinal muscular atrophy
- (2020) Xiang Lin et al. CELL RESEARCH
- Quantification of the affinities of CRISPR–Cas9 nucleases for cognate protospacer adjacent motif (PAM) sequences
- (2020) Vladimir Mekler et al. JOURNAL OF BIOLOGICAL CHEMISTRY
- Directed evolution of adenine base editors with increased activity and therapeutic application
- (2020) Nicole M. Gaudelli et al. NATURE BIOTECHNOLOGY
- Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity
- (2020) Michelle F. Richter et al. NATURE BIOTECHNOLOGY
- An engineered ScCas9 with broad PAM range and high specificity and activity
- (2020) Pranam Chatterjee et al. NATURE BIOTECHNOLOGY
- Increasing the efficiency and targeting range of cytidine base editors through fusion of a single-stranded DNA-binding protein domain
- (2020) Xiaohui Zhang et al. NATURE CELL BIOLOGY
- Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants
- (2020) Russell T. Walton et al. SCIENCE
- A Cas9 with PAM recognition for adenine dinucleotides
- (2020) Pranam Chatterjee et al. Nature Communications
- Efficient generation of mouse models with the prime editing system
- (2020) Yao Liu et al. Cell Discovery
- CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells
- (2020) Ibrahim C. Kurt et al. NATURE BIOTECHNOLOGY
- Glycosylase base editors enable C-to-A and C-to-G base changes
- (2020) Dongdong Zhao et al. NATURE BIOTECHNOLOGY
- A positive, growth-based PAM screen identifies noncanonical motifs recognized by the S. pyogenes Cas9
- (2020) D. Collias et al. Science Advances
- Genome scale analysis of pathogenic variants targetable for single base editing
- (2020) Alexander V. Lavrov et al. BMC Medical Genomics
- Expanding base editing scope to near-PAMless with engineered CRISPR/Cas9 variants in plants
- (2020) Chengwei Zhang et al. Molecular Plant
- Phage-assisted continuous and non-continuous evolution
- (2020) Shannon M. Miller et al. Nature Protocols
- Cytosine Base Editor (hA3A-BE3-NG)-Mediated Multiple Gene Editing for Pyramid Breeding in Pigs
- (2020) Yu Wang et al. Frontiers in Genetics
- CRISPR-Cas9 Circular Permutants as Programmable Scaffolds for Genome Modification
- (2019) Benjamin L. Oakes et al. CELL
- Structural insights into a high fidelity variant of SpCas9
- (2019) Minghui Guo et al. CELL RESEARCH
- Phage-Assisted Evolution of Bacillus methanolicus Methanol Dehydrogenase 2
- (2019) Timothy B. Roth et al. ACS Synthetic Biology
- Engineering of high-precision base editors for site-specific single nucleotide replacement
- (2019) Junjie Tan et al. Nature Communications
- Engineered CRISPR–Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing
- (2019) Benjamin P. Kleinstiver et al. NATURE BIOTECHNOLOGY
- Improving plant genome editing with high-fidelity xCas9 and non-canonical PAM-targeting Cas9-NG
- (2019) Zhaohui Zhong et al. Molecular Plant
- Improved base editor for efficient editing in GC contexts in rabbits with an optimized AID-Cas9 fusion
- (2019) Zhiquan Liu et al. FASEB JOURNAL
- Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors
- (2019) Tony P. Huang et al. NATURE BIOTECHNOLOGY
- Expanded targeting scope and enhanced base editing efficiency in rabbit using optimized xCas9(3.7)
- (2019) Zhiquan Liu et al. CELLULAR AND MOLECULAR LIFE SCIENCES
- Improving Editing Efficiency for the Sequences with NGH PAM Using xCas9-Derived Base Editors
- (2019) Xinyi Liu et al. Molecular Therapy-Nucleic Acids
- Highly efficient base editing with expanded targeting scope using SpCas9‐NG in rabbits
- (2019) Zhiquan Liu et al. FASEB JOURNAL
- Efficient Gene Silencing by Adenine Base Editor-Mediated Start Codon Mutation
- (2019) Xinjie Wang et al. MOLECULAR THERAPY
- Search-and-replace genome editing without double-strand breaks or donor DNA
- (2019) Andrew V. Anzalone et al. NATURE
- Efficient base editing with expanded targeting scope using an engineered Spy-mac Cas9 variant
- (2019) Zhiquan Liu et al. Cell Discovery
- SaCas9 Requires 5′-NNGRRT-3′ PAM for Sufficient Cleavage and Possesses Higher Cleavage Activity than SpCas9 or FnCpf1 in Human Cells
- (2018) Haihua Xie et al. Biotechnology Journal
- BE-PLUS: a new base editing tool with broadened editing window and enhanced fidelity
- (2018) Wen Jiang et al. CELL RESEARCH
- Base editing with a Cpf1–cytidine deaminase fusion
- (2018) Xiaosa Li et al. NATURE BIOTECHNOLOGY
- SaCas9 Requires 5′-NNGRRT-3′ PAM for Sufficient Cleavage and Possesses Higher Cleavage Activity than SpCas9 or FnCpf1 in Human Cells
- (2018) Haihua Xie et al. Biotechnology Journal
- Evolved Cas9 variants with broad PAM compatibility and high DNA specificity
- (2018) Johnny H. Hu et al. NATURE
- Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion
- (2018) Xiao Wang et al. NATURE BIOTECHNOLOGY
- An APOBEC3A-Cas9 base editor with minimized bystander and off-target activities
- (2018) Jason M Gehrke et al. NATURE BIOTECHNOLOGY
- Expanding the base editing scope in rice by using Cas9 variants
- (2018) Kai Hua et al. PLANT BIOTECHNOLOGY JOURNAL
- Engineered CRISPR-Cas9 nuclease with expanded targeting space
- (2018) Hiroshi Nishimasu et al. SCIENCE
- Directed evolution of CRISPR-Cas9 to increase its specificity
- (2018) Jungjoon K. Lee et al. Nature Communications
- Increasing targeting scope of adenosine base editors in mouse and rat embryos through fusion of TadA deaminase with Cas9 variants
- (2018) Lei Yang et al. Protein & Cell
- A Compact, High-Accuracy Cas9 with a Dinucleotide PAM for In Vivo Genome Editing
- (2018) Alireza Edraki et al. MOLECULAR CELL
- Efficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A
- (2018) Yuan Zong et al. NATURE BIOTECHNOLOGY
- Treatment of a metabolic liver disease by in vivo genome base editing in adult mice
- (2018) Lukas Villiger et al. NATURE MEDICINE
- xC as9 expands the scope of genome editing with reduced efficiency in rice
- (2018) Junjie Wang et al. PLANT BIOTECHNOLOGY JOURNAL
- Targeting fidelity of adenine and cytosine base editors in mouse embryos
- (2018) Hye Kyung Lee et al. Nature Communications
- Minimal PAM specificity of a highly similar SpCas9 ortholog
- (2018) Pranam Chatterjee et al. Science Advances
- Structural Basis for the Canonical and Non-canonical PAM Recognition by CRISPR-Cpf1
- (2017) Takashi Yamano et al. MOLECULAR CELL
- CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons
- (2017) Pierre Billon et al. MOLECULAR CELL
- Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage
- (2017) Nicole M. Gaudelli et al. NATURE
- Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions
- (2017) Y Bill Kim et al. NATURE BIOTECHNOLOGY
- CRISPR-STOP: gene silencing through base-editing-induced nonsense mutations
- (2017) Cem Kuscu et al. NATURE METHODS
- Rapid, Selection-Free, High-Efficiency Genome Editing in Protozoan Parasites Using CRISPR-Cas9 Ribonucleoproteins
- (2017) Lia Carolina Soares Medeiros et al. mBio
- In vivo genome editing with a small Cas9 orthologue derived from Campylobacter jejuni
- (2017) Eunji Kim et al. Nature Communications
- Programmable base editing of zebrafish genome using a modified CRISPR-Cas9 system
- (2017) Yihan Zhang et al. Nature Communications
- Highly efficient base editing in human tripronuclear zygotes
- (2017) Changyang Zhou et al. Protein & Cell
- Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity
- (2017) Alexis C. Komor et al. Science Advances
- Crystal Structure of Cpf1 in Complex with Guide RNA and Target DNA
- (2016) Takashi Yamano et al. CELL
- Structural Plasticity of PAM Recognition by Engineered Variants of the RNA-Guided Endonuclease Cas9
- (2016) Carolin Anders et al. MOLECULAR CELL
- Structural Basis for the Altered PAM Specificities of Engineered CRISPR-Cas9
- (2016) Seiichi Hirano et al. MOLECULAR CELL
- Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage
- (2016) Alexis C. Komor et al. NATURE
- High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects
- (2016) Benjamin P. Kleinstiver et al. NATURE
- Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells
- (2016) Benjamin P Kleinstiver et al. NATURE BIOTECHNOLOGY
- Genome-wide analysis reveals specificities of Cpf1 endonucleases in human cells
- (2016) Daesik Kim et al. NATURE BIOTECHNOLOGY
- Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells
- (2016) Gaelen T Hess et al. NATURE METHODS
- Teleseismic S wave microseisms
- (2016) K. Nishida et al. SCIENCE
- Structures of a CRISPR-Cas9 R-loop complex primed for DNA cleavage
- (2016) F. Jiang et al. SCIENCE
- Crystal Structure of Staphylococcus aureus Cas9
- (2015) Hiroshi Nishimasu et al. CELL
- Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System
- (2015) Bernd Zetsche et al. CELL
- Engineered CRISPR-Cas9 nucleases with altered PAM specificities
- (2015) Benjamin P. Kleinstiver et al. NATURE
- In vivo genome editing using Staphylococcus aureus Cas9
- (2015) F. Ann Ran et al. NATURE
- Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition
- (2015) Benjamin P Kleinstiver et al. NATURE BIOTECHNOLOGY
- Dynamics of CRISPR-Cas9 genome interrogation in living cells
- (2015) Spencer C. Knight et al. SCIENCE
- Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA
- (2014) Hiroshi Nishimasu et al. CELL
- Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease
- (2014) Carolin Anders et al. NATURE
- DNA interrogation by the CRISPR RNA-guided endonuclease Cas9
- (2014) Samuel H. Sternberg et al. NATURE
- Direct observation of R-loop formation by single RNA-guided Cas9 and Cascade effector complexes
- (2014) Mark D. Szczelkun et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- Structures of Cas9 Endonucleases Reveal RNA-Mediated Conformational Activation
- (2014) M. Jinek et al. SCIENCE
- Comparison of non-canonical PAMs for CRISPR/Cas9-mediated DNA cleavage in human cells
- (2014) Yilan Zhang et al. Scientific Reports
- RNA-guided editing of bacterial genomes using CRISPR-Cas systems
- (2013) Wenyan Jiang et al. NATURE BIOTECHNOLOGY
- A system for the continuous directed evolution of biomolecules
- (2011) Kevin M. Esvelt et al. NATURE
- The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA
- (2010) Josiane E. Garneau et al. NATURE
- Short motif sequences determine the targets of the prokaryotic CRISPR defence system
- (2009) MICROBIOLOGY-SGM
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