Review
Cardiac & Cardiovascular Systems
Sophia E. Silver, Ryan W. Barrs, Ying Mei
Summary: Cardiovascular disease is a leading cause of global mortality, and alternative cardiac regenerative therapies, such as hPSC-derived cardiomyocytes, show promising therapeutic potential for heart repair. Despite challenges and limitations, solutions are being explored to address these issues towards successful clinical translation and future perspectives on hPSC-CM transplantation.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Cardiac & Cardiovascular Systems
Thomas Eschenhagen, Katrin Ridders, Florian Weinberger
Summary: Heart regeneration is essential for treating heart disease by remuscularizing lost myocardium. While there has been progress in the past decade, there are still many important questions to be answered in order to optimize this approach for regenerative therapy of heart failure.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Emma Claire James, Eva Tomaskovic-Crook, Jeremy Micah Crook
Summary: The regenerative capacity of cardiomyocytes is insufficient for functional recovery, leading to ischaemic heart disease as a major cause of cardiovascular deaths. While human-induced pluripotent stem cells (hiPSCs) show potential for modeling heart disease and replacement therapy, challenges remain with the mixed populations and immature properties of hiPSC-derived cardiomyocytes, which are being addressed by innovative bioengineering technologies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cardiac & Cardiovascular Systems
Xi Lou, Yawen Tang, Lei Ye, Danielle Pretorius, Vladimir G. Fast, Asher M. Kahn-Krell, Jue Zhang, Jianhua Zhang, Aijun Qiao, Gangjian Qin, Timothy Kamp, James A. Thomson, Jianyi Zhang
Summary: The study demonstrates that human cardiac muscle patches containing three types of cardiac cells derived from human pluripotent stem cells significantly improved cardiac function, reduced infarct size, and promoted cardiac maturation in a pig model of myocardial infarction. The inclusion of cardiac fibroblasts in the cardiac patches enhanced the maturity and function of cardiac muscle cells. Transplantation of cardiac patches with cardiac fibroblasts resulted in better cardiac recovery and engraftment compared to patches without fibroblasts.
CARDIOVASCULAR RESEARCH
(2023)
Article
Cardiac & Cardiovascular Systems
Amit Gruber, Oded Edri, Shany Glatstein, Idit Goldfracht, Irit Huber, Gil Arbel, Amira Gepstein, Snizhanna Chorna, Lior Gepstein
Summary: This study aimed to develop high-resolution optogenetic approaches to modulate electrical activity in 2D and 3D cardiac tissue models derived from human induced pluripotent stem cell cardiomyocytes. The results demonstrated the ability to control the conduction and contractile properties of cardiac cells using optogenetics, and to use light-controllable techniques for cardiac resynchronization therapy and arrhythmia termination. This approach has important applications in cardiac physiology, pathophysiology, and arrhythmia treatment.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2022)
Article
Pharmacology & Pharmacy
Alina Constantin, Ioana Karla Comarita, Nicoleta Alexandru, Alexandru Filippi, Florina Bojin, Mihaela Gherghiceanu, Alexandra Vilcu, Miruna Nemecz, Loredan Stefan Niculescu, Virgil Paunescu, Adriana Georgescu
Summary: This study investigated the effects of stem cell-derived extracellular vesicles (EVs) on cardiac hypertrophy. The results showed that these EVs could reduce the expression of hypertrophic markers and molecules associated with inflammation in hypertrophic cardiomyocytes. The findings suggest that EVs have the potential to act as therapeutic mediators to reduce cardiac hypertrophy and subsequent cardiovascular events.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Cell Biology
Jun Li, Jong-Kook Lee, Keiko Miwa, Yuki Kuramoto, Kiyoshi Masuyama, Hideki Yasutake, Satoki Tomoyama, Hiroyuki Nakanishi, Yasushi Sakata
Summary: The study indicates that anisotropic fiber substrates can actively promote the development and maturation of hiPS-CMs, leading to more mature morphological and functional properties. While improvements diminish after withdrawal of structural support, the molecular developmental features are preserved.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Benjamin Theisen, Austin Holtz, Viswanathan Rajagopalan
Summary: Brugada Syndrome, a cardiac channelopathy, is associated with gene mutations and noncoding RNAs, leading to life-threatening arrhythmias. Studies have shown that the majority of loci associated with electrophysiological traits are located in noncoding regulatory regions, affecting gene expression dosage and cardiac ion channel function.
Article
Cardiac & Cardiovascular Systems
Hideki Kobayashi, Shugo Tohyama, Hideaki Kanazawa, Hajime Ichimura, Shuji Chino, Yuki Tanaka, Yota Suzuki, Jian Zhao, Naoko Shiba, Shin Kadota, Kazumasa Narita, Takafumi Naito, Tatsuichiro Seto, Koichiro Kuwahara, Yuji Shiba, Keiichi Fukuda
Summary: Advancements in stem cell biology have allowed for cardiac regeneration through the use of human pluripotent stem cell-derived cardiomyocytes (PSC-CMs). However, current transplantation methods involving PSC-CMs have limitations such as poor engraftment and the need for invasive procedures. This study investigated the feasibility of intracoronary transplantation of PSC-CMs in cynomolgus monkeys. The results showed low efficacy of this approach, with no coronary embolism or graft survival, and scar formation due to cardiac ischemic injury.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2023)
Article
Biology
Emiko Hayama, Yoshiyuki Furutani, Nanako Kawaguchi, Akiko Seki, Yoji Nagashima, Keisuke Okita, Daiji Takeuchi, Rumiko Matsuoka, Kei Inai, Nobuhisa Hagiwara, Toshio Nakanishi
Summary: The SCN5A R1623Q mutation is associated with severe congenital long QT syndrome 3 (LQT3) and may lead to enhanced sodium channel function. By establishing iPSC cardiomyocyte model and conducting pharmacological experiments, it is possible to evaluate responses to therapeutic drugs and potentially uncover the involvement of developmental switching of neonatal/adult Nav1.5 isoforms in the pathological mechanisms underlying severe long QT syndrome in fetuses and neonates.
Article
Cardiac & Cardiovascular Systems
Klaus Neef, Florian Drey, Vera Lepperhof, Thorsten Wahlers, Jurgen Hescheler, Yeong-Hoon Choi, Tomo Saric
Summary: Co-transplantation of iPS-CM and MSC improves cardiac regeneration after myocardial damage, demonstrating the potential of combining multiple cell types for increasing the efficacy of future cardiac cell therapies. MSCs enhance the retention of iPS-CM and result in a significantly higher left ventricular ejection fraction (LVEF) when transplanted together.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Review
Cell & Tissue Engineering
Pallavi Pushp, Diogo E. S. Nogueira, Carlos A. V. Rodrigues, Frederico C. Ferreira, Joaquim M. S. Cabral, Mukesh Kumar Gupta
Summary: Induced pluripotent stem cells (iPSCs) can be differentiated into cardiomyocytes for regenerative medicine, but require purification and maturation for in vivo applications. Recent advancements have been made in the generation and application of iPSC-derived CMs, as well as in the development of new small molecules for reprogramming and direct differentiation.
STEM CELL REVIEWS AND REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Yao Tan, Ying Chen, Tingting Lu, Nevin Witman, Bingqian Yan, Yiqi Gong, Xuefeng Ai, Li Yang, Minglu Liu, Runjiao Luo, Huijing Wang, Stefano Ministrini, Wei Dong, Wei Wang, Wei Fu
Summary: In this study, aligned electrospun rGO/PLCL membranes were prepared to mimic the structure of the human heart and human induced pluripotent stem cell-derived cardiomyocytes were assembled on these membranes to construct a myocardial muscle patch. The study found that the cells cultivated on the membranes exhibited an ordered and arranged structure, excellent mechanical properties, oxidation resistance, and effective guidance. Addition of rGO enhanced the maturation and synchronous electrical conductivity of the cardiomyocytes within the cardiac patch. This study verifies the potential use of conduction-consistent cardiac patches for drug screening and disease modeling applications.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Cell Biology
Ensieh Zahmatkesh, Mohammad Hossein Ghanian, Ibrahim Zarkesh, Zahra Farzaneh, Majid Halvaei, Zahra Heydari, Farideh Moeinvaziri, Amnah Othman, Marc Ruoss, Abbas Piryaei, Roberto Gramignoli, Saeed Yakhkeshi, Andreas Nussler, Mustapha Najimi, Hossein Baharvand, Massoud Vosough
Summary: Incorporating cell-sized tissue-specific microparticles (MPs) derived from liver extracellular matrix (ECM) into bioengineered liver organoids (BLOs) improved the maturation of human pluripotent stem cell-derived hepatocyte-like cells. This approach enhanced gene expression, function, CYP activities, albumin secretion, and xenobiotic metabolism of the hepatocyte-like cells. Furthermore, intraperitoneal transplantation of BLOs in a mouse model of acute liver injury increased survival rate, demonstrating the potential of this strategy for biomedical applications.
Article
Cell & Tissue Engineering
Tori Salem, Zachary Frankman, Jared M. Churko
Summary: This review explores important concepts and principles to consider when generating cardiovascular tissues from stem cell derived cardiomyocytes, as well as recent advancements in cardiac tissue engineering. Cardiac tissue engineering, involving complex engineering and physiological concepts, presents limitations in accessibility due to its interdisciplinary nature.
TISSUE ENGINEERING PART B-REVIEWS
(2022)
Article
Materials Science, Biomaterials
Cameron Best, Shuhei Tara, Matthew Wiet, James Reinhardt, Victoria Pepper, Matthew Ball, Tai Yi, Toshiharu Shinoka, Christopher Breuer
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2017)
Article
Materials Science, Biomaterials
Cameron Best, Shuhei Tara, Matthew Wiet, James Reinhardt, Victoria Pepper, Matthew Ball, Tai Yi, Toshiharu Shinoka, Christopher Breuer
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2017)
Article
Biophysics
Jason M. Szafron, Christopher K. Breuer, Yadong Wang, Jay D. Humphrey
JOURNAL OF BIOMECHANICAL ENGINEERING-TRANSACTIONS OF THE ASME
(2017)
Article
Medicine, Research & Experimental
Ekene Onwuka, Nakesha King, Eric Heuer, Christopher Breuer
COLD SPRING HARBOR PERSPECTIVES IN MEDICINE
(2018)
Article
Engineering, Biomedical
J. M. Szafron, R. Khosravi, J. Reinhardt, C. A. Best, M. R. Bersi, Tai Yi, C. K. Breuer, J. D. Humphrey
ANNALS OF BIOMEDICAL ENGINEERING
(2018)
Article
Cardiac & Cardiovascular Systems
Hideki Miyachi, James W. Reinhardt, Satoru Otsuru, Shuhei Tara, Hidetaka Nakayama, Tai Yi, Yong-Ung Lee, Shinka Miyamoto, Toshihiro Shoji, Tadahisa Sugiura, Christopher K. Breuer, Toshiharu Shinoka
INTERNATIONAL JOURNAL OF CARDIOLOGY
(2018)
Article
Otorhinolaryngology
Cameron A. Best, Victoria K. Pepper, Devan Ohst, Kyle Bodnyk, Eric Heuer, Ekene A. Onwuka, Nakesha King, Robert Strouse, Jonathan Grischkan, Christopher K. Breuer, Jed Johnson, Tendy Chiang
INTERNATIONAL JOURNAL OF PEDIATRIC OTORHINOLARYNGOLOGY
(2018)
Article
Medicine, Research & Experimental
Lauren Eichaker, Chengyu Li, Nakesha King, Victoria Pepper, Cameron Best, Ekene Onwuka, Eric Heuer, Kai Zhao, Jonathan Grischkan, Christopher Breuer, Jed Johnson, Tendy Chiang
Article
Cell & Tissue Engineering
Theresa Relation, Tai Yi, Adam J. Guess, Krista La Perle, Satoru Otsuru, Suheyla Hasgur, Massimo Dominici, Christopher Breuer, Edwin M. Horwitz
Article
Cell & Tissue Engineering
Takuma Fukunishi, Cameron A. Best, Chin Siang Ong, Tyler Groehl, James Reinhardt, Tai Yi, Hideki Miyachi, Huaitao Zhang, Toshiharu Shinoka, Christopher K. Breuer, Jed Johnson, Narutoshi Hibino
TISSUE ENGINEERING PART A
(2018)
Review
Cell & Tissue Engineering
Kevin M. Blum, Joseph D. Drews, Christopher K. Breuer
TISSUE ENGINEERING PART B-REVIEWS
(2018)
Article
Engineering, Biomedical
Sayali Dharmadhikari, Lumei Liu, Kimberly Shontz, Matthew Wiet, Audrey White, Andrew Goins, Himani Akula, Jed Johnson, Susan D. Reynolds, Christopher K. Breuer, Tendy Chiang
ACTA BIOMATERIALIA
(2020)
Review
Hematology
Thomas Breuer, Michael Jimenez, Jay D. Humphrey, Toshiharu Shinoka, Christopher K. Breuer
Summary: The research group has been using regenerative medicine strategies to develop improved biomaterials for congenital heart surgery for over 25 years. They have successfully developed a tissue-engineered vascular graft (TEVG) by combining tubular biodegradable polymeric scaffolds with autologous bone marrow-derived mononuclear cells. However, the first clinical trial revealed a high incidence of early TEVG stenosis, which hindered its widespread use. Through animal models and computational simulations, the group discovered the mechanism of TEVG stenosis and designed strategies to inhibit it.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
Tadahisa Sugiura, Goki Matsumura, Shinka Miyamoto, Hideki Miyachi, Christopher K. Breuer, Toshiharu Shinoka
SEMINARS IN THORACIC AND CARDIOVASCULAR SURGERY
(2018)
Article
Engineering, Biomedical
Tadahisa Sugiura, Riddhima Agarwal, Shuhei Tara, Tai Yi, Yong-Ung Lee, Christopher K. Breuer, Anthony S. Weiss, Toshiharu Shinoka
ACTA BIOMATERIALIA
(2017)