Article
Plant Sciences
M. Breygina, E. Klimenko, E. Shilov, A. Podolyan, A. Mamaeva, V. Zgoda, I. Fesenko
Summary: The study demonstrates that H2O2 in stigma exudate can cause hyperpolarization of plasma membrane in growing pollen tubes and pollen protoplasts, and this effect is inhibited by catalase and the ROS quencher MnTMPP. Proteomic analysis reveals alterations in protein composition related to cell metabolism and protein synthesis in H2O2-treated pollen grains. These findings suggest that H2O2 plays a role in stimulating various physiological processes in germinating pollen through activation of Ca2+- and K+-conducting channels.
Article
Plant Sciences
Maria Breygina, Oksana Luneva, Olga Schekaleva, Nadezhda Lazareva, Ksenia Babushkina, Igor A. Kirilyuk
Summary: This study investigated the release of ROS into the stigma exudate of various plants and its effect on pollen acceptance. The results showed that the total ROS level increased with stigma maturation in all plants studied. However, the concentration of hydrogen peroxide decreased in eudicots, except for peony. These findings reveal the primitive and progressive patterns of ROS generation and transformation during fertilization.
PLANT GROWTH REGULATION
(2023)
Article
Biochemistry & Molecular Biology
Maria Breygina, Dmitry Kochkin, Alexander Voronkov, Tatiana Ivanova, Ksenia Babushkina, Ekaterina Klimenko
Summary: This study investigated the composition of stigma exudates in tobacco and lily plants, and their effects on pollen germination. It was found that abscisic acid (ABA) was present in tobacco stigma exudate and had a stimulating effect on pollen germination. Analysis also showed differences in the fatty acid composition, with tobacco stigma exudate being more unsaturated compared to lily stigma exudate. Both exudates were found to contain squalene.
Article
Agronomy
Xue Luo, Ya-Nan Bai, Kai Sun, Wei Zhang, Chuan-Chao Dai
Summary: Long-term monocropping reduces flower formation, pollen viability, pollen germination, and tube growth, which decreases seed set in peanut. Genes associated with anther dehiscence, heat stress response, flavonoid biosynthesis, glutathione metabolism, and potassium uptake are enriched in flowers under monocropping, indicating water deficit and oxidative stress. Inhibition of reactive oxygen species synthesis or scavenging excess reactive oxygen species rescues pollen activity decline. Soil structure alterations, especially the reduction of large macro-aggregates (>2 mm), mediate flower water deficit and oxidative stress under long-term monocropping.
Editorial Material
Plant Sciences
Xuemei Zhou, Peng Zhao, Meng-Xiang Sun
Summary: Autophagy is a mechanism for degrading damaged or unwanted cells and recycling their components. Research on autophagy has expanded from yeast to mammals and plants, and the core machinery regulating autophagy appears to be conserved. In plants, autophagy plays essential roles in responses to stressful conditions and contributes to normal development, particularly in reproduction.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Agronomy
Francisco Garcia-Breijo, Jose Reig, Nuria Cebrian, Alfonso Garmendia, Roberto Beltran, Carlos Zornoza, Hugo Merle
Summary: Citrus species have a wet-type stigma with abundant exudate, and their style contains numerous canals. The main objective of the present study is to clarify the mode of action of sulfur on the ultrastructure of the Nadorcott mandarin stigma and style. The main results showed that sulfur specifically caused an alteration to the outer layer of stigma papillary cells, resulting in the prevention of pollen tube development inside the stigma.
Article
Plant Sciences
Katarzyna Roguz, Michal Chilinski, Agata Roguz, Marcin Zych
Summary: Pollination is essential for human food security and the reproduction of flowering plants. Urbanization and land use change affect plant-animal interactions, leading to concerns about pollination. A study in Warsaw, Poland found that the frequency of flower visits by pollinators was influenced by urban-related factors, but different groups of pollinators responded differently to these factors.
URBAN FORESTRY & URBAN GREENING
(2023)
Article
Plant Sciences
Rowan Herridge, Tyler McCourt, Jeanne M. E. Jacobs, Peter Mace, Lynette Brownfield, Richard Macknight
Summary: Self-incompatibility is a characteristic of flowering plants, and grasses have a unique two-locus system to control self-recognition. By analyzing genome data from ryegrass and other grass species, it was found that DUF247 genes and SP/ZP genes are present at the S and Z loci in self-incompatibility species, acting as male and female components. These genes are highly variable, but key secondary structure features are conserved. The time of formation of specificity groups is related to the divergence of grass subfamilies.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Horticulture
Meiyan Cui, Minh Duy Pham, Hyunseung Hwang, Changhoo Chun
Summary: The study shows that strawberry plants are affected by short-term high and low temperatures during flowering and fruit development, leading to abnormalities such as shortened flowering traits, delayed stigma maturation, and decreased pollen quality.
SCIENTIA HORTICULTURAE
(2021)
Article
Chemistry, Multidisciplinary
Zhifeng Liu, Shaobo Guo, Xun Fang, Xianzhao Shao, Zuoping Zhao
Summary: In this study, an FC antibacterial nano-agent was synthesized and its antibacterial activity and mechanism were investigated. The effects on mung bean development and toxicity to human mammary epithelial cells were also studied. The results showed that FC had strong antibacterial activity and low toxicity to human cells.
Article
Biochemistry & Molecular Biology
Maria Breygina, Alexander Voronkov, Tatiana Ivanova, Ksenia Babushkina
Summary: Pollen grains are unique haploid organisms with special composition and structure. Angiosperms and gymnosperms share similar mechanisms for pollen germination but gymnosperms exhibit slower growth rates and reduced reliance on female tissues. The properties of pollen lipids, especially very-long-chain FAs, contribute to these features. By analyzing the absolute content and FA composition of pollen lipids, significant differences were found between species, with spruce pollen exhibiting a higher proportion of saturated and monoene FAs. Lily and tobacco also showed variations in the FA composition between pollen coat and gametophyte cells, with a lower unsaturation index in the coat. Moreover, the absolute content of lipids was highest in lily pollen. This study provides new insights into the FA changes during pollen germination in gymnosperms and angiosperms, and highlights the impact of H2O2 on spruce pollen germination.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Environmental Sciences
Edward J. Calabrese, Evgenios Agathokleous
Summary: This paper evaluated the occurrence of hormetic dose responses in pollen reported over the past eight decades, finding a broad generality of this response across different taxa, inducing agents, and endpoints measured. This indicates the need to explore the emerging biological, ecological, agricultural, economic, and public health implications of inducing hormetic dose responses in pollen.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Review
Plant Sciences
Mohd Fadhli Hamdan, Shiu-Cheung Lung, Ze-Hua Guo, Mee-Len Chye
Summary: Acyl-CoA-binding proteins (ACBPs) play important roles in reproduction in plants and other organisms, potentially impacting crop improvement in agriculture.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Review
Biochemistry & Molecular Biology
Irina Georgiana Munteanu, Constantin Apetrei
Summary: This paper critically presents the most important tests used to determine antioxidant activity and their mechanisms, applicability, advantages and disadvantages. It covers tests based on the transfer of hydrogen atoms, electrons, and a combination of both, showcasing their efficacy in analyzing complex samples. Additionally, the paper discusses complementary methods using electrochemical biosensors to further understand the mechanisms and kinetics of antioxidants processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Neeta Lohani, Mohan B. Singh, Prem L. Bhalla
Summary: Global climate change is increasing the frequency of heatwaves, affecting the reproductive processes of Brassica napus and leading to yield loss. Short-term heat stress events not only impact pollen fitness but also impair the support function of the pistil, ultimately affecting seed set and yield. The study reveals that high temperature alters key regulatory pathways in reproductive tissues, providing insights for genetic improvement of thermotolerance and reproductive performance in B. napus varieties.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Maria Breygina, Denis V. Abramochkin, Nikita Maksimov, Igor Yermakov
FUNCTIONAL PLANT BIOLOGY
(2017)
Article
Plant Sciences
Nikita Maksimov, Anastasia Evmenyeva, Maria Breygina, Igor Yermakov
PLANT REPRODUCTION
(2018)
Article
Plant Sciences
M. Breygina, N. Maksimov, S. Polevova, A. Evmenyeva
Article
Plant Sciences
Alexandra Podolyan, Nikita Maksimov, Maria Breygina
JOURNAL OF PLANT PHYSIOLOGY
(2019)
Review
Biochemistry & Molecular Biology
Maria Breygina, Ekaterina Klimenko
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Plant Sciences
Alexandra Podolyan, Oksana Luneva, Ekaterina Klimenko, Maria Breygina
Summary: The differential modulation of ROS content in the microenvironment affects growth speed and morphology in lily pollen tubes. Oxygen radicals influence ionic zoning, membrane potential, and pH gradients, with O2•- considered as a growth stimulator and OH• as a negative regulator in pollen tube growth. Subcellular localization of different ROS impacts the growth and development of pollen tubes.
PLANT REPRODUCTION
(2021)
Article
Plant Sciences
Maria Breygina, Ekaterina Klimenko, Alexandra Podolyan, Alexander Voronkov
Article
Plant Sciences
M. Breygina, E. Klimenko, E. Shilov, A. Podolyan, A. Mamaeva, V. Zgoda, I. Fesenko
Summary: The study demonstrates that H2O2 in stigma exudate can cause hyperpolarization of plasma membrane in growing pollen tubes and pollen protoplasts, and this effect is inhibited by catalase and the ROS quencher MnTMPP. Proteomic analysis reveals alterations in protein composition related to cell metabolism and protein synthesis in H2O2-treated pollen grains. These findings suggest that H2O2 plays a role in stimulating various physiological processes in germinating pollen through activation of Ca2+- and K+-conducting channels.
Review
Plant Sciences
Maria Breygina, Ekaterina Klimenko, Olga Schekaleva
Summary: This article focuses on the physiological studies of conifer pollen, describing the main properties and diversity of pollen grains in gymnosperms, as well as their pollination strategies.
Article
Plant Sciences
Maria Breygina, Alexander Voronkov, Ilshat Galin, Guzel Akhiyarova, Svetlana Polevova, Ekaterina Klimenko, Igor Ivanov, Guzel Kudoyarova
Summary: This study used ELISA and immunolocalization techniques to investigate the changes in endogenous hormone levels and their localization in spruce pollen. The results showed significant fluctuations in hormone levels during pollen activation and polar growth, with variations in hormone localization between different cells.
Article
Biochemistry & Molecular Biology
Maria Breygina, Alexander Voronkov, Tatiana Ivanova, Ksenia Babushkina
Summary: Pollen grains are unique haploid organisms with special composition and structure. Angiosperms and gymnosperms share similar mechanisms for pollen germination but gymnosperms exhibit slower growth rates and reduced reliance on female tissues. The properties of pollen lipids, especially very-long-chain FAs, contribute to these features. By analyzing the absolute content and FA composition of pollen lipids, significant differences were found between species, with spruce pollen exhibiting a higher proportion of saturated and monoene FAs. Lily and tobacco also showed variations in the FA composition between pollen coat and gametophyte cells, with a lower unsaturation index in the coat. Moreover, the absolute content of lipids was highest in lily pollen. This study provides new insights into the FA changes during pollen germination in gymnosperms and angiosperms, and highlights the impact of H2O2 on spruce pollen germination.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Maria Breygina, Oksana Luneva, Olga Schekaleva, Nadezhda Lazareva, Ksenia Babushkina, Igor A. Kirilyuk
Summary: This study investigated the release of ROS into the stigma exudate of various plants and its effect on pollen acceptance. The results showed that the total ROS level increased with stigma maturation in all plants studied. However, the concentration of hydrogen peroxide decreased in eudicots, except for peony. These findings reveal the primitive and progressive patterns of ROS generation and transformation during fertilization.
PLANT GROWTH REGULATION
(2023)