Article
Developmental Biology
Zachary D. Root, David Jandzik, Cara Allen, Margaux Brewer, Marek Romasek, Tyler Square, Daniel M. Medeiros
Summary: The study reveals that the lectican gene family underwent independent duplications in lamprey, differing from gnathostomes. Despite this, the expression of lecticans in vertebrate-specific and mesenchyme-derived tissues is highly conserved. Lamprey expresses its lectican paralogs in distinct subpopulations of head skeleton precursors, hinting at a diversity of skeletal tissue types in the ancestral lineage.
DEVELOPMENTAL BIOLOGY
(2021)
Article
Endocrinology & Metabolism
Dylan J. J. Ritter, Dharmendra Choudhary, Gokhan Unlu, Ela W. W. Knapik
Summary: This study reveals that Rgp1 plays a crucial role in craniofacial cartilage development and the secretion of collagen II. Loss of Rgp1 leads to altered movement of Rab6a(+) vesicular compartments and activation of an Arf4b-mediated stress response in chondrocytes, resulting in cell death.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yaqin Ran, Wen Su, Lei Ma, Yunfei Tan, Zeng Yi, Xudong Li
Summary: This study successfully prepared keratin nanoparticles and found that keratin could influence the self-assembly process of collagen, forming exquisite fibrillar networks. The cell experiments showed that the CK composites exhibited better cellular affinity compared to collagen.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Wen Li, Naiwei Chi, Rathnayake A. C. Rathnayake, Rong Wang
Summary: The study found that a hybrid matrix of collagen I and collagen III can most effectively activate fibroblasts, promoting cell polarization and collagen synthesis. This is achieved through a fine balance between biochemical and biophysical cues on the hybrid matrix.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Review
Biochemistry & Molecular Biology
Ziwei Ouyang, Lei Dong, Feng Yao, Ke Wang, Yong Chen, Shufang Li, Renpeng Zhou, Yingjie Zhao, Wei Hu
Summary: Collagen plays an important role in maintaining the mechanical properties of articular cartilage and the stability of the extracellular matrix. The degradation of collagen is involved in the progressive destruction of cartilage in diseases such osteoarthritis and rheumatoid arthritis. Collagen degradation can generate new biochemical markers and collagen can also be used as a biomaterial with excellent properties. This review provides a comprehensive understanding of collagen and its role in cartilage repair, offering insights and techniques for clinical diagnosis and treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Genetics & Heredity
Allan J. Richards, Martin P. Snead
Summary: The fibrillar collagen family consists of major types I, II, III collagens and minor types V, XI. These collagens form heterotypic collagen fibrils that are regulated by the minor collagens, controlling their formation and size. Disruptions in the collagen biosynthesis process and alternative splicing can lead to various phenotypes. This article discusses the importance of considering these factors when analyzing DNA sequencing results from patients.
Review
Chemistry, Physical
Patrycja Ledwon, Anna Maria Papini, Paolo Rovero, Rafal Latajka
Summary: Collagen fibres degradation involves a variety of enzymes, with fibrillar collagens like type I, II, and III being responsible for tissue structure and skin elasticity. Understanding the enzymes involved in collagen degradation, especially those degrading fibrillar collagen, can be valuable for future research and applications.
Article
Engineering, Biomedical
Anais Dabbadie, Anna Salerno, Adam Perriman, Lu-Yun Lian, Anthony P. Hollander
Summary: This study found that the three modules of the collagen binding domain (CBD) of matrix metalloproteinase-2 (MMP-2) have specific biological properties that can be exploited for targeted stem cell therapy of cartilage lesions and other pathological sites.
Review
Engineering, Biomedical
Vishal Thomas, Jeremy Mercuri
Summary: This paper provides an in-depth investigation of the performance of naturally derived materials used in repairing/regenerating articular cartilage. While most natural constructs with native biomolecules have shown sub-optimal results in the past, current trends suggest that these materials have promising potential for effective scaffold fabrication.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Phoebe Szarek, David M. Pierce
Summary: Understanding the mechanical properties of collagen fibers and networks is crucial for studying biological tissues. This study aimed to establish methods for studying type II collagen fibers and networks, and determine the best practices for isolating and testing these networks in tension.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2022)
Article
Nanoscience & Nanotechnology
Yan Lu, Yuxiang Wang, Hanjie Zhang, Zizhao Tang, Xiaolin Cui, Xing Li, Jie Liang, Qiguang Wang, Yujiang Fan, Xingdong Zhang
Summary: The study demonstrated that functionalizing collagen scaffolds with porcine decellularized extracellular matrix can promote neocartilage regeneration. Results showed that the solution form of ECM performed better in cell recruitment, proliferation, and chondrogenesis, quickly providing a favorable chondrogenic microenvironment for endogenous BMSCs.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Food Science & Technology
Xianliang Luo, Wangxin Liu, Minjie Zhao, Tao Liu, Feifei Xiong, Lei Lei, Fuhuai Jia, Fengqin Feng
Summary: This study revealed that Atlantic salmon bone-derived collagen peptide (CPs) has potential in inhibiting osteoarthritis development and promoting anti-inflammatory and cartilage regeneration effects. CPs can reduce the expression of inflammatory factors, decrease chondrocytes apoptosis, and has good biocompatibility. However, further research is needed to explore the mechanism of CPs' positive effect on osteoarthritis.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Engineering, Biomedical
Benjamin J. Bielajew, Ryan P. Donahue, Elliott K. Lamkin, Jerry C. Hu, Vincent C. Hascall, Kyriacos A. Athanasiou
Summary: The objective of this study is to investigate the development of porcine cartilage by analyzing its mechanical properties, biochemical content, and proteomics at different developmental stages. The results show that the collagen content increases with age, while glycosaminoglycan and DNA content decrease. Tensile and compressive properties reach their peak in the juvenile stage. Proteomics analysis reveals changes in collagen composition and provides insight into the permanence of cartilage's matrix. Bottom-up proteomics techniques are critical for tissue characterization and tissue engineering efforts.
ACTA BIOMATERIALIA
(2022)
Article
Multidisciplinary Sciences
Max Kistler, Hannes Koehler, Jan Theopold, Ines Gockel, Andreas Roth, Pierre Hepp, Georg Osterhoff
Summary: This study investigates the reliability of hyperspectral imaging (HSI) in differentiating healthy and damaged cartilage tissue. The results show that the difference in absorbance at 540 nm can accurately identify damaged cartilage.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Medicinal
Eleonora Tassara, Boaz Orel, Micha Ilan, Dario Cavallo, Andrea Dodero, Maila Castellano, Silvia Vicini, Marco Giovine, Marina Pozzolini
Summary: This study investigates the molecular and chemical-physical properties of collagen extracted from Chondrosia reniformis sponges collected in different seasons to evaluate the potential impact of sea temperature. The results show that collagen fibrils extracted from samples at 17 degrees C have lower lysyl-hydroxylation level, thermal stability, and protein glycosylation level compared to those extracted from samples at 27 degrees C. Membranes made from 17 degrees C fibrils exhibit higher stiffness than those made from 27 degrees C fibrils. The differences in collagen properties are important in guiding the use of the biomaterial.
Article
Biology
David Jandzik, David W. Stock
Summary: This study provides evidence for the influence of external or internal factors on morphological diversification during adaptive radiation, focusing on dental diversity in characiform fishes. Results suggest that differences in evolvability between lineages may lead to contrasting patterns of diversification, with implications for the retention of ancestral developmental potential.
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2021)
Article
Zoology
Daniel Jablonski, Marco Antonio Ribeiro-Junior, Shai Meiri, Erez Maza, Oleg Kukushkin, Marina Chirikova, Angelika Pirosova, Dusan Jelic, Peter Mikulicek, David Jandzik
Summary: The Levant is an important hotspot for reptile diversity and endemism, with Pseudopus apodus being a prominent member of the herpetofauna in the region. A new subspecies of Pseudopus apodus is described in the Levant, genetically diverse and forming a sister lineage to the populations in other parts of its range according to mitochondrial DNA. This new subspecies is the largest-bodied but has the smallest range among the three subspecies.
VERTEBRATE ZOOLOGY
(2021)
Article
Developmental Biology
Zachary D. Root, David Jandzik, Cara Allen, Margaux Brewer, Marek Romasek, Tyler Square, Daniel M. Medeiros
Summary: The study reveals that the lectican gene family underwent independent duplications in lamprey, differing from gnathostomes. Despite this, the expression of lecticans in vertebrate-specific and mesenchyme-derived tissues is highly conserved. Lamprey expresses its lectican paralogs in distinct subpopulations of head skeleton precursors, hinting at a diversity of skeletal tissue types in the ancestral lineage.
DEVELOPMENTAL BIOLOGY
(2021)
Review
Biodiversity Conservation
Zachary D. Root, Claire Gould, Margaux Brewer, David Jandzik, Daniel M. Medeiros
Summary: Jawed vertebrates have been dominant for hundreds of millions of years, with jawless vertebrates providing important insights into vertebrate evolution. Cyclostomes, such as lampreys and hagfishes, play a critical role in understanding the stepwise acquisition of vertebrate chondroid tissues. Despite differences in skeletal morphology, there is a conserved genetic regulation among living vertebrates.
Article
Zoology
Daniel Jablonski, Neftali Sillero, Oleksandra Oskyrko, Adriana Bellati, Andris Ceirans, Marc Cheylan, Dan Cogalniceanu, Jelka Crnobrnja-Isailovic, Pierre-Andre Crochet, Angelica Crottini, Igor Doronin, Georg Dzukic, Philippe Geniez, Cetin Ilgaz, Ruben Iosif, David Jandzik, Dusan Jelic, Spartak Litvinchuk, Katarina Ljubisavljevic, Petros Lymberakis, Peter Mikulicek, Edvard Mizsei, Jiri Moravec, Bartlomiej Najbar, Maciej Pabijan, Mihails Pupins, Patricia Sourrouille, Ilias Strachinis, Marton Szabolcs, Evanthia Thanou, Elias Tzoras, Vladislav Vergilov, Judit Voros, Vaclav Gvozdik
Summary: The slow-worm lizards comprise five species with relatively uniform morphology but deep genetic divergence. Four of the species live in parapatry, while only two species occur in partial sympatry in certain regions. Further research is needed to explore the eco-evolutionary interactions between these species, including their hybridization rates, to uncover more details about the slow-worm evolutionary and natural history.
Article
Biochemistry & Molecular Biology
Weizhao Yang, Nathalie Feiner, Daniele Salvi, Hanna Laakkonen, Daniel Jablonski, Catarina Pinho, Miguel A. Carretero, Roberto Sacchi, Marco A. L. Zuffi, Stefano Scali, Konstantinos Plavos, Panayiotis Pafilis, Nikos Poulakakis, Petros Lymberakis, David Jandzik, Ulrich Schulte, Fabien Aubret, Arnaud Badiane, Guillem Perez I de Lanuza, Javier Abalos, Geoffrey M. While, Tobias Uller
Summary: The study reveals the evolutionary history and genetic structure of common wall lizards using population genomic and phylogenomic analyses, identifying six major lineages and demonstrating the significant impact of Mediterranean geology and climate on the evolutionary history and population genetic structure of extant species.
MOLECULAR BIOLOGY AND EVOLUTION
(2022)
Article
Cell Biology
Jan Stundl, Vladimir Soukup, Roman Franek, Anna Pospisilova, Viktorie Psutkova, Martin Psenicka, Robert Cerny, Marianne E. Bronner, Daniel Meulemans Medeiros, David Jandzik
Summary: In the last decade, CRISPR/Cas9 has been proven to be an effective and efficient method for targeted mutagenesis in genetic model systems. Its high efficiency allows the immediate generation of near-null mosaic mutants, making it valuable for organisms with life histories that preclude the establishment of purebred genetic lines. In the study, the successful use of CRISPR/Cas9 mutagenesis in a non-teleost fish, sterlet Acipenser ruthenus, was reported, indicating the potential for applying this technology to other long-lived, slow-maturing aquatic vertebrates.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
M. Brent Hawkins, David Jandzik, Frank J. Tulenko, Amanda N. Cass, Tetsuya Nakamura, Neil H. Shubin, Marcus C. Davis, David W. Stock
Summary: By using chemical genetics, researchers have demonstrated a mutually agonistic interaction between Fgf and Shh genes in the developing dorsal fin of the channel catfish. They also found similar gene expression patterns in median fins of representatives from other major vertebrate lineages, supporting a median fin-first scenario for vertebrate paired appendage origins.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Vaclav Gvozdik, Tadeas Necas, Daniel Jablonski, Emily Moriarty Lemmon, Alan R. Lemmon, David Jandzik, Jiri Moravec
Summary: A study revises the dated phylogenetic hypothesis on the evolutionary history of Western Palaeartic lizards using nuclear DNA and mitogenomes, finding overall concordance between the two methods except for A. veronensis. Mitochondrial DNA suggests a common clade between A. veronensis and A. cephallonica, while nuclear DNA clusters A. veronensis with A. fragilis. The discordance is explained by ancient mitochondrial capture in a geographically restricted population during the Messinian event. The study also provides dating estimates for the divergence of different lizard species.
MOLECULAR PHYLOGENETICS AND EVOLUTION
(2023)
Article
Biology
Agata Horackova, Anna Pospisilova, Jan Stundl, Martin Minarik, David Jandzik, Robert Cerny
Summary: In this study, we analyzed the expression patterns of selected core pharyngeal regulatory network genes in bichir and sturgeon embryos and found pharyngeal pouch-like outpocketings in the pre-oral domain. These structures, known as pre-mandibular pharyngeal pouches, exhibit similar morphogenesis and gene expression patterns to post-oral pharyngeal pouches. Our findings suggest that pre-mandibular pharyngeal pouches represent an ancestral vertebrate trait inherited from the pharyngeal metameric organization of our ancestors.
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2023)
Correction
Ecology
Francesco Lamanna, Francisca Hervas-Sotomayor, A. Phillip Oel, David Jandzik, Daniel Sobrido-Camean, Gabriel N. Santos-Duran, Megan L. Martik, Jan Stundl, Stephen A. Green, Thoomke Bruening, Katharina Moessinger, Julia Schmidt, Celine Schneider, Mari Sepp, Florent Murat, Jeramiah J. Smith, Marianne E. Bronner, Maria Celina Rodicio, Anton Barreiro-Iglesias, Daniel M. Medeiros, Detlev Arendt, Henrik Kaessmann
NATURE ECOLOGY & EVOLUTION
(2023)
Article
Ecology
Francesco Lamanna, Francisca Hervas-Sotomayor, A. Phillip Oel, David Jandzik, Daniel Sobrido-Camean, Gabriel N. Santos-Duran, Megan L. Martik, Jan Stundl, Stephen A. Green, Thoomke Bruening, Katharina Moessinger, Julia Schmidt, Celine Schneider, Mari Sepp, Florent Murat, Jeramiah J. Smith, Marianne E. Bronner, Maria Celina Rodicio, Anton Barreiro-Iglesias, Daniel M. Medeiros, Detlev Arendt, Henrik Kaessmann
Summary: By studying the sea lamprey, researchers have created a comprehensive cell type atlas of the primitive vertebrate brain, which has revealed the cellular and molecular architecture of the ancestral vertebrate brain and identified key tissues and cell types that arose later in evolution.
NATURE ECOLOGY & EVOLUTION
(2023)