Article
Biophysics
Zaobing Zhu, Yongkun Guo, Chen Wang, Zifeng Yang, Rong Li, Zhiqi Zeng, Hui Li, Dabing Zhang, Litao Yang
Summary: The article introduces a sensitive and rapid diagnosis method called OPERATOR, which utilizes enzyme-catalyzed rolling circle amplification-assisted CRISPR/FnCas12a assay for detecting SARS-CoV-2. OPERATOR presents advantages of ultra-sensitivity, high specificity, rapid reaction speed, easy operation, low cost, and on-spot visualization. It can be combined with rapid RNA release and a lateral flow strip for point-of-care testing.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Sitong Liu, Tie Xie, Zhaohe Huang, Xiaojing Pei, Shujing Li, Yifan He, Yigang Tong, Guoqi Liu
Summary: The CRISPR/Cas system has been widely used in molecular diagnostics, especially during the COVID-19 outbreak. In this study, we comprehensively investigated various types of fluorescent reporters for Cas12a and found that molecular beacons (MB) outperformed single-strand DNA (ssDNA) and double-strand DNA (dsDNA) reporters in terms of analytical performance. Using MB reporters, we successfully developed a highly sensitive SARS-CoV-2 detection method without the need for amplification strategy. Our findings provide new perspectives for simple, highly-sensitive, and universal molecular diagnosis in various applications.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Sitong Liu, Tie Xie, Xiaojing Pei, Shujing Li, Yifan He, Yigang Tong, Guoqi Liu
Summary: A universal, rapid, sensitive, and specific POC test for SARS-CoV-2 detection was developed using Cas12a DNase activity and AuNPs strand-displacement probe. The color change of released AuNPs can be visually detected and quantified using a UV-Vis spectrometer. The method has potential for POC diagnosis of various nucleic acid targets.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Biochemistry & Molecular Biology
Rong Zhao, Wang Luo, You Wu, Li Zhang, Xin Liu, Junjie Li, Yujun Yang, Li Wang, Luojia Wang, Xiaole Han, Zhongzhong Wang, Jianhong Zhang, Ke Lv, Tingmei Chen, Guoming Xie
Summary: In this article, a CRISPR/Cas12a regulation strategy based on nucleic acid nanotechnology is proposed. By adjusting external RNA accessories, the performance of CRISPR/Cas12a can be finely controlled. This strategy has flexibility and versatility, and holds great potential for deeper applications of CRISPR technology in various fields.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biophysics
Xiao-Min Hang, Hui-Yi Wang, Peng-Fei Liu, Kai-Ren Zhao, Li Wang
Summary: This study reported a fast and accurate method for detecting SARS-CoV-2 infection, combining RTF-EXPAR and CRISPR-Cas12a technologies. This method has high sensitivity and specificity, and requires minimal equipment, making it suitable for rapid on-site testing.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Immunology
Cristina Ramirez-Cordova, Diana Morales-Jadan, Sofia Alarcon-Salem, Alisson Sarmiento-Alvarado, Maria Belen Proano, Isabel Camposano, Berenice Sarmiento-Alvarado, Mishell Bravo-Castro, Jean Franco Hidalgo-Jimenez, Dayana Coello, Angel S. Rodriguez, Carolina Viteri-Davila, Alexander Paolo Vallejo-Janeta, Daniela Arcos-Suarez, Miguel Angel Garcia-Bereguiain
Summary: This study evaluated a simple SARS-CoV-2 detection method based on direct RT-qPCR after sample homogenization. The results showed that this method has high sensitivity for potentially infectious SARS-CoV-2 positive patients, and it can reduce diagnosis time and cost, and overcome the shortage of RNA extraction kits during the COVID-19 pandemic.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Reza Nouri, Yuqian Jiang, Zifan Tang, Xiaojun Lance Lian, Weihua Guan
Summary: A novel SARS-CoV-2 detection strategy, integrating a solid-state CRISPR-Cas12a-assisted nanopore sensing approach, was developed with a nanopore-sized counting method and a kinetic cleavage model to achieve precise detection of viral RNA, demonstrating trade-offs between sensitivity, specificity, and detection limit.
Article
Chemistry, Analytical
Peipei Qin, Pinru Chen, Nan Deng, Liu Tan, Bin-Cheng Yin, Bang-Ce Ye
Summary: This study proposes a method to directly regulate the trans cleavage activity of Cas12a using an allosteric DNA probe, enabling the sensing of different types of analytes. The proposed strategy utilizes an allosteric inhibitor probe that can induce a change in conformation and remove its inhibitory function on Cas12a upon binding to a specific target. The method was validated for detecting adenosine triphosphate and let-7a, demonstrating excellent specificity.
ANALYTICAL CHEMISTRY
(2022)
Article
Microbiology
Yuanhao Liang, Hongqing Lin, Lirong Zou, Jianhui Zhao, Baisheng Li, Haiying Wang, Jing Lu, Jiufeng Sun, Xingfen Yang, Xiaoling Deng, Shixing Tang
Summary: This study developed a CRISPR-Cas12-based system for the direct detection of SARS-CoV-2 variants, achieved high sensitivity, and successfully distinguished specific mutations in different variants. The approach has the potential for rapid development and implementation in resource-limited settings.
MICROBIOLOGY SPECTRUM
(2021)
Article
Biochemistry & Molecular Biology
Marianna Rossetti, Rosa Merlo, Neda Bagheri, Danila Moscone, Anna Valenti, Aakash Saha, Pablo R. Arantes, Rudy Ippodrino, Francesco Ricci, Ida Treglia, Elisabetta Delibato, John van der Oost, Giulia Palermo, Giuseppe Perugino, Alessandro Porchetta
Summary: By using hairpin DNA as FRET-based reporters, we have enhanced the trans-cleavage activity of Cas12a enzymes, allowing for faster detection of clinically relevant double stranded DNA targets.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Nanoscience & Nanotechnology
Lina Wu, Xinjie Wang, Xiangchuan Wu, Shiqi Xu, Ming Liu, Xizhong Cao, Taishan Tang, Xingxu Huang, He Huang
Summary: The CRISPR-Cas system has been developed into a molecular diagnostic tool with high sensitivity, low cost, and high specificity. This study demonstrates the use of a MnO2 nanozyme-mediated CRISPR-Cas12a system for the detection of SARS-CoV2. The system shows high selectivity and facile operation, making it a promising molecular diagnosis tool for point-of-care testing.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Multidisciplinary
Fengqin Li, Junru Li, Weiqiang Yang, Shuai Yang, Congshuo Chen, Ling Du, Junyang Mei, Qianyun Tang, Xiaojing Chen, Chi Yao, Dayong Yang, Xiaolei Zuo, Peifeng Liu
Summary: The trans-cleavage property of CRISPR-Cas12a system is utilized for disease diagnosis. The effect of different local densities of Framework-Hotspot reporters (FHRs) on the cleavage activity of Cas12a is investigated. A modular sensing platform is constructed with target recognition by CRISPR-Cas12a and signal transduction by FHR. This platform enables sensitive and rapid detection of pathogen nucleic acids and tumor protein markers without pre-amplification.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Multidisciplinary Sciences
Natacha S. Ogando, Priscila El Kazzi, Jessika C. Zevenhoven-Dobbe, Brenda W. Bontes, Alice Decombe, Clara C. Posthuma, Volker Thiel, Bruno Canard, Francois Ferron, Etienne Decroly, Eric J. Snijder
Summary: The study found that N7-MTase is a critical enzyme for betacoronavirus replication and essential for virus viability. By conducting mutagenesis experiments on betacoronavirus nonstructural protein 14, key residues of the catalytic pocket were identified, which could be targeted for designing inhibitors with potential pan-coronaviral activity spectrum.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Christopher A. Beaudoin, Arian R. Jamasb, Ali F. Alsulami, Liviu Copoiu, Andries J. van Tonder, Sharif Hala, Bridget P. Bannerman, Sherine E. Thomas, Sundeep Chaitanya Vedithi, Pedro H. M. Torres, Tom L. Blundell
Summary: The study explores the potential mimicry of receptor-binding motifs on the spike proteins of three coronaviruses, revealing similarities to both human and non-human proteins. This indicates pathways utilized by the spike proteins and possible shared host interactions, guiding experimental efforts to understand interactions between human and coronavirus proteins.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Article
Chemistry, Analytical
Riham Zayani, Dorra Rezig, Wasfi Fares, Mouna Marrakchi, Makram Essafi, Noureddine Raouafi
Summary: The study developed a magnetofluorescent bioplatform for the direct and specific detection of SARS-CoV-2 viral RNA in COVID-19-positive patients' nasopharyngeal swabs with high selectivity and sensitivity. It was able to differentiate SARS-CoV-2 RNA from other related viruses and demonstrated 100% sensitivity and specificity in clinical samples.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Yen-Hung Lin, Yang Han, Abhinav Sharma, Wejdan S. AlGhamdi, Chien-Hao Liu, Tzu-Hsuan Chang, Xi-Wen Xiao, Wei-Zhi Lin, Po-Yu Lu, Akmaral Seitkhan, Alexander D. Mottram, Pichaya Pattanasattayavong, Hendrik Faber, Martin Heeney, Thomas D. Anthopoulos
Summary: A novel biosensor transistor concept utilizing a solution-processed In2O3/ZnO semiconducting heterojunction with a three-channel architecture has been developed for real-time detection of biomolecules. Functionalizing the channel surface enables rapid detection of SARS-CoV-2, demonstrating significant potential for emerging bioanalytical applications.
ADVANCED MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Zhongli Wang, Mengyuan Gao, Chunyong He, Weichao Shi, Yunfeng Deng, Yang Han, Long Ye, Yanhou Geng
Summary: This study investigates the aggregation structure of a high-mobility conjugated copolymer and demonstrates its dependence on the molar mass. The researchers were able to prepare a highly aligned and ordered film using the ordered packing of the aggregated structure in solution, leading to a reliable hole mobility in organic thin-film transistors. The results provide insights into the microstructure control of high-performance electronic devices.
ADVANCED MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Xiaoyu Liu, Yibo Shi, Qian Zhou, Jie Liu, Lang Jiang, Yang Han
Summary: The study focused on fabricating n-type organic thin film transistors (OTFTs) using a blend of polystyrene and small molecule DPP1012-4F under atmospheric conditions to improve solution processibility and air stability. The addition of high-boiling point solvent additive 1-chloronaphthalene reduced grain boundary defects in the microscopic morphology. The results showed a high electron mobility of 0.73 cm(2)center dot V-1 center dot s(-1), demonstrating the potential of this blending strategy for industrial production of high-mobility n-type OTFTs.
CHINESE JOURNAL OF CHEMISTRY
(2022)
Article
Energy & Fuels
Amr S. Zalhaf, Ensheng Zhao, Yang Han, Ping Yang, Abdulrazak H. Almaliki, Reda M. H. Aly
Summary: This work evaluates the impact of lightning strikes on DC transmission lines using the Yunnan-Guizhou 500 kV HVDC transmission system as a case study. The research investigates various technical aspects that have not been previously studied in detail, including the back-flashover and shielding-failure phenomena. Simulation results show that increasing the lightning current peak and grounding resistance leads to higher overvoltages and an increased probability of back-flashover. The study also examines the effect of changing lightning strike parameters on shielding-failure flashover.
Article
Engineering, Multidisciplinary
Yangyang Han, Changlin Ma, Shengjin Tang, Fengfei Wang, Xiaoyan Sun, Xiaosheng Si
Summary: The paper proposes a heuristic algorithm and an unbiased parameter estimation method for residual life estimation based on the nonlinear Wiener process with measurement error. Experimental results demonstrate the effectiveness of these methods.
PROCEEDINGS OF THE INSTITUTION OF MECHANICAL ENGINEERS PART O-JOURNAL OF RISK AND RELIABILITY
(2023)
Article
Energy & Fuels
Amr S. Zalhaf, Yang Han, Ping Yang, Congling Wang, Muhammad Ammar Khan
Summary: This study investigates the transient behavior of wind farm substations during lightning strikes, analyzing the lightning behavior of substations with different designs. The lightning surge overvoltages caused by back flashover and shielding-failure are calculated, providing important insights for the design of lightning protection systems for wind farm substations connected to overhead transmission lines.
Article
Energy & Fuels
Ibrahim Hetita, Amr S. Zalhaf, Diaa-Eldin A. Mansour, Yang Han, Ping Yang, Congling Wang
Summary: This study introduces a new method using the PEEC method to establish a high-precision model of the PV system, considering the coupling effects and the influence of the metal frame. Simulation results show that increasing soil resistivity values lead to an increase in transient overvoltages, while the presence of a metal frame increases the induced overvoltage values, changing the requirements for lightning protection systems.
Article
Engineering, Electrical & Electronic
Ensheng Zhao, Yang Han, Xiangyang Lin, Ping Yang, Frede Blaabjerg, Amr S. Zalhaf
Summary: This paper establishes a complete impedance model of a two-stage PV inverter in order to obtain impedance characteristics and reveal stability issues when connected to a weak grid. The model includes PV arrays, front-end Boost converter, and rear-end inverter with output LCL filter. It is found that the DC output impedance of the front-end circuit behaves as a first-order low-pass filter, mainly influenced by the DC-link capacitor. Additionally, a decrease in the integral gain of the outer voltage loop can cause negative resistance and capacitive impedance in the D-axis impedance component, affecting system stability. The stability issues of the PV inverter connected to a weak grid are evaluated using the impedance analysis method with different divisions of source-load subsystems. It is demonstrated that increasing the PLL bandwidth and decreasing the integral gain of the outer voltage loop both lead to oscillation, and increasing the output capacity deteriorates stability margin.
ELECTRIC POWER SYSTEMS RESEARCH
(2022)
Article
Green & Sustainable Science & Technology
Siyu Zhou, Yang Han, Ping Yang, Karar Mahmoud, Matti Lehtonen, Mohamed M. F. Darwish, Amr S. Zalhaf
Summary: This paper proposes an optimal network constraint-based expansion planning model to improve the topological flexibility in modern distribution networks. The model integrates intermittent renewable energy resources and considers uncertainty-based multiple scenarios for enhanced robustness and reliability. The proposed model takes into account the best investment locations and times of substations, lines, and renewable energy-based distributed generations.
Article
Engineering, Electrical & Electronic
Ensheng Zhao, Yang Han, Xiangyang Lin, Enping Liu, Ping Yang, Amr S. Zalhaf
Summary: This research investigates the harmonic characteristics of a photovoltaic system connected to a weak grid. The study constructs a passive impedance network using the impedance model of a PV inverter in the positive and negative sequence coordinate system. The amplification coefficient of the harmonic voltage at the point of common coupling is obtained by analyzing the series resonance of the impedance network. The research also proposes a harmonic mitigation control strategy to solve the problem of excessive output harmonics under weak grid background harmonic conditions.
INTERNATIONAL JOURNAL OF ELECTRICAL POWER & ENERGY SYSTEMS
(2022)
Article
Engineering, Electrical & Electronic
Ensheng Zhao, Yang Han, Yuxiang Liu, Ping Yang, Amr S. Zalhaf, Frede Blaabjerg
Summary: This paper proposes a multi-damping coefficient design method for PBC controllers based on stability domain analysis for SPVSI systems with LC filters. The designed controller ensures system stability under various load conditions.
INTERNATIONAL JOURNAL OF ELECTRICAL POWER & ENERGY SYSTEMS
(2023)
Letter
Cell Biology
Chuan-Ping Zhang, Hou-Yuan Qiu, Cai-Xiang Zhang, Yu-Ming Zhang, Yi-Zhou Zhang, Hao Yin, Ke-Qin Zhang, Ying Zhang
JOURNAL OF MOLECULAR CELL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Xuehong Qian, Lingang He, Jiejie Yang, Jiajia Sun, Xueying Peng, Yuting Zhang, Yizhou Mao, Ying Zhang, Yixian Cui
Summary: In this study, we discovered that the autophagy regulator UVRAG plays a crucial role in orchestrating the assembly of ER-phagy sites (ERPHS). UVRAG localizes to ERPHs and interacts with specific ER-phagy cargo receptors, regulating their oligomerization and facilitating the recruitment of core autophagy proteins.
Article
Engineering, Biomedical
Jing An, Chuan-Ping Zhang, Hou-Yuan Qiu, Hong-Xia Zhang, Qiu-Bing Chen, Yu-Ming Zhang, Xin-Lin Lei, Cai-Xiang Zhang, Hao Yin, Ying Zhang
Summary: An isotonic buffer that reduces cytosolic cGAS-DNA interactions improves the viability of genome-engineered T cells transfected by electroporation.
NATURE BIOMEDICAL ENGINEERING
(2023)
Article
Engineering, Electrical & Electronic
Amr S. Zalhaf, Diaa-Eldin A. Mansour, Yang Han, Ping Yang, Mohamed M. F. Darwish
Summary: This article studies the transient behavior of wind turbines when two blades are struck simultaneously by lightning. A simplified numerical model is used to calculate the variation of induced overvoltages across the turbines during the strike. To validate the numerical model, a small-scale wind turbine model is connected with an impulse generator for laboratory simulation, and the measured overvoltages closely match the numerical results.
IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT
(2022)
