Transcriptional Regulation of Reproductive Diapause in the Convergent Lady Beetle, Hippodamia convergens

Simple Summary Diapause is a dormant period typically controlled by daylength that ensures an insect's survival through harsh environmental conditions. The convergent lady beetle, Hippodamia convergens, undergoes a reproductive diapause in winter, where female ovaries remain immature and no eggs are laid. This species is an important biological control agent, but during diapause, beetles are less likely to eat pest insects. Thus, knowledge of diapause mechanisms may facilitate manipulation thereof to improve biological control. Further, molecular studies of adult diapause and diapause in Coleoptera are relatively lacking. Here, we assembled and annotated a transcriptome for this species and quantified transcript expression changes during diapause. Female beetles were sampled at three times in diapause (early, mid, and late diapause), which allowed us to characterize the molecular processes occurring at distinct transitions throughout diapause. We found that transcripts involved in flight were consistently upregulated during diapause, which is consistent with dispersal flights at this stage, while transcripts involved in ovarian development were downregulated, which is consistent with the shutdown of reproduction in diapausing females. These findings identify key regulators of diapause in H. convergens and contribute to a growing body of literature on the molecular mechanisms of diapause across the insect phylogeny. Diapause is an alternate development program that synchronizes an insect's life cycle with seasonally abundant resources and ensures survival in unfavorable conditions. The physiological basis of diapause has been well characterized, but the molecular mechanisms regulating it are still being elucidated. Here, we present a de novo transcriptome and quantify transcript expression during diapause in the convergent lady beetle Hippodamia convergens. H. convergens is used as an augmentative biocontrol agent, and adult females undergo reproductive diapause that is regulated by photoperiod. We sampled females at three stages (early, mid, and late diapause) and compared transcript expression to non-diapausing individuals. Based on principle component analysis, the transcriptomes of diapausing beetles were distinct from non-diapausing beetles, and the three diapausing points tended to cluster together. However, there were still classes of transcripts that differed in expression across distinct phases of diapause. In general, transcripts involved in muscle function and flight were upregulated during diapause, likely to support dispersal flights that occur during diapause, while transcripts involved in ovarian development were downregulated. This information could be used to improve biological control by manipulating diapause. Additionally, our data contribute to a growing understanding of the genetic regulation of diapause across diverse insects.

Automated summary

Lady beetles undergo reproductive diapause in winter, where females do not lay eggs, and transcriptome analysis shows that genes related to flight are consistently upregulated, while genes involved in ovarian development are downregulated, contributing to the survival strategy during diapause.