Oxidative stress-induced endothelial dysfunction and decreased vascular nitric oxide in COVID-19 patients

Background SARS-CoV-2 targets endothelial cells through the angiotensin-converting enzyme 2 receptor. The resulting endothelial injury induces widespread thrombosis and microangiopathy. Nevertheless, early specific markers of endothelial dysfunction and vascular redox status in COVID-19 patients are currently missing.& nbsp;Methods Observational study including ICU and non-ICU adult COVID-19 patients admitted in hospital for acute respiratory failure, compared with control subjects matched for cardiovascular risk factors similar to ICU COVID-19 patients, and ICU septic shock patients unrelated to COVID-19.& nbsp;Findings Early SARS-CoV-2 infection was associated with an imbalance between an exacerbated oxidative stress (plasma peroxides levels in ICU patients vs. controls: 1456.0 +/- 400.2 vs 436 +/- 272.1 mmol/L; P < 0.05) and a reduced nitric oxide bioavailability proportional to disease severity (5-a-nitrosyl-hemoglobin, HbNO in ICU patients vs. controls: 116.1 +/- 62.1 vs. 163.3 +/- 46.7 nmol/L; P < 0.05). HbNO levels correlated with oxygenation parameters (PaO2/FiO(2) ratio) in COVID-19 patients (R-2 = 0.13; P < 0.05). Plasma levels of angiotensin II, aldosterone, renin or serum level of TREM-1 ruled out any hyper-activation of the renin-angiotensin-aldosterone system or leucocyte respiratory burst in ICU COVID-19 patients, contrary to septic patients.& nbsp;Interpretation Endothelial oxidative stress with ensuing decreased NO bioavailability appears as a likely pathogenic factor of endothelial dysfunction in ICU COVID-19 patients. A correlation between NO bioavailability and oxygenation parameters is observed in hospitalized COVID-19 patients. These results highlight an urgent need for oriented research leading to a better understanding of the specific endothelial oxidative stress that occurs during SARS-CoV-2.& nbsp;Copyright (C) 2022 The Author(s). Published by Elsevier B.V.

Automated summary

SARS-CoV-2 infection can cause increased oxidative stress and decreased nitric oxide bioavailability in ICU COVID-19 patients, which may contribute to endothelial dysfunction. Nitric oxide bioavailability is correlated with oxygenation parameters in hospitalized COVID-19 patients.