Article
Chemistry, Multidisciplinary
Shamsul Abedin, Yong Li, Abid Anjum Sifat, Khokan Roy, Eric O. Potma
Summary: This study addresses the challenges of stability and reproducibility in surface-enhanced coherent anti-Stokes Raman scattering (SE-CARS) by using nanoantennas made from high-index dielectric particles. The experimental results demonstrate enhanced molecular CARS signals observed at Si nanoantennas, which offer improved thermal stability compared to metallic counterparts.
Article
Chemistry, Organic
Ervin Kovacs, Levente Cseri, Attila Jancso, Ferenc Terenyi, Anna Fulop, Balazs Rozsa, Gabor Galbacs, Zoltan Mucsi
Summary: Novel small-molecular analogues of the green fluorescence protein (GFP) chromophore were synthesised to expand and improve this fluorophore family and deepen the understanding of their fluorescence mechanism. The introduction of specific substituents and manipulation of the molecular structure successfully enhanced fluorescence emission intensity and altered spectral properties.
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2021)
Article
Physics, Applied
Zeeshan H. Amber, Benjamin Kirbus, Lukas M. Eng, Michael Ruesing
Summary: Thin-film lithium niobate (TFLN) on insulator in x- or z-cut form has shown potential for integrated optoelectronic devices, with optical second-harmonic generation (SHG) microscopy providing insights into its structural properties. SHG in TFLN is influenced by interfacial reflections and resonant enhancements, with the dominant SHG contribution being the co-propagating phase-matched signal. The coherent interaction length lc depends on the incident laser wavelength and numerical aperture of the focusing objective.
JOURNAL OF APPLIED PHYSICS
(2021)
Article
Chemistry, Analytical
Georgi Petrov, Rajan Arora, Vladoslav V. Yakovlev
Summary: The study demonstrated chemical imaging of calcifications in tissues using long wavelength excitation, with imaging and analysis performed 2 mm below the skin level in a model system. Applications to breast cancer diagnostics and imaging were discussed, along with methods to extend depth and improve spatial resolution in chemical imaging.
Article
Instruments & Instrumentation
Nao Hirai, Yasuhiro Maeda, Kosuke Hashimoto, Bibin B. Andriana, Hiroko Matsuyoshi, Hidetoshi Sato
Summary: The study introduces a new type of CARS instrument with a double-wavelength-emission, background-free, electronically tuned Ti:sapphire laser (DW-ETL), which can provide stable CARS spectra for biological applications by controlling each laser pulse emission throughout the fingerprint region.
APPLIED SPECTROSCOPY
(2021)
Review
Oncology
Jiabao Xu, Tong Yu, Christos E. Zois, Ji-Xin Cheng, Yuguo Tang, Adrian L. Harris, Wei E. Huang
Summary: Raman spectroscopy and imaging are label-free, non-destructive techniques used to study cellular metabolism at subcellular level. This review summarizes the applications of Raman-based methods in cancer metabolism and imaging, highlighting their contribution to understanding complex biological processes.
Article
Multidisciplinary Sciences
Yujia Zhang, Minjian Lu, Zhendong Zhu, Yan Li, Haoyun Wei
Summary: Based on the enhancement factor of broadband SECARS excitation process, a novel Fano resonance plasmonic nanostructure is studied, which shows significant enhancement effect in a wide wavenumber region, covering most of the fingerprint region. This geometrically-tunable Fano plasmonic nanostructure provides a way to realize broadband-enhanced CARS, with potentials in single-molecular monitoring and high-selectivity biochemical detection.
SCIENTIFIC REPORTS
(2023)
Article
Thermodynamics
Daniel R. Richardson, Sean P. Kearney, Daniel R. Guildenbecher
Summary: Accurate measurement of post-detonation fireball temperatures is crucial for understanding device performance. In this study, a hybrid fs-ps CARS instrument was used to measure temperatures in the fireball of a commercial detonator. Challenges such as light scattering from particulate matter and solid fragments were addressed using a new polarization scheme.
PROCEEDINGS OF THE COMBUSTION INSTITUTE
(2021)
Review
Chemistry, Physical
Jasper J. van Thor, Paul M. Champion
Summary: This paper reviews the photoacid dynamics of fluorescent proteins, including both electronic excited- and ground-state mechanisms of proton transfer. It discusses the characteristic timescales of these reactions and the involvement of coherent nuclear motion. The paper also summarizes the experiments and theory regarding proton tunneling in the electronic ground state and excited-state proton-transfer reaction of the green fluorescent protein (GFP), as well as the remaining questions and recent advances.
ANNUAL REVIEW OF PHYSICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Lei Ouyang, Tobias Meyer-Zedler, Kel-Meng See, Wei-Liang Chen, Fan-Cheng Lin, Denis Akimov, Sadaf Ehtesabi, Martin Richter, Michael Schmitt, Yu-Ming Chang, Stefanie Grafe, Jurgen Popp, Jer-Shing Huang
Summary: This study investigates the enhancement effect of plasmonic gratings in nonlinear surface-enhanced coherent anti-Stokes Raman scattering (SECARS) using the platform of plasmonic Doppler grating (PDG). The PDGs spatially separate the input and output enhancement, allowing for observation and comparison of overall enhancement from different combinations of individual input and output beams. The results indicate that enhancement in the pump and anti-Stokes beams plays a more critical role in the overall enhancement than that in the Stokes beam.
Article
Instruments & Instrumentation
Runfeng Li, Ruijun Lan, Dashan Dong, Hong Yang, Kebin Shi
Summary: The Bessel beam CARS spectroscopic method enhances spectral fidelity and signal-to-noise ratio in a turbulent environment, improving the measurement accuracy.
APPLIED SPECTROSCOPY
(2023)
Article
Spectroscopy
Jakub Dostal
Summary: Coherent electronic two-dimensional spectroscopy is an established experimental technique for monitoring the time evolution of the studied sample after resonant optical excitation. It has been found that even nonresonant interactions can provide detectable spectral contributions. The adaptation of double-sided Feynman diagrams in this work provides a framework for studying various optical effects in the 2D spectrum.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Optics
Danni Chen, Zhengyi Liu, Shuanglong Liu, Hanben Niu
Summary: This article demonstrates three-color ultrabroadband CARS spectroscopy based on a single fiber laser. A supercontinuum with a wide bandwidth of 780-1240 nm is generated in a simple manner, allowing real-time capture of CARS signals in most of the fingerprint range.
OPTICS COMMUNICATIONS
(2022)
Article
Nanoscience & Nanotechnology
Tianjian Lv, Bing Han, Ming Yan, Zhaoyang Wen, Kun Huang, Kangwen Yang, Heping Zeng
Summary: This article introduces a hybrid dual-comb CARS system that uses a broadband fiber laser and a highly flexible, frequency-modulated electro-optic comb to achieve high-speed spectroscopic imaging. The spectral measurement speed in the high-wavenumber C-H stretching region is an order of magnitude higher than that of state-of-the-art systems, opening up new opportunities for fast chemical sensing and imaging.
Article
Cell Biology
Damien Boildieu, Tiffany Guerenne-Del Ben, Ludovic Duponchel, Vincent Sol, Jean-Michel Petit, Eric Champion, Hideaki Kano, David Helbert, Amandine Magnaudeix, Philippe Leproux, Philippe Carre
Summary: In this study, the combination of MCARS imaging technique with unsupervised data analysis based on multivariate curve resolution (MCR) is introduced for hyperspectral cell imaging and segmentation. The robustness, applicability, and sensitivity of this method are evaluated through experiments, and imaging results of different cell types and states are presented.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Dermatology
Roland Aschoff, Awena Lang, Edmund Koch
Summary: Topical corticosteroids (TCSs) are commonly used for the treatment of chronic inflammatory diseases, but skin atrophy is a concerning side effect. This study found that methylprednisolone aceponate, hydrocortisone acetate, and pimecrolimus may be more suitable for long-term treatment.
SKIN PHARMACOLOGY AND PHYSIOLOGY
(2022)
Article
Neurosciences
Martin Oelschlaegel, Witold H. Polanski, Ute Morgenstern, Gerald Steiner, Matthias Kirsch, Edmund Koch, Gabriele Schackert, Stephan B. Sobottka
Summary: The study investigated temporal characteristics of cortical hemodynamic responses following different stimulation paradigms, finding decreased CBV and increased HbR in the contralateral primary sensory cortex with electrical stimulation, while prolonged tactile and visual stimulation led to increased CBV in the corresponding activated areas (SI, visual cortex). The findings suggest that the type and paradigm of stimulation have a significant impact on the temporal characteristics of hemodynamic responses.
HUMAN BRAIN MAPPING
(2022)
Article
Biochemical Research Methods
Grit Preusse, Vincenz Porstmann, Thomas Bartels, Christian Schnabel, Roberta Galli, Edmund Koch, Martin Oelschlaegel, Ortrud Uckermann, Gerald Steiner
Summary: A novel method of in ovo sex determination using two-wavelength fluorescence excitation is described. The accuracy of sex determination is 96% with no reduction in hatching rate.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Clinical Neurology
Witold H. Polanski, Martin Oelschlagel, Tareq A. Juratli, Hannes Wahl, Pawel M. Krukowski, Ute Morgenstern, Edmund Koch, Gerald Steiner, Gabriele Schackert, Stephan B. Sobottka
Summary: This study presents a novel approach using intraoperative optical imaging (IOI) with painless tactile irritation to map different body regions within the primary sensory cortex (PSC). The results showed reliable changes in cerebral blood volume during IOI with tactile irritation, and a plausible topographic distribution along the PSC. The approach is suitable for awake surgeries and congruent to preoperative functional MRI (fMRI) findings.
Article
Oncology
Gerald Steiner, Roberta Galli, Grit Preusse, Susanne Michen, Matthias Meinhardt, Achim Temme, Stephan B. Sobottka, Tareq A. Juratli, Edmund Koch, Gabriele Schackert, Matthias Kirsch, Ortrud Uckermann
Summary: In this study, infrared spectroscopy was used to identify brain tumors. The classification model developed based on IR spectra successfully classified 82% of tumor and non-tumor samples, with high accuracy for different types of brain tumors. This approach could accelerate the clinical translation of infrared spectroscopy and enable the detection of multiple brain tumor types using a single instrument in future clinical settings.
JOURNAL OF NEURO-ONCOLOGY
(2023)
Article
Multidisciplinary Sciences
Jiachen Wu, Tijue Wang, Ortrud Uckermann, Roberta Galli, Gabriele Schackert, Liangcai Cao, Juergen Czarske, Robert Kuschmierz
Summary: Recent advances in label-free histology have the potential to revolutionize real-time diagnosis in neurosurgery. Deep learning using autofluorescence shows promise for tumor classification without the need for histochemical staining. The high image resolution and minimally invasive diagnostics with minimal tissue damage are critical factors.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Steven Melcher, Cordelia Zimmerer, Roberta Galli, Jonas Golde, Robert Herber, Frederik Raiskup, Edmund Koch, Gerald Steiner
Summary: Corneal cross-linking (CXL) with riboflavin and ultraviolet A light is a therapeutic procedure to restore the mechanical stability of corneal tissue. This study investigated molecular alterations within porcine cornea tissue after treatment with riboflavin and ultraviolet A light using surface enhanced Raman spectroscopy (SERS). The analysis revealed that while there was no general change in collagen secondary structure, modifications on amino acid side chains were the most dominant outcome. The formation of secondary and aromatic amine groups, as well as methylene and carbonyl groups, were observed.
Article
Biochemical Research Methods
Rimante Bandzeviciute, Gerald Steiner, Katja Liedel, Jonas Golde, Edmund Koch, Thilo Welsch, Christoph Kahlert, Daniel E. Stange, Marius Distler, Jurgen Weitz, Justinas Ceponkus, Valdas Sablinskas, Christian Teske
Summary: This study demonstrates the application of fiber-based attenuated total reflection infrared (ATR IR) spectroscopy for label-free discrimination of normal pancreatic, tumorous, and pancreatitis tissue. A classification model was established for differentiating between the distinct tissue classes using unprocessed freshly resected tissue samples of 58 patients. The method was further translated into intraoperative application, obtaining fiber optic ATR IR spectra directly in the operating room from freshly resected pancreatic tissue.
JOURNAL OF BIOMEDICAL OPTICS
(2023)
Article
Multidisciplinary Sciences
Roberta Galli, Tiziana Siciliano, Daniela Aust, Sandra Korn, Katrin Kirsche, Gustavo. B. B. Baretton, Juergen Weitz, Edmund Koch, Carina Riediger
Summary: As resection margins play a crucial role in predicting outcomes after colorectal liver metastases surgery, surgeons strive to obtain negative margins by resecting positive resection planes identified through intraoperative frozen sections. However, this is time-consuming and surgical outcomes can still be uncertain. In this study, label-free multimodal multiphoton microscopy (MPM) was used to obtain real-time morpho-chemical information of colorectal liver metastases and hepatic tissue without the need for staining. The results showed that MPM can accurately distinguish normal liver tissue from metastatic tissue and determine tumor borders in cryosections and formalin-fixed bulk tissue. Furthermore, automated tissue type classification using a simple approach based on texture parameters achieved a correct classification rate of nearly 95%. Therefore, MPM has the potential to improve resection margin precision in hepatic surgery for metastases without prolonging the surgical procedure.
SCIENTIFIC REPORTS
(2023)
Article
Medicine, General & Internal
Yuliia Gruda, Marius Albrecht, Michaela Buckova, Dominik Haim, Guenter Lauer, Edmund Koch, Korinna Joehrens, Christian Schnabel, Jonas Golde, Jiawen Li, Robert A. Mclaughlin, Julia Walther
Summary: This study evaluated the application of optical coherence tomography (OCT) in oral lichen planus (OLP). The results show that OCT provides more information about OLP variants, allowing for a more accurate localization of the OLP center and providing decision support for future biopsies.
Proceedings Paper
Engineering, Biomedical
Rimante Bandzeviciute, Gerald Steiner, Katja Liedel, Jonas Golde, Edmund Koch, Thilo Welsch, Christoph Kahlert, Daniel Stange, Marius Distler, Jurgen Weitz, Justinas Ceponkus, Valdas Sablinskas, Christian Teske
Summary: This study demonstrates the application of fiber-based attenuated total reflection infrared (ATR IR) spectroscopy for label-free discrimination of normal pancreatic, tumorous, and pancreatitis tissue. The method was successfully applied in the operating room for rapid tissue identification and showed potential for intraoperative surgical diagnostics.
ADVANCED BIOMEDICAL AND CLINICAL DIAGNOSTIC AND SURGICAL GUIDANCE SYSTEMS XXI
(2023)
Article
Engineering, Multidisciplinary
Anita Kubiak, Alona Voronkina, Martyna Pajewska-Szmyt, Martyna Kotula, Bartosz Lesniewski, Alexander Ereskovsky, Korbinian Heimler, Anika Rogoll, Carla Vogt, Parvaneh Rahimi, Sedigheh Falahi, Roberta Galli, Enrico Langer, Maik Foerste, Alexandros Charitos, Yvonne Joseph, Hermann Ehrlich, Teofil Jesionowski
Summary: In this study, spongin from Hippospongia communis was used as a template to develop a new 3D composite containing goethite. The obtained composite showed potential industrial applications and exhibited satisfactory performance in the electrochemical sensing of dopamine in human urine samples.
Article
Multidisciplinary Sciences
Jan Rix, Ortrud Uckermann, Katrin Kirsche, Gabriele Schackert, Edmund Koch, Matthias Kirsch, Roberta Galli
Summary: The study combined Brillouin spectroscopy and Raman spectroscopy to investigate the relationship between biomechanical properties and biochemical composition in U87-MG human glioblastoma cells at the subcellular level. The results showed that the nucleus and nucleoli are stiffer than the perinuclear region and the cytoplasm, and culturing conditions affect the biomechanics of the cell cytoplasm.
JOURNAL OF THE ROYAL SOCIETY INTERFACE
(2022)