期刊
JOURNAL OF BIOMEDICAL OPTICS
卷 21, 期 8, 页码 -出版社
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.JBO.21.8.084001
关键词
Flexible endoscope; fluorescence; Barrett's esophagus; clinical translation; near-infrared; dysplasia
资金
- EPSRC-CRUK Cancer Imaging Centre in Cambridge and Manchester [C197/A16465]
- CRUK [C14303/A17197]
- EU [FP7-PEOPLE-2013-CIG-630729]
- University of Cambridge MRC Confidence in Concept Award
- CRUK Career Establishment Award [C47594/A16267]
- MRC [MC_PC_13059] Funding Source: UKRI
- Cancer Research UK [17242, 21142, 16465, 16267] Funding Source: researchfish
- Medical Research Council [MC_PC_13059] Funding Source: researchfish
- National Institute for Health Research [NIHR-RP-02-12-011] Funding Source: researchfish
Barrett's esophagus is a known precursor lesion to esophageal adenocarcinoma. In these patients, early detection of premalignant disease, known as dysplasia, allows curative minimally invasive endoscopic therapy, but is confounded by a lack of contrast in white light endoscopy. Imaging fluorescently labeled lectins applied topically to the tissue has the potential to more accurately delineate dysplasia, but tissue autofluorescence limits both sensitivity and contrast when operating in the visible region. To overcome this challenge, we synthesized near-infrared (NIR) fluorescent wheat germ agglutinin (WGA-IR800CW) and constructed a clinically translatable bimodal NIR and white light endoscope. Images of NIR and white light with a field of view of 63 deg and an image resolution of 182 mu m are coregistered and the honeycomb artifact arising from the fiber bundle is removed. A minimum detectable concentration of 110 nM was determined using a dilution series of WGA-IR800CW. We demonstrated ex vivo that this system can distinguish between gastric and squamous tissue types in mouse stomachs (p = 0.0005) and accurately detect WGA-IR800CW fluorescence in human esophageal resections (compared with a gold standard imaging system, r(s) > 0.90). Based on these findings, future work will optimize the bimodal endoscopic system for clinical trials in Barrett's surveillance. (C) 2016 Society of Photo-Optical Instrumentation Engineers (SPIE)
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