期刊
ADVANCED SCIENCE
卷 9, 期 18, 页码 -出版社
WILEY
DOI: 10.1002/advs.202104463
关键词
hyperuricemia; indicator displacement assay; noninvasive diagnosis; smartphone; uric acid
资金
- National Natural Science Foundation of China [81873192]
- Tianjin Municipal Science and Technology Bureau [20ZYJDJC00070]
- National Administration of Traditional Chinese Medicine [ZYYCXTD-D-202002]
A high-throughput and visual detection method is provided for alarming hyperuricemia by noninvasive diagnosis.
Characterized by an excessively increased uric acid (UA) level in serum, hyperuricemia induces gout and also poses a great threat to renal and cardiovascular systems. It is urgent and meaningful to perform early warning by noninvasive diagnosis, thus conducing to blockage of disease aggravation. Here, guanidinocalix[5]arene (GC5A) is successfully identified from the self-built macrocyclic library to specifically monitor UA from urine by the indicator displacement assay. UA is strongly bound to GC5A at micromolar-level, while simultaneously excluding fluorescein (Fl) from the GC5A center dot Fl complex in the switch-on mode. This method successfully differentiates patients with hyperuricemia from volunteers except for those with kidney dysfunction and targets a volunteer at high risk of hyperuricemia. In order to meet the trend from hospital-centered to individual-centered testing, visual detection of UA is studied through a smartphone equipped with a color-scanning feature, whose adaptability and feasibility are demonstrated in sensing UA from authentic urine, leading to a promising method in family-centered healthcare style. A high-throughput and visual detection method is provided here for alarming hyperuricemic by noninvasive diagnosis.
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