Article
Entomology
Qi Wang, Yu-Tong Luo, Yong Wang, De-Yi Wang, Xiao-Xia Duan, Yao-Ting Zhang, Yu-Meng Bian, Wei Liu, Li Qin
Summary: This study used a long photoperiod to trigger pupal diapause termination in the Chinese oak silkworm. It examined the expression patterns of hormone-related genes, revealing a close relationship between hormone gene expression and pupal development.
Article
Agronomy
Zhi-Xiong Zhou, Wei Dou, Chuan-Ren Li, Jin-Jun Wang
Summary: The insect steroid hormone 20-hydroxyecdysone (20E) plays a crucial role in the induction and termination phase of obligatory diapause in Bactrocera minax by regulating energy metabolism. The expression of CYP314A1 gene regulates the biosynthesis of 20E, which is essential for pupal development and diapause in insects.
PEST MANAGEMENT SCIENCE
(2022)
Article
Physiology
Qi Wang, Liang Zhong, Yong Wang, Shengwei Zheng, Yumeng Bian, Junhao Du, Ruisheng Yang, Wei Liu, Li Qin
Summary: Melanism plays important roles in defense, immunoreactions, and body color in insects. During the larval-pupal metamorphosis of the Chinese oak silkworm, the expression of key genes TH and DDC, involved in dopamine synthesis, is regulated, affecting pupal melanization.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Philip Suess, Heinrich Dircksen, Kevin T. Roberts, Karl Gotthard, Dick R. Nassel, Christopher W. Wheat, Mikael A. Carlsson, Philipp Lehmann
Summary: Diapause is a crucial mechanism for insects to adapt their life cycle to adverse conditions, with key regulators being ecdysone and PTTH. Termination of diapause under low temperatures may occur precociously.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2022)
Article
Agronomy
Can Zhao, Yue Xia, Jun-Jian Xiao, Zi-Xin Liu, Zhang Bao-Xin, Dun-Song Li
Summary: This study investigated the biological parameters of diapaused and non-diapaused Anastatus japonicus after long-term storage in the eggs of the host Antheraea pernyi. The results showed that diapaused larvae were more cold-tolerant than non-diapaused larvae. Diapause induction enhanced the lifespan, fecundity, and oviposition period of Anastatus japonicus, but prolonged refrigeration significantly reduced its fecundity and oviposition period. The induction of diapause is an applicable technique for mass production of Anastatus japonicus in long-term storage.
PEST MANAGEMENT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Xin Fu, Miaomiao Chen, Runxi Xia, Xinyu Li, Qun Li, Yuping Li, Huiying Cao, Yanqun Liu
Summary: In this study, the cuticular proteins (CPs) in the genome of Antheraea pernyi were identified and compared with those of Bombyx mori. It was found that A. pernyi expressed more RR-2 genes during the pupal stage compared to B. mori, but expressed less RR-2 genes in the prothoracic gland. This suggests that the difference in hardness between the pupal skin and prothoracic gland of the two species may be caused by the number of expressed RR-2 genes. Additionally, it was revealed that B. mori expressed a higher number of CP genes in the antennae and prothoracic gland of the fifth instar larva than in the larval epidermis. This study provides an overall framework for functional research on CP genes in Saturniidae.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Guobao Wang, Jiangrun Wang, Lei Nie
Summary: This study employed RNA-seq to investigate the transcriptome profile of the central nervous system (CNS) of Antheraea pernyi, revealing the neuropeptides and neuropeptide receptors involved in regulating various behaviors and physiological processes. Comparison of gene expression patterns between the brain and ventral nerve cord (VNC) showed higher expression levels in the brain. Additionally, differentially expressed genes were screened and analyzed, providing a foundation for further research into their functions.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Agriculture, Multidisciplinary
T. Jiang, X. -Y. Li, M. -M. Chen, S. -T Liu, Y. -P. Li, R. -X. Xia, Y. -R. Jiang, Q. Li, Y. -Q. Liu
Summary: In this study, candidate chemosensory genes were characterized from the genomes of two wild silkmoths. The study found that the number of genes related to olfaction and taste varied between the two species. Furthermore, most of these genes were expressed in the antennal transcriptome, while some gustatory receptor genes showed no expression.
JOURNAL OF INSECTS AS FOOD AND FEED
(2023)
Article
Agronomy
Meijiao Yang, Guoqiang Li, Lu Yu, Shijie Du, Di Jiang, Xu Chu, Kai Wang, Songqing Wu, Rong Wang, Feiping Zhang, Xia Hu
Summary: This study investigates the regulatory pathways and signals involved in diapause termination in Monochamus alternatus larvae. The findings reveal that high temperature and metal ions play a role in diapause termination by affecting hormone biosynthesis and metabolism. Specifically, the genes CYP314a1 and JHEH are involved in 20E and JH biosynthesis and degradation, and their dysfunction leads to changes in diapause duration. This research provides insights into the molecular mechanisms regulating diapause termination and could contribute to the prevention and control of M. alternatus infestation.
PEST MANAGEMENT SCIENCE
(2023)
Article
Entomology
Saima Kausar, Ruochen Liu, Isma Gul, Muhammad Nadeem Abbas, Hongjuan Cui
Summary: DNA methylation has been found to play a crucial role in regulating gene expression in insects, especially in the innate immune system. Microbial infection may enhance host genome DNA methylation to evade host immunity.
Article
Biotechnology & Applied Microbiology
Huili Qiao, Jingya Wang, Yuanzhuo Wang, Juanjuan Yang, Bofan Wei, Miaomiao Li, Bo Wang, Xiaozhe Li, Yang Cao, Ling Tian, Dandan Li, Lunguang Yao, Yunchao Kan
Summary: This study identified and characterized lncRNAs associated with 20E-induced autophagy in the fat body of B. mori. The lncRNA LNC_000560 and its potential target gene AtG4B were found to be related to 20E-regulated autophagy in B. mori, providing insights for further research on the regulatory mechanisms of lncRNAs in autophagy and other biological processes in this insect model.
Article
Entomology
Wei-Nan Kang, Bing-Yao Wang, Kai-Yun Fu, Wen-Chao Guo, Lin Jin, Guo-Qing Li
Summary: FoxO protein is crucial for the regulation of larval growth and metamorphosis in Leptinotarsa decemlineata, interacting with hormonal signaling pathways to control development.
JOURNAL OF INSECT PHYSIOLOGY
(2021)
Article
Entomology
Jianping Duan, Shanshan Li, Zhengtian Zhang, Lunguang Yao, Xinfeng Yang, Sanyuan Ma, Nini Duan, Jiazhen Wang, Xuwei Zhu, Ping Zhao
Summary: This study obtained the complete transcriptome information of the silk gland in Antheraea pernyi, including 12,572 high-confidence isoforms and 7,658 genes, with over 40% of genes not yet annotated in the reference genome. Furthermore, about 9% of isoforms were identified as long non-coding RNAs. Additionally, the study revealed numerous alternative splicing and alternative polyadenylation events, as well as the identification of putative transcription factors in A. pernyi genome. These findings greatly enhance our understanding of gene expression in the silk gland and provide valuable resources for further research on silk protein synthesis, spinning, genetic improvement, and non-traditional applications in A. pernyi.
JOURNAL OF ASIA-PACIFIC ENTOMOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Zhi-xiong Zhou, Xiao-lin Dong, Chuan-ren Li
Summary: This study investigated the molecular mechanisms of obligatory diapause induction in the Chinese citrus fruit fly using high-throughput RNA-Seq data. Differentially expressed genes related to diapause induction were identified, with functions in 20E biosynthesis, cell cycle, and metabolic pathways. These findings provide important insights into the molecular basis of diapause induction in this pest species.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
(2021)
Article
Biochemistry & Molecular Biology
Hai-Chao Wang, Hai-Bin Han, Tian-Feng Duan, Ling Li, Bao-Ping Pang
Summary: This study found that 20E may influence the metabolic changes during reproductive diapause in G. daurica by regulating miRNAs.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
(2022)